LOCUS       BC165-(pHU6.Ch13_102010 2190 bp ds-DNA     circular     30-AUG-2016
DEFINITION  .
ACCESSION   urn.local...1v8-5r2m52w                                            
VERSION     urn.local...1v8-5r2m52w                                            
FEATURES             Location/Qualifiers
     misc_feature    1329..1329
                     /label="AGCTTGGGCCG"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    complement(1398..2017)
                     /label="pBR322 origin"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    1226..1243
                     /label="AGACGATTAATGCGTCT"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     promoter        961..1217
                     /label="hU6 prom"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    1328..1328
                     /label=""
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    2174..765
                     /label="ACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATA"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     mat_peptide     complement(2172..770)
                     /label="aadA (Spec Resistance)"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     promoter        complement(812..840)
                     /label="amp prom"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     primer_bind     complement(565..584)
                     /label="NMG-18"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    1225..1244
                     /label="Ch13_102010574-gRNA-for"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     primer_bind     1245..1327
                     /label="Spy gRNA hairpins (constant)"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     source          complement(2172..770)
                     /label="source"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
ORIGIN
        1 atctcgcctt tcacgtagtg gacaaattct tccaactgat ctgcgcgcga ggccaagcga
       61 tcttcttctt gtccaagata agcctgtcta gcttcaagta tgacgggctg atactgggcc
      121 ggcaggcgct ccattgccca gtcggcagcg acatccttcg gcgcgatttt gccggttact
      181 gcgctgtacc aaatgcggga caacgtaagc actacatttc gctcatcgcc agcccagtcg
      241 ggcggcgagt tccatagcgt taaggtttca tttagcgcct caaatagatc ctgttcagga
      301 accggatcaa agagttcctc cgccgctgga cctaccaagg caacgctatg ttctcttgct
      361 tttgtcagca agatagccag atcaatgtcg atcgtggctg gctcgaagat acctgcaaga
      421 atgtcattgc gctgccattc tccaaattgc agttcgcgct tagctggata acgccacgga
      481 atgatgtcgt cgtgcacaac aatggtgact tctacagcgc ggagaatctc gctctctcca
      541 ggggaagccg aagtttccaa aaggtcgttg atcaaagctc gccgcgttgt ttcatcaagc
      601 cttacggtca ccgtaaccag caaatcaata tcactgtgtg gcttcaggcc gccatccact
      661 gcggagccgt acaaatgtac ggccagcaac gtcggttcga gatggcgctc gatgacgcca
      721 actacctctg atagttgagt cgatacttcg gcgatcaccg cttccctcat actcttcctt
      781 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
      841 tgtatttaga aaaataaaca aatatgggtt ccgcgcacat ttccccgaaa agtgccacct
      901 gacgtcgcta gctgtacaaa aaagcaggct ttaaaggaac caattcattc gactggatcc
      961 aaggtcgggc aggaagaggg cctatttccc atgattcctt catatttgca tatacgatac
     1021 aaggctgtta gagagataat tagaattaat ttgactgtaa acacaaagat attagtacaa
     1081 aatacgtgac gtagaaagta ataatttctt gggtagtttg cagttttaaa attatgtttt
     1141 aaaatggact atcatatgct taccgtaact tgaaagtatt tcgatttctt ggctttatat
     1201 atcttgtgga aaggacgaaa caccggagac ttaagtccaa aaccgtttta gagctagaaa
     1261 tagcaagtta aaataaggct agtccgttat caacttgaaa aagtggcacc gagtcggtgc
     1321 ttttttttct cgaggtacct ctctacatat gacatgtgag caaaaggcca gcaaaaggcc
     1381 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     1441 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     1501 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     1561 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     1621 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     1681 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     1741 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     1801 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
     1861 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     1921 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     1981 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     2041 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     2101 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     2161 tggtctgaca gtttgccgac taccttggtg
//