LOCUS       BC132(pHU6.Ch10-rev-2)- 2263 bp ds-DNA     circular     30-AUG-2016
DEFINITION  .
ACCESSION   urn.local...ar-6f8l5qm                                             
VERSION     urn.local...ar-6f8l5qm                                             
FEATURES             Location/Qualifiers
     misc_feature    1316..1336
                     /label="Ch10-rev-2"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    1421..1421
                     /label="AGCTTGGGCCG"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     promoter        complement(903..931)
                     /label="amp prom"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    1317..1336
                     /label="AGACGATTAATGCGTCTC"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     promoter        1052..1308
                     /label="hU6 prom"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    complement(1..861)
                     /label="amp marker"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     primer_bind     1337..1419
                     /label="Spy gRNA hairpins (constant)"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    1420..1420
                     /label=""
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    complement(1490..2109)
                     /label="pBR322 origin"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
ORIGIN
        1 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
       61 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
      121 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
      181 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
      241 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
      301 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
      361 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
      421 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
      481 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
      541 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
      601 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
      661 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
      721 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
      781 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
      841 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
      901 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
      961 tccgcgcaca tttccccgaa aagtgccacc tgacgtcgct agctgtacaa aaaagcaggc
     1021 tttaaaggaa ccaattcagt cgactggatc caaggtcggg caggaagagg gcctatttcc
     1081 catgattcct tcatatttgc atatacgata caaggctgtt agagagataa ttagaattaa
     1141 tttgactgta aacacaaaga tattagtaca aaatacgtga cgtagaaagt aataatttct
     1201 tgggtagttt gcagttttaa aattatgttt taaaatggac tatcatatgc ttaccgtaac
     1261 ttgaaagtat ttcgatttct tggctttata tatcttgtgg aaaggacgaa acaccgcctc
     1321 agggcctgtg atgggagttt tagagctaga aatagcaagt taaaataagg ctagtccgtt
     1381 atcaacttga aaaagtggca ccgagtcggt gctttttttt ctcgaggtac ctctctacat
     1441 atgacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     1501 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     1561 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     1621 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     1681 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     1741 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     1801 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     1861 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     1921 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
     1981 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     2041 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     2101 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     2161 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     2221 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cag
//