LOCUS       BC155-(pHU6.Ch5_1551830 2190 bp ds-DNA     circular     30-AUG-2016
DEFINITION  .
ACCESSION   urn.local...1v6-5r2m52w                                            
VERSION     urn.local...1v6-5r2m52w                                            
FEATURES             Location/Qualifiers
     misc_feature    complement(1606..1637)
                     /label="JB0447"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    complement(1213..1237)
                     /label="JB0453"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    942..942
                     /label="AGCTTGGGCCG"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     primer_bind     1468..1492
                     /label="JB0744"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     promoter        complement(425..453)
                     /label="amp prom"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    839..857
                     /label="AGACGATTAATGCGTCTC"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer_bind     398..432
                     /label="JB0944"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    1787..378
                     /label="ACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATA"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     primer_bind     complement(816..837)
                     /label="JB1185"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    838..857
                     /label="Ch5_155183064-gRNA-for"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    complement(1876..1900)
                     /label="JB0392"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    complement(13..39)
                     /label="JB0761"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer_bind     1213..1237
                     /label="JB0454"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     source          complement(1785..383)
                     /label="source"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     promoter        574..830
                     /label="hU6 prom"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    complement(1587..1613)
                     /label="JB0767"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     mat_peptide     complement(1785..383)
                     /label="aadA (Spec Resistance)"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    941..941
                     /label=""
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     primer_bind     complement(178..197)
                     /label="NMG-18"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     primer_bind     858..877
                     /label="annealing region"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     primer_bind     13..39
                     /label="JB0760"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    complement(1011..1630)
                     /label="pBR322 origin"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    complement(920..940)
                     /label="JB0295"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    858..940
                     /label="Spy gRNA hairpins (constant)"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
ORIGIN
        1 tcgatcgtgg ctggctcgaa gatacctgca agaatgtcat tgcgctgcca ttctccaaat
       61 tgcagttcgc gcttagctgg ataacgccac ggaatgatgt cgtcgtgcac aacaatggtg
      121 acttctacag cgcggagaat ctcgctctct ccaggggaag ccgaagtttc caaaaggtcg
      181 ttgatcaaag ctcgccgcgt tgtttcatca agccttacgg tcaccgtaac cagcaaatca
      241 atatcactgt gtggcttcag gccgccatcc actgcggagc cgtacaaatg tacggccagc
      301 aacgtcggtt cgagatggcg ctcgatgacg ccaactacct ctgatagttg agtcgatact
      361 tcggcgatca ccgcttccct catactcttc ctttttcaat attattgaag catttatcag
      421 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaatatgg
      481 gttccgcgca catttccccg aaaagtgcca cctgacgtcg ctagctgtac aaaaaagcag
      541 gctttaaagg aaccaattca ttcgactgga tccaaggtcg ggcaggaaga gggcctattt
      601 cccatgattc cttcatattt gcatatacga tacaaggctg ttagagagat aattagaatt
      661 aatttgactg taaacacaaa gatattagta caaaatacgt gacgtagaaa gtaataattt
      721 cttgggtagt ttgcagtttt aaaattatgt tttaaaatgg actatcatat gcttaccgta
      781 acttgaaagt atttcgattt cttggcttta tatatcttgt ggaaaggacg aaacaccgga
      841 actcatgtga ttaactggtt ttagagctag aaatagcaag ttaaaataag gctagtccgt
      901 tatcaacttg aaaaagtggc accgagtcgg tgcttttttt tctcgaggta cctctctaca
      961 tatgacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     1021 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     1081 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     1141 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     1201 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     1261 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     1321 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     1381 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     1441 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
     1501 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     1561 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     1621 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     1681 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     1741 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagtttgcc gactaccttg
     1801 gtgatctcgc ctttcacgta gtggacaaat tcttccaact gatctgcgcg cgaggccaag
     1861 cgatcttctt cttgtccaag ataagcctgt ctagcttcaa gtatgacggg ctgatactgg
     1921 gccggcaggc gctccattgc ccagtcggca gcgacatcct tcggcgcgat tttgccggtt
     1981 actgcgctgt accaaatgcg ggacaacgta agcactacat ttcgctcatc gccagcccag
     2041 tcgggcggcg agttccatag cgttaaggtt tcatttagcg cctcaaatag atcctgttca
     2101 ggaaccggat caaagagttc ctccgccgct ggacctacca aggcaacgct atgttctctt
     2161 gcttttgtca gcaagatagc cagatcaatg
//