LOCUS       Construct_3            10776 bp    DNA     circular UNA 01-AUG-2016
DEFINITION  U6p_20nt1_mod2_sgRNA (stgRNA).
ACCESSION   urn.local...1461188663766.11
VERSION     urn.local...1461188663766.11
KEYWORDS    .
SOURCE      Human
  ORGANISM  Human
            .
FEATURES             Location/Qualifiers
     promoter        239..815
                     /created_by="User"
                     /modified_by="User"
                     /label="CMV_imm_early promoter"
     misc_feature    318..605
                     /created_by="User"
                     /label="CAG enhancer"
     LTR             835..1015
                     /created_by="User"
                     /modified_by="User"
                     /label="HIV-1_5_LTR"
     misc_feature    1126..1170
                     /created_by="User"
                     /modified_by="User"
                     /label="HIV-1_psi_pack"
     misc_feature    1680..1913
                     /created_by="User"
                     /label="RRE"
     misc_feature    2444..2459
                     /created_by="User"
                     /label="cPPT"
     primer_bind     2571..2590
                     /Mismatches=0
                     /created_by="primer3"
                     /annotation_group="141566513778868200028694d1d-6973-402e-b
                     821-c28ce9b7852b"
                     /%GC="55.0"
                     /Tm="59.1"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="GGGACAGCAGAGATCCAGTT"
                     /label="JP1706"
     primer_bind     2577..2607
                     /Mismatches=0
                     /created_by="primer3"
                     /annotation_group="14158103166042940003272ff1b-6c61-4f45-a
                     fa8-195ac9b99404"
                     /%GC="64.5"
                     /Tm="75.2"
                     /Hairpin_Tm="47.7"
                     /Self_Dimer_Tm="8.8"
                     /Sequence="GCAGAGATCCAGTTTGGGGGGTTCCGCGCAC"
                     /label="JP1710"
     primer_bind     2594..2616
                     /created_by="primer3"
                     /%GC="69.6"
                     /Tm="64.72"
                     /Hairpin="4.0"
                     /Primer-Dimer="4.0"
                     /Monovalent_Salt_Concentration="50.0"
                     /Divalent_Salt_Concentration="0.0"
                     /DNTP_Concentration="0.0"
                     /Sequence="GGGGTTCCGCGCACATTTCCCCG"
                     /Mismatches=0
                     /annotation_group="PrimerPlugin
                     Group3c68cbfa-121b-4872-a7ab-08a32877e25e_urn:local:.:1365
                     712513487.384"
                     /label="JP313"
     CDS             complement(<2594..2598)
                     /gene="AMP"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
                     /label="AMP CDS"
     promoter        2630..2940
                     /created_by="User"
                     /modified_by="User"
                     /label="U6 promoter"
     misc_feature    2727..2734
                     /created_by="User"
                     /label="Enhancer"
     misc_feature    2883..2901
                     /created_by="User"
                     /label="proximal element"
     misc_feature    2919..2924
                     /created_by="User"
                     /label="TATA box"
     gRNA            2949..2967
                     /created_by="User"
                     /modified_by="User"
                     /label="20nt1"
     misc_feature    2968..3044
                     /created_by="User"
                     /modified_by="User"
                     /label="stgRNA scaffold"
     modified_base   2969..2970
                     /created_by="User"
                     /modified_by="User"
                     /label="TT->GG"
     modified_base   2995..2996
                     /created_by="User"
                     /label="AA->CC"
     terminator      3045..3049
                     /created_by="User"
                     /label="Termination signal"
     misc_feature    complement(3074..3105)
                     /Mismatches=0
                     /created_by="primer3"
                     /annotation_group="141581031682964600096881a94-d9ce-450e-9
                     300-407e04f37bb8"
                     /%GC="53.1"
                     /Tm="69.7"
                     /Hairpin_Tm="30.8"
                     /Self_Dimer_Tm="11.5"
                     /Sequence="CCCGGTAGAATTCCTCGACGTCTAATGCCAAC"
                     /label="JP1711"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(3087..3106)
                     /Mismatches=0
                     /created_by="primer3"
                     /annotation_group="1415665137960342000e062635e-3225-4b00-a
                     339-35365797f3ae"
                     /%GC="55.0"
                     /Tm="58.9"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="12.3"
                     /Sequence="ACCCGGTAGAATTCCTCGAC"
                     /label="JP1707"
                     /note="Geneious type: primer_bind_reverse"
     CDS             3094..3609
                     /gene="PGKp"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
                     /label="PGKp CDS"
     misc_feature    complement(3169..3193)
                     /Hairpin="6.0"
                     /Primer-Dimer="2.0"
                     /Monovalent_Salt_Concentration="50.0"
                     /Divalent_Salt_Concentration="0.0"
                     /DNTP_Concentration="0.0"
                     /Mismatches=0
                     /created_by="primer3"
                     /annotation_group="14158097666895440008bddd82b-4d20-4bb2-8
                     efa-9fa8e0328024"
                     /%GC="68.0"
                     /Tm="71.6"
                     /Hairpin_Tm="64.2"
                     /Self_Dimer_Tm="21.7"
                     /Sequence="GGCCAGAGGCCACTTGTGTAGCGCC"
                     /label="JP467"
                     /note="Geneious type: primer_bind_reverse"
     misc_signal     order(3620..3628,3620..3628)
                     /created_by="User"
                     /label="KOZAK"
     CDS             3629..4342
                     /gene="EBFP2"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
                     /modified_by="User"
                     /label="EBFP2 CDS"
     sig_peptide     4343..4408
                     /created_by="User"
                     /modified_by="User"
                     /label="p2a"
     CDS             4409..5440
                     /created_by="User"
                     /label="hygroR"
     misc_feature    5470..6057
                     /created_by="User"
                     /label="WPRE"
     misc_feature    6244..6265
                     /created_by="User"
                     /modified_by="User"
                     /label="U3PPt"
     misc_feature    6244..6259
                     /created_by="User"
                     /label="cPPT"
     LTR             6581..6761
                     /created_by="User"
                     /label="truncHIV-1_3_LTR"
     terminator      6790..7017
                     /created_by="User"
                     /label="bGH_PA_terminator"
     rep_origin      7080..7386
                     /created_by="User"
                     /label="f1_origin"
     misc_feature    complement(7506..7721)
                     /created_by="User"
                     /label="SV40 enhancer"
     rep_origin      7685..7762
                     /created_by="User"
                     /label="SV40 origin"
     promoter        7880..7947
                     /created_by="User"
                     /label="EM7 promoter"
     gene            7948..8319
                     /created_by="User"
                     /modified_by="User"
                     /label="bleo"
     terminator      8455..8574
                     /created_by="User"
                     /label="SV40_PA_terminator"
     promoter        complement(8671..8700)
                     /created_by="User"
                     /modified_by="User"
                     /label="lac_promoter"
     CDS             complement(9783..10643)
                     /created_by="User"
                     /label="AmpR"
     promoter        complement(10685..10712)
                     /created_by="User"
                     /label="AmpR promoter"
ORIGIN      
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggggggttc cgcgcacatt tccccgaaaa gtgccacctt gtacaaaaaa
     2641 gcaggcttta aaggaaccaa ttcagtcgac tggatccggt accaaggtcg ggcaggaaga
     2701 gggcctattt cccatgattc cttcatattt gcatatacga tacaaggctg ttagagagat
     2761 aattagaatt aatttgactg taaacacaaa gatattagta caaaatacgt gacgtagaaa
     2821 gtaataattt cttgggtagt ttgcagtttt aaaattatgt tttaaaatgg actatcatat
     2881 gcttaccgta acttgaaagt atttcgattt cttggcttta tatatcttgt ggaaaggacg
     2941 aaacaccgta agtcggagta ctgtcctggg ttagagctag aaatagcaag ttaacctaag
     3001 gctagtccgt tatcaacttg aaaaagtggc accgagtcgg tgcttttttc tagacccagc
     3061 tttcttgtac aaagttggca ttagacgtcg aggaattcta ccgggtaggg gaggcgcttt
     3121 tcccaaggca gtctggagca tgcgctttag cagccccgct gggcacttgg cgctacacaa
     3181 gtggcctctg gcctcgcaca cattccacat ccaccggtag gcgccaaccg gctccgttct
     3241 ttggtggccc cttcgcgcca ccttctactc ctcccctagt caggaagttc ccccccgccc
     3301 cgcagctcgc gtcgtgcagg acgtgacaaa tggaagtagc acgtctcact agtctcgtgc
     3361 agatggacag caccgctgag caatggaagc gggtaggcct ttggggcagc ggccaatagc
     3421 agctttgctc cttcgctttc tgggctcaga ggctgggaag gggtgggtcc gggggcgggc
     3481 tcaggggcgg gctcaggggc ggggcgggcg cccgaaggtc ctccggaggc ccggcattct
     3541 gcacgcttca aaagcgcacg tctgccgcgc tgttctcctc ttcctcatct ccgggccttt
     3601 cgacctgcat cccaagcttg ccaccatggt gagcaagggc gaggagctgt tcaccggggt
     3661 ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgagggg
     3721 cgagggcgag ggcgatgcca ccaacggcaa gctgaccctg aagttcatct gcaccaccgg
     3781 caagctgccc gtgccctggc ccaccctcgt gaccaccctg agccacggcg tgcagtgctt
     3841 cgcccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg
     3901 ctacgtccag gagcgcacca tcttcttcaa ggacgacggc acctacaaga cccgcgccga
     3961 ggtgaagttc gagggcgaca ccctagtgaa ccgcatcgag ctgaagggcg tcgacttcaa
     4021 ggaggacggc aacatcctgg ggcacaagct ggagtacaac ttcaacagcc acaacatcta
     4081 tatcatggcc gtcaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacgt
     4141 ggaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg
     4201 ccccgtgctg ctgcccgaca gccactacct gagcacccag tccgtgctga gcaaagaccc
     4261 caacgagaag cgcgatcaca tggtcctgct ggagttccgc accgccgccg ggatcactct
     4321 cggcatggac gagctgtaca agggaagcgg agctactaac ttcagcctgc tgaagcaggc
     4381 tggagacgtg gaggagaacc ctggacctaa aaagcctgaa ctcaccgcga cgtctgtcga
     4441 gaagtttctg atcgaaaagt tcgacagcgt ctccgacctg atgcagctct cggagggcga
     4501 agaatctcgt gctttcagct tcgatgtagg agggcgtgga tatgtcctgc gggtaaatag
     4561 ctgcgccgat ggtttctaca aagatcgtta tgtttatcgg cactttgcat cggccgcgct
     4621 cccgattccg gaagtgcttg acattgggga attcagcgag agcctgacct attgcatctc
     4681 ccgccgtgca cagggtgtca cgttgcaaga cctgcctgaa accgaactgc ccgctgttct
     4741 gcagccggtc gcggaggcca tggatgcgat cgctgcggcc gatcttagcc agacgagcgg
     4801 gttcggccca ttcggaccgc aaggaatcgg tcaatacact acatggcgtg atttcatatg
     4861 cgcgattgct gatccccatg tgtatcactg gcaaactgtg atggacgaca ccgtcagtgc
     4921 gtccgtcgcg caggctctcg atgagctgat gctttgggcc gaggactgcc ccgaagtccg
     4981 gcacctcgtg cacgcggatt tcggctccaa caatgtcctg acggacaatg gccgcataac
     5041 agcggtcatt gactggagcg aggcgatgtt cggggattcc caatacgagg tcgccaacat
     5101 cttcttctgg aggccgtggt tggcttgtat ggagcagcag acgcgctact tcgagcggag
     5161 gcatccggag cttgcaggat cgccgcggct ccgggcgtat atgctccgca ttggtcttga
     5221 ccaactctat cagagcttgg ttgacggcaa tttcgatgat gcagcttggg cgcagggtcg
     5281 atgcgacgca atcgtccgat ccggagccgg gactgtcggg cgtacacaaa tcgcccgcag
     5341 aagcgcggcc gtctggaccg atggctgtgt agaagtactc gccgatagtg gaaaccgacg
     5401 ccccagcact cgtccggatc gggagatggg ggaggctaac taagaattcg atatcaagct
     5461 tatcgataat caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta
     5521 tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc
     5581 ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga
     5641 ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac
     5701 ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc
     5761 cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc
     5821 tcggctgttg ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg
     5881 gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc
     5941 ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc
     6001 gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcatcg
     6061 ataccgtcga cctcgagacc tagaaaaaca tggagcaatc acaagtagca atacagcagc
     6121 taccaatgct gattgtgcct ggctagaagc acaagaggag gaggaggtgg gttttccagt
     6181 cacacctcag gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt
     6241 tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac aagatatcct
     6301 tgatctgtgg atctaccaca cacaaggcta cttccctgat tggcagaact acacaccagg
     6361 gccagggatc agatatccac tgacctttgg atggtgctac aagctagtac cagttgagca
     6421 agagaaggta gaagaagcca atgaaggaga gaacacccgc ttgttacacc ctgtgagcct
     6481 gcatgggatg gatgacccgg agagagaagt attagagtgg aggtttgaca gccgcctagc
     6541 atttcatcac atggcccgag agctgcatcc ggactgtact gggtctctct ggttagacca
     6601 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
     6661 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
     6721 atccctcaga cccttttagt cagtgtggaa aatctctagc agggcccgtt taaacccgct
     6781 gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc
     6841 cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg
     6901 catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca
     6961 agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggctt
     7021 ctgaggcgga aagaaccagc tggggctcta gggggtatcc ccacgcgccc tgtagcggcg
     7081 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc
     7141 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc
     7201 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg
     7261 accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg
     7321 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg
     7381 gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt
     7441 cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat taattctgtg
     7501 gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     7561 aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg
     7621 cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc
     7681 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
     7741 tttttttatt tatgcagagg ccgaggccgc ctctgcctct gagctattcc agaagtagtg
     7801 aggaggcttt tttggaggcc taggcttttg caaaaagctc ccgggagctt gtatatccat
     7861 tttcggatct gatcagcacg tgttgacaat taatcatcgg catagtatat cggcatagta
     7921 taatacgaca aggtgaggaa ctaaaccatg gccaagttga ccagtgccgt tccggtgctc
     7981 accgcgcgcg acgtcgccgg agcggtcgag ttctggaccg accggctcgg gttctcccgg
     8041 gacttcgtgg aggacgactt cgccggtgtg gtccgggacg acgtgaccct gttcatcagc
     8101 gcggtccagg accaggtggt gccggacaac accctggcct gggtgtgggt gcgcggcctg
     8161 gacgagctgt acgccgagtg gtcggaggtc gtgtccacga acttccggga cgcctccggg
     8221 ccggccatga ccgagatcgg cgagcagccg tgggggcggg agttcgccct gcgcgacccg
     8281 gccggcaact gcgtgcactt cgtggccgag gagcaggact gacacgtgct acgagatttc
     8341 gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc
     8401 tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc caacttgttt
     8461 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
     8521 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc
     8581 tgtataccgt cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg
     8641 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
     8701 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
     8761 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
     8821 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     8881 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     8941 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     9001 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     9061 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     9121 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     9181 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     9241 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     9301 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     9361 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     9421 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
     9481 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     9541 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     9601 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     9661 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     9721 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     9781 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     9841 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     9901 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
     9961 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
    10021 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
    10081 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
    10141 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
    10201 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
    10261 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
    10321 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
    10381 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
    10441 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
    10501 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
    10561 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
    10621 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
    10681 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
    10741 gttccgcgca catttccccg aaaagtgcca cctgac
//
