LOCUS       pEMS1944        7886 bp    DNA     circular SYN  2-APR-2012
DEFINITION  Ple267 [cloned in pJ248].
ACCESSION   DNA2.0 Id: 77331
SOURCE      Synthetic.
  ORGANISM  Synthetic
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|617879192|
COMMENT     VNTDBDATE|622308059|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pEMS1944|
COMMENT     VNTAUTHORNAME|Demo User|
COMMENT     VNTOAUTHORNAME|UNKNOWN|
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COMMENT                          "Ple267 [cloned in pJ248]." 1) 
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COMMENT                          "Original Accession Number(s): <a href=\"ncbilink:dnarna/DNA2.0\">DNA2.0</a>; <a href=\"ncbilink:dnarna/Id:\">Id:</a>; <a href=\"ncbilink:dnarna/77331\">77331</a>" 
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FEATURES             Location/Qualifiers
     source          1..7886
                     /organism=synthetic
                     /vntifkey="98"
     gene            complement(45..3071)
                     /gene=Ple267
                     /vntifkey="60"
     misc_feature    complement(45..3071)
                     /product=Ple267
                     /vntifkey="21"
                     /label=Ple267
     misc_feature    6505..7308
                     /product=pUC_ori
                     /vntifkey="21"
                     /label=pUC_ori
     misc_feature    complement(7480..7593)
                     /product=rpn txn terminator
                     /vntifkey="21"
                     /label=rpn\txn\terminator
     misc_feature    3101..3275
                     /product=rrnB1 B2 T1 txn terminator
                     /vntifkey="21"
                     /label=rrnB1\B2\T1\txn\terminator
     misc_feature    7784..7809
                     /product=pTF3
                     /vntifkey="21"
                     /label=pTF3
     misc_feature    complement(3184..3200)
                     /product=pTR
                     /vntifkey="21"
                     /label=pTR
     misc_feature    5784..6458
                     /product=GentR
                     /vntifkey="21"
                     /label=GentR
     misc_feature    3457..4305
                     /product=Ampicilin
                     /vntifkey="21"
                     /label=Ampicilin
BASE COUNT     2121 a      1719 c      1847 g      2199 t 
ORIGIN
        1 atgcatttag aataaatttt gtgtcgccct tattcgactc ttttggcgcg ccgagtttga 
       61 gtgtgtgtct gccgcgtaat tgtatccggg acagaaggga cctgtgtttg tgcgctgaat 
      121 caggtgtcgc cgccttcggt ggttcggggg cgtaagggag gcttcggagt tagcgtgttg 
      181 gctaaggagg gctctctggg ttcacagttg atagcaatgc tttaaccgtg tcctccctcg 
      241 ggtcctcagc gcgcctgctc gtccgcagct cccagcgggc ggccagcccc ccccgccctg 
      301 cgcgcggcga gcagagctgg agcgcgccgc tgcccttccg cacttggctc agcggggcac 
      361 gacacgggcc gccacgcgct cagcctagca tcactcgcct ctgactgcga gcgcctccag 
      421 ctccccgggc tggggcatgg atgcagacgc gccttcgtac cacgcgctcg caggctgggc 
      481 aaggggcagc gcgttgggat ccggccagtc gtccgctgag cgcggttggc ttgcaggctg 
      541 ggacttttcg cgagccctct gggcgctcga gttgcgaact ctgacaccgg cacgtggccc 
      601 cgcccactct tccctctttg cgaggcgtgt cccgcgccct ttctgccacc acccacgtag 
      661 tccctgtgct gtagcggaaa gccttaaaaa acgcgagttt ggggatcctg aaaaaaagta 
      721 aatagggttg ctgggctgaa taacatccct cctctcaggc tcctgctgaa agggccattc 
      781 atgatttgcc tggttgagcc tgtggcgact ttattctgac gaatctgcct ttgccagcag 
      841 cataaccttg ccaagttggc gtcttgacca gcaagcaact gtgcccaagg aagcctctct 
      901 gtggtgcctc agacctgaga ggccgagtgc ctttgagcgc ctgaggtaga ttgtgagttt 
      961 catgaaaacc gagaaagtct aagtgtgcat gtgcgtaaga tattttatat atatatatct 
     1021 catatatata tgagatatgt ctatataacg tggactgaat tgtttattgt ttatgtttcg 
     1081 tgtcgagcat aaattccaga agctaagatg cttccatagc acacaaacgt tatgcgttta 
     1141 aacgttggaa atcaaaaatg ccatgtcagg tcattctgtc cttgttccaa agtgagattc 
     1201 agaaataggt tgggattttc tgcccttaga taagaaaacg gaaattatat tttactgtat 
     1261 atgttatcag ttaatgataa attaaaaaac taatatacac tgctgttatt gtaaaatcca 
     1321 tatgctcaag aatgttcatg gatggagaga tcttcaaatg ccatttcaaa aaagaaaatg 
     1381 cacttttctt ctttgcagat taggaatttg aacatattcc ctgtgcaaaa tgcagggaat 
     1441 attttcacag cctttgtacc tgattaccat actctctatt tatttcttat ttaaattgca 
     1501 ctagaaaacc taaagtaaat gaaatgtaac ataataaaga aaagagaatt tagacattta 
     1561 ctttaaaagc tttatttctc aaaggtcaag cttcagtttc aagctactgt ttgatgatgc 
     1621 atatgtaaat tcttggtaaa aacttgatca ttagcaatta aaacaacttt tttcttttat 
     1681 tatataaaaa tgtttaaaat tgtttcacca catccttgtc cttaaaatgg tgtaaactga 
     1741 ccattgtgta aggtttttgt cagatttttg gatagaaaag gagtatatat tatgtgtaga 
     1801 tatgtccatg tttattgttt ctctaatcta tctatttttg tccacattaa atactgatta 
     1861 ttgaatttct cacagctcta cacagtaagg gtgttccata atgtaaaaca agtttattca 
     1921 atcatcatat gaatagttga aatacacttt tggagtttga aatgataaaa gcaagaaatc 
     1981 agtgttgtga acacttgaag aggcttcatg gagacaggtg gtacaattta tacaggttat 
     2041 tttataagtg ggctaatagt tgatgtctat ttttcacaaa agaaccagat gaagtttata 
     2101 acttaatgac ctcatttctg aatccaggtg tgtcataaat agagcatatg atgcaaatta 
     2161 tgttacctat tgagaacaga gtaaacaaat ttatacaaag caggctgttt ccattatctt 
     2221 aacatcactt cttgatttac taagcctata caggcaccaa gctgctttat aacctgcaat 
     2281 cacctggaac cccgtggaac tctgcagttg atgaccctcc ctcttttggc ctttcctttc 
     2341 ccccagtgtg ctgcacttag gagcagaaga tacatttata aaatttcgtt aaaattatta 
     2401 ccacagttat ttcacaattg ttactaaaat acttcttagt gcaagtggaa caaaaggaag 
     2461 gaaggaccaa agccttcaaa atgttcaatg ataagcttaa agctgccatt ctttctgtgc 
     2521 attgtgggga aaaggaggag gagtttgtgc tgagttcctg tgatgactaa tgaaattttt 
     2581 attcttttca ataagagctt taacaattag gctgtgttat aatgaaatgc ccatttgtcc 
     2641 aaagtctgat tcctggtcca taaatacctc tttgtcagag gtacatccct aaaatatgcc 
     2701 tttgtcttga gtggaatgca accagaggcg tttagtgcaa ggagtctcag taaagcagcc 
     2761 tgaaataaac aatggggctt cagaaaagct gaaatgaaaa tgtatatgta tatgtttttc 
     2821 tgtaccatgt gctattcatt acctgactaa aagattatct acaacaatag ggagtaaaat 
     2881 ttcacactta aatatgatca tagaggaatg tttaaagaat ccaaattaat gaaagacatt 
     2941 ttacattctc agtaacagtg taaaggacat ctggaaacta ccttttttgt tgtatatttt 
     3001 taaaggattt tagttcaaaa ctaactaatg attttaaata gtttgtactt tcctagaaat 
     3061 actttacgcg tccccgagtc gaataagggc gacacccctc aattagcccg ggcgaaaggc 
     3121 ccagtctttc gactgagcct ttcgttttat ttgatgcctg gcagttccct actctcgcat 
     3181 ggggagtccc cacactacca tcggcgctac ggcgtttcac ttctgagttc ggcatggggt 
     3241 caggtgggac caccgcgcta ctgccgccag gcaaacaagg ggtgttatga gccatattca 
     3301 ggtataaatg ggctcgcgat aatgttcaga attggttaat tggttgtaac actgacccct 
     3361 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga 
     3421 taaatgcttc aataatattg aaaaaggaag aatatgagta ttcaacattt ccgtgtcgcc 
     3481 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg 
     3541 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc 
     3601 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact 
     3661 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc 
     3721 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag 
     3781 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat 
     3841 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt 
     3901 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa 
     3961 gccataccaa acgacgagcg tgacaccacg atgcctgtag cgatggcaac aacgttgcgc 
     4021 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg 
     4081 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt 
     4141 gctgataaat ccggagccgg tgagcgtggt tctcgcggta tcatcgcagc gctggggcca 
     4201 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat 
     4261 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaaaggagg 
     4321 aaaaaaaaat gagccatatt caacgggaaa cgtcgaggcc gcgattaaat tccaacatgg 
     4381 atgctgattt atatgggtat aaatgggctc gcgataatgt cgggcaatca ggtgcgacaa 
     4441 tctatcgctt gtatgggaag cccgatgcgc cagagttgtt tctgaaacat ggcaaaggta 
     4501 gcgttgccaa tgatgttaca gatgagatgg tcagactaaa ctggctgacg gaatttatgc 
     4561 cacttccgac catcaagcat tttatccgta ctcctgatga tgcatggtta ctcaccactg 
     4621 cgatccccgg aaaaacagcg ttccaggtat tagaagaata tcctgattca ggtgaaaata 
     4681 ttgttgatgc gctggcagtg tgcacctgtt tgtaattgtc cttttaacag cgatcgcgta 
     4741 tttcgcctcg ctcaggcgca atcacgaatg aataacggtt tggttgatgc gagtgatttt 
     4801 gatgacgagc gtaatggctg gcctgttgaa caagtctgga aagaaatgca taaacttttg 
     4861 ccattctcac cggattcagt cgtcactcat ggtgatttct cacttgataa ccttattttt 
     4921 gacgagggga aattaatagg ttgtattgat gttggacgag tcggaatcgc agaccgatac 
     4981 caggatcttg ccatcctatg gaactgcctc ggtgagtttt ctccttcatt acagaaacgg 
     5041 ctttttcaaa aatatggtat tgataatcct gatatgaata aattgcagtt tcatttgatg 
     5101 ctcgatgagt ttttctaaag gaggaaaaaa aaatggagaa aaaaatcact ggatatacca 
     5161 ccgttgatat atcccaatgg catcgtaaag aacattttga ggcatttcag tcagttgctc 
     5221 aatgtaccta taaccagacc gttcagctgg atattacggc ctttttaaag accgtaaaga 
     5281 aaaataagca caagttttat ccggccttta ttcacattct tgcccgcctg atgaatgctc 
     5341 atccggagtt ccgtatggca atgaaagacg gtgagctggt gatatgggat agtgttcacc 
     5401 cttgttacac cgttttccat gagtgcacac gacgatttcc ggcagtttct acacatatat 
     5461 tcgcaagatg tggcgtgtta cggtgaaaac ctggcctatt tccctaaagg gtttattgag 
     5521 aatatgtttt tcgtcagcgc caatccctgg gtgagtttca ccagttttga tttaaacgtg 
     5581 gccaatatgg acaacttctt cgcccccgtt ttcactatgg gcaaatatta tacgcaaggc 
     5641 gacaaggtgc tgatgccgct ggcgattcag gttcatcatg ccgtctgtga tggcttccat 
     5701 gtcggcagaa tgcttaatga attacaacag tactgcgatg agtggcaggg cggggcgtaa 
     5761 acgccgagga ggaaaaaaaa atgcgctcac gcaactggtc cagaaccttg accgaacgca 
     5821 gcggtggtaa cggcgcagtg gcggttttca tggcttgtta tgactgtttt tttgtacagt 
     5881 ctatgcctcg ggcatccaag cagcaagcgc gttacgccgt gggtcgatgt ttgatgttat 
     5941 ggagcagcaa cgatgttacg cagcagggca gtcgccctaa aacaaagtta ggtggctcaa 
     6001 gtatgggcat cattcgcaca tgtaggctcg gccctgacca agtcaaatcc atgcgggctg 
     6061 ctcttgatct tttcggtcgt gagttcggtg acgtagccac ctactcccaa catcagccgg 
     6121 actccgatta cctcgggaac ttgctccgta gtaagacatt catcgcgctt gctgccttcg 
     6181 accaagaagc ggttgttggc gctctcgcgg cttacgttct gcccaagttt gagcagccgc 
     6241 gtagtgagat ctatatctat gatctcgcag tctccggcga gcaccggagg cagggcattg 
     6301 ccaccgcgct catcaatctc ctcaagcatg aggccaacgc gcttggtgct tatgtgatct 
     6361 acgtgcaagc agattacggt gacgatcccg cagtggctct ctatacaaag ttgggcatac 
     6421 gggaagaagt gatgcacttt gatatcgacc caagtaccgc cacctaagca gagcattacg 
     6481 ctgacttgac gggacggcgc aagctcatga ccaaaatccc ttaacgtgag ttacgcgcgc 
     6541 gtcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt 
     6601 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg 
     6661 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca 
     6721 gataccaaat actgttcttc tagtgtagcc gtagttagcc caccacttca agaactctgt 
     6781 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga 
     6841 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc 
     6901 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact 
     6961 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga 
     7021 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg 
     7081 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt 
     7141 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt 
     7201 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga 
     7261 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac 
     7321 gaccgagcgc agcgagtcag tgagcgagga agcggaaggc gagagtaggg aactgccagg 
     7381 catcaaacta agcagaaggc ccctgacgga tggccttttt gcgtttctac aaactctttc 
     7441 tgtgttgtaa aacgacggcc agtcttaagc tcgggccccc tgggcggttc tgataacgag 
     7501 taatcgttaa tccgcaaata acgtaaaaac ccgcttcggc gggttttttt atggggggag 
     7561 tttagggaaa gagcatttgt cagaatattt aagggcgcct gtcactttgc ttgatatatg 
     7621 agaattattt aaccttataa atgagaaaaa agcaacgcac tttaaataag atacgttgct 
     7681 ttttcgattg atgaacacct ataattaaac tattcatcta ttatttatga ttttttgtat 
     7741 atacaatatt tctagtttgt taaagagaat taagaaaata aatctcgaaa ataataaagg 
     7801 gaaaatcagt ttttgatatc aaaattatac atgtcaacga taatacaaaa tataatacaa 
     7861 actataagat gttatcagta tttatt 
//
