LOCUS       Exported                7697 bp ds-DNA     circular SYN 24-MAR-2016
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pCas49
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7697)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Mar 24, 2016 from SnapGene 3.1.1
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7697
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             complement(1..861)
                     /codon_start=1
                     /note="beta-lactamase"
                     /note="ampR (confers resistance to ampicillin)"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     -10_signal      complement(905..910)
                     /note="bla promoter (counter-clockwise); GAGACA"
     -35_signal      complement(926..931)
                     /note="bla promoter (counter-clockwise); TTCAAA"
     misc_feature    1479..2066
                     /note="glpK_up"
     misc_feature    2074..3251
                     /note="GmR"
     CDS             2458..2991
                     /codon_start=1
                     /note="GmR"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPRFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        3258..3611
                     /note="PccmK2"
                     /note="native promoter from SynPCC7002 ccm operon"
     CDS             3612..5747
                     /codon_start=1
                     /note="RbcL-GFP"
                     /translation="MVQTKSAGFNAGVQDYRLTYYTPDYTPKDTDLLACFRMTPQPGVP
                     PEECAAAVAAESSTGTWTTVWTDGLTDLDRYKGRCYNVEPVPGEDNQYFCFVAYPLDLF
                     EEGSVTNVLTSLVGNVFGFKALRALRLEDIRFPVALIKTYQGPPHGITVERDLLNKYGR
                     PLLGCTIKPKLGLSAKNYGRAVYECLRGGLDFTKDDENINSQPFMRWRDRFLFVQEAIE
                     KSQAETNEVKGHYLNVTAGTCEEMLKRAEFAKEIGTPIIMHDFLTGGFTANTTLAKWCR
                     DNGVLLHIHRAMHAVIDRQKNHGIHFRVLAKCLRLSGGDHLHSGTVVGKLEGDRAATLG
                     FVDLMREDYVEEDRSRGVFFTQDYASLPGTMPVASGGIHVWHMPALVEIFGDDSCLQFG
                     GGTLGHPWGNAPGATANRVALEACVQARNEGRSLAREGNDVLREAGKWSPELAAALDLW
                     KEIKFEFDTVDTLGSMSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIK
                     ANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     CDS             3612..5024
                     /codon_start=1
                     /note="RbcL coding"
                     /translation="MVQTKSAGFNAGVQDYRLTYYTPDYTPKDTDLLACFRMTPQPGVP
                     PEECAAAVAAESSTGTWTTVWTDGLTDLDRYKGRCYNVEPVPGEDNQYFCFVAYPLDLF
                     EEGSVTNVLTSLVGNVFGFKALRALRLEDIRFPVALIKTYQGPPHGITVERDLLNKYGR
                     PLLGCTIKPKLGLSAKNYGRAVYECLRGGLDFTKDDENINSQPFMRWRDRFLFVQEAIE
                     KSQAETNEVKGHYLNVTAGTCEEMLKRAEFAKEIGTPIIMHDFLTGGFTANTTLAKWCR
                     DNGVLLHIHRAMHAVIDRQKNHGIHFRVLAKCLRLSGGDHLHSGTVVGKLEGDRAATLG
                     FVDLMREDYVEEDRSRGVFFTQDYASLPGTMPVASGGIHVWHMPALVEIFGDDSCLQFG
                     GGTLGHPWGNAPGATANRVALEACVQARNEGRSLAREGNDVLREAGKWSPELAAALDLW
                     KEIKFEFDTVDTL"
     CDS             5031..5744
                     /codon_start=1
                     /note="sfGFP coding"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     misc_feature    5802..5820
                     /note="T7 terminator"
     misc_feature    5894..6493
                     /note="glpK_dn"
     rep_origin      complement(6939..7527)
                     /direction=LEFT
                     /note="pMB1 origin of replication"
                     /note="pMB1 origin of replication (counter-clockwise) 
                     (RNAII -35 to RNA/DNA switch point)"
     misc_RNA        complement(6939..7491)
                     /note="RNAII transcript (complementary strand)"
     -35_signal      7345..7350
                     /note="RNAI promoter (clockwise); TTGAAG"
     -10_signal      7367..7372
                     /note="RNAI promoter (clockwise); GCTACA"
     misc_RNA        7381..7488
                     /note="RNAI transcript"
ORIGIN
        1 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
       61 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
      121 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
      181 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
      241 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
      301 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
      361 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
      421 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
      481 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
      541 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
      601 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
      661 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
      721 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
      781 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
      841 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
      901 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
      961 tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac
     1021 attaacctat aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga
     1081 cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga
     1141 tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg
     1201 gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgc ggtgtgaaat
     1261 accgcacaga tgcgtaagga gaaaataccg catcaggcgc cattcgccat tcaggctgcg
     1321 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
     1381 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
     1441 taaaacgacg gccagtgaat tcgagctcgg tacccgggtg aagcgattgg ctatgatcta
     1501 ccaaagctgg cgatttttct gcaaaccgaa ttagaaccac aatttgattt tttaaagccg
     1561 attaccattg ccaacctgaa ggaaatagcg atcgccttta tggaggatgg ccacgaggct
     1621 tgtcgggtct cccattattg tggatcggta ccggatgagc gggcgagctt taatttacgc
     1681 ttgcggcaat atcgccaggc ccagccttgg ctgcgaaatc atctcgatcc tgcaaggggc
     1741 gatcgcctgc accattggtc ggatcaccaa cgcaccattt tctacggcag gcgcaccaat
     1801 cccgacaccc agcagcggct tgtgttagtg gcgcacatgg ccggggctcc gaagaccgtt
     1861 gagattggca aatggctcgc cctggatttg gatcgttggc agttggcgat cgccacaccg
     1921 actttgaaga tcaacaccat ctatgactta gcccaaattc acttgcacaa tggcgaaggt
     1981 tttctgttat ctgaaattcc tccctaaatg atgtcttttg agcctaaaaa cacacttttt
     2041 tgacctaatt taacccattt aaaaaagctc gagcgcatgc ataaaaactg ttgtaattca
     2101 ttaagcattc tgccgacatg gaagccatca caaacggcat gatgaacctg aatcgccagc
     2161 ggcatcagca ccttgtcgcc ttgcgtataa tatttgccca tggacgcaca ccgtggaaac
     2221 ggatgaaggc acgaacccag ttgacataag cctgttcggt tcgtaaactg taatgcaagt
     2281 agcgtatgcg ctcacgcaac tggtccagaa ccttgaccga acgcagcggt ggtaacggcg
     2341 cagtggcggt tttcatggct tgttatgact gtttttttgt acagtctatg cctcgggcat
     2401 ccaagcagca agcgcgttac gccgtgggtc gatgtttgat gttatggagc agcaacgatg
     2461 ttacgcagca gcaacgatgt tacgcagcag ggcagtcgcc ctaaaacaaa gttaggtggc
     2521 tcaagtatgg gcatcattcg cacatgtagg ctcggccctg accaagtcaa atccatgcgg
     2581 gctgctcttg atcttttcgg tcgtgagttc ggagacgtag ccacctactc ccaacatcag
     2641 ccggactccg attacctcgg gaacttgctc cgtagtaaga cattcatcgc gcttgctgcc
     2701 ttcgaccaag aagcggttgt tggcgctctc gcggcttacg ttctgcccag gtttgagcag
     2761 ccgcgtagtg agatctatat ctatgatctc gcagtctccg gcgagcaccg gaggcagggc
     2821 attgccaccg cgctcatcaa tctcctcaag catgaggcca acgcgcttgg tgcttatgtg
     2881 atctacgtgc aagcagatta cggtgacgat cccgcagtgg ctctctatac aaagttgggc
     2941 atacgggaag aagtgatgca ctttgatatc gacccaagta ccgccaccta acaattcgtt
     3001 caagccgaga tcggcttccc ggccgcggag ttgttcggta aattgtcaca acgccgccag
     3061 gtggcacttt tcggggaaat gtgcgcgccc gcgttcctgc tggcgctggg cctgtttctg
     3121 gcgctggact tcccgctgtt ccgtcagcag cttttcgccc acggccttga tgatcgcggc
     3181 ggccttggcc tgcatatccc gattcaacgg ccccagggcg tccagaacgg gcttcaggcg
     3241 ctcccgaagg tggatcctcc tctgaccttt gggcaacttc ttctagaaac ccctgtctat
     3301 ttaaacgatg aagcattgac tctacttaac cttggtatta cgaaatatca ttgcagttta
     3361 tgttgtcaaa ggtgccattg ttgcttatgc tcttcatagc ttgccaaaat caaaattagt
     3421 agcaaacgcg attcctggcg atcgccttgt ccactataaa gcctgtaaag gaccctaacc
     3481 cctgcgcaga ggcaagtggt cggtcttatg ccgcttccgt tatcccaact agaagcagac
     3541 caaactccca gggggacgcc tgttccttag tatttttaag acattaagct tgataagcga
     3601 ggataaatta gatggttcag accaaatctg ctgggtttaa tgccggtgta caggactacc
     3661 gcctgactta ctacaccccc gattacaccc cgaaagatac cgacttactc gcttgtttcc
     3721 ggatgactcc ccaacctgga gtcccccccg aagaatgtgc tgcggctgtt gcggctgaat
     3781 cttctaccgg tacttggacc actgtatgga ccgatggttt aactgacctc gaccgctaca
     3841 agggtcgttg ctacaatgtt gaacccgttc ccggtgaaga caaccaatat ttctgtttcg
     3901 ttgcttaccc cctcgatctg tttgaagaag gttctgtaac caacgttttg acttccttgg
     3961 ttggtaacgt attcggtttt aaagcgctgc gtgccctgcg cctcgaagat atccgcttcc
     4021 ccgttgcgtt aatcaaaact taccaagggc ctccccacgg gatcactgta gagcgtgacc
     4081 tcctcaacaa gtatggtcgt cctctcctcg gttgtacgat taagccgaag ctcggtctgt
     4141 ctgcgaagaa ctacggtcgt gcggtttatg aatgtctccg tggtggtctt gacttcacca
     4201 aagatgacga aaacatcaac tctcagcctt tcatgcgttg gcgcgatcgc ttcctgttcg
     4261 ttcaagaagc tatcgaaaaa tcccaagctg aaaccaacga agttaagggt cactacctta
     4321 acgtcaccgc tggcacttgc gaagaaatgc tcaagcgggc tgaattcgct aaggaaatcg
     4381 gcactcccat catcatgcac gacttcttaa ctggtggttt cactgcgaat actacccttg
     4441 cgaagtggtg tcgtgataac ggcgttctgc tccacatcca ccgggcaatg cacgcggtaa
     4501 tcgaccgtca gaagaaccac ggtattcact tccgcgttct cgctaagtgt ctccgcctct
     4561 ctggtggtga ccacctccac tccggtacgg ttgttggtaa gctcgaaggc gatcgcgccg
     4621 ccaccctcgg tttcgtagac ctgatgcgtg aagactacgt tgaagaagat cgttctcgcg
     4681 gtgtattctt cacccaagac tacgcttctc tccccggcac catgcctgtg gcttccggtg
     4741 gtatccacgt atggcacatg cctgccctcg ttgaaatctt cggcgacgat tcctgcctcc
     4801 agtttggtgg tggtaccctc ggtcacccct ggggtaacgc acctggtgca actgcaaacc
     4861 gtgttgctct ggaagcttgt gttcaagctc gtaacgaagg tcgcagcctg gcccgtgaag
     4921 gtaatgatgt cctccgtgaa gcaggtaagt ggtcgcctga attggcagcc gccctcgacc
     4981 tgtggaagga aatcaagttc gaattcgata ccgttgacac tctcggctcc atgagcaaag
     5041 gagaagaact tttcactgga gttgtcccaa ttcttgttga attagatggt gatgttaatg
     5101 ggcacaaatt ttctgtccgt ggagagggtg aaggtgatgc tacaaacgga aaactcaccc
     5161 ttaaatttat ttgcactact ggaaaactac ctgttccgtg gccaacactt gtcactactc
     5221 tgacctatgg tgttcaatgc ttttcccgtt atccggatca catgaaacgg catgactttt
     5281 tcaagagtgc catgcccgaa ggttatgtac aggaacgcac tatatctttc aaagatgacg
     5341 ggacctacaa gacgcgtgct gaagtcaagt ttgaaggtga tacccttgtt aatcgtatcg
     5401 agttaaaggg tattgatttt aaagaagatg gaaacattct tggacacaaa cttgagtaca
     5461 actttaactc acacaatgta tacatcacgg cagacaaaca aaagaatgga atcaaagcta
     5521 acttcaaaat tcgccacaac gttgaagatg gttccgttca actagcagac cattatcaac
     5581 aaaatactcc aattggcgat ggccctgtcc ttttaccaga caaccattac ctgtcgacac
     5641 aatctgtcct ttcgaaagat cccaacgaaa agcgtgacca catggtcctt cttgagtttg
     5701 taactgctgc tgggattaca catggcatgg atgagctcta caaataagga tccggctgtc
     5761 taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata
     5821 accccttggg gcctctaaac gggtcttgac gggttttttg tctagatcaa cggcctcaac
     5881 ctactacgga tccccccctc ttgcctacag catctccccc aggggagaat tcttcctgtt
     5941 tcaactccct ctaacgtaaa cccattgaat ttaaaaaaga ctttatgact gctttactgc
     6001 tccatgacca acattattcc cttgatcatg aagcctttct ctcaaccctc agcaacacag
     6061 aaaatttact cattattcaa gatctagatg gcgtttgcat ggggttagtc aaagacccct
     6121 taacccgcaa aattgatcct gactatatcc gcgccacacg caagtttaga gaccacttct
     6181 ttgtcctcac caacggtgaa catgaaggca gaaggggagt aaatcgcatc gttgaacggg
     6241 catttcgcaa tgttgaagcc aaagaggaaa caagctattt acctggttta gcagcagggg
     6301 gtgtgcaatg gcagacagat aatggccaaa tttcccatcc cggtgttagc caagcagaac
     6361 tcgatttcct tgccacagtg ccagatttaa ttggtcaaag tttaggacaa ttttttacta
     6421 aatatgttga tatttttccc gctgagcttc aacctgagct gatccatgct tctgttttag
     6481 ataatctcgt ttcctctaga gtcgacctgc aggcatgcaa gcttggcgta atcatggtca
     6541 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
     6601 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
     6661 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
     6721 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
     6781 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     6841 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     6901 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     6961 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     7021 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     7081 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     7141 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     7201 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     7261 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     7321 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
     7381 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     7441 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     7501 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     7561 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     7621 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     7681 taaacttggt ctgacag
//
