LOCUS       Exported                5740 bp ds-DNA     circular SYN 01-AUG-2016
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    pEZ14
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5740)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Aug 4, 2016 from SnapGene 3.1.4
            http://www.snapgene.com
COMMENT     >pAQ1_YFP
FEATURES             Location/Qualifiers
     source          1..5740
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    545..1450
                     /note="Bla AmpR"
     misc_feature    1010..1031
                     /note="back tetO Fwd"
     misc_feature    complement(1010..1031)
                     /note="back tetO Rev"
     misc_feature    1630..2249
                     /note="pBR322 ori"
     misc_feature    2461..2480
                     /note="Primer76"
     misc_feature    2687..3225
                     /note="acsA up"
     misc_feature    2687..2707
                     /note="acsA-UpF(v2)"
     misc_feature    2687..2695
                     /note="acsA-upF(v1)"
     misc_feature    3131..3150
                     /note="Primer ALM 70"
     misc_feature    3203..3225
                     /note="acsA-UpR [EcoRI]"
     terminator      3240..3259
     terminator      3300..3343
     gene            complement(3391..3427)
                     /note="ssRa"
     gene            complement(3428..4047)
                     /note="tetR"
     RBS             4054..4065
     misc_feature    4074..4108
                     /note="J23113 Promoter"
     misc_feature    complement(4090..4128)
                     /note="TetR (TetO2*) Ins Rev"
     misc_feature    4109..4133
                     /note="TetO2* Vec Fwd"
     misc_feature    4110..4199
                     /note="Promoter"
     misc_feature    4130..4146
                     /note="TetO1"
     -10_signal      4147..4152
                     /note="-35"
     misc_feature    4153..4169
                     /note="TetO1"
     -10_signal      4171..4175
                     /note="-10"
     misc_feature    4184
                     /note="transcription start site"
     misc_feature    4224..4229
                     /note="RBS"
     misc_feature    4238..4915
                     /note="Thermostable GFP"
     misc_feature    4969..4987
                     /note="T7 terminator"
     misc_feature    5012..5033
                     /note="pAQ1 into pACSA fix2"
     misc_feature    5020..5033
                     /note="Missing Terminator piece"
     misc_feature    5034..5527
                     /note="acsA down"
     misc_feature    5034..5057
                     /note="acsA-DownF [BamHI]"
     misc_feature    5506..5527
                     /note="acsA-DownR(v2)"
     misc_feature    5510..5527
                     /note="acsA-DownR(v1)"
     misc_feature    5723..5740
                     /note="Primer77"
ORIGIN
        1 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
       61 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
      121 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
      181 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
      241 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
      301 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct
      361 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa
      421 cgcttacaat ttcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg
      481 catcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
      541 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
      601 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
      661 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
      721 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
      781 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
      841 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
      901 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
      961 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     1021 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     1081 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     1141 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
     1201 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
     1261 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     1321 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     1381 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     1441 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     1501 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     1561 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     1621 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     1681 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     1741 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
     1801 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     1861 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     1921 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     1981 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     2041 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     2101 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     2161 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     2221 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     2281 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     2341 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
     2401 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
     2461 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa
     2521 ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc
     2581 gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg
     2641 attacgccaa gctatttagg tgacactata gaatactcaa gctatgaggt gactgccgca
     2701 ctcaatgttc aaccagatct agtgattatt tcggggttag cagccgatgc gggcaacctg
     2761 gtgaagcaac tgcgagaatt aggttacaac ggcattattg ttggggggaa cggcttaaat
     2821 acttctaata ttttccccgt ctgccaagca aaatgtgatg gggtgttggt ggcccaagcc
     2881 tacagtgccg agttagataa tgagattaac cgcgcgtttc gggacgccta ttttcaacaa
     2941 aaccaaaaag agccgcccca atttagtgcc caggctttta cggcgatcca agtttttgtt
     3001 gaagccctca gcagcctcga tgaaaaaacg cccttagaaa ctcttgctct accggacttg
     3061 cgacgacaac tgcgggacga aatttttgca ggtacctacg tcacgccttt gggtgaaatt
     3121 tccttcacag aggaagggga aattgtccag aaggaatttt ttgtggccca aattgaaatg
     3181 gatgaatcgg gtcaacaggg gcgtttcgcc ttcattgaaa cgaactataa acgcagaaag
     3241 gcccacccga aggtgagcca gtgtgactct agtagagagc gttcaccgac aaacaacaga
     3301 taaaacgaaa ggcccagtct ttcgactgag cctttcgttt tatttgatgc ctggctctag
     3361 tagtgatcta cactagcact atcagtgtta ttaagctact aaagcgtagt tttcgtcgtt
     3421 tgcagcggac ccactttcac atttaagttg tttttctaat ccgcatatga tcaattcaag
     3481 gccgaataag aaggctggct ctgcaccttg gtgatcaaat aattcgatag cttgtcgtaa
     3541 taatggcggc atactatcag tagtaggtgt ttccctttct tctttagcga cttgatgctc
     3601 ttgatcttcc aatacgcaac ctaaagtaaa atgccccaca gcgctgagtg catataatgc
     3661 attctctagt gaaaaacctt gttggcataa aaaggctaat tgattttcga gagtttcata
     3721 ctgtttttct gtaggccgtg tacctaaatg tacttttgct ccatcgcgat gacttagtaa
     3781 agcacatcta aaacttttag cgttattacg taaaaaatct tgccagcttt ccccttctaa
     3841 agggcaaaag tgagtatggt gcctatctaa catctcaatg gctaaggcgt cgagcaaagc
     3901 ccgcttattt tttacatgcc aatacaatgt aggctgctct acacctagct tctgggcgag
     3961 tttacgggtt gttaaacctt cgattccgac ctcattaagc agctctaatg cgctgttaat
     4021 cactttactt ttatctaatc tggacatcta gtatttctcc tctttctcta gtagctagca
     4081 taatccctag gactgagcta gccatcaggt taacaaaaaa gcaggaatat ccctatcagt
     4141 gatagacaga catccctatc agtgatagag tataatgtta actgtgggat tgcaaaagga
     4201 gctcccaact cataaagtca agtaggagat taatcatatg tcggtcatca agcccgaaat
     4261 gaaaattaaa ctgcgcatgg agggcgctgt gaacgggcac aagttcgtga tcgaaggtga
     4321 aggcattggt aagccgtatg aaggtactca aaccctggac ttaacggtta aagaaggtgc
     4381 cccgttgcca tttagctacg acatcctgac acccgctttt cagtatggca atcgcgcgtt
     4441 caccaagtat ccgaaagata ttccggatta tttcaagcag gcattcccgg aaggatactc
     4501 atgggaacgc tcgatgacct acgaagacca gggcatttgc attgccacaa gcgacatcac
     4561 aatggagggt gattgctttt tttacaaaat tcgcttcgac gggacaaact tcccgccgaa
     4621 tggcccagtg atgcagaaaa agacgcttaa atgggaaccg tcgacagaga aaatgtacgt
     4681 tcgcgatggc gttctgaaag gcgatgttaa tatggcgctg cttctcgaag ggggaggcca
     4741 ttaccgttgt gatttcaaaa ccacatacaa agcaaaaaaa gatgttcgtt tgccggacgc
     4801 gcatgaagtc gatcaccgca ttgagatcct gtcacatgat aaagattaca ataaggtccg
     4861 cctttatgag cacgctgagg cccgctattc tatgctgccc agccaagcta aataaggatc
     4921 cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac
     4981 tagcataacc ccttggggcc tctaaacggg tcttgacggg ttttttgtct agagcgctca
     5041 agttgatcga atgtatgccc cggatatgac ctagggagat tcttctaaag cgagcagggt
     5101 ttgccaagtg gcgatcgcct cagggaacca taaccaactt tgttcgaggc ggcgggggtt
     5161 gagctgggaa cgatcgagat ctagggcgat cgcccggagt ttttcggctt tccctacaag
     5221 aatttcctgg tcaacgtcgg tgggttgcct ttgggccaaa tcgtacatac taatcgccag
     5281 gcccgcataa atctccgcct ggagggctgg ggggaaatcc tgggtcgttg ccgccgctaa
     5341 ggcttcatac caacgatcat tggcggcttc gtaatctgca ttgaggtaat ggacaaaacc
     5401 cagggccatc aaaatttcgg ggtcgttggg ctgttgggct agggccatgt cccaattgtc
     5461 ttgcaccgtg gcgatcgccg ttgcttgggt tggcgatggt gggacggcag ccaaagttgt
     5521 ttgccagcat gcgccctata gtgagtcgta ttacaattca ctggccgtcg ttttacaacg
     5581 tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt
     5641 cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag
     5701 cctgaatggc gaatggacgc gccctgtagc ggcgcattaa
//
