LOCUS       pMH-SPX-macroHC         6331 bp    DNA     circular      3-AUG-2016
DEFINITION  Complementary copy of pETM-11.
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|-11353548|
COMMENT     VNTDBDATE|-11353548|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pMH-SPX-macroHC|
COMMENT     VNTAUTHORNAME|Michael Hothorn|
COMMENT     VNTAUTHOREML|michael@hothorn.de|
COMMENT     VNTAUTHORWWW|http://structplantbio.org|
COMMENT     VNTREPLTYPE|Plasmid
COMMENT     VNTEXTCHREPL|Bacteria
FEATURES             Location/Qualifiers
     rep_origin      complement(29..335)
                     /gene="f1 origin"
                     /vntifkey="33"
                     /label=f1\origin
                     /note="Rep_Origin"
     CDS             complement(560..1375)
                     /sequence="ORF_2 rf(2)"
                     /vntifkey="4"
                     /label=kanR
                     /note="kanR"
     rep_origin      complement(1481..2100)
                     /gene="pBR322 origin"
                     /vntifkey="33"
                     /label=pBR322\origin
                     /note="Rep_Origin"
     CDS             complement(3515..4597)
                     /sequence="ORF_1 rf(2)"
                     /vntifkey="4"
                     /label=lacI
                     /note="lacI"
     Promoter        complement(4984..5002)
                     /gene="T7 prom"
                     /vntifkey="21"
                     /label=T7\prom
                     /note="Unknown feature type:Promoter"
     terminator      complement(6203..6331)
                     /gene="T7 term"
                     /vntifkey="43"
                     /label=T7\term
                     /note="Terminator"
     misc_feature    6175..6192
                     /vntifkey="21"
                     /label=6xHis-tag
     CDS             5071..6192
                     /vntifkey="4"
                     /label=Vtc4\178\GAS\macro
BASE COUNT     1566 a      1623 c      1605 g      1537 t 
ORIGIN
        1 tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg 
       61 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc 
      121 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg 
      181 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc 
      241 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt 
      301 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc 
      361 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta 
      421 acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttcag gtggcacttt 
      481 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta 
      541 tccgctcatg aattaattct tagaaaaact catcgagcat caaatgaaac tgcaatttat 
      601 tcatatcagg attatcaata ccatattttt gaaaaagccg tttctgtaat gaaggagaaa 
      661 actcaccgag gcagttccat aggatggcaa gatcctggta tcggtctgcg attccgactc 
      721 gtccaacatc aatacaacct attaatttcc cctcgtcaaa aataaggtta tcaagtgaga 
      781 aatcaccatg agtgacgact gaatccggtg agaatggcaa aagtttatgc atttctttcc 
      841 agacttgttc aacaggccag ccattacgct cgtcatcaaa atcactcgca tcaaccaaac 
      901 cgttattcat tcgtgattgc gcctgagcga gacgaaatac gcgatcgctg ttaaaaggac 
      961 aattacaaac aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca tcaacaatat 
     1021 tttcacctga atcaggatat tcttctaata cctggaatgc tgttttcccg gggatcgcag 
     1081 tggtgagtaa ccatgcatca tcaggagtac ggataaaatg cttgatggtc ggaagaggca 
     1141 taaattccgt cagccagttt agtctgacca tctcatctgt aacatcattg gcaacgctac 
     1201 ctttgccatg tttcagaaac aactctggcg catcgggctt cccatacaat cgatagattg 
     1261 tcgcacctga ttgcccgaca ttatcgcgag cccatttata cccatataaa tcagcatcca 
     1321 tgttggaatt taatcgcggc ctagagcaag acgtttcccg ttgaatatgg ctcataacac 
     1381 cccttgtatt actgtttatg taagcagaca gttttattgt tcatgaccaa aatcccttaa 
     1441 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga 
     1501 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg 
     1561 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc 
     1621 agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag 
     1681 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc 
     1741 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg 
     1801 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac 
     1861 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga 
     1921 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt 
     1981 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag 
     2041 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg 
     2101 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta 
     2161 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc 
     2221 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg 
     2281 tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta 
     2341 caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg 
     2401 ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct 
     2461 gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag 
     2521 gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc 
     2581 gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag 
     2641 aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt 
     2701 ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa 
     2761 acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg 
     2821 ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg 
     2881 tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc 
     2941 tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta 
     3001 cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca 
     3061 gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc 
     3121 ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggggccgc 
     3181 catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa 
     3241 ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc 
     3301 gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac 
     3361 gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca 
     3421 ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgagatc ccggtgccta 
     3481 atgagtgagc taacttacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa 
     3541 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat 
     3601 tgggcgccag ggtggttttt cttttcacca gtgagacggg caacagctga ttgcccttca 
     3661 ccgcctggcc ctgagagagt tgcagcaagc ggtccacgct ggtttgcccc agcaggcgaa 
     3721 aatcctgttt gatggtggtt aacggcggga tataacatga gctgtcttcg gtatcgtcgt 
     3781 atcccactac cgagatatcc gcaccaacgc gcagcccgga ctcggtaatg gcgcgcattg 
     3841 cgcccagcgc catctgatcg ttggcaacca gcatcgcagt gggaacgatg ccctcattca 
     3901 gcatttgcat ggtttgttga aaaccggaca tggcactcca gtcgccttcc cgttccgcta 
     3961 tcggctgaat ttgattgcga gtgagatatt tatgccagcc agccagacgc agacgcgccg 
     4021 agacagaact taatgggccc gctaacagcg cgatttgctg gtgacccaat gcgaccagat 
     4081 gctccacgcc cagtcgcgta ccgtcttcat gggagaaaat aatactgttg atgggtgtct 
     4141 ggtcagagac atcaagaaat aacgccggaa cattagtgca ggcagcttcc acagcaatgg 
     4201 catcctggtc atccagcgga tagttaatga tcagcccact gacgcgttgc gcgagaagat 
     4261 tgtgcaccgc cgctttacag gcttcgacgc cgcttcgttc taccatcgac accaccacgc 
     4321 tggcacccag ttgatcggcg cgagatttaa tcgccgcgac aatttgcgac ggcgcgtgca 
     4381 gggccagact ggaggtggca acgccaatca gcaacgactg tttgcccgcc agttgttgtg 
     4441 ccacgcggtt gggaatgtaa ttcagctccg ccatcgccgc ttccactttt tcccgcgttt 
     4501 tcgcagaaac gtggctggcc tggttcacca cgcgggaaac ggtctgataa gagacaccgg 
     4561 catactctgc gacatcgtat aacgttactg gtttcacatt caccaccctg aattgactct 
     4621 cttccgggcg ctatcatgcc ataccgcgaa aggttttgcg ccattcgatg gtgtccggga 
     4681 tctcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg 
     4741 ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc 
     4801 ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg 
     4861 cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg 
     4921 gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga 
     4981 aattaatacg actcactata ggggaattgt gagcggataa caattcccct ctagaaataa 
     5041 ttttgtttaa ctttaagaag gagatatacc atgaagtttg gtgagcactt gagcaagtct 
     5101 cttattagac agtacagcta ctattatatt agttatgatg atctgaagac tgagctagaa 
     5161 gataacttat ctaagaataa cggtcagtgg acgcaagaat tggaaacaga ttttttagaa 
     5221 tctttggaga tcgagttaga caaggtttac acattttgca aagttaagca tagtgaagtt 
     5281 tttagacgtg taaaagaggt tcaagaacaa gtgcaacata ctgttcgttt gttagactcc 
     5341 aataatcctc ctactcagct agattttgag attctggaag aagaactaag tgatatcatt 
     5401 gccgatgttc acgatctagc aaagttttcg agactaaact acactggttt ccaaaagatt 
     5461 atcaagaaac acgataagaa gacaggtttt atcttgaaac cagttttcca agttagatta 
     5521 gactctaaac catttttcaa ggaaaactat gacgaactag tcgttaaaat ttcccaacta 
     5581 tatgatattg ccagaacttc aggtgcggga tccgacggct tcacagtcct ctccaccaag 
     5641 agcctcttcc ttggccagaa gttgcaagtt gtacaggctg acattgcctc gatcgacagt 
     5701 gatgctgtcg ttcacccgac aaacactgac ttctacatcg gtggtgaagt aggaaacacg 
     5761 ctggagaaga aaggtggcaa ggagtttgtg gaagctgtcc tggaactccg gaaaaagaac 
     5821 gggcccttgg aagtagctgg agctgctgtc agcgcaggcc atggcctgcc tgccaagttt 
     5881 gtgatccact gtaatagtcc agtttggggt gcagacaagt gtgaagaact tctggaaaag 
     5941 acagtgaaaa actgcttggc cctggctgat gataagaagc tgaaatccat tgcatttcca 
     6001 tccatcggca gcggcaggaa cggttttcca aagcagacag cagctcagct gattctgaag 
     6061 gccatctcca gttacttcgt gtctacaatg tcctcttcca tcaaaacggt gtacttcgtg 
     6121 ctttttgaca gcgagagtat aggcatctat gtgcaggaaa tggccaagct cgagcaccac 
     6181 caccaccacc actgagatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct 
     6241 gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt 
     6301 tttttgctga aaggaggaac tatatccgga t 
//
