LOCUS       Exported               10560 bp ds-DNA     circular SYN 23-SEP-2016
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    81193
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10560)
  AUTHORS   Addgene
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Sep 23, 2016 from SnapGene 3.2.1
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10560
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        53..321
                     /note="SNR52 promoter"
                     /note="promoter for the S. cerevisiae small nucleolar RNA 
                     gene SNR52"
     misc_RNA        345..418
                     /note="gRNA scaffold"
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     terminator      423..442
                     /note="SUP4 terminator"
                     /note="transcription terminator for the S. cerevisiae SUP4 
                     tRNA gene"
     rep_origin      554..1896
                     /note="2u ori"
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    886..933
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FRT"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     promoter        1923..2027
                     /gene="bla"
                     /note="AmpR promoter"
     CDS             2028..2888
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      3059..3647
                     /direction=RIGHT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    3666..3683
                     /note="I-SceI"
     promoter        3684..3952
                     /note="SNR52 promoter"
                     /note="promoter for the S. cerevisiae small nucleolar RNA 
                     gene SNR52"
     misc_RNA        3976..4049
                     /note="gRNA scaffold"
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     terminator      4054..4073
                     /note="SUP4 terminator"
                     /note="transcription terminator for the S. cerevisiae SUP4 
                     tRNA gene"
     terminator      4094..4341
                     /gene="S. cerevisiae CYC1"
                     /note="CYC1 terminator"
                     /note="transcription terminator for CYC1"
     CDS             complement(4357..4377)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /note="SV40 NLS"
                     /translation="PKKKRKV"
     CDS             complement(4390..8493)
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /note="Cas9"
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     gene            complement(9182..10538)
                     /note="kanMX"
                     /note="yeast selectable marker conferring kanamycin 
                     resistance (Wach et al., 1994)"
     terminator      9182..9379
                     /note="TEF terminator"
                     /note="Ashbya gossypii TEF terminator"
     CDS             complement(9385..10194)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /note="KanR"
                     /note="confers resistance to kanamycin"
                     /translation="MGKEKTHVSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     promoter        complement(10195..10538)
                     /note="TEF promoter"
                     /note="Ashbya gossypii TEF promoter"
     misc_feature    10543..10560
                     /note="I-SceI"
ORIGIN
        1 cactctcagt acaatctgct ctgatgccgc atagttaagc cagccgggta attctttgaa
       61 aagataatgt atgattatgc tttcactcat atttatacag aaacttgatg ttttctttcg
      121 agtatataca aggtgattac atgtacgttt gaagtacaac tctagatttt gtagtgccct
      181 cttgggctag cggtaaaggt gcgcattttt tcacacccta caatgttctg ttcaaaagat
      241 tttggtcaaa cgctgtagaa gtgaaagttg gtgcgcatgt ttcggcgttc gaaacttctc
      301 cgcagtgaaa gataaatgat cggatccacg ttctctgcgg ccgcttttag agctagaaat
      361 agcaagttaa aataaggcta gtccgttatc aacttgaaaa agtggcaccg agtcggtggt
      421 gctttttttg ttttttatgt ctcggcgaat tgggtgcggc cgcgtttgtc acctttcata
      481 ggcctgacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat
      541 ggtttcttag tatgatccaa tatcaaagga aatgatagca ttgaaggatg agactaatcc
      601 aattgaggag tggcagcata tagaacagct aaagggtagt gctgaaggaa gcatacgata
      661 ccccgcatgg aatgggataa tatcacagga ggtactagac tacctttcat cctacataaa
      721 tagacgcata taagtacgca tttaagcata aacacgcact atgccgttct tctcatgtat
      781 atatatatac aggcaacacg cagatatagg tgcgacgtga acagtgagct gtatgtgcgc
      841 agctcgcgtt gcattttcgg aagcgctcgt tttcggaaac gctttgaagt tcctattccg
      901 aagttcctat tctctagaaa gtataggaac ttcagagcgc ttttgaaaac caaaagcgct
      961 ctgaagacgc actttcaaaa aaccaaaaac gcaccggact gtaacgagct actaaaatat
     1021 tgcgaatacc gcttccacaa acattgctca aaagtatctc tttgctatat atctctgtgc
     1081 tatatcccta tataacctac ccatccacct ttcgctcctt gaacttgcat ctaaactcga
     1141 cctctacatt ttttatgttt atctctagta ttactcttta gacaaaaaaa ttgtagtaag
     1201 aactattcat agagtgaatc gaaaacaata cgaaaatgta aacatttcct atacgtagta
     1261 tatagagaca aaatagaaga aaccgttcat aattttctga ccaatgaaga atcatcaacg
     1321 ctatcacttt ctgttcacaa agtatgcgca atccacatcg gtatagaata taatcgggga
     1381 tgcctttatc ttgaaaaaat gcacccgcag cttcgctagt aatcagtaaa cgcgggaagt
     1441 ggagtcaggc tttttttatg gaagagaaaa tagacaccaa agtagccttc ttctaacctt
     1501 aacggaccta cagtgcaaaa agttatcaag agactgcatt atagagcgca caaaggagaa
     1561 aaaaagtaat ctaagatgct ttgttagaaa aatagcgctc tcgggatgca tttttgtaga
     1621 acaaaaaaga agtatagatt ctttgttggt aaaatagcgc tctcgcgttg catttctgtt
     1681 ctgtaaaaat gcagctcaga ttctttgttt gaaaaattag cgctctcgcg ttgcattttt
     1741 gttttacaaa aatgaagcac agattcttcg ttggtaaaat agcgctttcg cgttgcattt
     1801 ctgttctgta aaaatgcagc tcagattctt tgtttgaaaa attagcgctc tcgcgttgca
     1861 tttttgttct acaaaatgaa gcacagatgc ttcgttcagg tggcactttt cggggaaatg
     1921 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     1981 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
     2041 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     2101 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
     2161 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
     2221 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
     2281 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
     2341 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
     2401 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
     2461 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
     2521 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     2581 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     2641 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     2701 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     2761 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     2821 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     2881 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     2941 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     3001 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     3061 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     3121 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     3181 gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca
     3241 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     3301 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     3361 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     3421 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     3481 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     3541 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     3601 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     3661 cgggctaggg ataacagggt aattctttga aaagataatg tatgattatg ctttcactca
     3721 tatttataca gaaacttgat gttttctttc gagtatatac aaggtgatta catgtacgtt
     3781 tgaagtacaa ctctagattt tgtagtgccc tcttgggcta gcggtaaagg tgcgcatttt
     3841 ttcacaccct acaatgttct gttcaaaaga ttttggtcaa acgctgtaga agtgaaagtt
     3901 ggtgcgcatg tttcggcgtt cgaaacttct ccgcagtgaa agataaatga tcggatccac
     3961 gttctctgcg gccgctttta gagctagaaa tagcaagtta aaataaggct agtccgttat
     4021 caacttgaaa aagtggcacc gagtcggtgg tgcttttttt gttttttatg tctcggcgaa
     4081 ttgggtaccg gccgcaaatt aaagccttcg agcgtcccaa aaccttctca agcaaggttt
     4141 tcagtataat gttacatgcg tacacgcgtc tgtacagaaa aaaaagaaaa atttgaaata
     4201 taaataacgt tcttaatact aacataacta taaaaaaata aatagggacc tagacttcag
     4261 gttgtctaac tccttccttt tcggttagag cggatgtggg gggagggcgt gaatgtaagc
     4321 gtgacataac taattacatg actcgaggtc gacttaaacc tttctcttct tctttgggtc
     4381 agctctagag tcaccaccca attgagacaa gtcaattctg gtttcgtaca aaccggtaat
     4441 agattggtga atcaaggtag cgtccaaaac ttccttggta gaggtgtatc tctttctgtc
     4501 aatggtggtg tcgaagtact tgaaagcagc tggagcaccc aagttggtca aggtgaacaa
     4561 gtgaataatg ttttcagctt gttctctaat tggcttgtct ctgtgcttgt tgtaagcaga
     4621 caaaaccttg tccaagttag cgtcagccaa aataactctc ttagagaatt cagaaatttg
     4681 ttcaataatt tcgtccaagt agtgcttgtg ttgttcaacg aacaattgct tttgttcgtt
     4741 gtcttctgga gaacccttca acttttcgta gtgagaagcc aagtacaaga agttaacgta
     4801 cttagatggc aaagccaatt cgttaccctt ttgcaattca ccagcagaag ccaacattct
     4861 ctttctaccg ttttccaatt cgaacaaaga gtactttggc aacttaataa tcaagtcctt
     4921 cttaacttcc ttgtaaccct tagcttccaa gaagtcaatt gggttctttt cgaaagaaga
     4981 tctttccata atggtaatac ccaacaattc cttaacagac ttcaacttct tagacttacc
     5041 cttttcaacc ttagcaacaa ccaaaacaga gtaagcaacg gttggagagt cgaaaccacc
     5101 gtacttcttt gggtcccagt ccttctttct agcaatcaac ttgtcagagt ttctctttgg
     5161 caaaatagat tccttagaga aaccaccggt ttgaacttcg gtcttcttaa caatgttaac
     5221 ttgtggcata gacaaaacct ttctaacggt agcgaagtct ctacccttgt cccaaacaat
     5281 ttcaccggtt tcaccgttgg tttcaatcaa tggtctcttt ctaatttcac cgttagccaa
     5341 ggtaatttcg gtcttgaaga agttcataat gttagagtag aagaagtact tagcggtagc
     5401 cttaccaatt tcttgttcag acttagcaat catctttcta acgtcgtaaa ccttgtagtc
     5461 accgtaaacg aattcagatt ccaactttgg gtacttctta atcaaagcgg taccaacaac
     5521 agcgttcaag taagcgtcgt gagcgtggtg gtagttgtta atttctctaa ccttgtagaa
     5581 ttggaagtcc tttctgaagt cagaaaccaa cttagacttc aaggtaataa ccttaacttc
     5641 tctaatcaac ttgtcgtttt cgtcgtactt ggtgttcatt ctagagtcca aaatttgagc
     5701 aacgtgcttg gtaatttgtc tggtttcaac caattgtctc ttaatgaaac cagccttgtc
     5761 caattcagac aaaccacctc tttcagcctt ggtcaagttg tcgaactttc tttgggtaat
     5821 caacttagcg ttcaacaatt gtctccagta gttcttcatc ttcttaacaa cttcttcaga
     5881 tggaacgttg tcagacttac ctctgttctt gtcagatctg gtcaaaacct tgttgtcaat
     5941 agagtcgtcc ttcaagaaag attgtggaac aatgtggtca acgtcgtagt cagacaatct
     6001 gttaatgtcc aattcttggt caacgtacat gtctctaccg ttttgcaagt agtacaagta
     6061 caacttttcg ttttgcaatt gggtgttttc aactgggtgt tccttcaaaa tttgagaacc
     6121 caattcctta ataccttctt caattctctt cattctttct ctagagttct tttgaccctt
     6181 ttgggtggtt tggttttctc tagccatttc aataacaatg ttttctggct tgtgtctacc
     6241 cataacctta accaattcgt caacaacctt aacggtttgc aaaataccct tcttaatagc
     6301 tggagaacca gccaagttag caatgtgttc gtgcaaagag tcaccttgac cagaaacttg
     6361 agccttttga atgtcttcct tgaaggtcaa agagtcgtcg tgaatcaatt gcatgaagtt
     6421 tctgttagcg aaaccgtcag acttcaagaa gtccaaaatg gtcttaccag attgcttgtc
     6481 tctaataccg ttaatcaact ttctagacaa tctaccccaa ccggtgtatc ttcttctctt
     6541 caattgcttc ataaccttgt cgtcgaacaa gtgagcgtag gtcttcaatc tttcttcaat
     6601 catttctctg tcttcgaaca aggtcaaggt caaaacaatg tcttccaaaa tgtcttcgtt
     6661 ttcttcgttg tccaagaagt ccttgtcctt aataatcttc aacaagtcgt ggtaggtacc
     6721 caaagaagcg ttgaatctgt cttcaacacc agaaatttca acagagtcga aacattcaat
     6781 cttcttgaag tagtcttcct tcaattgctt aacggtaacc tttctgttgg tcttgaacaa
     6841 caagtcaaca atagccttct tttgttcacc agacaagaaa gctggctttc tcataccttc
     6901 ggtaacgtac ttaaccttgg tcaattcgtt gtaaacggtg aagtattcgt acaacaaaga
     6961 gtgctttggc aaaacctttt cgtttggcaa gttcttgtcg aagttggtca ttctttcaat
     7021 gaaagattga gcagaagcac ccttgtcaac aacttcttcg aagttccatg gggtaatggt
     7081 ttcttcagac tttctggtca tccaagcgaa tctagagtta cctctagcca atggaccaac
     7141 gtagtatgga attctgaagg tcaaaatctt ttcaatcttt tctctgttgt ccttcaagaa
     7201 tgggtagaag tcttcttgtc ttctcaaaat agcgtgcaat tcacccaagt gaatttggtg
     7261 tggaatagaa ccgttgtcga aggttctttg ctttctcaac aagtcttctc tgttcaactt
     7321 aaccaacaat tcttcggtac cgtccatctt ttccaaaatt ggcttaatga acttgtagaa
     7381 ttcttcttga gaagcaccac cgtcaatgta accagcgtaa ccgttcttag attggtcgaa
     7441 gaaaatttcc ttgtactttt ctggcaattg ttgtctaacc aaagccttca acaaggtcaa
     7501 gtcttggtgg tgttcgtcgt atctcttaat catagaagca gacaatggag ccttggtaat
     7561 ttcggtgtta actctcaaaa tgtcagacaa caaaatagcg tcagacaagt tcttagcagc
     7621 caagaacaag tcagcgtatt ggtcaccaat ttgagccaac aagttgtcca agtcgtcgtc
     7681 gtaggtgtcc ttagacaatt gcaacttagc gtcttcagcc aagtcgaagt tagacttgaa
     7741 gtttggggtc aaacccaaag acaaagcaat caagttaccg aacaaaccgt tcttcttttc
     7801 acctggcaat tgagcaatca agttttccaa tcttctagac ttagacaatc tagcagacaa
     7861 aatagcctta gcgtcaacac cagaagcgtt aattgggttt tcttcgaaca attggttgta
     7921 ggtttgaacc aattgaatga acaacttgtc aacgtcagag ttgtctgggt tcaagtcacc
     7981 ttcaatcaag aagtgacctc tgaacttaat catgtgagcc aaagccaagt aaatcaatct
     8041 caagtcagcc ttgtcggtag agtcaaccaa cttctttctc aagtggtaaa tggttgggta
     8101 cttttcgtgg taagcaactt cgtcaacaat gttaccgaaa attgggtgtc tttcgtgctt
     8161 cttgtcttct tcaaccaaga aagattcttc caatctgtgg aagaaagagt cgtcaacctt
     8221 agccatttcg ttagagaaaa tttcttgcaa gtaacaaatt ctgttctttc ttctggtgta
     8281 tcttcttcta gcggttctct tcaatctggt agcttcagcg gtttcaccag agtcgaacaa
     8341 caaagcacca atcaagttct tcttaataga gtgtctgtcg gtgttaccca aaaccttgaa
     8401 cttcttagat ggaaccttgt attcgtcggt aataacagcc caaccaacag agttggtacc
     8461 aatgtccaaa ccaatagagt acttcttgtc catatgtatt atcgtattcc tttttcgttc
     8521 acttactatt gctattcgca ctcactacag atctatattt tatgttccac cctgttgtca
     8581 gatcaggaaa ggaatgaaga ggctctattt aaaccaaata cacctcttcg tttctcaata
     8641 ttgttgggct ttcctttttt ttttccttcc acgtctatct attgtctcta tcaaaattag
     8701 gaaaaagaaa tgaagaacga tgagccaaac gaggctgaga caatcatgca tccgtacatt
     8761 ctatgtatgt ttgggtgtat ctttccagat tcggttcttt ttttttttca gtccaattga
     8821 aaaaaaaaag gaacaggaac aacgggtcga aatttgccgt cacattcaga gcgaagagcg
     8881 acaggggaat tggtgggcta accacctgtt gagcccagct agcagctgtc ggtaaaccgt
     8941 cgttgggcgg ggcgcgacaa tgcagccagc ggaggcattg tatggtttcc gtctggagag
     9001 tgggcttagc cggcctggca ctccttctct aagcagagtg agaagggaat ctttttactg
     9061 gagaagagtg tttgaatcca gcagaaggta atacgctgcg gtattttctc cttacgcatc
     9121 tgtgcggtat ttcacaccgc gttttcgaca ctggatggcg gcgttagtat cgaatcgaca
     9181 gcagtatagc gaccagcatt cacatacgat tgacgcatga tattactttc tgcgcactta
     9241 acttcgcatc tgggcagatg atgtcgaggc gaaaaaaaat ataaatcacg ctaacatttg
     9301 attaaaatag aacaactaca atataaaaaa actatacaaa tgacaagttc ttgaaaacaa
     9361 gaatcttttt attgtcagta ctgattagaa aaactcatcg agcatcaaat gaaactgcaa
     9421 tttattcata tcaggattat caataccata tttttgaaaa agccgtttct gtaatgaagg
     9481 agaaaactca ccgaggcagt tccataggat ggcaagatcc tggtatcggt ctgcgattcc
     9541 gactcgtcca acatcaatac aacctattaa tttcccctcg tcaaaaataa ggttatcaag
     9601 tgagaaatca ccatgagtga cgactgaatc cggtgagaat ggcaaaagct tatgcatttc
     9661 tttccagact tgttcaacag gccagccatt acgctcgtca tcaaaatcac tcgcatcaac
     9721 caaaccgtta ttcattcgtg attgcgcctg agcgagacga aatacgcgat cgctgttaaa
     9781 aggacaatta caaacaggaa tcgaatgcaa ccggcgcagg aacactgcca gcgcatcaac
     9841 aatattttca cctgaatcag gatattcttc taatacctgg aatgctgttt tgccggggat
     9901 cgcagtggtg agtaaccatg catcatcagg agtacggata aaatgcttga tggtcggaag
     9961 aggcataaat tccgtcagcc agtttagtct gaccatctca tctgtaacat cattggcaac
    10021 gctacctttg ccatgtttca gaaacaactc tggcgcatcg ggcttcccat acaatcgata
    10081 gattgtcgca cctgattgcc cgacattatc gcgagcccat ttatacccat ataaatcagc
    10141 atccatgttg gaatttaatc gcggcctcga aacgtgagtc ttttccttac ccatggttgt
    10201 ttatgttcgg atgtgatgtg agaactgtat cctagcaaga ttttaaaagg aagtatatga
    10261 aagaagaacc tcagtggcaa atcctaacct tttatatttc tctacagggg cgcggcgtgg
    10321 ggacaattca acgcgtctgt gaggggagcg tttccctgct cgcaggtctg cagcgaggag
    10381 ccgtaatttt tgcttcgcgc cgtgcggcca tcaaaatgta tggatgcaaa tgattataca
    10441 tggggatgta tgggctaaat gtacgggcga cagtcacatc atgcccctga gctgcgcacg
    10501 tcaagactgt caaggagggt attctgggcc tccatgtcgc tgattaccct gttatcccta
//
