LOCUS       pBS\3A.1\mitfa        4534 bp    DNA     circular SYN  6-JUL-2010
DEFINITION  pBluescript SK(-) vector DNA, phagemid excised from lambda ZAP.
ACCESSION   X52324
KEYWORDS    -.
SOURCE      -.
  ORGANISM  Cloning vector
REFERENCE   [1]
  AUTHORS   Short,J.M., Fernandez,J.M., Sorge,J.A. and Huse,W.D.
  JOURNAL   Nucleic Acids Res. 16, 7583-7600 (1988)
REFERENCE   [2]
  AUTHORS   Alting-Mees,M.A. and Short,J.M.
  JOURNAL   Nucleic Acids Res. 17, 9494-9494 (1989)
REFERENCE   [3]
  AUTHORS   Thomas,E.A.
  JOURNAL   Unpublished (1990)
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDBDATE|559040731|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pBS 3A.1 mitfa|
COMMENT     VNTAUTHORNAME|James Lister|
COMMENT     VNTAUTHORTEL|(804)628-4518|
COMMENT     VNTAUTHOREML|jalister@vcu.edu|
COMMENT     VNTOAUTHORNAME|UNKNOWN|
FEATURES             Location/Qualifiers
     rep_origin      6..462
                     /vntifkey="33"
                     /label=f1\(-)\origin
     rep_origin      2608..3488
                     /vntifkey="33"
                     /label=ColE1\origin
     CDS             complement(3551..4408)
                     /vntifkey="4"
                     /label=ampicillin
     misc_feature    complement(674..2268)
                     /vntifkey="21"
                     /label=1\to\1595\of\nacre\cDNA
     misc_feature    complement(2269..2276)
                     /vntifkey="21"
                     /label=library\adapter
     CDS             complement(950..2188)
                     /vntifkey="4"
                     /label=mitfa
     promoter        625..646
                     /vntifkey="30"
                     /label=T7
     promoter        complement(2348..2367)
                     /vntifkey="30"
                     /label=T3
BASE COUNT     1046 a      1120 c      1121 g      1247 t 
ORIGIN
        1 cacctgacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg 
       61 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc 
      121 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc 
      181 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta 
      241 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta 
      301 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg 
      361 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa 
      421 aatttaacgc gaattttaac aaaatattaa cgcttacaat ttccattcgc cattcaggct 
      481 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa 
      541 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg 
      601 ttgtaaaacg acggccagtg aattgtaata cgactcacta tagggcgaat tgggtaccgg 
      661 gccccccctc gagttttttt tttttttttt tattcaagct cctctttata tcatccgcga 
      721 gaatccttca aaaatcaact aattgtttac acgttttcat tcttgactag actctgacat 
      781 gataaatgaa aaaatatata cacaagcaca ctaaatattc tgaaatacaa gattaatact 
      841 ttacgaaatt gcgctcttca tgatctggat gtaacactgg tacttacaag acacacacac 
      901 acacaaatct aattgtctaa ctttggttca tgaaatttag ttggcattgc taacagccat 
      961 tgtcatgttc gtccatactg ctgctgccgc tactgttgga aggtactggg gatccggagg 
     1021 acaacagcgg gtcgctgctg cccaccggag acagggtgtt gtccataagc atgtcctcca 
     1081 gcttggtgga ggccttgttt gggctgtcgt agacccccgg ctcgccgtcc tccgtgggac 
     1141 tgtcattgta gctgatggtg ccgttgttga ggtccagagt ggtaggacgg gacatgtcag 
     1201 gactgggaag gtgtgggtac aaattggatg tgcagtcccc catcccaggc tcttgtttta 
     1261 ttgctcgagc cactaactca gcggaataaa ggctggaaga agctacaacg gtgagtccat 
     1321 gagcacgggc ttgcatttca agttcctgaa tacggagcag gagatgtctg tttgcgtgtt 
     1381 ctagtctctt ctgtctgttt tccagctctt ttgctttctg ctgctctttc tgcaatttcc 
     1441 taatgtaatc cactgatgct ttcaggatgg tgcctttatt ccacctcatg tctggatcat 
     1501 ttgacttggg aattaaagtc cccagctcct taattctgtc attgatatta aaccgccttc 
     1561 ttcgttcaat gaggttgtgg ttgtccttct tttgtctctc ttttgccagg gctctgactt 
     1621 ctgcttctac tggaaagcca tcaggtcttt gataagagtc aaacttgcca catggtccag 
     1681 ctttgtccat aatgtgcatc atgcccggag atggagtaac ggataattcc cttttgacgg 
     1741 ccggcaggct tgaggggcag gagttactga tggaaactcc agctggagga agagcatgat 
     1801 tgctgtacat gtccagcagg tttgctgaaa ctggaatcgt gtttgtcatt tgaagtcctg 
     1861 catccatgaa cccaagaatg tcatcactgt agcttgattc caaacttata atgtcttcaa 
     1921 ttacatcgtc catctctttc tcacagttga gggtgagaag ggccataggg ctgttgggag 
     1981 cgctggctcc gggtcccggg gtcatgccgt gctcggcggg ctggctgggg ccgggccctg 
     2041 tgctggcctg tgggctcagc ttggctccca gtgcactgga caggtagtgc ttcacctgct 
     2101 gcctctggct ctgctggatg tggtacttcg agggggtttc caggtgggtc tgaacctggt 
     2161 agtgactgta ctcgagcatc tccaacatgt tcaactatgt gttagcttca aaaaagtatt 
     2221 ccctttgtga atttaggtga tgtgcaagaa ctgaccagtc gtcttggcct cgtgccgaat 
     2281 tcctgcagcc cgggggatcc actagttcta gagcggccgc caccgcggtg gagctccagc 
     2341 ttttgttccc tttagtgagg gttaatttcg agcttggcgt aatcatggtc atagctgttt 
     2401 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag 
     2461 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg 
     2521 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg 
     2581 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc 
     2641 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc 
     2701 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg 
     2761 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat 
     2821 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag 
     2881 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga 
     2941 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg 
     3001 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt 
     3061 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac 
     3121 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc 
     3181 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt 
     3241 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc 
     3301 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc 
     3361 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg 
     3421 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag 
     3481 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg 
     3541 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt 
     3601 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca 
     3661 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca 
     3721 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc 
     3781 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt 
     3841 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg 
     3901 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc 
     3961 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg 
     4021 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga 
     4081 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga 
     4141 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta 
     4201 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg 
     4261 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact 
     4321 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata 
     4381 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt 
     4441 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa 
     4501 ataggggttc cgcgcacatt tccccgaaaa gtgc 
//
