LOCUS       pEMS1938        7835 bp    DNA     circular SYN  2-APR-2012
DEFINITION  Ple261 [cloned in pJ248].
ACCESSION   DNA2.0 Id: 77325
SOURCE      Synthetic.
  ORGANISM  Synthetic
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|617878963|
COMMENT     VNTDBDATE|622307986|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pEMS1938|
COMMENT     VNTAUTHORNAME|Demo User|
COMMENT     VNTOAUTHORNAME|UNKNOWN|
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COMMENT                          "Original Accession Number(s): <a href=\"ncbilink:dnarna/DNA2.0\">DNA2.0</a>; <a href=\"ncbilink:dnarna/Id:\">Id:</a>; <a href=\"ncbilink:dnarna/77325\">77325</a>" 
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FEATURES             Location/Qualifiers
     source          1..7835
                     /organism=synthetic
                     /vntifkey="98"
     gene            complement(45..3020)
                     /gene=Ple261
                     /vntifkey="60"
     misc_feature    complement(45..3020)
                     /product=Ple261
                     /vntifkey="21"
                     /label=Ple261
     misc_feature    6454..7257
                     /product=pUC_ori
                     /vntifkey="21"
                     /label=pUC_ori
     misc_feature    complement(7429..7542)
                     /product=rpn txn terminator
                     /vntifkey="21"
                     /label=rpn\txn\terminator
     misc_feature    3050..3224
                     /product=rrnB1 B2 T1 txn terminator
                     /vntifkey="21"
                     /label=rrnB1\B2\T1\txn\terminator
     misc_feature    7733..7758
                     /product=pTF3
                     /vntifkey="21"
                     /label=pTF3
     misc_feature    complement(3133..3149)
                     /product=pTR
                     /vntifkey="21"
                     /label=pTR
     misc_feature    5733..6407
                     /product=GentR
                     /vntifkey="21"
                     /label=GentR
     misc_feature    3406..4254
                     /product=Ampicilin
                     /vntifkey="21"
                     /label=Ampicilin
BASE COUNT     1895 a      2021 c      1977 g      1942 t 
ORIGIN
        1 atgcatttag aataaatttt gtgtcgccct tattcgactc ttttggcgcg ccacccgaag 
       61 gcccgcagaa cccccaaggc cgtgctgcgc ggcgccctgg gcgggccctg gtgcctttgc 
      121 gcccgcgctc ccggctcttg gccccagtcc gtttgccccg cgggtctgtc gcacctcctg 
      181 ggtctgccag ctcctgccgg ggggtaccct ctttgaaggt tcgggggcgg attctgtccc 
      241 ccgggcccgg ccccccggcc cgaagcagcc aatccgtgcg cgggtcatcg tgccgcagcc 
      301 aatcacagag cctctggcag ctgaagttag ggaaacaacg gctcttgagg ggtagctgag 
      361 ggcgcgggac cgctccccgc ccggcagccg cccccagccc cacccgccgt tgtcctagtc 
      421 gcggccgagc gcattctggg ctggcctagg gcgcgcttct tgggccgcct ccctgcgcgt 
      481 cccggccccg tcacttcaga aggcgctcga cccccagtct gcaccaccca gtccactccc 
      541 acatccccga ctgagccggg tggtctcttc cactcgagcc agctccactt ccctgaccaa 
      601 ggtttgcaga agccccccac cccgtcaccg ccgtagaggc cctcgtaggg gggctctcca 
      661 gaaagcccgc cccggggtcc aagccccacc caggcccttt ctcgcactct cactctccag 
      721 ccccgctctg agtcccagca ctgtctctct catcccatgg caaacagaga ggccaggcag 
      781 gcgtgtggag gtcaggccca gggccccagc ctcacccccc atgccactca ctgcctctct 
      841 ggagcctgag tctctggcaa aaagcggtgg cacaggggct ttccccctgc ctggcgaccc 
      901 ccactctccc aagccgcagg ggcttcccag acctctcaat cttcacaggg gctgctcctc 
      961 ttcctgcggt actgcccgca ccccatcctt gggggtccag ttcaggtgac accacttcag 
     1021 gaagttccca gtgacccagc acactgggtg tagcacccag gggcagtggt ggccccaggc 
     1081 ctagcagcct gctctggcct tcagacagga gagacaaagg gacacggagg ggctgtgccc 
     1141 tgccctccca ccagtggcga tggtgtccct ggcaccccag cccccaggcc accctccgga 
     1201 agccaacttg gagtttcctg gccaggagga agagccccca tccttgctgg aggagggagg 
     1261 cgcgggattt ccgggcattc ttctgcacaa tggcccagcc atttctcgaa aggtccactg 
     1321 gtgtggacac tccgggcccc ctgaggctca ggtgggggca cgcggctcca aaacccacag 
     1381 ccccatgggg gcaccagccc ctttctagta gttctttccc cagtctctct cggccccttt 
     1441 gtttccagta aagtttgaac tccaggccct ggactgaggc cactgggtgc ccagttcttt 
     1501 tatctggtct gtgctcagtt ccattggaca gggccctcca tagaaaaagt atggcactga 
     1561 ggctggtcac agatgtggga ggtttgatga ctttctccct cctggggccc ggttcccttc 
     1621 tcccccttca gatggctctg agatggtaaa tgagataagg ccagcgtctg gctcacctag 
     1681 tcagggaatg gagttggagt aagacagggc aattttaaat tttctttata gtttgagctt 
     1741 tctttctcaa atacagctgt tatctttata ataaaacaaa cacaaaaatg acaagaaaat 
     1801 cagtaaaagg ggactattgg ccaacaattc tgtcttccct tcccagtgcc tctgccagtg 
     1861 tactcatcca tccaccagca gctatggccc aggggcgtgc agcctggctc ctcagaatgg 
     1921 cacatggagc tgggctcctc ccaggagcca cattccttcc cacctccccc cagaggcagg 
     1981 catgggcaac tgaatgcaaa caagatgaag aggaaggaca ctgtaaacta aacaattgtt 
     2041 agctgccaga cgcagccaca acctggagtc acagagccct cctggactca gggccaaaag 
     2101 agacctacac aaggtcacat agtgacccag tccttcccag aaccagtagt ctttggagct 
     2161 taaaaacata agcataggta atcttaccat ttaaatctag ttttcctgaa gatgcctgtg 
     2221 tgcctttggc caacaggatc tttgtccaag gtgacaatga agatagtata ccatgagccc 
     2281 atactgcctg cagagtcgtg gctgccaaaa ttaacagctg tggaattatt tgtgaccctg 
     2341 ttaaggataa acatatttgt cccttttcat tttatgatca gcaactgcta aagttcttac 
     2401 ttgtaattgc aatttactgc cctcactgta ttgcttagtt tagaggtgga cacagaaaga 
     2461 atggaacatt tgttttcttt tgggtctgag ctatgaattt gggccgcatt atcatgcagg 
     2521 ccttggcccc tctgtctctg caagattggg ctttcctgtt tttgtcattc tcagcttact 
     2581 tcgcaggcat gctttgtgta gaggagaaaa ataccctcca aagaacctcc caaggaggtt 
     2641 accaggtaca cggttcagcc aagtgggtga ctcaatggga tgcatctctt tgtgtttgtc 
     2701 agggccggga agggtgtgac agcatgtggg gagagactca gttcccagcc aaataacttt 
     2761 accacctgag ctatgaggca gttcctgcac atcaccactc cagcagggtc cagccttgct 
     2821 gcggcagaaa gcgagtgtgt gggaaagcaa atggctccac acgacgcttg cagtgttcag 
     2881 agggatgagt atgaattgtg tgtatctcag ggaccccttt gacccatgaa tacatgtata 
     2941 gagtattctc tattatgtgg gtgaagtgta tctacttaca tacttaagta tttgtgagta 
     3001 aaacgagaaa aaaaacgcgt ccccgagtcg aataagggcg acacccctca attagcccgg 
     3061 gcgaaaggcc cagtctttcg actgagcctt tcgttttatt tgatgcctgg cagttcccta 
     3121 ctctcgcatg gggagtcccc acactaccat cggcgctacg gcgtttcact tctgagttcg 
     3181 gcatggggtc aggtgggacc accgcgctac tgccgccagg caaacaaggg gtgttatgag 
     3241 ccatattcag gtataaatgg gctcgcgata atgttcagaa ttggttaatt ggttgtaaca 
     3301 ctgaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa 
     3361 taaccctgat aaatgcttca ataatattga aaaaggaaga atatgagtat tcaacatttc 
     3421 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa 
     3481 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa 
     3541 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg 
     3601 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa 
     3661 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc 
     3721 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc 
     3781 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta 
     3841 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag 
     3901 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc gatggcaaca 
     3961 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata 
     4021 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc 
     4081 tggtttattg ctgataaatc cggagccggt gagcgtggtt ctcgcggtat catcgcagcg 
     4141 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca 
     4201 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg 
     4261 taaaggagga aaaaaaaatg agccatattc aacgggaaac gtcgaggccg cgattaaatt 
     4321 ccaacatgga tgctgattta tatgggtata aatgggctcg cgataatgtc gggcaatcag 
     4381 gtgcgacaat ctatcgcttg tatgggaagc ccgatgcgcc agagttgttt ctgaaacatg 
     4441 gcaaaggtag cgttgccaat gatgttacag atgagatggt cagactaaac tggctgacgg 
     4501 aatttatgcc acttccgacc atcaagcatt ttatccgtac tcctgatgat gcatggttac 
     4561 tcaccactgc gatccccgga aaaacagcgt tccaggtatt agaagaatat cctgattcag 
     4621 gtgaaaatat tgttgatgcg ctggcagtgt gcacctgttt gtaattgtcc ttttaacagc 
     4681 gatcgcgtat ttcgcctcgc tcaggcgcaa tcacgaatga ataacggttt ggttgatgcg 
     4741 agtgattttg atgacgagcg taatggctgg cctgttgaac aagtctggaa agaaatgcat 
     4801 aaacttttgc cattctcacc ggattcagtc gtcactcatg gtgatttctc acttgataac 
     4861 cttatttttg acgaggggaa attaataggt tgtattgatg ttggacgagt cggaatcgca 
     4921 gaccgatacc aggatcttgc catcctatgg aactgcctcg gtgagttttc tccttcatta 
     4981 cagaaacggc tttttcaaaa atatggtatt gataatcctg atatgaataa attgcagttt 
     5041 catttgatgc tcgatgagtt tttctaaagg aggaaaaaaa aatggagaaa aaaatcactg 
     5101 gatataccac cgttgatata tcccaatggc atcgtaaaga acattttgag gcatttcagt 
     5161 cagttgctca atgtacctat aaccagaccg ttcagctgga tattacggcc tttttaaaga 
     5221 ccgtaaagaa aaataagcac aagttttatc cggcctttat tcacattctt gcccgcctga 
     5281 tgaatgctca tccggagttc cgtatggcaa tgaaagacgg tgagctggtg atatgggata 
     5341 gtgttcaccc ttgttacacc gttttccatg agtgcacacg acgatttccg gcagtttcta 
     5401 cacatatatt cgcaagatgt ggcgtgttac ggtgaaaacc tggcctattt ccctaaaggg 
     5461 tttattgaga atatgttttt cgtcagcgcc aatccctggg tgagtttcac cagttttgat 
     5521 ttaaacgtgg ccaatatgga caacttcttc gcccccgttt tcactatggg caaatattat 
     5581 acgcaaggcg acaaggtgct gatgccgctg gcgattcagg ttcatcatgc cgtctgtgat 
     5641 ggcttccatg tcggcagaat gcttaatgaa ttacaacagt actgcgatga gtggcagggc 
     5701 ggggcgtaaa cgccgaggag gaaaaaaaaa tgcgctcacg caactggtcc agaaccttga 
     5761 ccgaacgcag cggtggtaac ggcgcagtgg cggttttcat ggcttgttat gactgttttt 
     5821 ttgtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg ggtcgatgtt 
     5881 tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa acaaagttag 
     5941 gtggctcaag tatgggcatc attcgcacat gtaggctcgg ccctgaccaa gtcaaatcca 
     6001 tgcgggctgc tcttgatctt ttcggtcgtg agttcggtga cgtagccacc tactcccaac 
     6061 atcagccgga ctccgattac ctcgggaact tgctccgtag taagacattc atcgcgcttg 
     6121 ctgccttcga ccaagaagcg gttgttggcg ctctcgcggc ttacgttctg cccaagtttg 
     6181 agcagccgcg tagtgagatc tatatctatg atctcgcagt ctccggcgag caccggaggc 
     6241 agggcattgc caccgcgctc atcaatctcc tcaagcatga ggccaacgcg cttggtgctt 
     6301 atgtgatcta cgtgcaagca gattacggtg acgatcccgc agtggctctc tatacaaagt 
     6361 tgggcatacg ggaagaagtg atgcactttg atatcgaccc aagtaccgcc acctaagcag 
     6421 agcattacgc tgacttgacg ggacggcgca agctcatgac caaaatccct taacgtgagt 
     6481 tacgcgcgcg tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg 
     6541 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc 
     6601 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag 
     6661 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttagccc accacttcaa 
     6721 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc 
     6781 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc 
     6841 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta 
     6901 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag 
     6961 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct 
     7021 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga 
     7081 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc 
     7141 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt 
     7201 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg 
     7261 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaaggcg agagtaggga 
     7321 actgccaggc atcaaactaa gcagaaggcc cctgacggat ggcctttttg cgtttctaca 
     7381 aactctttct gtgttgtaaa acgacggcca gtcttaagct cgggccccct gggcggttct 
     7441 gataacgagt aatcgttaat ccgcaaataa cgtaaaaacc cgcttcggcg ggttttttta 
     7501 tggggggagt ttagggaaag agcatttgtc agaatattta agggcgcctg tcactttgct 
     7561 tgatatatga gaattattta accttataaa tgagaaaaaa gcaacgcact ttaaataaga 
     7621 tacgttgctt tttcgattga tgaacaccta taattaaact attcatctat tatttatgat 
     7681 tttttgtata tacaatattt ctagtttgtt aaagagaatt aagaaaataa atctcgaaaa 
     7741 taataaaggg aaaatcagtt tttgatatca aaattataca tgtcaacgat aatacaaaat 
     7801 ataatacaaa ctataagatg ttatcagtat ttatt 
//
