LOCUS       haENaC_flag_pcDNA3      7608 bp            circular circular 12-SEP-2016
DEFINITION  t  12/2016.
ACCESSION   this is my plasmid that Ken Volk reconstructed
VERSION     .  
KEYWORDS    .
SOURCE      .
  ORGANISM  .
FEATURES             Location/Qualifiers
     promoter        209..863
                     /note="CMV"
     promoter        864..882
                     /note="T7"
     misc_feature    944..1066
                     /note="exon 1B"
     misc_feature    944
                     /note="translation start for aenac-2"
     CDS             950..1122
                     /codon_start=1
                     /product="Na+ channel aenac-"
     misc_feature    1067
                     /note="start of exon 2"
     misc_feature    1121
                     /note="translation start for aenac-1"
     CDS             1302..3284
                     /note="hEnaC alpha-1"
     misc_feature    3106..3129
                     /note="flag"
     promoter        3161..3178
                     /note="SP6"
     polyA_signal    3180..3411
                     /note="BGH polyA"
     promoter        3952..4277
                     /note="SV40"
     rep_origin      4146
                     /note="SV40 ori"
     gene            4313..5107
                     /note="Neo"
     polyA_signal    5162..5534
                     /note="SV40 polyA"
     rep_origin      5794
                     /note="ColE1"
     gene            6612..7472
                     /note="Amp"
BASE COUNT     1709 a   2109 c   1968 g   1822 t
ORIGIN
        1 GACGGATCGG GAGATCTCCC GATCCCCTAT GGTCGACTCT CAGTACAATC TGCTCTGATG 
       61 CCGCATAGTT AAGCCAGTAT CTGCTCCCTG CTTGTGTGTT GGAGGTCGCT GAGTAGTGCG 
      121 CGAGCAAAAT TTAAGCTACA ACAAGGCAAG GCTTGACCGA CAATTGCATG AAGAATCTGC 
      181 TTAGGGTTAG GCGTTTTGCG CTGCTTCGCG ATGTACGGGC CAGATATACG CGTTGACATT 
      241 GATTATTGAC TAGTTATTAA TAGTAATCAA TTACGGGGTC ATTAGTTCAT AGCCCATATA 
      301 TGGAGTTCCG CGTTACATAA CTTACGGTAA ATGGCCCGCC TGGCTGACCG CCCAACGACC 
      361 CCCGCCCATT GACGTCAATA ATGACGTATG TTCCCATAGT AACGCCAATA GGGACTTTCC 
      421 ATTGACGTCA ATGGGTGGAC TATTTACGGT AAACTGCCCA CTTGGCAGTA CATCAAGTGT 
      481 ATCATATGCC AAGTACGCCC CCTATTGACG TCAATGACGG TAAATGGCCC GCCTGGCATT 
      541 ATGCCCAGTA CATGACCTTA TGGGACTTTC CTACTTGGCA GTACATCTAC GTATTAGTCA 
      601 TCGCTATTAC CATGGTGATG CGGTTTTGGC AGTACATCAA TGGGCGTGGA TAGCGGTTTG 
      661 ACTCACGGGG ATTTCCAAGT CTCCACCCCA TTGACGTCAA TGGGAGTTTG TTTTGGCACC 
      721 AAAATCAACG GGACTTTCCA AAATGTCGTA ACAACTCCGC CCCATTGACG CAAATGGGCG 
      781 GTAGGCGTGT ACGGTGGGAG GTCTATATAA GCAGAGCTCT CTGGCTAACT AGAGAACCCA 
      841 CTGCTTACTG GCTTATCGAA ATTAATACGA CTCACTATAG GGAGACCCAA GCTTGGTACC 
      901 GAGCTCGGAT CCACTAGTAA CGGCCGCCAG TGTGCTGGAA TTCATGGGCA TGGCCAGGGG 
      961 CAGCCTCACT CGGGTTCCAG GGGTGATGGG AGAGGGCACT CAGGGCCCAG AGCTCAGCCT 
     1021 TGACCCTGAC CCTTGCTCTC CCCAATCCAC TCCGGGGCTC ATGAAGGGGA ACAAGCTGGA 
     1081 GGAGCAGGAC CCTAGACCTC TGCAGCCCAT ACCAGGTCTC ATATGGAGGG GAACAAGCTG 
     1141 GAGGAGCAGG ACTCTAGCCC TCCACAGTCC ACTCCAGGGC TCATGAAGGG GAACAAGCGT 
     1201 GAGGAGCAGG GGCTGGGCCC CGAACCTGCG GCGCCCCAGC AGCCCACGGC GGAGGAGGAG 
     1261 GCCCTGATCG AGTTCCACCG CTCCTACCGA GAGCTCTTCG AGTTCTTCTG CAACAACACC 
     1321 ACCATCCACG GCGCCATCCG CCTGGTGTGC TCCCAGCACA ACCGCATGAA GACGGCCTTC 
     1381 TGGGCAGTGC TGTGGCTCTG CACCTTTGGC ATGATGTACT GGCAATTCGG CCTGCTTTTC 
     1441 GGAGAGTACT TCAGCTACCC CGTCAGCCTC AACATCAACC TCAACTCGGA CAAGCTCGTC 
     1501 TTCCCCGCAG TGACCATCTG CACCCTCAAT CCCTACAGGT ACCCGGAAAT TAAAGAGGAG 
     1561 CTGGAGGAGC TGGACCGCAT CACAGAGCAG ACGCTCTTTG ACCTGTACAA ATACAGCTCC 
     1621 TTCACCACTC TCGTGGCCGG CTCCCGCAGC CGTCGCGACC TGCGGGGGAC TCTGCCGCAC 
     1681 CCCTTGCAGC GCCTGAGGGT CCCGCCCCCG CCTCACGGGG CCCGTCGAGC CCGTAGCGTG 
     1741 GCCTCCAGCT TGCGGGACAA CAACCCCCAG GTGGACTGGA AGGACTGGAA GATCGGCTTC 
     1801 CAGCTGTGCA ACCAGAACAA ATCGGACTGC TTCTACCAGA CATACTCATC AGGGGTGGAT 
     1861 GCGGTGAGGG AGTGGTACCG CTTCCACTAC ATCAACATCC TGTCGAGGCT GCCAGAGACT 
     1921 CTGCCATCCC TGGAGGAGGA CACGCTGGGC AACTTCATCT TCGCCTGCCG CTTCAACCAG 
     1981 GTCTCCTGCA ACCAGGCGAA TTACTCTCAC TTCCACCACC CGATGTATGG AAACTGCTAT 
     2041 ACTTTCAATG ACAAGAACAA CTCCAACCTC TGGATGTCTT CCATGCCTGG AATTAACAAC 
     2101 GGTCTGTCCC TGATGCTGCG CGCAGAGCAG AATGACTTCA TTCCCCTCCT GTCCACAGTG 
     2161 ACTGGGGCCC GGGTAATGGT GCACGGGCAG GATGAACCTG CCTTTATGGA TGATGGTGGC 
     2221 TTTAACTTGC GGCCTGGCGT GGAGACCTCC ATCAGCATGA GGAAGGAAAC CCTGGACAGA 
     2281 CTTGGGGGCG ATTATGGCGA CTGCACCAAG AATGGCAGTG ATGTTCCTGT TGAGAACCTT 
     2341 TACCCTTCCA AGTACACACA GCAGGTGTGT ATTCACTCCT GCTTCCAGGA GAGCATGATC 
     2401 AAGGAGTGTG GCTGTGCCTA CATCTTCTAT CCGCGGCCCC AGAACGTGGA GTACTGTGAC 
     2461 TACAGAAAGC ACAGTTCCTG GGGGTACTGC TACTATAAGC TCCAGGTTGA CTTCTCCTCA 
     2521 GACCACCTGG GCTGTTTCAC CAAGTGCCGG AAGCCATGCA GCGTGACCAG CTACCAGCTC 
     2581 TCTGCTGGTT ACTCACGATG GCCCTCGGTG ACATCCCAGG AATGGGTCTT CCAGATGCTA 
     2641 TCGCGACAGA ACAATTACAC CGTCAACAAC AAGAGAAATG GAGTGGCCAA AGTCAACATC 
     2701 TTCTTCAAGG AGCTGAACTA CAAAACCAAT TCTGAGTCTC CCTCTGTCAC GATGGTCACC 
     2761 CTCCTGTCCA ACCTGGGCAG CCAGTGGAGC CTGTGGTTCG GCTCCTCGGT GTTGTCTGTG 
     2821 GTGGAGATGG CTGAGCTCGT CTTTGACCTG CTGGTCATCA TGTTCCTCAT GCTGCTCCGA 
     2881 AGGTTCCGAA GCCGATACTG GTCTCCAGGC CGAGGGGGCA GGGGTGCTCA GGAGGTAGCC 
     2941 TCCACCCTGG CATCCTCCCC TCCTTCCCAC TTCTGCCCCC ACCCCATGTC TCTGTCCTTG 
     3001 TCCCAGCCAG GCCCTGCTCC CTCTCCAGCC TTGACAGCCC CTCCCCCTGC CTATGCCACC 
     3061 CTGGGCCCCC GCCCATCTCC AGGGGGCTCT GCAGGGGCCA GTTCCGACTA CAAGGACGAC 
     3121 GATGACAAGT GACTCGAGCA TGCATCTAGA GGGCCCTATT CTATAGTGTC ACCTAAATGC 
     3181 TAGAGCTCGC TGATCAGCCT CGACTGTGCC TTCTAGTTGC CAGCCATCTG TTGTTTGCCC 
     3241 CTCCCCCGTG CCTTCCTTGA CCCTGGAAGG TGCCACTCCC ACTGTCCTTT CCTAATAAAA 
     3301 TGAGGAAATT GCATCGCATT GTCTGAGTAG GTGTCATTCT ATTCTGGGGG GTGGGGTGGG 
     3361 GCAGGACAGC AAGGGGGAGG ATTGGGAAGA CAATAGCAGG CATGCTGGGG ATGCGGTGGG 
     3421 CTCTATGGCT TCTGAGGCGG AAAGAACCAG CTGGGGCTCT AGGGGGTATC CCCACGCGCC 
     3481 CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT 
     3541 TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC 
     3601 CGGCTTTCCC CGTCAAGCTC TAAATCGGGG CATCCCTTTA GGGTTCCGAT TTAGTGCTTT 
     3661 ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC 
     3721 CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT 
     3781 GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT 
     3841 TTTGGGGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA 
     3901 TTAATTCTGT GGAATGTGTG TCAGTTAGGG TGTGGAAAGT CCCCAGGCTC CCCAGGCAGG 
     3961 CAGAAGTATG CAAAGCATGC ATCTCAATTA GTCAGCAACC AGGTGTGGAA AGTCCCCAGG 
     4021 CTCCCCAGCA GGCAGAAGTA TGCAAAGCAT GCATCTCAAT TAGTCAGCAA CCATAGTCCC 
     4081 GCCCCTAACT CCGCCCATCC CGCCCCTAAC TCCGCCCAGT TCCGCCCATT CTCCGCCCCA 
     4141 TGGCTGACTA ATTTTTTTTA TTTATGCAGA GGCCGAGGCC GCCTCTGCCT CTGAGCTATT 
     4201 CCAGAAGTAG TGAGGAGGCT TTTTTGGAGG CCTAGGCTTT TGCAAAAAGC TCCCGGGAGC 
     4261 TTGTATATCC ATTTTCGGAT CTGATCAAGA GACAGGATGA GGATCGTTTC GCATGATTGA 
     4321 ACAAGATGGA TTGCACGCAG GTTCTCCGGC CGCTTGGGTG GAGAGGCTAT TCGGCTATGA 
     4381 CTGGGCACAA CAGACAATCG GCTGCTCTGA TGCCGCCGTG TTCCGGCTGT CAGCGCAGGG 
     4441 GCGCCCGGTT CTTTTTGTCA AGACCGACCT GTCCGGTGCC CTGAATGAAC TGCAGGACGA 
     4501 GGCAGCGCGG CTATCGTGGC TGGCCACGAC GGGCGTTCCT TGCGCAGCTG TGCTCGACGT 
     4561 TGTCACTGAA GCGGGAAGGG ACTGGCTGCT ATTGGGCGAA GTGCCGGGGC AGGATCTCCT 
     4621 GTCATCTCAC CTTGCTCCTG CCGAGAAAGT ATCCATCATG GCTGATGCAA TGCGGCGGCT 
     4681 GCATACGCTT GATCCGGCTA CCTGCCCATT CGACCACCAA GCGAAACATC GCATCGAGCG 
     4741 AGCACGTACT CGGATGGAAG CCGGTCTTGT CGATCAGGAT GATCTGGACG AAGAGCATCA 
     4801 GGGGCTCGCG CCAGCCGAAC TGTTCGCCAG GCTCAAGGCG CGCATGCCCG ACGGCGAGGA 
     4861 TCTCGTCGTG ACCCATGGCG ATGCCTGCTT GCCGAATATC ATGGTGGAAA ATGGCCGCTT 
     4921 TTCTGGATTC ATCGACTGTG GCCGGCTGGG TGTGGCGGAC CGCTATCAGG ACATAGCGTT 
     4981 GGCTACCCGT GATATTGCTG AAGAGCTTGG CGGCGAATGG GCTGACCGCT TCCTCGTGCT 
     5041 TTACGGTATC GCCGCTCCCG ATTCGCAGCG CATCGCCTTC TATCGCCTTC TTGACGAGTT 
     5101 CTTCTGAGCG GGACTCTGGG GTTCGAAATG ACCGACCAAG CGACGCCCAA CCTGCCATCA 
     5161 CGAGATTTCG ATTCCACCGC CGCCTTCTAT GAAAGGTTGG GCTTCGGAAT CGTTTTCCGG 
     5221 GACGCCGGCT GGATGATCCT CCAGCGCGGG GATCTCATGC TGGAGTTCTT CGCCCACCCC 
     5281 AACTTGTTTA TTGCAGCTTA TAATGGTTAC AAATAAAGCA ATAGCATCAC AAATTTCACA 
     5341 AATAAAGCAT TTTTTTCACT GCATTCTAGT TGTGGTTTGT CCAAACTCAT CAATGTATCT 
     5401 TATCATGTCT GTATACCGTC GACCTCTAGC TAGAGCTTGG CGTAATCATG GTCATAGCTG 
     5461 TTTCCTGTGT GAAATTGTTA TCCGCTCACA ATTCCACACA ACATACGAGC CGGAAGCATA 
     5521 AAGTGTAAAG CCTGGGGTGC CTAATGAGTG AGCTAACTCA CATTAATTGC GTTGCGCTCA 
     5581 CTGCCCGCTT TCCAGTCGGG AAACCTGTCG TGCCAGCTGC ATTAATGAAT CGGCCAACGC 
     5641 GCGGGGAGAG GCGGTTTGCG TATTGGGCGC TCTTCCGCTT CCTCGCTCAC TGACTCGCTG 
     5701 CGCTCGGTCG TTCGGCTGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA 
     5761 TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC 
     5821 AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG 
     5881 CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC 
     5941 CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC 
     6001 GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCAATG CTCACGCTGT 
     6061 AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC 
     6121 GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA 
     6181 CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA 
     6241 GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG AAGGACAGTA 
     6301 TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA 
     6361 TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG 
     6421 CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG 
     6481 TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC 
     6541 TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT 
     6601 TGGTCTGACA GTTACCAATG CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT 
     6661 CGTTCATCCA TAGTTGCCTG ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA 
     6721 CCATCTGGCC CCAGTGCTGC AATGATACCG CGAGACCCAC GCTCACCGGC TCCAGATTTA 
     6781 TCAGCAATAA ACCAGCCAGC CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC 
     6841 GCCTCCATCC AGTCTATTAA TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT 
     6901 AGTTTGCGCA ACGTTGTTGC CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT 
     6961 ATGGCTTCAT TCAGCTCCGG TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG 
     7021 TGCAAAAAAG CGGTTAGCTC CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA 
     7081 GTGTTATCAC TCATGGTTAT GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA 
     7141 AGATGCTTTT CTGTGACTGG TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG 
     7201 CGACCGAGTT GCTCTTGCCC GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT 
     7261 TTAAAAGTGC TCATCATTGG AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG 
     7321 CTGTTGAGAT CCAGTTCGAT GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT 
     7381 ACTTTCACCA GCGTTTCTGG GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA 
     7441 ATAAGGGCGA CACGGAAATG TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC 
     7501 ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA 
     7561 CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGTC
//
