LOCUS       BC04-pCALNL-GFP-        5607 bp    DNA     circular UNA 17-MAR-2017
DEFINITION  .
ACCESSION   urn.local...f-7c7v9w3
VERSION     urn.local...f-7c7v9w3
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     primer_bind     20..49
                     /label="FwdCMV/Promoter"
     promoter        383..662
                     /note="Geneious type: Promoter"
                     /note="Geneious type: promoter"
                     /label="cBA prom"
     misc_feature    1745..1766
                     /note="Geneious type: Editing History Replacement"
                     /label="ATAACTTCGTATAGCATACATTATACGAAGTTATCTCGAGTCGGATTTGA
                     TCTGATCAAGAGACAGGATGAGGATCGTTTCGCATGATTGAACAAGATGGATTGCACG
                     CAGGTTCTCCGGCCGCTTGGGTGGAGAGGCTATTCGGCTATGACTGGGCACAACAGAC
                     AATCGGCTGCTCTGATGCCGCCGTGTTCCGGCTGTCAGCGCAGGGGCGCCCGGTTCTT
                     TTTGTCAAGACCGACCTGTCCGGTGCCCTGAATGAACTGCAGGACGAGGCAGCGCGGC
                     TATCGTGGCTGGCCACGACGGGCGTTCCTTGCGCAGCTGTGCTCGACGTTGTCACTGA
                     AGCGGGAAGGGACTGGCTGCTATTGGGCGAAGTGCCGGGGCAGGATCTCCTGTCATCT
                     CACCTTGCTCCTGCCGAGAAAGTATCCATCATGGCTGATGCAATGCGGCGGCTGCATA
                     CGCTTGATCCGGCTACCTGCCCATTCGACCACCAAGCGAAACATCGCATCGAGCGAGC
                     ACGTACTCGGATGGAAGCCGGTCTTGTCGATCAGGATGATCTGGACGAAGAGCATCAG
                     GGGCTCGCGCCAGCCGAACTGTTCGCCAGGCTCAAGGCGCGCATGCCCGACGGCGAGG
                     ATCTCGTCGTGACCCATGGCGATGCCTGCTTGCCGAATATCATGGTGGAAAATGGCCG
                     CTTTTCTGGATTCATCGACTGTGGCCGGCTGGGTGTGGCGGACCGCTATCAGGACATA
                     GCGTTGGCTACCCGTGATATTGCTGAAGAGCTTGGCGGCGAATGGGCTGACCGCTTCC
                     TCGTGCTTTACGGTATCGCCGCTCCCGATTCGCAGCGCATCGCCTTCTATCGCCTTCT
                     TGACGAGTTCTTCTGAGCGGGACTCTGGGGTTCGAAATGACCGACCAAGCGACGCCCA
                     ACCTGCCATCACGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGGTTGGGCTTCGG
                     AATCGTTTTCCGGGACGCCGGCTGGATGATCCTCCAGCGCGGGGATCTCATGCTGGAG
                     TTCTTCGCCCACCCCATCGATAACTTGTTTATTGCAGCTTATAATGGTTACAAATAAA
                     GCAATAGCATCACAAATTTCACAAATAAAGCATTTTTTTCACTGCATTCTAGTTGTGG
                     TTTGTCCAAACTCATCAATGTATCTTATCATGTCTGGATCAAATCCGAACGCGTATAA
                     CTTCGTATAGCATACATTATACGAAGTTAT"
     misc_feature    complement(1746..1751)
                     /label="Esp31"
     misc_feature    1760..1765
                     /label="Esp31"
     primer_bind     1844..1863
                     /label="Primer3"
     gene            1847..2560
                     /label="EGFP"
     terminator      2570..3094
                     /note="Geneious type: Terminator"
                     /note="Geneious type: terminator"
                     /label="rb_glob_PA term"
     misc_feature    3349..3462
                     /note="Geneious type: Editing History Replacement"
                     /label="TCCCGCCCCTAACTCCGCCCAGTTCCGCCCATTCTCCGCCCCATGGCTGA
                     CTAATTTTTTTTATTTATGCAGAGGCCGAGGCCGCCTCGGCCTCTGAGCTATTCCAGA
                     AGTAGTGAGGAGGCTTTTTTGGAGG"
     promoter        <3463..3473
                     /note="Geneious type: Promoter"
                     /note="Geneious type: promoter"
                     /label="SV40 prom"
     terminator      3487..3606
                     /label="SV40 PA terminator"
     misc_feature    3515..3565
                     /label="SV40\polyA"
     misc_feature    complement(3575..3594)
                     /note="Geneious type: primer_bind_reverse"
                     /label="EBV-rev-primer"
     misc_feature    complement(3842..4461)
                     /note="Geneious type: Origin of Replication"
                     /note="Geneious type: origin of replication"
                     /label="pBR322 origin"
     misc_feature    complement(3857..4445)
                     /label="pMB1 origin of replication"
     misc_feature    complement(4616..5476)
                     /note="Geneious type: Marker"
                     /note="Geneious type: marker"
                     /label="amp marker"
     gene            complement(4616..5476)
                     /gene="bla"
                     /label="bla gene"
     misc_feature    complement(4616..5476)
                     /label="AmpR"
     sig_peptide     complement(5408..5476)
                     /gene="bla"
                     /note="Required for secretion to the periplasm; cleaved
                     off to form the mature beta-lactamase protein; cleaved off
                     to form the mature beta-lactamase protein.; required for
                     secretion to the periplasm"
                     /label="bla signal peptide"
     promoter        complement(5518..5546)
                     /note="Geneious type: Promoter"
                     /note="Geneious type: promoter"
                     /label="AmpR promoter; amp prom"
ORIGIN      
        1 gtcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      181 ggactttcca ttgacgtcaa tgggtggact atttacggta aactgcccac ttggcagtac
      241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
      301 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
      361 tattagtcat cgctattacc atgggtcgag gtgagcccca cgttctgctt cactctcccc
      421 atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca
      481 gcgatggggg cggggggggg gggggcgcgc gccaggcggg gcggggcggg gcgaggggcg
      541 gggcggggcg aggcggagag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt
      601 tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc
      661 gggagtcgct gcgttgcctt cgccccgtgc cccgctccgc gccgcctcgc gccgcccgcc
      721 ccggctctga ctgaccgcgt tactcccaca ggtgagcggg cgggacggcc cttctcctcc
      781 gggctgtaat tagcgcttgg tttaatgacg gctcgtttct tttctgtggc tgcgtgaaag
      841 ccttaaaggg ctccgggagg gccctttgtg cgggggggag cggctcgggg ggtgcgtgcg
      901 tgtgtgtgtg cgtggggagc gccgcgtgcg gcccgcgctg cccggcggct gtgagcgctg
      961 cgggcgcggc gcggggcttt gtgcgctccg cgtgtgcgcg aggggagcgc ggccgggggc
     1021 ggtgccccgc ggtgcggggg ggctgcgagg ggaacaaagg ctgcgtgcgg ggtgtgtgcg
     1081 tgggggggtg agcagggggt gtgggcgcgg cggtcgggct gtaacccccc cctgcacccc
     1141 cctccccgag ttgctgagca cggcccggct tcgggtgcgg ggctccgtgc ggggcgtggc
     1201 gcggggctcg ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg cggggcgggg
     1261 ccgcctcggg ccggggaggg ctcgggggag gggcgcggcg gccccggagc gccggcggct
     1321 gtcgaggcgc ggcgagccgc agccattgcc ttttatggta atcgtgcgag agggcgcagg
     1381 gacttccttt gtcccaaatc tggcggagcc gaaatctggg aggcgccgcc gcaccccctc
     1441 tagcgggcgc gggcgaagcg gtgcggcgcc ggcaggaagg aaatgggcgg ggagggcctt
     1501 cgtgcgtcgc cgcgccgccg tccccttctc catctccagc ctcggggctg ccgcaggggg
     1561 acggctgcct tcggggggga cggggcaggg cggggttcgg cttctggcgt gtgaccggcg
     1621 gctctagagc ctctgctaac catgttcatg ccttcttctt tttcctacag ctcctgggca
     1681 acgtgctggt tattgtgctg tctcatcatt ttggcaaaga attgattaat tcgagcgaac
     1741 gcgttgagac gctgccatcc gtctcgctcg agtcgctcgg tacgatttaa attgaattct
     1801 gcagtcgacg gtaccgcggg cccgggatcc accggtcgcc accatggtga gcaagggcga
     1861 ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca
     1921 caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa
     1981 gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac
     2041 ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa
     2101 gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa
     2161 ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct
     2221 gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta
     2281 caacagccac aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt
     2341 caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa
     2401 cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc
     2461 cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac
     2521 cgccgccggg atcactctcg gcatggacga gctgtacaag taaagcggcc gcactcctca
     2581 ggtgcaggct gcctatcaga aggtggtggc tggtgtggcc aatgccctgg ctcacaaata
     2641 ccactgagat ctttttccct ctgccaaaaa ttatggggac atcatgaagc cccttgagca
     2701 tctgacttct ggctaataaa ggaaatttat tttcattgca atagtgtgtt ggaatttttt
     2761 gtgtctctca ctcggaagga catatgggag ggcaaatcat ttaaaacatc agaatgagta
     2821 tttggtttag agtttggcaa catatgccat atgctggctg ccatgaacaa aggtggctat
     2881 aaagaggtca tcagtatatg aaacagcccc ctgctgtcca ttccttattc catagaaaag
     2941 ccttgacttg aggttagatt ttttttatat tttgttttgt gttatttttt tctttaacat
     3001 ccctaaaatt ttccttacat gttttactag ccagattttt cctcctctcc tgactactcc
     3061 cagtcatagc tgtccctctt ctcttatgaa gatccctcga cctgcagccc aagcttggcg
     3121 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
     3181 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
     3241 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagcggatc
     3301 cgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccagt tccgcccatt
     3361 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct
     3421 ctgagctatt ccagaagtag tgaggaggct tttttggagg ttcctaggct tttgcaaaaa
     3481 gctaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
     3541 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     3601 tcttatcatg tctggatccg ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
     3661 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     3721 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     3781 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     3841 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     3901 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     3961 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     4021 ttctcccttc gggaagcgtg gcgctttctc aatgctcacg ctgtaggtat ctcagttcgg
     4081 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     4141 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     4201 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     4261 tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc
     4321 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     4381 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     4441 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     4501 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     4561 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     4621 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     4681 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     4741 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     4801 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     4861 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     4921 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     4981 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     5041 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     5101 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     5161 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     5221 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     5281 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     5341 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     5401 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     5461 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     5521 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     5581 gcacatttcc ccgaaaagtg ccacctg
//
