LOCUS       pdCas9-C-Tet1-sg       10468 bp    DNA     circular     17-DEC-2014
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     VNTDATE|717609990|
COMMENT     VNTDBDATE|717609990|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pdCas9-C-Tet1-sgRNA2.0|
COMMENT     VNTAUTHORNAME|Invitrogen|
COMMENT     VNTAUTHORTEL|(800)-357-3114|
COMMENT     VNTAUTHORFAX|(760)-602-6500|
COMMENT     VNTAUTHOREML|bioinfosales@invitrogen.com|
COMMENT     VNTAUTHORFTP|ftp://ftp.bioinformatics.invitrogen.com|
COMMENT     VNTAUTHORWWW|http://www.invitrogen.com/bioinformatics|
COMMENT     VNTAUTHORAD1|Invitrogen Corporation|
COMMENT     VNTAUTHORAD2|1620 Faraday Avenue|
COMMENT     VNTAUTHORAD3|Carlsbad, CA 92008 U.S.A.|
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
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FEATURES             Location/Qualifiers
     promoter        416..1214
                     /vntifkey="29"
                     /label=CBh
     exon            1290..5390
                     /vntifkey="61"
                     /label=humanized\Cas9\D10A
     polyA_signal    7491..7722
                     /vntifkey="25"
                     /label=BGH\polyA
     misc_feature    7963..8269
                     /vntifkey="21"
                     /label=f1\origin
     misc_feature    8788..9645
                     /vntifkey="21"
                     /label=AmpR
     misc_feature    9796..10463
                     /vntifkey="21"
                     /label=pUC\ori
     promoter        1..249
                     /vntifkey="29"
                     /label=human\U6\promoter
     misc_feature    5391..5423
                     /vntifkey="21"
                     /label=C'NLS
     misc_feature    1227..1256
                     /vntifkey="21"
                     /label=HA\tag
     misc_feature    1257..1289
                     /vntifkey="21"
                     /label=N'NLS
     misc_feature    5424..5462
                     /vntifkey="21"
                     /label=Linker
     misc_feature    5463..7481
                     /vntifkey="21"
                     /label=Tet1\CD
     misc_feature    6005..6024
                     /vntifkey="21"
                     /label=Tet-inset-f
     misc_feature    250..409
                     /vntifkey="21"
                     /label=sgRNA\2.0\scaffold\(2X\MS2)
BASE COUNT     2727 a      2752 c      2798 g      2191 t 
ORIGIN
        1 gagggcctat ttcccatgat tccttcatat ttgcatatac gatacaaggc tgttagagag 
       61 ataattggaa ttaatttgac tgtaaacaca aagatattag tacaaaatac gtgacgtaga 
      121 aagtaataat ttcttgggta gtttgcagtt ttaaaattat gttttaaaat ggactatcat 
      181 atgcttaccg taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga 
      241 cgaaacaccg ggtcttcgag aagacctgtt ttagagctag gccaacatga ggatcaccca 
      301 tgtctgcagg gcctagcaag ttaaaataag gctagtccgt tatcaacttg gccaacatga 
      361 ggatcaccca tgtctgcagg gccaagtggc accgagtcgg tgcttttttg gtacccgtta 
      421 cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt 
      481 caatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa 
      541 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca 
      601 atgacggtaa atggcccgcc tggcattgtg cccagtacat gaccttatgg gactttccta 
      661 cttggcagta catctacgta ttagtcatcg ctattaccat ggtcgaggtg agccccacgt 
      721 tctgcttcac tctccccatc tcccccccct ccccaccccc aattttgtat ttatttattt 
      781 tttaattatt ttgtgcagcg atgggggcgg gggggggggg ggggcgcgcg ccaggcgggg 
      841 cggggcgggg cgaggggcgg ggcggggcga ggcggagagg tgcggcggca gccaatcaga 
      901 gcggcgcgct ccgaaagttt ccttttatgg cgaggcggcg gcggcggcgg ccctataaaa 
      961 agcgaagcgc gcggcgggcg ggagtcgctg cgacgctgcc ttcgccccgt gccccgctcc 
     1021 gccgccgcct cgcgccgccc gccccggctc tgactgaccg cgttactccc acaggtgagc 
     1081 gggcgggacg gcccttctcc tccgggctgt aattagctga gcaagaggta agggtttaag 
     1141 ggatggttgg ttggtggggt attaatgttt aattacctgg agcacctgcc tgaaatcact 
     1201 ttttttcagg ttggaccggt gccaccatgt acccatacga tgttccagat tacgcttcgc 
     1261 cgaagaaaaa gcgcaaggtc gaagcgtccg acaagaagta cagcatcggc ctggccatcg 
     1321 gcaccaactc tgtgggctgg gccgtgatca ccgacgagta caaggtgccc agcaagaaat 
     1381 tcaaggtgct gggcaacacc gaccggcaca gcatcaagaa gaacctgatc ggagccctgc 
     1441 tgttcgacag cggcgaaaca gccgaggcca cccggctgaa gagaaccgcc agaagaagat 
     1501 acaccagacg gaagaaccgg atctgctatc tgcaagagat cttcagcaac gagatggcca 
     1561 aggtggacga cagcttcttc cacagactgg aagagtcctt cctggtggaa gaggataaga 
     1621 agcacgagcg gcaccccatc ttcggcaaca tcgtggacga ggtggcctac cacgagaagt 
     1681 accccaccat ctaccacctg agaaagaaac tggtggacag caccgacaag gccgacctgc 
     1741 ggctgatcta tctggccctg gcccacatga tcaagttccg gggccacttc ctgatcgagg 
     1801 gcgacctgaa ccccgacaac agcgacgtgg acaagctgtt catccagctg gtgcagacct 
     1861 acaaccagct gttcgaggaa aaccccatca acgccagcgg cgtggacgcc aaggccatcc 
     1921 tgtctgccag actgagcaag agcagacggc tggaaaatct gatcgcccag ctgcccggcg 
     1981 agaagaagaa tggcctgttc ggcaacctga ttgccctgag cctgggcctg acccccaact 
     2041 tcaagagcaa cttcgacctg gccgaggatg ccaaactgca gctgagcaag gacacctacg 
     2101 acgacgacct ggacaacctg ctggcccaga tcggcgacca gtacgccgac ctgtttctgg 
     2161 ccgccaagaa cctgtccgac gccatcctgc tgagcgacat cctgagagtg aacaccgaga 
     2221 tcaccaaggc ccccctgagc gcctctatga tcaagagata cgacgagcac caccaggacc 
     2281 tgaccctgct gaaagctctc gtgcggcagc agctgcctga gaagtacaaa gagattttct 
     2341 tcgaccagag caagaacggc tacgccggct acattgacgg cggagccagc caggaagagt 
     2401 tctacaagtt catcaagccc atcctggaaa agatggacgg caccgaggaa ctgctcgtga 
     2461 agctgaacag agaggacctg ctgcggaagc agcggacctt cgacaacggc agcatccccc 
     2521 accagatcca cctgggagag ctgcacgcca ttctgcggcg gcaggaagat ttttacccat 
     2581 tcctgaagga caaccgggaa aagatcgaga agatcctgac cttccgcatc ccctactacg 
     2641 tgggccctct ggccagggga aacagcagat tcgcctggat gaccagaaag agcgaggaaa 
     2701 ccatcacccc ctggaacttc gaggaagtgg tggacaaggg cgcttccgcc cagagcttca 
     2761 tcgagcggat gaccaacttc gataagaacc tgcccaacga gaaggtgctg cccaagcaca 
     2821 gcctgctgta cgagtacttc accgtgtata acgagctgac caaagtgaaa tacgtgaccg 
     2881 agggaatgag aaagcccgcc ttcctgagcg gcgagcagaa aaaggccatc gtggacctgc 
     2941 tgttcaagac caaccggaaa gtgaccgtga agcagctgaa agaggactac ttcaagaaaa 
     3001 tcgagtgctt cgactccgtg gaaatctccg gcgtggaaga tcggttcaac gcctccctgg 
     3061 gcacatacca cgatctgctg aaaattatca aggacaagga cttcctggac aatgaggaaa 
     3121 acgaggacat tctggaagat atcgtgctga ccctgacact gtttgaggac agagagatga 
     3181 tcgaggaacg gctgaaaacc tatgcccacc tgttcgacga caaagtgatg aagcagctga 
     3241 agcggcggag atacaccggc tggggcaggc tgagccggaa gctgatcaac ggcatccggg 
     3301 acaagcagtc cggcaagaca atcctggatt tcctgaagtc cgacggcttc gccaacagaa 
     3361 acttcatgca gctgatccac gacgacagcc tgacctttaa agaggacatc cagaaagccc 
     3421 aggtgtccgg ccagggcgat agcctgcacg agcacattgc caatctggcc ggcagccccg 
     3481 ccattaagaa gggcatcctg cagacagtga aggtggtgga cgagctcgtg aaagtgatgg 
     3541 gccggcacaa gcccgagaac atcgtgatcg aaatggccag agagaaccag accacccaga 
     3601 agggacagaa gaacagccgc gagagaatga agcggatcga agagggcatc aaagagctgg 
     3661 gcagccagat cctgaaagaa caccccgtgg aaaacaccca gctgcagaac gagaagctgt 
     3721 acctgtacta cctgcagaat gggcgggata tgtacgtgga ccaggaactg gacatcaacc 
     3781 ggctgtccga ctacgatgtg gacgctatcg tgcctcagag ctttctgaag gacgactcca 
     3841 tcgacaacaa ggtgctgacc agaagcgaca agaaccgggg caagagcgac aacgtgccct 
     3901 ccgaagaggt cgtgaagaag atgaagaact actggcggca gctgctgaac gccaagctga 
     3961 ttacccagag aaagttcgac aatctgacca aggccgagag aggcggcctg agcgaactgg 
     4021 ataaggccgg cttcatcaag agacagctgg tggaaacccg gcagatcaca aagcacgtgg 
     4081 cacagatcct ggactcccgg atgaacacta agtacgacga gaatgacaag ctgatccggg 
     4141 aagtgaaagt gatcaccctg aagtccaagc tggtgtccga tttccggaag gatttccagt 
     4201 tttacaaagt gcgcgagatc aacaactacc accacgccca cgacgcctac ctgaacgccg 
     4261 tcgtgggaac cgccctgatc aaaaagtacc ctaagctgga aagcgagttc gtgtacggcg 
     4321 actacaaggt gtacgacgtg cggaagatga tcgccaagag cgagcaggaa atcggcaagg 
     4381 ctaccgccaa gtacttcttc tacagcaaca tcatgaactt tttcaagacc gagattaccc 
     4441 tggccaacgg cgagatccgg aagcggcctc tgatcgagac aaacggcgaa accggggaga 
     4501 tcgtgtggga taagggccgg gattttgcca ccgtgcggaa agtgctgagc atgccccaag 
     4561 tgaatatcgt gaaaaagacc gaggtgcaga caggcggctt cagcaaagag tctatcctgc 
     4621 ccaagaggaa cagcgataag ctgatcgcca gaaagaagga ctgggaccct aagaagtacg 
     4681 gcggcttcga cagccccacc gtggcctatt ctgtgctggt ggtggccaaa gtggaaaagg 
     4741 gcaagtccaa gaaactgaag agtgtgaaag agctgctggg gatcaccatc atggaaagaa 
     4801 gcagcttcga gaagaatccc atcgactttc tggaagccaa gggctacaaa gaagtgaaaa 
     4861 aggacctgat catcaagctg cctaagtact ccctgttcga gctggaaaac ggccggaaga 
     4921 gaatgctggc ctctgccggc gaactgcaga agggaaacga actggccctg ccctccaaat 
     4981 atgtgaactt cctgtacctg gccagccact atgagaagct gaagggctcc cccgaggata 
     5041 atgagcagaa acagctgttt gtggaacagc acaagcacta cctggacgag atcatcgagc 
     5101 agatcagcga gttctccaag agagtgatcc tggccgacgc taatctggac aaagtgctgt 
     5161 ccgcctacaa caagcaccgg gataagccca tcagagagca ggccgagaat atcatccacc 
     5221 tgtttaccct gaccaatctg ggagcccctg ccgccttcaa gtactttgac accaccatcg 
     5281 accggaagag gtacaccagc accaaagagg tgctggacgc caccctgatc caccagagca 
     5341 tcaccggcct gtacgagaca cggatcgacc tgtctcagct gggaggcgac agccccaaga 
     5401 agaagagaaa ggtggaggcc agcggtggag gaagtggagg tggttcaggt ggaggatccg 
     5461 gcgaagctgc accctgtgac tgtgatggag gtacacaaaa agaaaaaggc ccatattata 
     5521 cacaccttgg ggcaggacca agtgtggctg ctgtcaggga gctcatggag actaggtttg 
     5581 gccagaaggg gaaggcaatc cggattgaga agatagtgtt cacggggaag gaagggaaga 
     5641 gctctcaggg ctgcccggtc gccaagtggg tgatcagaag aagtggtcct gaagagaagc 
     5701 ttatttgttt ggttcgtgag cgtgtagacc atcactgttc gacggctgtg atagttgtcc 
     5761 ttatcctgct gtgggaaggt atccctcgcc tgatggctga ccgcctgtac aaagagctca 
     5821 ctgagaactt gaggtcctac agcggacatc ccacagaccg aagatgtacc ctcaacaaaa 
     5881 agcgtacctg cacctgtcaa ggcatcgacc caaaaacctg cggagcgtcc ttctcctttg 
     5941 gctgttcgtg gagcatgtat ttcaacggct gtaagtttgg gaggagtgaa aaccccagaa 
     6001 aattcagact tgctccaaac taccccttac ataactacta taagagaatt actggaatga 
     6061 gttctgaagg aagtgacgtg aaaaccgggt ggatcattcc agaccgcaag accctcataa 
     6121 gcagagagga aaaacagctt gaaaagaatt tacaagaatt ggctacagta ttagctccac 
     6181 tttacaagca gatggctcca gttgcttatc aaaatcaggt ggaatatgaa gaagttgctg 
     6241 gagactgtcg acttggaaat gaagaggggc gtcctttctc tggtgtcacc tgttgcatgg 
     6301 atttttgtgc ccattctcac aaggacattc acaacatgca caacggaagc accgtggtgt 
     6361 gtacgttgat tcgagcagat ggccgtgaca caaattgtcc cgaggatgaa caactccacg 
     6421 tcctgccact ataccggctt gcagacactg atgaatttgg ctccgtggaa gggatgaagg 
     6481 ccaaaatcaa atctggggcc atccaagtca atgggccaac caggaagagg cgactacgtt 
     6541 ttactgagcc tgttcctcga tgtgggaaga gggccaaaat gaagcagaac cacaataaat 
     6601 caggttcaca caacactaag agcttttcat cagcctcatc tacttctcac ctagtgaaag 
     6661 acgaatctac agacttctgt cccctgcagg cttcctccgc agaaacatct acctgtacgt 
     6721 acagtaaaac agcctcaggt gggtttgcag aaacaagtag tattctccac tgcacaatgc 
     6781 cttctggagc acacagtggt gctaatgcag ctgctgggga atgtactgga acggtgcagc 
     6841 ctgccgaggt ggctgctcat cctcaccagt ctcttcccac agccgattct cccgttcatg 
     6901 ctgagcctct cactagtcca tctgagcagc taacttctaa ccagtcaaac cagcagctcc 
     6961 ctctcctcag caattctcag aaactggctt cctgtcaggt ggaagatgag cggcaccctg 
     7021 aagcggatga gcctcagcac cccgaggacg ataacttgcc tcaacttgat gagttctggt 
     7081 cagacagtga ggagatctac gccgatcctt cctttggtgg cgtggcgata gcacccattc 
     7141 acggctcggt gctcattgag tgcgctcgga aggagcttca tgctaccacc tctttgcgct 
     7201 cccccaaacg aggggtccct tttcgtgtgt cccttgtatt ctaccagcac aaaagcctaa 
     7261 acaagcctaa tcatggtttt gatatcaaca aaattaagtg taaatgcaaa aaagtaacga 
     7321 aaaaaaagcc cgcagaccgg gagtgtcctg atgtatcccc cgaagccaat ttatcacacc 
     7381 aaattccttc tcgagttgca tcaaccttaa cccgagacaa tgttgttacc gtgtccccat 
     7441 actctctcac tcatgttgcg ggaccctaca atcgttgggt ctaagaattc ctagagctcg 
     7501 ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt 
     7561 gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat 
     7621 tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag 
     7681 caagggggag gattgggaag agaatagcag gcatgctggg gagcggccgc aggaacccct 
     7741 agtgatggag ttggccactc cctctctgcg cgctcgctcg ctcactgagg ccgggcgacc 
     7801 aaaggtcgcc cgacgcccgg gctttgcccg ggcggcctca gtgagcgagc gagcgcgcag 
     7861 ctgcctgcag gggcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca 
     7921 ccgcatacgt caaagcaacc atagtacgcg ccctgtagcg gcgcattaag cgcggcgggt 
     7981 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc 
     8041 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg 
     8101 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat 
     8161 ttgggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg 
     8221 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct 
     8281 atctcgggct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa 
     8341 aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac gtttacaatt 
     8401 ttatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gccccgacac 
     8461 ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga 
     8521 caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa 
     8581 cgcgcgagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata 
     8641 atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt 
     8701 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg 
     8761 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt 
     8821 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta 
     8881 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc 
     8941 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa 
     9001 gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc 
     9061 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt 
     9121 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact 
     9181 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac 
     9241 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata 
     9301 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta 
     9361 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg 
     9421 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat 
     9481 aaatctggag ccggtgagcg tggaagccgc ggtatcattg cagcactggg gccagatggt 
     9541 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga 
     9601 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa 
     9661 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag 
     9721 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac 
     9781 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc 
     9841 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat 
     9901 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat 
     9961 actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct 
    10021 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt 
    10081 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg 
    10141 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta 
    10201 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg 
    10261 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg 
    10321 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc 
    10381 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg 
    10441 gccttttgct ggccttttgc tcacatgt 
//
