LOCUS       pCS2+MTmitfa        5855 bp    DNA     circular SYN  6-JAN-2006
DEFINITION  -.
ACCESSION   -
KEYWORDS    -.
SOURCE      -.
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDBDATE|398679564|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pCS2+MTmitfa|
COMMENT     VNTAUTHORNAME|James Lister|
COMMENT     VNTAUTHORTEL|(804)628-4518|
COMMENT     VNTAUTHOREML|jalister@vcu.edu|
COMMENT     VNTOAUTHORNAME|UNKNOWN|
FEATURES             Location/Qualifiers
     promoter        35..51
                     /vntifkey="30"
                     /label=SP6
     frag            100..350
                     /vntifkey="21"
                     /label=6\myc\tag\repeats
                     /note="Unknown feature type:frag"
     promoter        complement(1883..1899)
                     /vntifkey="30"
                     /label=T7
     polyA_site      1904..2099
                     /vntifkey="26"
                     /label=SV40\polyA
     promoter        complement(2153..2169)
                     /vntifkey="30"
                     /label=T3
     primer          2186..2201
                     /vntifkey="27"
                     /label=M13\Reverse
     rep_origin      complement(2451..2696)
                     /vntifkey="33"
                     /label=ColE1\ori
     CDS             complement(3354..4211)
                     /vntifkey="4"
                     /label=ampicillin
     rep_origin      complement(4340..4800)
                     /vntifkey="33"
                     /label=f1\ori
     promoter        4869..>5855
                     /vntifkey="29"
                     /label=sCMV\IE94\promoter
     CDS             >367..1866
                     /vntifkey="4"
                     /label=mitfa
BASE COUNT     1597 a      1408 c      1420 g      1430 t 
ORIGIN
        1 cgccattctg cctggggacg tcggagcaag cttgatttag gtgacactat agaatacaag 
       61 ctacttgttc tttttgcagg atcccatcga tttaaagcta tggagcaaaa gctcatttct 
      121 gaagaggact tgaatgaaat ggagcaaaag ctcatttctg aagaggactt gaatgaaatg 
      181 gagcaaaagc tcatttctga agaggacttg aatgaaatgg agcaaaagct catttctgaa 
      241 gaggacttga atgaaatgga gcaaaagctc atttctgaag aggacttgaa tgaaatggag 
      301 agcttgggcg acctcaccat ggagcaaaag ctcatttctg aagaggactt gaattcaagg 
      361 cctctcgagt acagtcacta ccaggttcag acccacctgg aaaccccctc gaagtaccac 
      421 atccagcaga gccagaggca gcaggtgaag cactacctgt ccagtgcact gggagccaag 
      481 ctgagcccac aggccagcac agggcccggc cccagccagc ccgccgagca cggcatgacc 
      541 ccgggacccg gagccagcgc tcccaacagc cctatggccc ttctcaccct caactgtgag 
      601 aaagagatgg acgatgtaat tgaagacatt ataagtttgg aatcaagcta cagtgatgac 
      661 attcttgggt tcatggatgc aggacttcaa atgacaaaca cgattccagt ttcagcaaac 
      721 ctgctggaca tgtacagcaa tcatgctctt cctccagctg gagtttccat cagtaactcc 
      781 tgcccctcaa gcctgccggc cgtcaaaagg gaattatccg ttactccatc tccgggcatg 
      841 atgcacatta tggacaaagc tggaccatgt ggcaagtttg actcttatca aagacctgat 
      901 ggctttccag tagaagcaga agtcagagcc ctggcaaaag agagacaaaa gaaggacaac 
      961 cacaacctca ttgaacgaag aaggcggttt aatatcaatg acagaattaa ggagctgggg 
     1021 actttaattc ccaagtcaaa tgatccagac atgaggtgga ataaaggcac catcctgaaa 
     1081 gcatcagtgg attacattag gaaattgcag aaagagcagc agaaagcaaa agagctggaa 
     1141 aacagacaga agagactaga acacgcaaac agacatctcc tgctccgtat tcaggaactt 
     1201 gaaatgcaag cccgtgctca tggactcacc gttgtagctt cttccagcct ttattccgct 
     1261 gagttagtgg ctcgagcaat aaaacaagag cctgggatgg gggactgcac atccaatttg 
     1321 tacccacacc ttcccagtcc tgacatgtcc cgtcctacca ctctggacct caacaacggc 
     1381 accatcagct acaatgacag tcccacggag gacggcgagc cgggggtcta cgacagccca 
     1441 aacaaggcct ccaccaagct ggaggacatg cttatggaca acaccctgtc tccggtgggc 
     1501 agcagcgacc cgctgttgtc ctccggatcc ccagtacctt ccaacagtag cggcagcagc 
     1561 agtatggacg aacatgacaa tggctgttag caatgccaac taaatttcat gaaccaaagt 
     1621 tagacaatta gatttgtgtg tgtgtgtgtc ttgtaagtac cagtgttaca tccagatcat 
     1681 gaagagcgca atttcgtaaa gtattaatct tgtatttcag aatatttagt gtgcttgtgt 
     1741 atatattttt tcatttatca tgtcagagtc tagtcaagaa tgaaaacgtg taaacaatta 
     1801 gttgattttt gaaggattct cgcggatgat ataaagagga gcttgaataa aaaaaaaaaa 
     1861 aaaaaactcg agcctctaga actatagtga gtcgtattac gtagatccag acatgataag 
     1921 atacattgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg 
     1981 tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa 
     2041 caacaacaat tgcattcatt ttatgtttca ggttcagggg gaggtgtggg aggtttttta 
     2101 attcgcggcc gcggcgccaa tgcattgggc ccggtaccca gcttttgttc cctttagtga 
     2161 gggttaattg cgcgcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat 
     2221 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc 
     2281 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga 
     2341 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt 
     2401 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg 
     2461 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac 
     2521 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg 
     2581 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca 
     2641 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc 
     2701 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc 
     2761 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag 
     2821 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc 
     2881 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca 
     2941 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg 
     3001 aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg 
     3061 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct 
     3121 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa 
     3181 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa 
     3241 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa 
     3301 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc 
     3361 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga 
     3421 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca 
     3481 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc 
     3541 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat 
     3601 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc 
     3661 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt 
     3721 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc 
     3781 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg 
     3841 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt 
     3901 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg 
     3961 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga 
     4021 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg 
     4081 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg 
     4141 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt 
     4201 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc 
     4261 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca 
     4321 tttccccgaa aagtgccacc taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa 
     4381 atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata 
     4441 aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac 
     4501 tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc 
     4561 cactacgtga accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa 
     4621 atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg 
     4681 cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg 
     4741 tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtccc 
     4801 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat 
     4861 tacgccagtc gaccatagcc aattcaatat ggcgtatatg gactcatgcc aattcaatat 
     4921 ggtggatctg gacctgtgcc aattcaatat ggcgtatatg gactcgtgcc aattcaatat 
     4981 ggtggatctg gaccccagcc aattcaatat ggcggacttg gcaccatgcc aattcaatat 
     5041 ggcggacttg gcactgtgcc aactggggag gggtctactt ggcacggtgc caagtttgag 
     5101 gaggggtctt ggccctgtgc caagtccgcc atattgaatt ggcatggtgc caataatggc 
     5161 ggccatattg gctatatgcc aggatcaata tataggcaat atccaatatg gccctatgcc 
     5221 aatatggcta ttggccaggt tcaatactat gtattggccc tatgccatat agtattccat 
     5281 atatgggttt tcctattgac gtagatagcc cctcccaatg ggcggtccca tataccatat 
     5341 atggggcttc ctaataccgc ccatagccac tcccccattg acgtcaatgg tctctatata 
     5401 tggtctttcc tattgacgtc atatgggcgg tcctattgac gtatatggcg cctcccccat 
     5461 tgacgtcaat tacggtaaat ggcccgcctg gctcaatgcc cattgacgtc aataggacca 
     5521 cccaccattg acgtcaatgg gatggctcat tgcccattca tatccgttct cacgccccct 
     5581 attgacgtca atgacggtaa atggcccact tggcagtaca tcaatatcta ttaatagtaa 
     5641 cttggcaagt acattactat tggaaggacg ccagggtaca ttggcagtac tcccattgac 
     5701 gtcaatggcg gtaaatggcc cgcgatggct gccaagtaca tccccattga cgtcaatggg 
     5761 gaggggcaat gacgcaaatg ggcgttccat tgacgtaaat gggcggtagg cgtgcctaat 
     5821 gggaggtcta tataagcaat gctcgtttag ggaac 
//
