LOCUS       pVHed05                 7352 bp    DNA     circular UNA 03-AUG-2016
DEFINITION  Bonnet2013_DRC + additional TetR.
ACCESSION   urn.local.vhsiao.2q-6fyl8v0
VERSION     urn.local.vhsiao.2q-6fyl8v0
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     misc_feature    1..22
                     /created_by="User"
                     /label="prefix BBa"
     gene            complement(23..646)
                     /created_by="User"
                     /label="TetR"
     promoter        761..814
                     /created_by="User"
                     /label="pTetO"
     misc_feature    821..845
                     /created_by="User"
                     /label="6N-RBS"
                     /note="Geneious type: ribosome binding site"
     gene            846..2348
                     /created_by="User"
                     /label="Bxb1 Integrase"
     terminator      2369..2463
                     /created_by="User"
                     /label="B0014"
     gene            complement(2486..3363)
                     /created_by="User"
                     /label="AraC"
     promoter        3564..3693
                     /created_by="User"
                     /label="pBad"
     misc_feature    3700..3727
                     /created_by="User"
                     /label="JBEI RBS"
                     /note="Geneious type: ribosome binding site"
     misc_feature    3703..3716
                     /created_by="User"
                     /modified_by="User"
                     /label="Bba_B0031"
                     /note="Geneious type: ribosome binding site"
     gene            3723..5249
                     /created_by="User"
                     /label="TP901 Integrase"
     misc_feature    3729..3746
                     /created_by="User"
                     /label="6x His tag"
     misc_feature    5249..5269
                     /created_by="User"
                     /modified_by="User"
                     /label="Suffix BBa"
     ori             complement(5558..6240)
                     /created_by="User"
                     /modified_by="User"
                     /label="ColE1"
     resistance      6365..7026
                     /created_by="User"
                     /label="Chloramphenicol R"
ORIGIN      
        1 gaattcgcgg ccgcttctag agttaagacc cactttcaca tttaagttgt ttttctaatc
       61 cgcatatgat caattcaagg ccgaataaga aggctggctc tgcaccttgg tgatcaaata
      121 attcgatagc ttgtcgtaat aatggcggca tactatcagt agtaggtgtt tccctttctt
      181 ctttagcgac ttgatgctct tgatcttcca atacgcaacc taaagtaaaa tgccccacag
      241 cgctgagtgc atataatgca ttctctagtg aaaaaccttg ttggcataaa aaggctaatt
      301 gattttcgag agtttcatac tgtttttctg taggccgtgt acctaaatgt acttttgctc
      361 catcgcgatg acttagtaaa gcacatctaa aacttttagc gttattacgt aaaaaatctt
      421 gccagctttc cccttctaaa gggcaaaagt gagtatggtg cctatctaac atctcaatgg
      481 ctaaggcgtc gagcaaagcc cgcttatttt ttacatgcca atacaatgta ggctgctcta
      541 cacctagctt ctgggcgagt ttacgggttg ttaaaccttc gattccgacc tcattaagca
      601 gctctaatgc gctgttaatc actttacttt tatctaatct tgacatcatt aattcctaat
      661 ttttgttgac actctatcgt tgatagagtt attttaccac tccctatcag tgatagagaa
      721 aagtgaaaat ccatatgact agcagatcca ctagagtcga tccctatcag tgatagagat
      781 tgacatccct atcagtgata gagatactga gcacaagctt gcggctttca cacataggag
      841 ctagcgtgag agccctggta gtcatccgcc tgtcccgcgt caccgatgct acgacttcac
      901 cggagcgtca gctggagtct tgccagcagc tctgcgccca gcgcggctgg gacgtcgtcg
      961 gggtagcgga ggatctggac gtctccgggg cggtcgatcc gttcgaccgg aagcgcagac
     1021 cgaacctggc ccggtggcta gcgttcgagg agcaaccgtt tgacgtgatc gtggcgtacc
     1081 gggtagatcg gttgacccga tcgatccggc atcttcagca gctggtccac tgggccgagg
     1141 accacaagaa gctggtcgtc tccgcgaccg aagcgcactt cgatacgacg acgccgtttg
     1201 cggcggtcgt catcgcgctt atgggaacgg tggcgcagat ggaattagaa gcgatcaaag
     1261 agcggaaccg ttcggctgcg catttcaata tccgcgccgg gaaataccga ggatccctgc
     1321 cgccgtgggg atacctgcct acgcgcgtgg acggggagtg gcggctggtg ccggaccctg
     1381 tgcagcgaga gcgcatcctc gaggtgtatc accgcgtcgt cgacaaccac gagccgctgc
     1441 atctggtggc ccacgacctg aaccggcgtg gtgtcctgtc gccgaaggac tacttcgcgc
     1501 agctgcaagg ccgcgagccg cagggccggg agtggtcggc taccgcgctg aagcgatcga
     1561 tgatctccga ggcgatgctc gggtacgcga ctctgaacgg taagaccgtc cgagacgacg
     1621 acggagcccc gctggtgcgg gctgagccga tcctgacccg tgagcagctg gaggcgctgc
     1681 gcgccgagct cgtgaagacc tcccgggcga agcccgcggt gtctaccccg tcgctgctgc
     1741 tgcgggtgtt gttctgcgcg gtgtgcgggg agcccgcgta caagttcgcc gggggaggac
     1801 gtaagcaccc gcgctaccgc tgccgctcga tggggttccc gaagcactgc gggaacggca
     1861 cggtggcgat ggccgagtgg gacgcgttct gcgaggagca ggtactggat ctgctcgggg
     1921 acgcggagcg tctggagaaa gtctgggtag cgggctcgga ctccgcggtc gaactcgcgg
     1981 aggtgaacgc ggagctggtg gacctgacgt cgctgatcgg ctccccggcc taccgggcgg
     2041 gctctccgca gcgagaagca ctggatgccc gtattgcggc gctggccgcg cggcaagagg
     2101 agctggaggg cctggaggct cgcccgtctg gctgggagtg gcgcgagacc gggcagcggt
     2161 tcggggactg gtggcgggag caggacaccg cggcaaagaa cacctggctt cggtcgatga
     2221 acgttcggct gacgttcgac gtccgcggcg ggctgactcg cacgatcgac ttcggggatc
     2281 ttcaggagta cgagcagcat ctcaggctcg gcagcgtggt cgaacggcta cacaccggga
     2341 tgtcgtaata atactagatg cattaataat cacactggct caccttcggg tgggcctttc
     2401 tgcgtttata tactagagag agaatataaa aagccagatt attaatccgg cttttttatt
     2461 atttcctgca gggcggccgc catatgttat gacaacttga cggctacatc attcactttt
     2521 tcttcacaac cggcacggaa ctcgctcggg ctggccccgg tgcatttttt aaatacccgc
     2581 gagaaataga gttgatcgtc aaaaccaaca ttgcgaccga cggtggcgat aggcatccgg
     2641 gtggtgctca aaagcagctt cgcctggctg atacgttggt cctcgcgcca gcttaagacg
     2701 ctaatcccta actgctggcg gaaaagatgt gacagacgcg acggcgacaa gcaaacatgc
     2761 tgtgcgacgc tggcgatatc aaaattgctg tctgccaggt gatcgctgat gtactgacaa
     2821 gcctcgcgta cccgattatc catcggtgga tggagcgact cgttaatcgc ttccatgcgc
     2881 cgcagtaaca attgctcaag cagatttatc gccagcagct ccgaatagcg cccttcccct
     2941 tgcccggcgt taatgatttg cccaaacagg tcgctgaaat gcggctggtg cgcttcatcc
     3001 gggcgaaaga accccgtatt ggcaaatatt gacggccagt taagccattc atgccagtag
     3061 gcgcgcggac gaaagtaaac ccactggtga taccattcgc gagcctccgg atgacgaccg
     3121 tagtgatgaa tctctcctgg cgggaacagc aaaatatcac ccggtcggca aacaaattct
     3181 cgtccctgat ttttcaccac cccctgaccg cgaatggtga gattgagaat ataacctttc
     3241 attcccagcg gtcggtcgat aaaaaaatcg agataaccgt tggcctcaat cggcgttaaa
     3301 cccgccacca gatgggcatt aaacgagtat cccggcagca ggggatcatt ttgcgcttca
     3361 gccatacttt tcatactccc gccattcaga gaagaaacca attgtccata ttgcatcaga
     3421 cattgccgtc actgcgtctt ttactggctc ttctcgctaa ccaaaccggt aaccccgctt
     3481 attaaaagca ttctgtaaca aagcgggacc aaagccatga caaaaacgcg taacaaaagt
     3541 gtctataatc acggcagaaa agtcacattg attatttgca cggcgtcaca ctttgctatg
     3601 ccatagcatt tttatccata agattagcgg atcctacctg acgcttttta tcgcaactct
     3661 ctactgtttc tccataccgt ttttttgggc tagctactag agtcacacag gaaacctact
     3721 agatgaaaca tcatcaccat caccaccagg ccggcactaa gaaagtagca atctatacac
     3781 gagtatccac tactaaccaa gcagaggaag gcttctcaat tgatgagcaa attgaccgtt
     3841 taacaaaata tgctgaagca atggggtggc aagtatctga tacttatact gatgctggtt
     3901 tttcaggggc caaacttgaa cgcccagcaa tgcaaagatt aatcaacgat atcgagaata
     3961 aagcttttga tacagttctt gtatataagc tagaccgcct ttcacgtagt gtaagagata
     4021 ctctttatct tgttaaggat gtgttcacaa aaaataaaat agactttatc tcgcttaatg
     4081 aaagtattga tacttcttct gctatgggta gcttgtttct cactattctt tctgcaatta
     4141 atgagtttga aagagagaat ataaaagaac gcatgactat gggtaaacta gggcgagcga
     4201 aatctggtaa gtctatgatg tggactaaga cagcttttgg gtattaccac aacagaaaga
     4261 caggtatatt agaaattgtt cctttacaag ctacaatagt tgaacaaata ttcactgatt
     4321 atttatcagg aatatcactt acaaaattaa gagataaact caatgaatct ggacacatcg
     4381 gtaaagatat accgtggtct tatcgtaccc taagacaaac acttgataat ccagtttact
     4441 gtggttatat caaatttaag gacagcctat ttgaaggtat gcacaaacca attatccctt
     4501 atgagactta tttaaaagtt caaaaagagc tagaagaaag acaacagcag acttatgaaa
     4561 gaaataacaa ccctagacct ttccaagcta aatatatgct gtcagggatg gcaaggtgcg
     4621 gttactgtgg agcaccttta aaaattgttc ttggccacaa aagaaaagat ggaagccgca
     4681 ctatgaaata tcactgtgca aatagatttc ctcgaaaaac aaaaggaatt acagtatata
     4741 atgacaataa aaagtgtgat tcaggaactt atgatttaag taatttagaa aatactgtta
     4801 ttgacaacct gattggattt caagaaaata atgactcctt attgaaaatt atcaatggca
     4861 acaaccaacc tattcttgat acttcgtcat ttaaaaagca aatttcacag atcgataaaa
     4921 aaatacaaaa gaactctgat ttgtacctaa atgattttat cactatggat gagttgaaag
     4981 atcgtactga ttcccttcag gctgagaaaa agctgcttaa agctaagatt agcgaaaata
     5041 aatttaatga ctctactgat gtttttgagt tagttaaaac tcagttgggc tcaattccga
     5101 ttaatgaact atcatatgat aataaaaaga aaatcgtcaa caaccttgta tcaaaggttg
     5161 atgttactgc tgataatgta gatatcatat ttaaattcca actcgctacc ggtgctgcta
     5221 aggacgaaaa ctacgctctg gctgcttaat actagtagcg gccgctgcag tccggcaaaa
     5281 aaacgggcaa ggtgtcacca ccctgccctt tttctttaaa accgaaaaga ttacttcgcg
     5341 ttatgcaggc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
     5401 tatcagctca ctcaaaggcg gtaatcctag ggcgttcggc tgcggcgagc ggtatcagct
     5461 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     5521 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     5581 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     5641 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     5701 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     5761 gcgctttctc aatgctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     5821 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     5881 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     5941 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     6001 tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     6061 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     6121 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     6181 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     6241 acttgtgctt ggattctcac caataaaaaa cgcccggcgg caaccgagcg ttctgaacaa
     6301 atccagatgg agttctgagg tcattactgg atctatcaac aggagtccaa gcgagctcga
     6361 tatcaaatta cgccccgccc tgccactcat cgcagtactg ttgtaattca ttaagcattc
     6421 tgccgacatg gaagccatca cagacggcat gatgaacctg aatcgccagc ggcatcagca
     6481 ccttgtcgcc ttgcgtataa tatttgccca tggtgaaaac gggggcgaag aagttgtcca
     6541 tattggccac gtttaaatca aaactggtga aactcaccca gggattggct gagacgaaaa
     6601 acatattctc aataaaccct ttagggaaat aggccaggtt ttcaccgtaa cacgccacat
     6661 cttgcgaata tatgtgtaga aactgccgga aatcgtcgtg gtattcactc cagagcgatg
     6721 aaaacgtttc agtttgctca tggaaaacgg tgtaacaagg gtgaacacta tcccatatca
     6781 ccagctcacc gtctttcatt gccatacgaa attccggatg agcattcatc aggcgggcaa
     6841 gaatgtgaat aaaggccgga taaaacttgt gcttattttt ctttacggtc tttaaaaagg
     6901 ccgtaatatc cagctgaacg gtctggttat aggtacattg agcaactgac tgaaatgcct
     6961 caaaatgttc tttacgatgc cattgggata tatcaacggt ggtatatcca gtgatttttt
     7021 tctccatttt agcttcctta gctcctgaaa atctcgataa ctcaaaaaat acgcccggta
     7081 gtgatcttat ttcattatgg tgaaagttgg aacctcttac gtgccgatca acgtctcatt
     7141 ttcgccagat atcgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag
     7201 gcgtatcacg aggccctttc gtcttcacct cgagtgccac ctgacgtcta agaaaccatt
     7261 attatcatga cattaaccta taaaaatagg cgtatcacga ggcagaattt cagataaaaa
     7321 aaatccttag ctttcgctaa ggatgatttc tg
//
