LOCUS       pICE-HA-MRE11-WT        7150 bp    DNA     circular
SOURCE      
  ORGANISM  
FEATURES             Location/Qualifiers
     misc_feature    3327..3929
                     /vntifkey="21"
                     /label=PuroR
     rep_origin      complement(4573..5243)
                     /invitrogen="2540000"
                     /vntifkey="33"
                     /label=pUC\origin
     promoter        complement(6249..6347)
                     /invitrogen="2330000"
                     /vntifkey="30"
                     /label=bla\promoter
     CDS             complement(5388..6248)
                     /invitrogen="1020000"
                     /vntifkey="4"
                     /label=Amp(R)
     misc_feature    6424..7148
                     /vntifkey="21"
                     /label=CMVTET
     misc_feature    7010..7049
                     /vntifkey="21"
                     /label=TetO
     polyA_site      2237..2461
                     /invitrogen="1870000"
                     /vntifkey="26"
                     /label=BGH\pA
     rep_origin      2507..2935
                     /invitrogen="2500000"
                     /vntifkey="33"
                     /label=f1\origin
     promoter        2940..3309
                     /invitrogen="2290000"
                     /vntifkey="29"
                     /label=SV40\early\promoter
     polyA_signal    4059..4189
                     /invitrogen="1840000"
                     /vntifkey="25"
                     /label=SV40\pA
     misc_feature    22..51
                     /vntifkey="21"
                     /label=HA
     misc_feature    58..2184
                     /vntifkey="21"
                     /label=MRE11
BASE COUNT     1847 a      1753 c      1770 g      1780 t 
ORIGIN
        1 aagcttgcgg ccgccgccac catgtacccc tacgatgtgc ctgactacgc caccggtatg 
       61 agtactgcag atgcacttga tgatgaaaac acatttaaaa tattagttgc aacagatatt 
      121 catcttggat ttatggagaa agatgcagtc agaggaaatg atacgtttgt aacactcgat 
      181 gaaattttaa gacttgccca ggaaaatgaa gtggatttta ttttgttagg tggtgatctt 
      241 tttcatgaaa ataagccctc aaggaaaaca ttacatacct gcctcgagtt attaagaaaa 
      301 tattgtatgg gtgatcggcc tgtccagttt gaaattctca gtgatcagtc agtcaacttt 
      361 ggttttagta agtttccatg ggtgaactat caagatggca acctcaacat ttcaattcca 
      421 gtgtttagta ttcatggcaa tcatgacgat cccacagggg cagatgcact ttgtgccttg 
      481 gacattttaa gttgtgctgg atttgtaaat cactttggac gttcaatgtc tgtggagaag 
      541 atagacatta gtccggtttt gcttcaaaaa ggaagcacaa agattgcgct atatggttta 
      601 ggatccattc cagatgaaag gctctatcga atgtttgtca ataaaaaagt aacaatgttg 
      661 agaccaaagg aagatgagaa ctcttggttt aacttatttg tgattcatca gaacaggagt 
      721 aaacatggaa gtactaactt cattccagaa caatttttgg atgacttcat tgatcttgtt 
      781 atctggggcc atgaacatga gtgtaaaata gctccaacca aaaatgaaca acagctgttt 
      841 tatatctcac aacctggaag ctcagtggtt acttctcttt ccccaggaga agctgtaaag 
      901 aaacatgttg gtttgctgcg tattaaaggg aggaagatga atatgcataa aattcctctt 
      961 cacacagtgc ggcagttttt catggaggat attgttctag ctaatcatcc agacattttt 
     1021 aacccagata atcctaaagt aacccaagcc atacaaagct tctgtttgga gaagattgaa 
     1081 gaaatgcttg aaaatgctga acgggaacgt ctgggtaatt ctcaccagcc agagaagcct 
     1141 cttgtacgac tgcgagtgga ctatagtgga ggttttgaac ctttcagtgt tcttcgcttt 
     1201 agccagaaat ttgtggatcg ggtagctaat ccaaaagaca ttatccattt tttcaggcat 
     1261 agagaacaaa aggaaaaaac aggagaagag atcaactttg ggaaacttat cacaaagcct 
     1321 tcagaaggaa caactttaag ggtagaagat cttgtaaaac agtactttca aaccgcagag 
     1381 aagaatgtgc agctctcact gctaacagaa agagggatgg gtgaagcagt acaagaattt 
     1441 gtggacaagg aggagaaaga tgccattgag gaattagtga aataccagtt ggaaaaaaca 
     1501 cagcgatttc ttaaagaacg tcacattgat gccctcgaag acaaaatcga tgaggaggta 
     1561 cgtcgtttca gagaaaccag acaaaaaaat actaatgaag aagatgatga agtccgtgag 
     1621 gctatgacca gggccagagc actcagatct cagtcagagg agtctgcttc tgcctttagt 
     1681 gctgatgacc ttatgagtat agatttagca gaacagatgg ctaatgactc tgatgatagc 
     1741 atctcagcag caaccaacaa aggaagaggc cgaggaagag gtcgaagagg tggaagaggg 
     1801 cagaattcag catcgagagg agggtctcaa agaggaagag cagacactgg tctggagact 
     1861 tctacccgta gcaggaactc aaagactgct gtgtcagcat ctagaaatat gtctattata 
     1921 gatgccttta aatctacaag acagcagcct tcccgaaatg tcactactaa gaattattca 
     1981 gaggtgattg aggtagatga atcagatgtg gaagaagaca tttttcctac cacttcaaag 
     2041 acagatcaaa ggtggtccag cacatcatcc agcaaaatca tgtcccagag tcaagtatcg 
     2101 aaaggggttg attttgaatc aagtgaggat gatgatgatg atccttttat gaacactagt 
     2161 tctttaagaa gaaatagaag ataaggtacc ctcgagacgc gtctgcagtc tagataaacc 
     2221 cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc 
     2281 gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa 
     2341 attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac 
     2401 agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg 
     2461 gcttctgagg cggaaagaac cagctggggc tctagggggt atccccacgc gccctgtagc 
     2521 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc 
     2581 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt 
     2641 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac 
     2701 ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag 
     2761 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa 
     2821 actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg 
     2881 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattaattc 
     2941 tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta 
     3001 tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag 
     3061 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa 
     3121 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac 
     3181 taattttttt tatttatgca gaggccgagg ccgcctctgc ctctgagcta ttccagaagt 
     3241 agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctcccggga gcttgtatat 
     3301 ccattttcgg atctgatcag cacgtgacca tgaccgagta caagcccacg gtgcgcctcg 
     3361 ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc gccgactacc 
     3421 ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc gagctgcaag 
     3481 aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg 
     3541 ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga 
     3601 tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa cagatggaag 
     3661 gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct 
     3721 cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg 
     3781 ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac ctccccttct 
     3841 acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct 
     3901 ggtgcatgac ccgcaagccc ggtgcctgac acgtgctacg agatttcgat tccaccgccg 
     3961 ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc 
     4021 agcgcgggga tctcatgctg gagttcttcg cccaccccaa cttgtttatt gcagcttata 
     4081 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc 
     4141 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg ctagccgtcg 
     4201 acctctagct agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat 
     4261 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc 
     4321 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga 
     4381 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt 
     4441 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg 
     4501 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac 
     4561 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg 
     4621 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca 
     4681 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc 
     4741 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc 
     4801 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag 
     4861 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc 
     4921 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca 
     4981 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg 
     5041 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg 
     5101 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct 
     5161 ggtagcggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa 
     5221 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg 
     5281 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga 
     5341 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta 
     5401 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc 
     5461 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg 
     5521 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga 
     5581 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt 
     5641 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt 
     5701 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc 
     5761 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc 
     5821 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca 
     5881 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag 
     5941 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg 
     6001 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa 
     6061 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa 
     6121 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga 
     6181 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga 
     6241 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg 
     6301 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt 
     6361 ccccgaaaag tgccacctga cgtcgacgga tcgggagatc ctgtacgggc cagatatccg 
     6421 cgttgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat 
     6481 agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg 
     6541 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata 
     6601 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta 
     6661 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc 
     6721 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac 
     6781 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga 
     6841 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg 
     6901 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg 
     6961 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctct ccctatcagt 
     7021 gatagagatc tccctatcag tgatagagat cgtcgacgag ctcgtttagt gaaccgtcag 
     7081 atcgcctgga gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc 
     7141 agcctccggc 
//
