LOCUS       Exported                3760 bp ds-DNA     circular SYN 21-OCT-2016
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    p249
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3760)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Oct 21, 2016 from SnapGene 3.2.1
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3760
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     gene            complement(146..1543)
                     /note="LacZ"
     primer_bind     379..395
                     /note="M13 fwd"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    396..1526
                     /note="MCS"
     misc_feature    complement(431..1480)
                     /note="1kb mouse Foxp3"
     primer_bind     complement(1539..1555)
                     /note="M13 rev"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1563..1579
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1587..1617)
                     /note="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    1632..1653
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding site"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     gene            complement(1926..2540)
                     /note="rep (pMB1)"
     gene            complement(2700..3560)
                     /note="Amp"
     promoter        complement(3561..3665)
                     /gene="bla"
                     /note="AmpR promoter"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt acctcgcgaa
      421 tgcatctaga tctagatatc caatgtcgac gaattcacac caaatatcta tggtccctgg
      481 taaactcaca acccaggcct gacaggtggg agagggttgg ttagcttggt tgagtgcacc
      541 tgacttcctg gaggtgggga cggaagagag ggctctttgg actcacttgg ccaggctcct
      601 ccctcatggt gactgaatga agtagcagat gccaaatagg tcaggcctgg ctccaatccc
      661 ccaaaacacg gggcccaagg ctatgcattt ataacagttg agggcttcta agcctaataa
      721 actcaggtgc agtttcctcc cagctcttag tgggggtgag gggggtgggg gagctccttt
      781 gtaccccctg gcactggggt gtggactcca ctgacctggg aaggaactat tgccatggct
      841 tccccagagg tgcctccgca ctgcaaacag gccgccgtct ggagcctccc gaggagcaga
      901 ccaggccggg agcacactgc cctgagtact ggtggctacg atgcagcaag agctcttgtc
      961 cattgacgcc tgaggggttg ggaggggtgt ggtaatcgag acacttaagc ttgtcatttc
     1021 attgtagcct ttcaaacatt agtttgtatt ttcttattag ttaaacttgt ttttagacaa
     1081 ttttgtacat gtatgtaata catacagacc actttcaccc tatctcctcc ctcatcgctt
     1141 atcttccttc ttctcattcc tttccccctt catccatgtc tatttatttt ggtcttaaaa
     1201 ttattattgc cattattatt attattgaca gggtcttact atgtagccct gactggcctg
     1261 cagctctcag agatgcactt cctctgcctc ctgagtgttt gcctattcat tttcgttttg
     1321 tctgagacag tctcactata tatctctagt tggccttgca ccttctagcc tagttctacc
     1381 tctggagtgc taggattata agtgtgtgcc accacatctg gcttccactt tttattttat
     1441 gtatatatat atatacccgg gattggattg gatcggatcc tatcggatcc cgggcccgtc
     1501 gactgcagag gcctgcatgc aagcttggcg taatcatggt catagctgtt tcctgtgtga
     1561 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc
     1621 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc
     1681 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc
     1741 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     1801 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     1861 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     1921 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     1981 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     2041 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     2101 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     2161 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     2221 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     2281 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     2341 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
     2401 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     2461 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     2521 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     2581 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     2641 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
     2701 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     2761 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     2821 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
     2881 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
     2941 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
     3001 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
     3061 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
     3121 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
     3181 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
     3241 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
     3301 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
     3361 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
     3421 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
     3481 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
     3541 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
     3601 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
     3661 ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca
     3721 ttaacctata aaaataggcg tatcacgagg ccctttcgtc
//
