LOCUS       BC135(pHU6.Ch10-for-1)- 2262 bp ds-DNA     circular     30-AUG-2016
DEFINITION  .
ACCESSION   urn.local...1v5-5r2m52v                                            
VERSION     urn.local...1v5-5r2m52v                                            
FEATURES             Location/Qualifiers
     misc_feature    complement(1389..2008)
                     /label="pBR322 origin"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    1217..1235
                     /label="AGACGATTAATGCGTCTC"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    complement(2163..761)
                     /label="amp marker"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    1319..1319
                     /label=""
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    1216..1235
                     /label="Ch10-for-1"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     promoter        952..1208
                     /label="hU6 prom"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    1320..1320
                     /label="AGCTTGGGCCG"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     primer_bind     1236..1318
                     /label="Spy gRNA hairpins (constant)"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     promoter        complement(803..831)
                     /label="amp prom"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
ORIGIN
        1 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
       61 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
      121 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
      181 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
      241 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
      301 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
      361 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
      421 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
      481 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
      541 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
      601 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
      661 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
      721 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
      781 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
      841 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtcgct
      901 agctgtacaa aaaagcaggc tttaaaggaa ccaattcagt cgactggatc caaggtcggg
      961 caggaagagg gcctatttcc catgattcct tcatatttgc atatacgata caaggctgtt
     1021 agagagataa ttagaattaa tttgactgta aacacaaaga tattagtaca aaatacgtga
     1081 cgtagaaagt aataatttct tgggtagttt gcagttttaa aattatgttt taaaatggac
     1141 tatcatatgc ttaccgtaac ttgaaagtat ttcgatttct tggctttata tatcttgtgg
     1201 aaaggacgaa acaccgccca tgacccttct cctctgtttt agagctagaa atagcaagtt
     1261 aaaataaggc tagtccgtta tcaacttgaa aaagtggcac cgagtcggtg cttttttttc
     1321 tcgaggtacc tctctacata tgacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     1381 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     1441 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     1501 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     1561 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     1621 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     1681 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     1741 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     1801 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
     1861 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     1921 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     1981 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     2041 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     2101 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     2161 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     2221 atagttgcct gactccccgt cgtgtagata actacgatac gg
//