LOCUS       Exported                5742 bp ds-DNA     circular SYN 01-AUG-2016
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    pEZ5
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5742)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Aug 4, 2016 from SnapGene 3.1.4
            http://www.snapgene.com
COMMENT     >pAQ1_YFP
FEATURES             Location/Qualifiers
     source          1..5742
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    545..1450
                     /note="Bla AmpR"
     misc_feature    1010..1031
                     /note="back tetO Fwd"
     misc_feature    complement(1010..1031)
                     /note="back tetO Rev"
     misc_feature    1630..2249
                     /note="pBR322 ori"
     misc_feature    2461..2480
                     /note="Primer76"
     misc_feature    2687..3225
                     /note="acsA up"
     misc_feature    2687..2707
                     /note="acsA-UpF(v2)"
     misc_feature    2687..2695
                     /note="acsA-upF(v1)"
     misc_feature    3131..3150
                     /note="Primer ALM 70"
     misc_feature    3203..3225
                     /note="acsA-UpR [EcoRI]"
     terminator      3240..3259
     terminator      3300..3343
     gene            complement(3391..3427)
                     /note="ssRa"
     gene            complement(3428..4047)
                     /note="tetR"
     RBS             4054..4065
     misc_feature    4074..4120
                     /note="J23117 Promoter"
     misc_feature    complement(4090..4122)
                     /note="TetR (TetO2*) Ins Rev"
     misc_feature    4121..4201
                     /note="Promoter"
     misc_feature    4121..4128
                     /note="TetO2* Vec Fwd"
     misc_feature    4125..4141
                     /note="TetO1"
     -10_signal      4142..4144
                     /note="-35"
     misc_feature    4155..4171
                     /note="TetO1"
     -10_signal      4173..4178
                     /note="-10"
     misc_feature    4186
                     /note="transcription start site"
     misc_feature    4226..4231
                     /note="RBS"
     misc_feature    4240..4917
                     /note="Thermostable GFP"
     misc_feature    4971..4989
                     /note="T7 terminator"
     misc_feature    5014..5035
                     /note="pAQ1 into pACSA fix2"
     misc_feature    5022..5035
                     /note="Missing Terminator piece"
     misc_feature    5036..5529
                     /note="acsA down"
     misc_feature    5036..5059
                     /note="acsA-DownF [BamHI]"
     misc_feature    5508..5529
                     /note="acsA-DownR(v2)"
     misc_feature    5512..5529
                     /note="acsA-DownR(v1)"
     misc_feature    5725..5742
                     /note="Primer77"
ORIGIN
        1 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
       61 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
      121 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
      181 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
      241 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
      301 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct
      361 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa
      421 cgcttacaat ttcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg
      481 catcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
      541 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
      601 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
      661 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
      721 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
      781 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
      841 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
      901 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
      961 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     1021 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     1081 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     1141 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
     1201 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
     1261 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     1321 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     1381 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     1441 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     1501 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     1561 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     1621 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     1681 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     1741 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
     1801 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     1861 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     1921 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     1981 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     2041 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     2101 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     2161 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     2221 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     2281 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     2341 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
     2401 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
     2461 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa
     2521 ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc
     2581 gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg
     2641 attacgccaa gctatttagg tgacactata gaatactcaa gctatgaggt gactgccgca
     2701 ctcaatgttc aaccagatct agtgattatt tcggggttag cagccgatgc gggcaacctg
     2761 gtgaagcaac tgcgagaatt aggttacaac ggcattattg ttggggggaa cggcttaaat
     2821 acttctaata ttttccccgt ctgccaagca aaatgtgatg gggtgttggt ggcccaagcc
     2881 tacagtgccg agttagataa tgagattaac cgcgcgtttc gggacgccta ttttcaacaa
     2941 aaccaaaaag agccgcccca atttagtgcc caggctttta cggcgatcca agtttttgtt
     3001 gaagccctca gcagcctcga tgaaaaaacg cccttagaaa ctcttgctct accggacttg
     3061 cgacgacaac tgcgggacga aatttttgca ggtacctacg tcacgccttt gggtgaaatt
     3121 tccttcacag aggaagggga aattgtccag aaggaatttt ttgtggccca aattgaaatg
     3181 gatgaatcgg gtcaacaggg gcgtttcgcc ttcattgaaa cgaactataa acgcagaaag
     3241 gcccacccga aggtgagcca gtgtgactct agtagagagc gttcaccgac aaacaacaga
     3301 taaaacgaaa ggcccagtct ttcgactgag cctttcgttt tatttgatgc ctggctctag
     3361 tagtgatcta cactagcact atcagtgtta ttaagctact aaagcgtagt tttcgtcgtt
     3421 tgcagcggac ccactttcac atttaagttg tttttctaat ccgcatatga tcaattcaag
     3481 gccgaataag aaggctggct ctgcaccttg gtgatcaaat aattcgatag cttgtcgtaa
     3541 taatggcggc atactatcag tagtaggtgt ttccctttct tctttagcga cttgatgctc
     3601 ttgatcttcc aatacgcaac ctaaagtaaa atgccccaca gcgctgagtg catataatgc
     3661 attctctagt gaaaaacctt gttggcataa aaaggctaat tgattttcga gagtttcata
     3721 ctgtttttct gtaggccgtg tacctaaatg tacttttgct ccatcgcgat gacttagtaa
     3781 agcacatcta aaacttttag cgttattacg taaaaaatct tgccagcttt ccccttctaa
     3841 agggcaaaag tgagtatggt gcctatctaa catctcaatg gctaaggcgt cgagcaaagc
     3901 ccgcttattt tttacatgcc aatacaatgt aggctgctct acacctagct tctgggcgag
     3961 tttacgggtt gttaaacctt cgattccgac ctcattaagc agctctaatg cgctgttaat
     4021 cactttactt ttatctaatc tggacatcta gtatttctcc tctttctcta gtagctagca
     4081 caatccctag gactgagcta gctgtcaagg aattcttaac aaaatcccta tcagtgatag
     4141 aacaagatgt aacatcccta tcagtgatag agtataaagt taactgtggg attgcaaaag
     4201 gagctcccaa ctcataaagt caagtaggag attaatcata tgtcggtcat caagcccgaa
     4261 atgaaaatta aactgcgcat ggagggcgct gtgaacgggc acaagttcgt gatcgaaggt
     4321 gaaggcattg gtaagccgta tgaaggtact caaaccctgg acttaacggt taaagaaggt
     4381 gccccgttgc catttagcta cgacatcctg acacccgctt ttcagtatgg caatcgcgcg
     4441 ttcaccaagt atccgaaaga tattccggat tatttcaagc aggcattccc ggaaggatac
     4501 tcatgggaac gctcgatgac ctacgaagac cagggcattt gcattgccac aagcgacatc
     4561 acaatggagg gtgattgctt tttttacaaa attcgcttcg acgggacaaa cttcccgccg
     4621 aatggcccag tgatgcagaa aaagacgctt aaatgggaac cgtcgacaga gaaaatgtac
     4681 gttcgcgatg gcgttctgaa aggcgatgtt aatatggcgc tgcttctcga agggggaggc
     4741 cattaccgtt gtgatttcaa aaccacatac aaagcaaaaa aagatgttcg tttgccggac
     4801 gcgcatgaag tcgatcaccg cattgagatc ctgtcacatg ataaagatta caataaggtc
     4861 cgcctttatg agcacgctga ggcccgctat tctatgctgc ccagccaagc taaataagga
     4921 tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata
     4981 actagcataa ccccttgggg cctctaaacg ggtcttgacg ggttttttgt ctagagcgct
     5041 caagttgatc gaatgtatgc cccggatatg acctagggag attcttctaa agcgagcagg
     5101 gtttgccaag tggcgatcgc ctcagggaac cataaccaac tttgttcgag gcggcggggg
     5161 ttgagctggg aacgatcgag atctagggcg atcgcccgga gtttttcggc tttccctaca
     5221 agaatttcct ggtcaacgtc ggtgggttgc ctttgggcca aatcgtacat actaatcgcc
     5281 aggcccgcat aaatctccgc ctggagggct ggggggaaat cctgggtcgt tgccgccgct
     5341 aaggcttcat accaacgatc attggcggct tcgtaatctg cattgaggta atggacaaaa
     5401 cccagggcca tcaaaatttc ggggtcgttg ggctgttggg ctagggccat gtcccaattg
     5461 tcttgcaccg tggcgatcgc cgttgcttgg gttggcgatg gtgggacggc agccaaagtt
     5521 gtttgccagc atgcgcccta tagtgagtcg tattacaatt cactggccgt cgttttacaa
     5581 cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct
     5641 ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc
     5701 agcctgaatg gcgaatggac gcgccctgta gcggcgcatt aa
//
