LOCUS       Exported               10017 bp ds-DNA     linear   SYN 08-SEP-2016
DEFINITION  plasmid CrCas KanMx optimum.
ACCESSION   .
VERSION     .
KEYWORDS    Untitled 8
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10017)
  AUTHORS   Addgene
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Sep 8, 2016 from SnapGene 3.2.1
            http://www.snapgene.com
COMMENT     SECID/File created by Clone Manager, Scientific & Educational 
            Software
            SECNOTES|Identisch mit pRS62K
FEATURES             Location/Qualifiers
     source          1..10017
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    244..1590
                     /gene="2"
                     /product="2 m DNA (partial)"
                     /note="2"
     misc_feature    1652..1680
                     /gene="amp prom"
                     /note="amp prom"
     CDS             1722..2582
                     /codon_start=1
                     /gene="AmpR"
                     /note="AmpR"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     misc_feature    2737..3356
                     /gene="pBR322"
                     /note="pBR322"
     misc_feature    3378..3646
                     /gene="SNP52p"
                     /note="SNP52p"
     misc_feature    3670..3747
                     /gene="gRNA struct"
                     /note="gRNA struct"
     misc_feature    3748..3767
                     /gene="Sup4t"
                     /note="Sup4t"
     misc_feature    complement(3788..4041)
                     /gene="Cyc1t"
                     /note="Cyc1t"
     CDS             complement(4048..8187)
                     /gene="Cas9"
                     /note="Cas9"
     misc_feature    complement(8191..8790)
                     /gene="Rox3"
                     /note="Rox3"
     misc_feature    complement(8839..9078)
                     /gene="TEFt"
                     /note="TEFt"
     CDS             complement(9079..9651)
                     /gene="NatMX"
                     /note="NatMX"
     misc_feature    complement(9652..9999)
                     /gene="pTEF"
                     /note="pTEF"
ORIGIN
        1 cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaac
       61 acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt
      121 gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag
      181 acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc
      241 ttagtatgat ccaatatcaa aggaaatgat agcattgaag gatgagacta atccaattga
      301 ggagtggcag catatagaac agctaaaggg tagtgctgaa ggaagcatac gataccccgc
      361 atggaatggg ataatatcac aggaggtact agactacctt tcatcctaca taaatagacg
      421 catataagta cgcatttaag cataaacacg cactatgccg ttcttctcat gtatatatat
      481 atacaggcaa cacgcagata taggtgcgac gtgaacagtg agctgtatgt gcgcagctcg
      541 cgttgcattt tcggaagcgc tcgttttcgg aaacgctttg aagttcctat tccgaagttc
      601 ctattctcta gaaagtatag gaacttcaga gcgcttttga aaaccaaaag cgctctgaag
      661 acgcactttc aaaaaaccaa aaacgcaccg gactgtaacg agctactaaa atattgcgaa
      721 taccgcttcc acaaacattg ctcaaaagta tctctttgct atatatctct gtgctatatc
      781 cctatataac ctacccatcc acctttcgct ccttgaactt gcatctaaac tcgacctcta
      841 cattttttat gtttatctct agtattactc tttagacaaa aaaattgtag taagaactat
      901 tcatagagtg aatcgaaaac aatacgaaaa tgtaaacatt tcctatacgt agtatataga
      961 gacaaaatag aagaaaccgt tcataatttt ctgaccaatg aagaatcatc aacgctatca
     1021 ctttctgttc acaaagtatg cgcaatccac atcggtatag aatataatcg gggatgcctt
     1081 tatcttgaaa aaatgcaccc gcagcttcgc tagtaatcag taaacgcggg aagtggagtc
     1141 aggctttttt tatggaagag aaaatagaca ccaaagtagc cttcttctaa ccttaacgga
     1201 cctacagtgc aaaaagttat caagagactg cattatagag cgcacaaagg agaaaaaaag
     1261 taatctaaga tgctttgtta gaaaaatagc gctctcggga tgcatttttg tagaacaaaa
     1321 aagaagtata gattctttgt tggtaaaata gcgctctcgc gttgcatttc tgttctgtaa
     1381 aaatgcagct cagattcttt gtttgaaaaa ttagcgctct cgcgttgcat ttttgtttta
     1441 caaaaatgaa gcacagattc ttcgttggta aaatagcgct ttcgcgttgc atttctgttc
     1501 tgtaaaaatg cagctcagat tctttgtttg aaaaattagc gctctcgcgt tgcatttttg
     1561 ttctacaaaa tgaagcacag atgcttcgtt caggtggcac ttttcgggga aatgtgcgcg
     1621 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
     1681 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
     1741 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
     1801 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
     1861 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
     1921 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag
     1981 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
     2041 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
     2101 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
     2161 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
     2221 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa
     2281 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
     2341 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
     2401 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
     2461 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
     2521 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
     2581 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
     2641 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
     2701 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     2761 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     2821 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     2881 cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact
     2941 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     3001 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     3061 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     3121 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     3181 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     3241 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     3301 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcgggct
     3361 agggataaca gggtaattct ttgaaaagat aatgtatgat tatgctttca ctcatattta
     3421 tacagaaact tgatgttttc tttcgagtat atacaaggtg attacatgta cgtttgaagt
     3481 acaactctag attttgtagt gccctcttgg gctagcggta aaggtgcgca ttttttcaca
     3541 ccctacaatg ttctgttcaa aagattttgg tcaaacgctg tagaagtgaa agttggtgcg
     3601 catgtttcgg cgttcgaaac ttctccgcag tgaaagataa atgatcggat ccacgttctc
     3661 tgcggccgct tttagagcta gaaatagcaa gttaaaataa ggctagtccg ttatcaactt
     3721 gaaaaagtgg caccgagtcg gtggtgcttt ttttgttttt tatgtctcgg cgaattgggt
     3781 accggccgca aattaaagcc ttcgagcgtc ccaaaacctt ctcaagcaag gttttcagta
     3841 taatgttaca tgcgtacacg cgtctgtaca gaaaaaaaag aaaaatttga aatataaata
     3901 acgttcttaa tactaacata actataaaaa aataaatagg gacctagact tcaggttgtc
     3961 taactccttc cttttcggtt agagcggatg tggggggagg gcgtgaatgt aagcgtgaca
     4021 taactaatta catgactcga ggtcgactta aacctttctc ttcttctttg ggtcagctct
     4081 agagtcacca cccaattgag acaagtcaat tctggtttcg tacaaaccgg taatagattg
     4141 gtgaatcaag gtagcgtcca aaacttcctt ggtagaggtg tatctctttc tgtcaatggt
     4201 ggtgtcgaag tacttgaaag cagctggagc acccaagttg gtcaaggtga acaagtgaat
     4261 aatgttttca gcttgttctc taattggctt gtctctgtgc ttgttgtaag cagacaaaac
     4321 cttgtccaag ttagcgtcag ccaaaataac tctcttagag aattcagaaa tttgttcaat
     4381 aatttcgtcc aagtagtgct tgtgttgttc aacgaacaat tgcttttgtt cgttgtcttc
     4441 tggagaaccc ttcaactttt cgtagtgaga agccaagtac aagaagttaa cgtacttaga
     4501 tggcaaagcc aattcgttac ccttttgcaa ttcaccagca gaagccaaca ttctctttct
     4561 accgttttcc aattcgaaca aagagtactt tggcaactta ataatcaagt ccttcttaac
     4621 ttccttgtaa cccttagctt ccaagaagtc aattgggttc ttttcgaaag aagatctttc
     4681 cataatggta atacccaaca attccttaac agacttcaac ttcttagact tacccttttc
     4741 aaccttagca acaaccaaaa cagagtaagc aacggttgga gagtcgaaac caccgtactt
     4801 ctttgggtcc cagtccttct ttctagcaat caacttgtca gagtttctct ttggcaaaat
     4861 agattcctta gagaaaccac cggtttgaac ttcggtcttc ttaacaatgt taacttgtgg
     4921 catagacaaa acctttctaa cggtagcgaa gtctctaccc ttgtcccaaa caatttcacc
     4981 ggtttcaccg ttggtttcaa tcaatggtct ctttctaatt tcaccgttag ccaaggtaat
     5041 ttcggtcttg aagaagttca taatgttaga gtagaagaag tacttagcgg tagccttacc
     5101 aatttcttgt tcagacttag caatcatctt tctaacgtcg taaaccttgt agtcaccgta
     5161 aacgaattca gattccaact ttgggtactt cttaatcaaa gcggtaccaa caacagcgtt
     5221 caagtaagcg tcgtgagcgt ggtggtagtt gttaatttct ctaaccttgt agaattggaa
     5281 gtcctttctg aagtcagaaa ccaacttaga cttcaaggta ataaccttaa cttctctaat
     5341 caacttgtcg ttttcgtcgt acttggtgtt cattctagag tccaaaattt gagcaacgtg
     5401 cttggtaatt tgtctggttt caaccaattg tctcttaatg aaaccagcct tgtccaattc
     5461 agacaaacca cctctttcag ccttggtcaa gttgtcgaac tttctttggg taatcaactt
     5521 agcgttcaac aattgtctcc agtagttctt catcttctta acaacttctt cagatggaac
     5581 gttgtcagac ttacctctgt tcttgtcaga tctggtcaaa accttgttgt caatagagtc
     5641 gtccttcaag aaagattgtg gaacaatgtg gtcaacgtcg tagtcagaca atctgttaat
     5701 gtccaattct tggtcaacgt acatgtctct accgttttgc aagtagtaca agtacaactt
     5761 ttcgttttgc aattgggtgt tttcaactgg gtgttccttc aaaatttgag aacccaattc
     5821 cttaatacct tcttcaattc tcttcattct ttctctagag ttcttttgac ccttttgggt
     5881 ggtttggttt tctctagcca tttcaataac aatgttttct ggcttgtgtc tacccataac
     5941 cttaaccaat tcgtcaacaa ccttaacggt ttgcaaaata cccttcttaa tagctggaga
     6001 accagccaag ttagcaatgt gttcgtgcaa agagtcacct tgaccagaaa cttgagcctt
     6061 ttgaatgtct tccttgaagg tcaaagagtc gtcgtgaatc aattgcatga agtttctgtt
     6121 agcgaaaccg tcagacttca agaagtccaa aatggtctta ccagattgct tgtctctaat
     6181 accgttaatc aactttctag acaatctacc ccaaccggtg tatcttcttc tcttcaattg
     6241 cttcataacc ttgtcgtcga acaagtgagc gtaggtcttc aatctttctt caatcatttc
     6301 tctgtcttcg aacaaggtca aggtcaaaac aatgtcttcc aaaatgtctt cgttttcttc
     6361 gttgtccaag aagtccttgt ccttaataat cttcaacaag tcgtggtagg tacccaaaga
     6421 agcgttgaat ctgtcttcaa caccagaaat ttcaacagag tcgaaacatt caatcttctt
     6481 gaagtagtct tccttcaatt gcttaacggt aacctttctg ttggtcttga acaacaagtc
     6541 aacaatagcc ttcttttgtt caccagacaa gaaagctggc tttctcatac cttcggtaac
     6601 gtacttaacc ttggtcaatt cgttgtaaac ggtgaagtat tcgtacaaca aagagtgctt
     6661 tggcaaaacc ttttcgtttg gcaagttctt gtcgaagttg gtcattcttt caatgaaaga
     6721 ttgagcagaa gcacccttgt caacaacttc ttcgaagttc catggggtaa tggtttcttc
     6781 agactttctg gtcatccaag cgaatctaga gttacctcta gccaatggac caacgtagta
     6841 tggaattctg aaggtcaaaa tcttttcaat cttttctctg ttgtccttca agaatgggta
     6901 gaagtcttct tgtcttctca aaatagcgtg caattcaccc aagtgaattt ggtgtggaat
     6961 agaaccgttg tcgaaggttc tttgctttct caacaagtct tctctgttca acttaaccaa
     7021 caattcttcg gtaccgtcca tcttttccaa aattggctta atgaacttgt agaattcttc
     7081 ttgagaagca ccaccgtcaa tgtaaccagc gtaaccgttc ttagattggt cgaagaaaat
     7141 ttccttgtac ttttctggca attgttgtct aaccaaagcc ttcaacaagg tcaagtcttg
     7201 gtggtgttcg tcgtatctct taatcataga agcagacaat ggagccttgg taatttcggt
     7261 gttaactctc aaaatgtcag acaacaaaat agcgtcagac aagttcttag cagccaagaa
     7321 caagtcagcg tattggtcac caatttgagc caacaagttg tccaagtcgt cgtcgtaggt
     7381 gtccttagac aattgcaact tagcgtcttc agccaagtcg aagttagact tgaagtttgg
     7441 ggtcaaaccc aaagacaaag caatcaagtt accgaacaaa ccgttcttct tttcacctgg
     7501 caattgagca atcaagtttt ccaatcttct agacttagac aatctagcag acaaaatagc
     7561 cttagcgtca acaccagaag cgttaattgg gttttcttcg aacaattggt tgtaggtttg
     7621 aaccaattga atgaacaact tgtcaacgtc agagttgtct gggttcaagt caccttcaat
     7681 caagaagtga cctctgaact taatcatgtg agccaaagcc aagtaaatca atctcaagtc
     7741 agccttgtcg gtagagtcaa ccaacttctt tctcaagtgg taaatggttg ggtacttttc
     7801 gtggtaagca acttcgtcaa caatgttacc gaaaattggg tgtctttcgt gcttcttgtc
     7861 ttcttcaacc aagaaagatt cttccaatct gtggaagaaa gagtcgtcaa ccttagccat
     7921 ttcgttagag aaaatttctt gcaagtaaca aattctgttc tttcttctgg tgtatcttct
     7981 tctagcggtt ctcttcaatc tggtagcttc agcggtttca ccagagtcga acaacaaagc
     8041 accaatcaag ttcttcttaa tagagtgtct gtcggtgtta cccaaaacct tgaacttctt
     8101 agatggaacc ttgtattcgt cggtaataac agcccaacca acagagttgg taccaatgtc
     8161 caaaccaata gagtacttct tgtccatatg tattatcgta ttcctttttc gttcacttac
     8221 tattgctatt cgcactcact acagatctat attttatgtt ccaccctgtt gtcagatcag
     8281 gaaaggaatg aagaggctct atttaaacca aatacacctc ttcgtttctc aatattgttg
     8341 ggctttcctt ttttttttcc ttccacgtct atctattgtc tctatcaaaa ttaggaaaaa
     8401 gaaatgaaga acgatgagcc aaacgaggct gagacaatca tgcatccgta cattctatgt
     8461 atgtttgggt gtatctttcc agattcggtt cttttttttt ttcagtccaa ttgaaaaaaa
     8521 aaaggaacag gaacaacggg tcgaaatttg ccgtcacatt cagagcgaag agcgacaggg
     8581 gaattggtgg gctaaccacc tgttgagccc agctagcagc tgtcggtaaa ccgtcgttgg
     8641 gcggggcgcg acaatgcagc cagcggaggc attgtatggt ttccgtctgg agagtgggct
     8701 tagccggcct ggcactcctt ctctaagcag agtgagaagg gaatcttttt actggagaag
     8761 agtgtttgaa tccagcagaa ggtaatacgc tgcggtattt tctccttacg catctgtgcg
     8821 gtatttcaca ccgcgttttc gacactggat ggcggcgtta gtatcgaatc gacagcagta
     8881 tagcgaccag cattcacata cgattgacgc atgatattac tttctgcgca cttaacttcg
     8941 catctgggca gatgatgtcg aggcgaaaaa aaatataaat cacgctaaca tttgattaaa
     9001 atagaacaac tacaatataa aaaaactata caaatgacaa gttcttgaaa acaagaatct
     9061 ttttattgtc agtactgatt aggggcaggg catgctcatg tagagcgcct gctcgccgtc
     9121 cgaggcggtg ccgtcgtaca gggcggtgtc caggccgcag agggtgaacc ccatccgccg
     9181 gtacgcgtgg atcgccggtg cgttgacgtt ggtgacctcc agccagaggt gcccggcgcc
     9241 ccgctcgcgg gcgaactccg tcgcgagccc catcaacgcg cgcccgaccc cgtgcccccg
     9301 gtgctccggg gcgacctcga tgtcctcgac ggtcagccgg cggttccagc cggagtacga
     9361 gacgaccacg aagcccgcca ggtcgccgtc gtccccgtac gcgacgaacg tccgggagtc
     9421 cgggtcgccg tcctccccgt cgtccgattc gtcgtccgat tcgtcgtcgg ggaacacctt
     9481 ggtcaggggc gggtccaccg gcacctcccg cagggtgaag ccgtccccgg tggcggtgac
     9541 gcggaagacg gtgtcggtgg tgaaggaccc atccagtgcc tcgatggcct cggcgtcccc
     9601 cgggacactg gtgcggtacc ggtaagccgt gtcgtcaaga gtggtaccca tggttgttta
     9661 tgttcggatg tgatgtgaga actgtatcct agcaagattt taaaaggaag tatatgaaag
     9721 aagaacctca gtggcaaatc ctaacctttt atatttctct acaggggcgc ggcgtgggga
     9781 caattcaacg cgtctgtgag gggagcgttt ccctgctcgc aggtctgcag cgaggagccg
     9841 taatttttgc ttcgcgccgt gcggccatca aaatgtatgg atgcaaatga ttatacatgg
     9901 ggatgtatgg gctaaatgta cgggcgacag tcacatcatg cccctgagct gcgcacgtca
     9961 agactgtcaa ggagggtatt ctgggcctcc atgtcgctga ttaccctgtt atcccta
//
