LOCUS BC156-(pHU6.Ch5_1551830 2191 bp ds-DNA circular 30-AUG-2016 DEFINITION . ACCESSION urn.local...1vf-5r2m52z VERSION urn.local...1vf-5r2m52z FEATURES Location/Qualifiers promoter 699..955 /label="hU6 prom" /ApEinfo_revcolor=#b7e6d7 /ApEinfo_fwdcolor=#b7e6d7 misc_feature complement(1137..1756) /label="pBR322 origin" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 primer_bind 984..1066 /label="Spy gRNA hairpins (constant)" /ApEinfo_revcolor=#d6b295 /ApEinfo_fwdcolor=#d6b295 misc_feature 1067..1067 /label="" /ApEinfo_revcolor=#faac61 /ApEinfo_fwdcolor=#faac61 misc_feature 963..983 /label="Ch5_155183064-gRNA-rev-1" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e misc_feature 1913..503 /label="ACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATA" /ApEinfo_revcolor=#84b0dc /ApEinfo_fwdcolor=#84b0dc mat_peptide complement(1911..508) /label="aadA (Spec Resistance)" /ApEinfo_revcolor=#b7e6d7 /ApEinfo_fwdcolor=#b7e6d7 misc_feature 964..983 /label="AGACGATTAATGCGTCTC" /ApEinfo_revcolor=#b1ff67 /ApEinfo_fwdcolor=#b1ff67 misc_feature 1068..1068 /label="AGCTTGGGCCG" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e promoter complement(550..578) /label="amp prom" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 source complement(1911..508) /label="source" /ApEinfo_revcolor=#d59687 /ApEinfo_fwdcolor=#d59687 primer_bind complement(303..322) /label="NMG-18" /ApEinfo_revcolor=#f8d3a9 /ApEinfo_fwdcolor=#f8d3a9 ORIGIN 1 aggtttcatt tagcgcctca aatagatcct gttcaggaac cggatcaaag agttcctccg 61 ccgctggacc taccaaggca acgctatgtt ctcttgcttt tgtcagcaag atagccagat 121 caatgtcgat cgtggctggc tcgaagatac ctgcaagaat gtcattgcgc tgccattctc 181 caaattgcag ttcgcgctta gctggataac gccacggaat gatgtcgtcg tgcacaacaa 241 tggtgacttc tacagcgcgg agaatctcgc tctctccagg ggaagccgaa gtttccaaaa 301 ggtcgttgat caaagctcgc cgcgttgttt catcaagcct tacggtcacc gtaaccagca 361 aatcaatatc actgtgtggc ttcaggccgc catccactgc ggagccgtac aaatgtacgg 421 ccagcaacgt cggttcgaga tggcgctcga tgacgccaac tacctctgat agttgagtcg 481 atacttcggc gatcaccgct tccctcatac tcttcctttt tcaatattat tgaagcattt 541 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa 601 tatgggttcc gcgcacattt ccccgaaaag tgccacctga cgtcgctagc tgtacaaaaa 661 agcaggcttt aaaggaacca attcattcga ctggatccaa ggtcgggcag gaagagggcc 721 tatttcccat gattccttca tatttgcata tacgatacaa ggctgttaga gagataatta 781 gaattaattt gactgtaaac acaaagatat tagtacaaaa tacgtgacgt agaaagtaat 841 aatttcttgg gtagtttgca gttttaaaat tatgttttaa aatggactat catatgctta 901 ccgtaacttg aaagtatttc gatttcttgg ctttatatat cttgtggaaa ggacgaaaca 961 ccgtctacct ctcatgagcc ggtgttttag agctagaaat agcaagttaa aataaggcta 1021 gtccgttatc aacttgaaaa agtggcaccg agtcggtgct tttttttctc gaggtacctc 1081 tctacatatg acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg 1141 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca 1201 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc 1261 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc 1321 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag 1381 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc 1441 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca 1501 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg 1561 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg 1621 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct 1681 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa 1741 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa 1801 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa 1861 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag tttgccgact 1921 accttggtga tctcgccttt cacgtagtgg acaaattctt ccaactgatc tgcgcgcgag 1981 gccaagcgat cttcttcttg tccaagataa gcctgtctag cttcaagtat gacgggctga 2041 tactgggccg gcaggcgctc cattgcccag tcggcagcga catccttcgg cgcgattttg 2101 ccggttactg cgctgtacca aatgcgggac aacgtaagca ctacatttcg ctcatcgcca 2161 gcccagtcgg gcggcgagtt ccatagcgtt a //