LOCUS       Exported                6373 bp ds-DNA     circular SYN 02-AUG-2016
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pALM143_YFP_PcptOO_C38T, A39T_YFP_PMB2_LacIWF (1)
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6373)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Aug 5, 2016 from SnapGene 3.1.4
            http://www.snapgene.com
COMMENT     >pAQ1_YFP
FEATURES             Location/Qualifiers
     source          1..6373
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    545..1450
                     /note="Bla AmpR"
     misc_feature    1630..2249
                     /note="pBR322 ori"
     misc_feature    2422..2621
                     /note="Untitled"
     misc_feature    2686..3224
                     /note="acsA up"
     misc_feature    3202..3224
                     /note="acsA-UpR [EcoRI]"
     misc_feature    3225..3227
                     /note="Flanking Region"
     misc_feature    3228..3251
                     /note="LacOperator"
     gene            3258..3325
                     /note="lacUV5"
     promoter        3258..3325
                     /note="Promoter_P_1"
                     /note="The trc promoter combines the -35 region from the 
                     trp promoter and the -10 region from the lacUV5 promoter"
     misc_feature    3258..3324
                     /note="Promoter"
     -35_signal      3295..3300
                     /note="-35"
     -10_signal      3318..3324
                     /note="-10"
     misc_feature    3325..3359
                     /note="Lac Operator"
     misc_feature    3361..3388
                     /note="195"
     misc_feature    3379..3384
                     /note="RBS"
     misc_feature    3399..3428
                     /note="His Tag"
     misc_feature    3456..4193
                     /note="YFP"
     misc_feature    4267..4285
                     /note="T7 terminator"
     misc_feature    complement(4327..4352)
                     /note="63"
     misc_feature    4423..4457
                     /note="pMB2 Promoter"
     gene            4475..5566
                     /note="LacI"
     misc_feature    5144
                     /note="W220"
     misc_feature    5667..6160
                     /note="acsA down"
ORIGIN
        1 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
       61 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
      121 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
      181 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
      241 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
      301 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct
      361 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa
      421 cgcttacaat ttcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg
      481 catcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
      541 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
      601 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
      661 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
      721 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
      781 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
      841 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
      901 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
      961 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     1021 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     1081 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     1141 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
     1201 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
     1261 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     1321 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     1381 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     1441 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     1501 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     1561 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     1621 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     1681 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     1741 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
     1801 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     1861 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     1921 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     1981 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     2041 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     2101 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     2161 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     2221 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     2281 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     2341 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
     2401 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
     2461 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa
     2521 ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc
     2581 gtatgttgtg tggaattgtg agcggataac aatttcacac aggaacagct atgaccatga
     2641 ttacgccaag ctatttaggt gacactatag aatactcaag ctatgaggtg actgccgcac
     2701 tcaatgttca accagatcta gtgattattt cggggttagc agccgatgcg ggcaacctgg
     2761 tgaagcaact gcgagaatta ggttacaacg gcattattgt tggggggaac ggcttaaata
     2821 cttctaatat tttccccgtc tgccaagcaa aatgtgatgg ggtgttggtg gcccaagcct
     2881 acagtgccga gttagataat gagattaacc gcgcgtttcg ggacgcctat tttcaacaaa
     2941 accaaaaaga gccgccccaa tttagtgccc aggcttttac ggcgatccaa gtttttgttg
     3001 aagccctcag cagcctcgat gaaaaaacgc ccttagaaac tcttgctcta ccggacttgc
     3061 gacgacaact gcgggacgaa atttttgcag gtacctacgt cacgcctttg ggtgaaattt
     3121 ccttcacaga ggaaggggaa attgtccaga aggaattttt tgtggcccaa attgaaatgg
     3181 atgaatcggg tcaacagggg cgtttcgcct tcattgaaac gaacaaacga attgtgagcg
     3241 ctcacaattc ggaattctta acaaaaaagc aggaataaaa ttaacaagat gtaattgaca
     3301 taagtcccat caccgttgta taaatgtgtg gaattgtgag cggataacaa tttcacacac
     3361 caactcataa agtcaagtag gagattaatt ccatgggcca tcatcatcat catcatcatc
     3421 atcatcacag cagcggccat atcgaaggtc gtcatatgca ggcagcagcc tcgatggtga
     3481 gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg
     3541 taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc
     3601 tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga
     3661 ccaccttcgg ctacggcctg cagtgcttcg cccgctaccc cgaccacatg aagcagcacg
     3721 acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg
     3781 acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc
     3841 gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg
     3901 agtacaacta caacagccac aacgtctata tcatggccga caagcagaag aacggcatca
     3961 aggtgaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact
     4021 accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga
     4081 gctaccagtc cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg
     4141 agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag taacgagggc
     4201 ggtgctttgg caggatccgg ctgtctaaca aagcccgaaa ggaagctgag ttggctgctg
     4261 ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgacgggtt
     4321 ttttgtctag atcaacggcc tcaacctact actgggctgc ttcctaatgc aggagtcgca
     4381 taagggagag cgtcgagatc ccggacacca tcgaatggcg catttacagc tagctcagtc
     4441 ctaggtacaa tgctagccgg aggagggtca attcatggtg gtgaatgtga aaccagtaac
     4501 gttatacgat gtcgcagagt atgccggtgt ctcttatcag accgtttccc gcgtggtgaa
     4561 ccaggccagc cacgtttctg cgaaaacgcg ggaaaaagtg gaagcggcga tggcggagct
     4621 gaattacatt cccaaccgcg tggcacaaca actggcgggc aaacagtcgt tgctgattgg
     4681 cgttgccacc tccagtctgg ccctgcacgc gccgtcgcaa attgtcgcgg cgattaaatc
     4741 tcgcgccgat caactgggtg ccagcgtggt ggtgtcgatg gtagaacgaa gcggcgtcga
     4801 agcctgtaaa gcggcggtgc acaatcttct cgcgcaacgc gtcagtgggc tgatcattaa
     4861 ctatccgctg gatgaccagg atgccattgc tgtggaagct gcctgcacta atgttccggc
     4921 gttatttctt gatgtctctg accagacacc catcaacagt attattttct cccatgaaga
     4981 cggtacgcga ctgggcgtgg agcatctggt cgcattgggt caccagcaaa tcgcgctgtt
     5041 agcgggccca ttaagttctg tctcggcgcg tctgcgtctg gctggctggc ataaatatct
     5101 cactcgcaat caaattcagc cgatagcgga acgggaaggc gactttagtg ccatgtccgg
     5161 ttttcaacaa accatgcaaa tgctgaatga gggcatcgtt cccactgcga tgctggttgc
     5221 caacgatcag atggcgctgg gcgcaatgcg cgccattacc gagtccgggc tgcgcgttgg
     5281 tgcggatatc tcggtagtgg gatacgacga taccgaagac agctcatgtt atatcccgcc
     5341 gttaaccacc atcaaacagg attttcgcct gctggggcaa accagcgtgg accgcttgct
     5401 gcaactctct cagggccagg cggtgaaggg caatcagctg ttgcccgtct cactggtgaa
     5461 aagaaaaacc accctggcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
     5521 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa
     5581 ttaatgtaag ttagctcact cattaggcac cgggatctcg accgatgccc ttgagagcct
     5641 tcaacccagt cagctccttc cggttcgcgc tcaagttgat cgaatgtatg ccccggatat
     5701 gacctaggga gattcttcta aagcgagcag ggtttgccaa gtggcgatcg cctcagggaa
     5761 ccataaccaa ctttgttcga ggcggcgggg gttgagctgg gaacgatcga gatctagggc
     5821 gatcgcccgt agggaaagcc gaaaaactcc aagaatttcc tggtcaacgt cggtgggttg
     5881 cctttgggcc aaatcgtaca tactaatcgc caggcccgca taaatctccg cctggagggc
     5941 tggggggaaa tcctgggtcg ttgccgccgc taaggcttca taccaacgat cattggcggc
     6001 ttcgtaatct gcattgaggt aatggacaaa acccagggcc atcaaaattt cggggtcgtt
     6061 gggctgttgg gctagggcca tgtcccaatt gtcttgcacc gtggcgatcg ccgttgcttg
     6121 ggttggcgat ggtgggacgg cagccaaagt tgtttgccag catgcgccct atagtgagtc
     6181 gtattacaat tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac
     6241 ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc
     6301 ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatgga cgcgccctgt
     6361 agcggcgcat taa
//
