LOCUS       498pDestTol2pA2         7006 bp ss-DNA     circular SYN 01-Feb-2012
DEFINITION  -
ACCESSION   -
KEYWORDS    -
SOURCE      -
FEATURES             Location/Qualifiers
     misc_feature    462..513
                     /label=backbone"
                     /note=""
     misc_feature    514..1052
                     /label=Tol2 R arm"
                     /note=""
     misc_signal     complement(514..1021)
                     /note="Tol2 right arm"
     frag            514..1021
                     /note="659 to 1166 of Kozak_G2AC_in_pBT2"
     primer_bind     919..938
                     /note="cma5"
     primer_bind     1061..1082
                     /note="M13 R"
     misc_recomb     1097..>1103
                     /note="attR4"
     LTR             1103
                     /note="43 LR start"
     misc_difference 1118..1211
     frag            1118..1211
                     /note="688 to 781 of 1078.P5'(QUAS)"
     frag            join(<1212..>1332,<2325..>2347,<3902..>3931)
                     /note="669 to 2500 of Untitled [Split]"
     frag            <1212..1308
                     /note="688 to 878 of 1078.P5'(QUAS)"
     misc_difference 1212..1308
     frag            1335..2323
                     /date="11-Jul-2016"
                     /note="686 to 1674 of 1063 ME GFP pA in bkbn"
     frag            <1335..>2323
                     /note="748 to 1768 of ME GFP pA [Split]"
     frag            <1335..2323
                     /note="748 to 1741 of 493 p3E-EGFPpA [Split]"
     misc_feature    <1335..1347
                     /note="gtwy F ME [Split]"
     misc_feature    1335..2051
                     /note="eGFP"
     misc_feature    2052..2111
                     /note="pCS2 MCS frag"
     misc_feature    2128..2323
                     /note="SV40 late polyA"
     misc_feature    2306..2323
                     /note="gtwy R"
     frag            2349..>2350
                     /note="2304 to 3373 of pDestT2 QUASp-Arch3-2amCherrycaaxpA"
     misc_feature    2349..>2350
                     /label=PTV1-2A peptide"
                     /note="/ApEinfo_fwdcolor=#004fff; /ApEinfo_revcolor=cyan"
                     /note=""
     frag            2349..>2350
                     /note="17 to 82 of 966 p3E-2A-mCherryCAAXpA.ape"
     misc_feature    2351..3901
     frag            2351..3901
                     /date="11-Jul-2016"
                     /note="2138 to 3688 of 1107.QUAS.mAppleCAAX.BcrysCFP"
     primer_bind     3932..3947
                     /note="M13 F"
     polyA_signal    3963..4160
                     /note="polyA"
     polyA_signal    3967..4188
                     /note="SV40 late polyA region"
     polyA_signal    complement(3967..4097)
                     /note="SV40 polyadenylation signal"
     misc_signal     4219..4736
                     /note="Tol2 left arm"
     misc_feature    4219..4736
                     /label=Tol2 L arm"
                     /note=""
     frag            4219..4736
                     /note="3005 to 3522 of Kozak_G2AC_in_pBT2"
     LTR             4219..4553
                     /note="tol2 exon1"
     LTR             4343..4362
                     /note="cma 103"
     LTR             4717..4735
                     /note="Tol2 L"
     LTR             4717..4735
                     /note="Tol2 L"
     promoter        4821..4840
                     /note="T3"
     misc_feature    complement(4851..5080)
                     /label=M13 REverse"
                     /note=""
     misc_feature    4851..5098
                     /note="lac operon sequence"
     primer_bind     4856..4877
                     /note="M13R Complement"
     primer_bind     complement(4861..4882)
                     /note="pUC"
                     /note="/M13 reverse sequencing primer"
     promoter        complement(4866..4987)
                     /note="Plac"
     primer_bind     complement(4874..4897)
                     /note="Ptac forward sequencing primer (NEB #1260,
                     discontinued)"
     protein_bind    complement(4881..4905)
                     /bound_moiety="LacI"
                     /note="lac operator"
     misc_feature    complement(5080..5143)
                     /citation=[5]
                     /note="L-strand Y effector site"
     rep_origin      5245..5274
                     /note="ColE1-derived plasmid replication origin"
     rep_origin      5260..5296
                     /note="ColE1-derived origin of replication"
     misc_RNA        complement(5263..5815)
                     /note="RNAII transcript (complementary strand)"
     rep_origin      5263..5851
                     /note="pMB1 origin of replication (counterclockwise)
                     (RNAII -35 to RNA"
                     /note="/DNA switch point)"
     rep_origin      complement(5263..5851)
                     /note="pMB1 origin of replication (clockwise) (RNAII -35
                     to RNA"
                     /note="/DNA switch point)"
     misc_RNA        5705..5812
                     /note="RNAI transcript"
     repeat_region   5877..5914
                     /note="corresponds to one of the 38bp repeats found in Tn3
                     (bp 1-38 and complement (4920-4957))"
                     /rpt_type=inverted
     CDS             complement(6022..6882)
                     /codon_start=1
                     /db_xref="GI:455370"
                     /gene="bla"
                     /note="E-286"
                     /product="beta-lactamase"
                     /protein_id="AAB59737.1"
                     /transl_table=11
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            complement(6022..6882)
                     /gene="bla"
     CDS             6025..6882
                     /note="ampR"
     mat_peptide     complement(6025..6813)
                     /gene="bla"
                     /product="beta-lactamase"
     sig_peptide     complement(6814..6882)
                     /gene="bla"
                     /note="Required for secretion to the periplasm"
                     /note="cleaved off to form the mature beta-lactamase
                     protein."
BASE COUNT      1933 A      1730 C      1622 G      1719 T         0 OTHER
ORIGIN      ?
        1 CCACCTAAAT TGTAAGCGTT AATATTTTGT TAAAATTCGC GTTAAATTTT TGTTAAATCA
       61 GCTCATTTTT TAACCAATAG GCCGAAATCG GCAAAATCCC TTATAAATCA AAAGAATAGA
      121 CCGAGATAGG GTTGAGTGTT GTTCCAGTTT GGAACAAGAG TCCACTATTA AAGAACGTGG
      181 ACTCCAACGT CAAAGGGCGA AAAACCGTCT ATCAGGGCGA TGGCCCACGC TGGCTAAGAA
      241 CTCATCAGCC TCCCCGGTCC ATCTACCCAC GTACCAATGC ACCAATTGGC CACAATGACG
      301 GCTACTACAT GGTGCCATTC CTTCCTCTTT ATAGGAATGG AGACTACCTC CTGTCCAACA
      361 AGGCTCTTGG ATACGAGTAC GCCTACCTGT TGGACCCAGG TCATTGCACA ACACCAGAAA
      421 TGCCCTCTGA TCTGCAAAAG ACGTGAATAT CTGTTCAGAC ACCCATATCC ACTCTGTTCC
      481 ACACAGGTCA GAGGTTTGTC CAGGAGTTCT TGACAGAGGT GTAAAAAGTA CTCAAAAATT
      541 TTACTCAAGT GAAAGTACAA GTACTTAGGG AAAATTTTAC TCAATTAAAA GTAAAAGTAT
      601 CTGGCTAGAA TCTTACTTGA GTAAAAGTAA AAAAGTACTC CATTAAAATT GTACTTGAGT
      661 ATTAAGGAAG TAAAAGTAAA AGCAAGAAAG AAAACTAGAG ATTCTTGTTT AAGCTTTTAA
      721 TCTCAAAAAA CATTAAATGA AATGCATACA AGGTTTTATC CTGCTTTAGA ACTGTTTGTA
      781 TTTAATTATC AAACTATAAG ACAGACAATC TAATGCCAGT ACACGCTACT CAAAGTTGTA
      841 AAACCTCAGA TTTAACTTCA GTAGAAGCTG ATTCTCAAAA TTGTTAGTGT CAAGCCTAGC
      901 TCTTTTGGGG CTGAAAAGCA ATCCTGCAGT GCTGAAAAGC CTCTCACAGG CAGCCGATGC
      961 GGGAAGAGGT GTATTAGTCT TGATAGAGAG GCTGCAAATA GCAGGAAACG TGAGCAGAGA
     1021 CTCCCTGGTG TCTGAAACAC AGGCCAGATG GGCCCTCGAG CAGGAAACAG CTATGACCAT
     1081 GATTACGCCA AGCTATCAAC TTTGTATAGA AAAGTTGGGG TAATCGCTTA TCCTCGGATA
     1141 AACAATTATC CTCACGGGTA ATCGCTTATC CGCTCGGGTA ATCGCTTATC CTCGGGTAAT
     1201 CGCTTATCCT TGCAAGGGTC GACTCTAGAG GGTATATAAT GGATCCCATC GCGTCTCAGC
     1261 CTCACTTTGA GCTCCTCCAC ACGAATTCCC TCGACCTCGA AGACGCGTCA AGTTTGTACA
     1321 AAAAAGCAGG CTATATGGTG AGCAAGGGCG AGGAGCTGTT CACCGGGGTG GTGCCCATCC
     1381 TGGTCGAGCT GGACGGCGAC GTAAACGGCC ACAAGTTCAG CGTGTCCGGC GAGGGCGAGG
     1441 GCGATGCCAC CTACGGCAAG CTGACCCTGA AGTTCATCTG CACCACCGGC AAGCTGCCCG
     1501 TGCCCTGGCC CACCCTCGTG ACCACCCTGA CCTACGGCGT GCAGTGCTTC AGCCGCTACC
     1561 CCGACCACAT GAAGCAGCAC GACTTCTTCA AGTCCGCCAT GCCCGAAGGC TACGTCCAGG
     1621 AGCGCACCAT CTTCTTCAAG GACGACGGCA ACTACAAGAC CCGCGCCGAG GTGAAGTTCG
     1681 AGGGCGACAC CCTGGTGAAC CGCATCGAGC TGAAGGGCAT CGACTTCAAG GAGGACGGCA
     1741 ACATCCTGGG GCACAAGCTG GAGTACAACT ACAACAGCCA CAACGTCTAT ATCATGGCCG
     1801 ACAAGCAGAA GAACGGCATC AAGGTGAACT TCAAGATCCG CCACAACATC GAGGACGGCA
     1861 GCGTGCAGCT CGCCGACCAC TACCAGCAGA ACACCCCCAT CGGCGACGGC CCCGTGCTGC
     1921 TGCCCGACAA CCACTACCTG AGCACCCAGT CCGCCCTGAG CAAAGACCCC AACGAGAAGC
     1981 GCGATCACAT GGTCCTGCTG GAGTTCGTGA CCGCCGCCGG GATCACTCTC GGCATGGACG
     2041 AGCTGTACAA GGGCGGTGGA AGATCTGGGA ATTCAAGGCC TCTCGAGCCT CTAGATTCTG
     2101 CAGCCCTATA GTGAGTCGTA TTACGTAGAT CCAGACATGA TAAGATACAT TGATGAGTTT
     2161 GGACAAACCA CAACTAGAAT GCAGTGAAAA AAATGCTTTA TTTGTGAAAT TTGTGATGCT
     2221 ATTGCTTTAT TTGTAACCAT TATAAGCTGC AATAAACAAG TTAACAACAA CAATTGCATT
     2281 CATTTTATGT TTCAGGTTCA GGGGGAGGTG TGGGAGGTTT TTTCACCCAG CTTTCTTGTA
     2341 CAAAGTGGGG ATCATCATGA CAAAGAGAGA AGAAATCAGT TATTAGAAAT GAGTTATTAA
     2401 AACTATTATG ATTAGAAATA TGTTTCTTTC TCTTAAACAG GATTTAAGGG GAAAATATAC
     2461 AGGGAGCGAA AAATTTTGAC TTCATCTGTA TATACATATA ATAAATAATA CATCAATAAA
     2521 TTCCATCTAA ATACGCTACC ATATATATAT ATATATGTAT ATACACACAC ACACACACAC
     2581 CATCTAATAT ATGTCATGCT ACATTATTAA CTTCAGTATG AAATCTACTC TGCTCTGGAG
     2641 TGTATCAGCG AGCGACTCCT CTGCTCGGAT TATCATCACG TGACCTCTGA CCTGAACACA
     2701 GACCTCTCTT AGCCATAGGT ATACAGCCTA TAGCATAGAG CTCGGGCTGA GAGGGAAAGT
     2761 GCCGCCGTCA GGGCTTTCCA TTGACAGTAA AATGCTTACG GCCTTCTGGG CGATGCGATG
     2821 ACCCTGCGCT TCGGCACGGC TGAACTCTGA TGACGGCGTG TTTCTATTGT CCGCCGCGCG
     2881 CCGCTGTGCT GCCCTTGCCA GCGTATAAAA GCTGCGGCAG TGGTGAGGAA GGCCAGAGCG
     2941 AGCCAGCCTT CCCAGCACAG CTGTAGTTTA AACGCGTTGC CATAACTTCG TATAGGATAC
     3001 TTTATACGAA GTTATCTGCA TGCTAGGTTG CCGGGAACGG TGCATTGGAA CGCGCATTCC
     3061 CCGTGTTAAT TAACAGGTAA GTGTCTTCCT CCTGTTTCCT TCCCCTGCTA TTCTGCTCAA
     3121 CCTTCCTATC AGAAACTGCA GTATCTGTAT TTTTGCTAGG GGATCCCGCC ACCATGGTGA
     3181 GCAAGGGCGA GGAGCTGTTC ACCGGGGTGG TGCCCATCCT GGTCGAGCTG GACGGCGACG
     3241 TAAACGGCCA CAAGTTCAGC GTGTCCGGCG AGGGCGAGGG CGATGCCACC TACGGCAAGC
     3301 TGACCCTGAA GTTCATCTGC ACCACCGGCA AGCTGCCCGT GCCCTGGCCC ACCCTCGTGA
     3361 CCACCCTGAC CTGGGGCGTG CAGTGCTTCA GCCGCTACCC CGACCACATG AAGCAGCACG
     3421 ACTTCTTCAA GTCCGCCATG CCCGAAGGCT ACGTCCAGGA GCGCACCATC TTCTTCAAGG
     3481 ACGACGGCAA CTACAAGACC CGCGCCGAGG TGAAGTTCGA GGGCGACACC CTGGTGAACC
     3541 GCATCGAGCT GAAGGGCATC GACTTCAAGG AGGACGGCAA CATCCTGGGG CACAAGCTGG
     3601 AGTACAACTA CATCAGCCAC AACGTCTATA TCACCGCCGA CAAGCAGAAG AACGGCATCA
     3661 AGGCCAACTT CAAGATCCGC CACAACATCG AGGACGGCAG CGTGCAGCTC GCCGACCACT
     3721 ACCAGCAGAA CACCCCCATC GGCGACGGCC CCGTGCTGCT GCCCGACAAC CACTACCTGA
     3781 GCACCCAGTC CGCCCTGAGC AAAGACCCCA ACGAGAAGCG CGATCACATG GTCCTGCTGG
     3841 AGTTCGTGAC CGCCGCCGGG ATCACTCTCG GCATGGACGA GCTGTACAAG TAAAGCGGCC
     3901 ACCAACTTTA TTATACATAG TTGATAATTC ACTGGCCGTC GTTTTACGGT ACCATCGATG
     3961 ATGATCCAGA CATGATAAGA TACATTGATG AGTTTGGACA AACCACAACT AGAATGCAGT
     4021 GAAAAAAATG CTTTATTTGT GAAATTTGTG ATGCTATTGC TTTATTTGTA ACCATTATAA
     4081 GCTGCAATAA ACAAGTTAAC AACAACAATT GCATTCATTT TATGTTTCAG GTTCAGGGGG
     4141 AGGTGTGGGA GGTTTTTTAA AGCAAGTAAA ACCTCTACAA ATGTGGTATG GCTGATTATG
     4201 ATCCTCTAGA TCAGATCTGC GAAGATACGG CCACGGGTGC TCTTGATCCT GTGGCTGATT
     4261 TTGGACTGTG CTGCTCGCAG CTGCTGATGA ATCACATACT TCCTCCATTT TCTTCCACTG
     4321 ATTGACTGTT ATAATTTCCC TAATTTCCAG GTCAAGGTGC TGTGCATTGT GGTAATAGAT
     4381 GTGACATGAC GTCACTTCCA AAGGACCAAT GAACATGTCT GACCAATTTC ATATAATGTG
     4441 AAAACGATTT TCATAGGCAG AATAAATAAC ATTTAAATTA AACTGGGCAT CAGCGCAATT
     4501 CAATTGGTTT GGTAATAGCA AGGGAAAATA GAATGAAGTG ATCTCCAAAA AATAAGTACT
     4561 TTTTGACTGT AAATAAAATT GTAAGGAGTA AAAAGTACTT TTTTTTCTAA AAAAATGTAA
     4621 TTAAGTAAAA GTAAAAGTAT TGATTTTTAA TTGTACTCAA GTAAAGTAAA AATCCCCAAA
     4681 AATAATACTT AAGTACAGTA ATCAAGTAAA ATTACTCAAG TACTTTACAC CTCTGGTTCT
     4741 TGACCCCCTA CCTTCAGCAA GCCCAGCAGA TCCACTAGTT CTAGAGCGGC CGCCACCGCG
     4801 GTGGAGCTCC AGCTTTTGTT CCCTTTAGTG AGGGTTAATT GCGCGCTTGG CGTAATCATG
     4861 GTCATAGCTG TTTCCTGTGT GAAATTGTTA TCCGCTCACA ATTCCACACA ACATACGAGC
     4921 CGGAAGCATA AAGTGTAAAG CCTGGGGTGC CTAATGAGTG AGCTAACTCA CATTAATTGC
     4981 GTTGCGCTCA CTGCCCGCTT TCCAGTCGGG AAACCTGTCG TGCCAGCTGC ATTAATGAAT
     5041 CGGCCAACGC GCGGGGAGAG GCGGTTTGCG TATTGGGCGC TCTTCCGCTT CCTCGCTCAC
     5101 TGACTCGCTG CGCTCGGTCG TTCGGCTGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT
     5161 AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA
     5221 GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC
     5281 CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT
     5341 ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT
     5401 GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG
     5461 CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA
     5521 CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA
     5581 CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC
     5641 GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG
     5701 AAGGACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG
     5761 TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA
     5821 GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC
     5881 TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG
     5941 GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA
     6001 TGAGTAAACT TGGTCTGACA GTTACCAATG CTTAATCAGT GAGGCACCTA TCTCAGCGAT
     6061 CTGTCTATTT CGTTCATCCA TAGTTGCCTG ACTCCCCGTC GTGTAGATAA CTACGATACG
     6121 GGAGGGCTTA CCATCTGGCC CCAGTGCTGC AATGATACCG CGAGACCCAC GCTCACCGGC
     6181 TCCAGATTTA TCAGCAATAA ACCAGCCAGC CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC
     6241 AACTTTATCC GCCTCCATCC AGTCTATTAA TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC
     6301 GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC CATTGCTACA GGCATCGTGG TGTCACGCTC
     6361 GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG TTCCCAACGA TCAAGGCGAG TTACATGATC
     6421 CCCCATGTTG TGCAAAAAAG CGGTTAGCTC CTTCGGTCCT CCGATCGTTG TCAGAAGTAA
     6481 GTTGGCCGCA GTGTTATCAC TCATGGTTAT GGCAGCACTG CATAATTCTC TTACTGTCAT
     6541 GCCATCCGTA AGATGCTTTT CTGTGACTGG TGAGTACTCA ACCAAGTCAT TCTGAGAATA
     6601 GTGTATGCGG CGACCGAGTT GCTCTTGCCC GGCGTCAATA CGGGATAATA CCGCGCCACA
     6661 TAGCAGAACT TTAAAAGTGC TCATCATTGG AAAACGTTCT TCGGGGCGAA AACTCTCAAG
     6721 GATCTTACCG CTGTTGAGAT CCAGTTCGAT GTAACCCACT CGTGCACCCA ACTGATCTTC
     6781 AGCATCTTTT ACTTTCACCA GCGTTTCTGG GTGAGCAAAA ACAGGAAGGC AAAATGCCGC
     6841 AAAAAAGGGA ATAAGGGCGA CACGGAAATG TTGAATACTC ATACTCTTCC TTTTTCAATA
     6901 TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA
     6961 GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTG
//
