LOCUS       pEMS1953        5548 bp    DNA     circular      6-JUL-2016
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|-11271713|
COMMENT     VNTDBDATE|-11313595|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pEMS1953|
COMMENT     VNTAUTHORNAME|Siu Ling Lam|
COMMENT     VNTAUTHORTEL|6048753877|
COMMENT     VNTAUTHOREML|sling@cmmt.ubc.ca|
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FEATURES             Location/Qualifiers
     enhancer        939..2189
                     /vntifkey="9"
                     /label=hs671
     CDS             complement(4491..5348)
                     /vntifkey="4"
                     /label=AmpR
     promoter        2190..3067
                     /vntifkey="29"
                     /label=Hspa1a\(aka\hsp68)
BASE COUNT     1442 a      1352 c      1385 g      1369 t 
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 
      181 accataaaat tgtaaacgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca 
      241 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaatagc 
      301 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg 
      361 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat 
      421 cacccaaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag 
      481 ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga 
      541 agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa 
      601 ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtactatggt tgctttgacg 
      661 tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgccattc 
      721 gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg 
      781 ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc 
      841 ccagtcacga cgttgtaaaa cgacggccag tgaattggag gctacagtca gtggagagga 
      901 ctttcactga ctgactgact gcgtctcaac ctacgcgtgc tgagattgtg tgatgtggag 
      961 aaaaggaaga tatgcagaga tgttagcatg gccacagggt gatggtggtg gtggtgctgg 
     1021 ggcaagtgtg cactactaat aggagaagat gtcaaaaaaa atgaagcata ataatagtgc 
     1081 aatttatcct tgctaaacgt actgcagtgt gctataatta aatatgaaaa cctaaattta 
     1141 tctctatgtt cggttgtcat taatttaaca gggagtgcac atgttctaat ctaatggttg 
     1201 attttttttc tccatgtgca gcatattaaa atgtatatta gcctcaaggt gcataaacac 
     1261 aaaataatag accaaaggtt ttggcctgtg gagaaaaaaa atccatgctc aaaggccgtc 
     1321 aagtagctgt gatgcagggg agacactcac ttagcctcat taagccaaag ctctcttttg 
     1381 cttgtctttt gttctgcatg acagcagcct ctttgttgta taataattca ttccaagttc 
     1441 gttccaagct accgaaactg gtgaactgtg gccattttaa tgagaagtat tggtggtatg 
     1501 cataatctgg aagccacgtg ggccagacaa tcacagagca ctgcagccag caaaatgaga 
     1561 tattgtcgaa atcacaaatt tgtcaagttg tttttggaca gggtattata ataagcacta 
     1621 aaatatgcat cctattactc tgcatctgcc agtcaaagat cactgtaatg aatgcgtgct 
     1681 gcagggtata accaggccgt tttcctaagt aaaacaaacc acagagtaca gaaaagaatt 
     1741 gtgtgggttt tttttttttt ttttggcatt gtttgacttt tttttttttc ctaatccttt 
     1801 tgcaccagga gtcaggttct cctggcatgg tgtccactgc tagcagtgaa tacacaggcc 
     1861 ctgctggtgc cagggagtta acatgaatac attgcttttt taataggtgt gcttaccatc 
     1921 tgagaataca aaacctcaaa ctcaaggctt caattgagtc tactgaagaa ttcctgggag 
     1981 agaaaaggga aaaaggagga gaaaaaaata tctctcccaa tgatcctaag ccttatgttt 
     2041 tatccttttc tacttcaaat ggcttagcct ttgggacttg ttttgttgtt tgtttttctg 
     2101 tttgtttatt tttgcttttt tgtctgtctg ttgatttatt tgctttttag atgaacctaa 
     2161 agctttggga agaaatgggt gagatgctag agctccagga acatccaaac tgagcagccg 
     2221 gggtcccccc caccccccac cccgccccac gcggcaactt tgagcctgtg ctgggacaga 
     2281 gcctctagtt cctaaattag tccatgaggt cagaggcagc actgccattg taacgcgatt 
     2341 ggagaggatc acgtcaccgg acacgccccc aggcatctcc ctgggtctcc taaacttggc 
     2401 ggggagaagt tttagccctt aagttttagc ctttaacccc catattcaga actgtgcgag 
     2461 ttggcgaaac cccacaaatc acaacaaact gtacacaaca ccgagctaga ggtgatcttt 
     2521 cttgtccatt ccacacaggc cttagtaatg cgtcgccata gcaacagtgt cactagtagc 
     2581 accagcactt ccccacaccc tccccctcag gaatccgtac tctccagtga accccagaaa 
     2641 cctctggaga gttctggaca agggcggaac ccacaactcc gattactcaa gggaggcggg 
     2701 gaagctccac cagacgcgaa actgctggaa gattcctggc cccaaggcct cctccggctc 
     2761 gctgattggc ccagcggaga gtgggcgggg ccggtgaaga ctccttaaag gcgcagggcg 
     2821 gcgagcaggt caccagacgc tgacagctac tcagaaccaa atctggttcc atccagagac 
     2881 aagcgaagac aagagaagca gagcgagcgg cgcgttcccg atcctcggcc aggaccagcc 
     2941 ttccccagag catccctgcc gcggagcgca accttcccag gagcatccct gccgcggagc 
     3001 gcaactttcc ccggagcatc cacgccgcgg agcgcagcct tccagaagca gagcgcggcg 
     3061 ccatggcggc gcgccatgac ccacccgatt ttatcgcgga cagtgtgatc tgcgccagtg 
     3121 ccgcgtccca gttcagtcaa cctcttttgg ggtcgtaaga cttggttacg cttccgcaat 
     3181 aggcgatata cgctacgaat ccttccgcgt ccgagagtta ttctctcttg acaactaaac 
     3241 caagcgcagt gccgtagcaa gagacgactg actgactgac tggaaagagg aagggctgga 
     3301 agaggaagga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg 
     3361 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa 
     3421 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac 
     3481 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt 
     3541 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga 
     3601 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca 
     3661 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg 
     3721 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt 
     3781 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc 
     3841 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct 
     3901 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc 
     3961 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta 
     4021 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca 
     4081 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag 
     4141 tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag 
     4201 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt 
     4261 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa 
     4321 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg 
     4381 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga 
     4441 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta 
     4501 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc 
     4561 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg 
     4621 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga 
     4681 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt 
     4741 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt 
     4801 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc 
     4861 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc 
     4921 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca 
     4981 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag 
     5041 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg 
     5101 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa 
     5161 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa 
     5221 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga 
     5281 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga 
     5341 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg 
     5401 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt 
     5461 ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa 
     5521 aataggcgta tcacgaggcc ctttcgtc 
//
