LOCUS       Exported                5739 bp ds-DNA     circular SYN 01-AUG-2016
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    pEZ8
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5739)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Aug 4, 2016 from SnapGene 3.1.4
            http://www.snapgene.com
COMMENT     >pAQ1_YFP
FEATURES             Location/Qualifiers
     source          1..5739
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    545..1450
                     /note="Bla AmpR"
     misc_feature    1010..1031
                     /note="back tetO Fwd"
     misc_feature    complement(1010..1031)
                     /note="back tetO Rev"
     misc_feature    1630..2249
                     /note="pBR322 ori"
     misc_feature    2461..2480
                     /note="Primer76"
     misc_feature    2687..3225
                     /note="acsA up"
     misc_feature    2687..2707
                     /note="acsA-UpF(v2)"
     misc_feature    2687..2695
                     /note="acsA-upF(v1)"
     misc_feature    3131..3150
                     /note="Primer ALM 70"
     misc_feature    3203..3225
                     /note="acsA-UpR [EcoRI]"
     terminator      3240..3259
     terminator      3300..3343
     gene            complement(3391..3427)
                     /note="ssRa"
     gene            complement(3428..4047)
                     /note="tetR"
     RBS             4054..4065
     misc_feature    4074..4108
                     /note="J23113 Promoter"
     misc_feature    complement(4090..4128)
                     /note="TetR (TetO2*) Ins Rev"
     misc_feature    4109..4133
                     /note="TetO2* Vec Fwd"
     misc_feature    4110..4198
                     /note="Promoter"
     misc_feature    4130..4146
                     /note="TetO1"
     -10_signal      4147..4152
                     /note="-35"
     misc_feature    4153..4169
                     /note="TetO1"
     -10_signal      4170..4175
                     /note="-10"
     misc_feature    4183
                     /note="transcription start site"
     misc_feature    4223..4228
                     /note="RBS"
     misc_feature    4237..4914
                     /note="Thermostable GFP"
     misc_feature    4968..4986
                     /note="T7 terminator"
     misc_feature    5011..5032
                     /note="pAQ1 into pACSA fix2"
     misc_feature    5019..5032
                     /note="Missing Terminator piece"
     misc_feature    5033..5526
                     /note="acsA down"
     misc_feature    5033..5056
                     /note="acsA-DownF [BamHI]"
     misc_feature    5505..5526
                     /note="acsA-DownR(v2)"
     misc_feature    5509..5526
                     /note="acsA-DownR(v1)"
     misc_feature    5722..5739
                     /note="Primer77"
ORIGIN
        1 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
       61 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
      121 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
      181 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
      241 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
      301 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct
      361 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa
      421 cgcttacaat ttcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg
      481 catcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
      541 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
      601 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
      661 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
      721 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
      781 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
      841 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
      901 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
      961 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     1021 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     1081 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     1141 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
     1201 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
     1261 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     1321 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     1381 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     1441 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     1501 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     1561 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     1621 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     1681 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     1741 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
     1801 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     1861 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     1921 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     1981 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     2041 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     2101 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     2161 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     2221 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     2281 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     2341 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
     2401 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
     2461 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa
     2521 ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc
     2581 gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg
     2641 attacgccaa gctatttagg tgacactata gaatactcaa gctatgaggt gactgccgca
     2701 ctcaatgttc aaccagatct agtgattatt tcggggttag cagccgatgc gggcaacctg
     2761 gtgaagcaac tgcgagaatt aggttacaac ggcattattg ttggggggaa cggcttaaat
     2821 acttctaata ttttccccgt ctgccaagca aaatgtgatg gggtgttggt ggcccaagcc
     2881 tacagtgccg agttagataa tgagattaac cgcgcgtttc gggacgccta ttttcaacaa
     2941 aaccaaaaag agccgcccca atttagtgcc caggctttta cggcgatcca agtttttgtt
     3001 gaagccctca gcagcctcga tgaaaaaacg cccttagaaa ctcttgctct accggacttg
     3061 cgacgacaac tgcgggacga aatttttgca ggtacctacg tcacgccttt gggtgaaatt
     3121 tccttcacag aggaagggga aattgtccag aaggaatttt ttgtggccca aattgaaatg
     3181 gatgaatcgg gtcaacaggg gcgtttcgcc ttcattgaaa cgaactataa acgcagaaag
     3241 gcccacccga aggtgagcca gtgtgactct agtagagagc gttcaccgac aaacaacaga
     3301 taaaacgaaa ggcccagtct ttcgactgag cctttcgttt tatttgatgc ctggctctag
     3361 tagtgatcta cactagcact atcagtgtta ttaagctact aaagcgtagt tttcgtcgtt
     3421 tgcagcggac ccactttcac atttaagttg tttttctaat ccgcatatga tcaattcaag
     3481 gccgaataag aaggctggct ctgcaccttg gtgatcaaat aattcgatag cttgtcgtaa
     3541 taatggcggc atactatcag tagtaggtgt ttccctttct tctttagcga cttgatgctc
     3601 ttgatcttcc aatacgcaac ctaaagtaaa atgccccaca gcgctgagtg catataatgc
     3661 attctctagt gaaaaacctt gttggcataa aaaggctaat tgattttcga gagtttcata
     3721 ctgtttttct gtaggccgtg tacctaaatg tacttttgct ccatcgcgat gacttagtaa
     3781 agcacatcta aaacttttag cgttattacg taaaaaatct tgccagcttt ccccttctaa
     3841 agggcaaaag tgagtatggt gcctatctaa catctcaatg gctaaggcgt cgagcaaagc
     3901 ccgcttattt tttacatgcc aatacaatgt aggctgctct acacctagct tctgggcgag
     3961 tttacgggtt gttaaacctt cgattccgac ctcattaagc agctctaatg cgctgttaat
     4021 cactttactt ttatctaatc tggacatcta gtatttctcc tctttctcta gtagctagca
     4081 taatccctag gactgagcta gccatcaggt taacaaaaaa gcaggaatat ccctatcagt
     4141 gatagacaga catccctatc agtgatagat ataaagttaa ctgtgggatt gcaaaaggag
     4201 ctcccaactc ataaagtcaa gtaggagatt aatcatatgt cggtcatcaa gcccgaaatg
     4261 aaaattaaac tgcgcatgga gggcgctgtg aacgggcaca agttcgtgat cgaaggtgaa
     4321 ggcattggta agccgtatga aggtactcaa accctggact taacggttaa agaaggtgcc
     4381 ccgttgccat ttagctacga catcctgaca cccgcttttc agtatggcaa tcgcgcgttc
     4441 accaagtatc cgaaagatat tccggattat ttcaagcagg cattcccgga aggatactca
     4501 tgggaacgct cgatgaccta cgaagaccag ggcatttgca ttgccacaag cgacatcaca
     4561 atggagggtg attgcttttt ttacaaaatt cgcttcgacg ggacaaactt cccgccgaat
     4621 ggcccagtga tgcagaaaaa gacgcttaaa tgggaaccgt cgacagagaa aatgtacgtt
     4681 cgcgatggcg ttctgaaagg cgatgttaat atggcgctgc ttctcgaagg gggaggccat
     4741 taccgttgtg atttcaaaac cacatacaaa gcaaaaaaag atgttcgttt gccggacgcg
     4801 catgaagtcg atcaccgcat tgagatcctg tcacatgata aagattacaa taaggtccgc
     4861 ctttatgagc acgctgaggc ccgctattct atgctgccca gccaagctaa ataaggatcc
     4921 ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact
     4981 agcataaccc cttggggcct ctaaacgggt cttgacgggt tttttgtcta gagcgctcaa
     5041 gttgatcgaa tgtatgcccc ggatatgacc tagggagatt cttctaaagc gagcagggtt
     5101 tgccaagtgg cgatcgcctc agggaaccat aaccaacttt gttcgaggcg gcgggggttg
     5161 agctgggaac gatcgagatc tagggcgatc gcccggagtt tttcggcttt ccctacaaga
     5221 atttcctggt caacgtcggt gggttgcctt tgggccaaat cgtacatact aatcgccagg
     5281 cccgcataaa tctccgcctg gagggctggg gggaaatcct gggtcgttgc cgccgctaag
     5341 gcttcatacc aacgatcatt ggcggcttcg taatctgcat tgaggtaatg gacaaaaccc
     5401 agggccatca aaatttcggg gtcgttgggc tgttgggcta gggccatgtc ccaattgtct
     5461 tgcaccgtgg cgatcgccgt tgcttgggtt ggcgatggtg ggacggcagc caaagttgtt
     5521 tgccagcatg cgccctatag tgagtcgtat tacaattcac tggccgtcgt tttacaacgt
     5581 cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc
     5641 gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc
     5701 ctgaatggcg aatggacgcg ccctgtagcg gcgcattaa
//
