LOCUS BC166-(pHU6.Ch13_102010 2191 bp ds-DNA circular 30-AUG-2016 DEFINITION . ACCESSION urn.local...bn-6f8p62z VERSION urn.local...bn-6f8p62z FEATURES Location/Qualifiers primer_bind complement(248..267) /label="NMG-18" /ApEinfo_revcolor=#c7b0e3 /ApEinfo_fwdcolor=#c7b0e3 promoter 644..900 /label="hU6 prom" /ApEinfo_revcolor=#ff9ccd /ApEinfo_fwdcolor=#ff9ccd misc_feature 908..928 /label="Ch13_102010574-gRNA-rev" /ApEinfo_revcolor=#faac61 /ApEinfo_fwdcolor=#faac61 promoter complement(495..523) /label="amp prom" /ApEinfo_revcolor=#d59687 /ApEinfo_fwdcolor=#d59687 misc_feature 1012..1012 /label="" /ApEinfo_revcolor=#d6b295 /ApEinfo_fwdcolor=#d6b295 primer_bind 929..1011 /label="Spy gRNA hairpins (constant)" /ApEinfo_revcolor=#f8d3a9 /ApEinfo_fwdcolor=#f8d3a9 misc_feature complement(1082..1701) /label="pBR322 origin" /ApEinfo_revcolor=#b7e6d7 /ApEinfo_fwdcolor=#b7e6d7 mat_peptide complement(1856..453) /label="aadA (Spec Resistance)" /ApEinfo_revcolor=#b7e6d7 /ApEinfo_fwdcolor=#b7e6d7 misc_feature 1858..448 /label="ACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATA" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e misc_feature 1013..1013 /label="AGCTTGGGCCG" /ApEinfo_revcolor=#f8d3a9 /ApEinfo_fwdcolor=#f8d3a9 source complement(1856..453) /label="source" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 misc_feature 909..928 /label="AGACGATTAATGCGTCTC" /ApEinfo_revcolor=#9eafd2 /ApEinfo_fwdcolor=#9eafd2 ORIGIN 1 ctccgccgct ggacctacca aggcaacgct atgttctctt gcttttgtca gcaagatagc 61 cagatcaatg tcgatcgtgg ctggctcgaa gatacctgca agaatgtcat tgcgctgcca 121 ttctccaaat tgcagttcgc gcttagctgg ataacgccac ggaatgatgt cgtcgtgcac 181 aacaatggtg acttctacag cgcggagaat ctcgctctct ccaggggaag ccgaagtttc 241 caaaaggtcg ttgatcaaag ctcgccgcgt tgtttcatca agccttacgg tcaccgtaac 301 cagcaaatca atatcactgt gtggcttcag gccgccatcc actgcggagc cgtacaaatg 361 tacggccagc aacgtcggtt cgagatggcg ctcgatgacg ccaactacct ctgatagttg 421 agtcgatact tcggcgatca ccgcttccct catactcttc ctttttcaat attattgaag 481 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa 541 acaaatatgg gttccgcgca catttccccg aaaagtgcca cctgacgtcg ctagctgtac 601 aaaaaagcag gctttaaagg aaccaattca ttcgactgga tccaaggtcg ggcaggaaga 661 gggcctattt cccatgattc cttcatattt gcatatacga tacaaggctg ttagagagat 721 aattagaatt aatttgactg taaacacaaa gatattagta caaaatacgt gacgtagaaa 781 gtaataattt cttgggtagt ttgcagtttt aaaattatgt tttaaaatgg actatcatat 841 gcttaccgta acttgaaagt atttcgattt cttggcttta tatatcttgt ggaaaggacg 901 aaacaccgtc agctatgatc acttccctgt tttagagcta gaaatagcaa gttaaaataa 961 ggctagtccg ttatcaactt gaaaaagtgg caccgagtcg gtgctttttt ttctcgaggt 1021 acctctctac atatgacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg 1081 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac 1141 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg 1201 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct 1261 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg 1321 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct 1381 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac 1441 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt 1501 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc 1561 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca 1621 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat 1681 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac 1741 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt 1801 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagtttgc 1861 cgactacctt ggtgatctcg cctttcacgt agtggacaaa ttcttccaac tgatctgcgc 1921 gcgaggccaa gcgatcttct tcttgtccaa gataagcctg tctagcttca agtatgacgg 1981 gctgatactg ggccggcagg cgctccattg cccagtcggc agcgacatcc ttcggcgcga 2041 ttttgccggt tactgcgctg taccaaatgc gggacaacgt aagcactaca tttcgctcat 2101 cgccagccca gtcgggcggc gagttccata gcgttaaggt ttcatttagc gcctcaaata 2161 gatcctgttc aggaaccgga tcaaagagtt c //