LOCUS       Exported               14538 bp ds-DNA    circular SYN 21-SEP-2016
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    pTK15_Lenti_dCas9_p300_P2A_Puro_No_gRNA
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14538)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 21, 2016 from SnapGene 2.3.2
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..14538
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             complement(344..355)
                     /note="FactorXa site"
     LTR             533..766
                     /note="3' LTR (Delta-U3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    798..1022
                     /note="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      1068..1496
                     /direction=RIGHT
                     /note="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        1510..1839
                     /note="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     promoter        1887..1934
                     /note="EM7 promoter"
                     /note="synthetic bacterial promoter "
     CDS             1953..2327
                     /note="ZeoR"
     polyA_signal    2457..2578
                     /note="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2623..2643)
                     /note="M13-rev"
     primer_bind     complement(2627..2643)
                     /note="M13 rev"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_binding    complement(2649..2671)
                     /note="LacO"
     protein_bind    2651..2667
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2675..2705)
                     /note="lac promoter"
                     /note="promoter for the E. coli lac operon"
     promoter        complement(2676..2705)
                     /note="lac"
     protein_bind    2720..2741
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding site"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(3029..3617)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3788..4648)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(4649..4753)
                     /gene="bla"
                     /note="AmpR promoter"
     enhancer        5019..5398
                     /note="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5400..5598
                     /note="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             5616..5796
                     /note="HIV-1 5 LTR"
     misc_feature    5843..5968
                     /note="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6461..6694
                     /note="RRE"
     misc_feature    7221..7338
                     /note="cPPT/CTS"
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    7415..7670
                     /note="EFS Promoter"
     CDS             7694..11797
                     /codon_start=1
                     /locus_tag="
                     "
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /note="dCas9"
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10 mutation in the 
                     RuvC-like domain and the H840A mutation in the HNH domain"
                     /protein_id="
                     "
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             11798..11845
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /note="nucleoplasmin NLS"
                     /translation="KRPAATKKAGQAKKKK"
     misc_feature    11852..13702
                     /note="p300 Core"
     CDS             13709..13732
                     /note="Flag tag"
     CDS             13742..13798
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /note="P2A"
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             13799..14395
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /note="PuroR"
                     /note="confers resistance to puromycin"
                     /translation="TEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIERV
                     TELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLAA
                     QQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETSA
                     PRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     misc_feature    14411..461
                     /note="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
ORIGIN
        1 cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt
       61 tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg
      121 cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac
      181 ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac
      241 tgacaattcc gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt
      301 tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc
      361 ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg
      421 ccctcagacg agtcggatct ccctttgggc cgcctccccg cgtcgacttt aagaccaatg
      481 acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg
      541 ctaattcact cccaacgaag acaagatctg ctttttgctt gtactgggtc tctctggtta
      601 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
      661 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
      721 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagggc ccgtttaaac
      781 ccgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc
      841 cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga
      901 aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga
      961 cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat
     1021 ggcttctgag gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag
     1081 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
     1141 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
     1201 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
     1261 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata
     1321 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
     1381 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc
     1441 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt
     1501 ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt
     1561 atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca
     1621 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta
     1681 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
     1741 ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag
     1801 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata
     1861 tccattttcg gatctgatca gcacgtgttg acaattaatc atcggcatag tatatcggca
     1921 tagtataata cgacaaggtg aggaactaaa ccatggccaa gttgaccagt gccgttccgg
     1981 tgctcaccgc gcgcgacgtc gccggagcgg tcgagttctg gaccgaccgg ctcgggttct
     2041 cccgggactt cgtggaggac gacttcgccg gtgtggtccg ggacgacgtg accctgttca
     2101 tcagcgcggt ccaggaccag gtggtgccgg acaacaccct ggcctgggtg tgggtgcgcg
     2161 gcctggacga gctgtacgcc gagtggtcgg aggtcgtgtc cacgaacttc cgggacgcct
     2221 ccgggccggc catgaccgag atcggcgagc agccgtgggg gcgggagttc gccctgcgcg
     2281 acccggccgg caactgcgtg cacttcgtgg ccgaggagca ggactgacac gtgctacgag
     2341 atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg
     2401 ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccaact
     2461 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     2521 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
     2581 atgtctgtat accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc
     2641 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
     2701 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
     2761 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
     2821 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     2881 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     2941 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     3001 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     3061 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     3121 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     3181 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     3241 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     3301 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     3361 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     3421 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
     3481 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     3541 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     3601 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     3661 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     3721 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     3781 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     3841 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     3901 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     3961 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     4021 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     4081 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     4141 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     4201 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     4261 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     4321 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     4381 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     4441 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     4501 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     4561 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     4621 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     4681 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     4741 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtcgacgga tcgggagatc
     4801 tcccgatccc ctatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca
     4861 gtatctgctc cctgcttgtg tgttggaggt cgctgagtag tgcgcgagca aaatttaagc
     4921 tacaacaagg caaggcttga ccgacaattg catgaagaat ctgcttaggg ttaggcgttt
     4981 tgcgctgctt cgcgatgtac gggccagata tacgcgttga cattgattat tgactagtta
     5041 ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac
     5101 ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc
     5161 aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt
     5221 ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac
     5281 gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac
     5341 cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt
     5401 gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac ggggatttcc
     5461 aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt
     5521 tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg
     5581 ggaggtctat ataagcagcg cgttttgcct gtactgggtc tctctggtta gaccagatct
     5641 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
     5701 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
     5761 tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa
     5821 agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg aagcgcgcac
     5881 ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta gcggaggcta
     5941 gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta gatcgcgatg
     6001 ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac atatagtatg
     6061 ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa catcagaagg
     6121 ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag aagaacttag
     6181 atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag agataaaaga
     6241 caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga ccaccgcaca
     6301 gcaagcggcc gctgatcttc agacctggag gaggagatat gagggacaat tggagaagtg
     6361 aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc accaaggcaa
     6421 agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg ttccttgggt
     6481 tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg gtacaggcca
     6541 gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct attgaggcgc
     6601 aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca agaatcctgg
     6661 ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc tctggaaaac
     6721 tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct ctggaacaga
     6781 tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac acaagcttaa
     6841 tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg
     6901 aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg ctgtggtata
     6961 taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt tttgctgtac
     7021 tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag acccacctcc
     7081 caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga gagagagaca
     7141 gagacagatc cattcgatta gtgaacggat cggcactgcg tgcgccaatt ctgcagacaa
     7201 atggcagtat tcatccacaa ttttaaaaga aaagggggga ttggggggta cagtgcaggg
     7261 gaaagaatag tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt
     7321 acaaaaattc aaaattttcg ggtttattac agggacagca gagatccagt ttggttaatt
     7381 aaggtaccga ggcgataggg ttaagggagc tagctaggtc ttgaaaggag tgggaattgg
     7441 ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca gtccccgaga agttgggggg
     7501 aggggtcggc aattgatccg gtgcctagag aaggtggcgc ggggtaaact gggaaagtga
     7561 tgtcgtgtac tggctccgcc tttttcccga gggtggggga gaaccgtata taagtgcagt
     7621 agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc agaacacagg accggttcta
     7681 gagcgctgcc accatggaca agaagtacag catcggcctg gccatcggca ccaactctgt
     7741 gggctgggcc gtgatcaccg acgagtacaa ggtgcccagc aagaaattca aggtgctggg
     7801 caacaccgac cggcacagca tcaagaagaa cctgatcgga gccctgctgt tcgacagcgg
     7861 cgaaacagcc gaggccaccc ggctgaagag aaccgccaga agaagataca ccagacggaa
     7921 gaaccggatc tgctatctgc aagagatctt cagcaacgag atggccaagg tggacgacag
     7981 cttcttccac agactggaag agtccttcct ggtggaagag gataagaagc acgagcggca
     8041 ccccatcttc ggcaacatcg tggacgaggt ggcctaccac gagaagtacc ccaccatcta
     8101 ccacctgaga aagaaactgg tggacagcac cgacaaggcc gacctgcggc tgatctatct
     8161 ggccctggcc cacatgatca agttccgggg ccacttcctg atcgagggcg acctgaaccc
     8221 cgacaacagc gacgtggaca agctgttcat ccagctggtg cagacctaca accagctgtt
     8281 cgaggaaaac cccatcaacg ccagcggcgt ggacgccaag gccatcctgt ctgccagact
     8341 gagcaagagc agacggctgg aaaatctgat cgcccagctg cccggcgaga agaagaatgg
     8401 cctgttcgga aacctgattg ccctgagcct gggcctgacc cccaacttca agagcaactt
     8461 cgacctggcc gaggatgcca aactgcagct gagcaaggac acctacgacg acgacctgga
     8521 caacctgctg gcccagatcg gcgaccagta cgccgacctg tttctggccg ccaagaacct
     8581 gtccgacgcc atcctgctga gcgacatcct gagagtgaac accgagatca ccaaggcccc
     8641 cctgagcgcc tctatgatca agagatacga cgagcaccac caggacctga ccctgctgaa
     8701 agctctcgtg cggcagcagc tgcctgagaa gtacaaagag attttcttcg accagagcaa
     8761 gaacggctac gccggctaca ttgacggcgg agccagccag gaagagttct acaagttcat
     8821 caagcccatc ctggaaaaga tggacggcac cgaggaactg ctcgtgaagc tgaacagaga
     8881 ggacctgctg cggaagcagc ggaccttcga caacggcagc atcccccacc agatccacct
     8941 gggagagctg cacgccattc tgcggcggca ggaagatttt tacccattcc tgaaggacaa
     9001 ccgggaaaag atcgagaaga tcctgacctt ccgcatcccc tactacgtgg gccctctggc
     9061 caggggaaac agcagattcg cctggatgac cagaaagagc gaggaaacca tcaccccctg
     9121 gaacttcgag gaagtggtgg acaagggcgc ttccgcccag agcttcatcg agcggatgac
     9181 caacttcgat aagaacctgc ccaacgagaa ggtgctgccc aagcacagcc tgctgtacga
     9241 gtacttcacc gtgtataacg agctgaccaa agtgaaatac gtgaccgagg gaatgagaaa
     9301 gcccgccttc ctgagcggcg agcagaaaaa ggccatcgtg gacctgctgt tcaagaccaa
     9361 ccggaaagtg accgtgaagc agctgaaaga ggactacttc aagaaaatcg agtgcttcga
     9421 ctccgtggaa atctccggcg tggaagatcg gttcaacgcc tccctgggca cataccacga
     9481 tctgctgaaa attatcaagg acaaggactt cctggacaat gaggaaaacg aggacattct
     9541 ggaagatatc gtgctgaccc tgacactgtt tgaggacaga gagatgatcg aggaacggct
     9601 gaaaacctat gcccacctgt tcgacgacaa agtgatgaag cagctgaagc ggcggagata
     9661 caccggctgg ggcaggctga gccggaagct gatcaacggc atccgggaca agcagtccgg
     9721 caagacaatc ctggatttcc tgaagtccga cggcttcgcc aacagaaact tcatgcagct
     9781 gatccacgac gacagcctga cctttaaaga ggacatccag aaagcccagg tgtccggcca
     9841 gggcgatagc ctgcacgagc acattgccaa tctggccggc agccccgcca ttaagaaggg
     9901 catcctgcag acagtgaagg tggtggacga gctcgtgaaa gtgatgggcc ggcacaagcc
     9961 cgagaacatc gtgatcgaaa tggccagaga gaaccagacc acccagaagg gacagaagaa
    10021 cagccgcgag agaatgaagc ggatcgaaga gggcatcaaa gagctgggca gccagatcct
    10081 gaaagaacac cccgtggaaa acacccagct gcagaacgag aagctgtacc tgtactacct
    10141 gcagaatggg cgggatatgt acgtggacca ggaactggac atcaaccggc tgtccgacta
    10201 cgatgtggac gccatcgtgc ctcagagctt tctgaaggac gactccatcg acaacaaggt
    10261 gctgaccaga agcgacaaga accggggcaa gagcgacaac gtgccctccg aagaggtcgt
    10321 gaagaagatg aagaactact ggcggcagct gctgaacgcc aagctgatta cccagagaaa
    10381 gttcgacaat ctgaccaagg ccgagagagg cggcctgagc gaactggata aggccggctt
    10441 catcaagaga cagctggtgg aaacccggca gatcacaaag cacgtggcac agatcctgga
    10501 ctcccggatg aacactaagt acgacgagaa tgacaagctg atccgggaag tgaaagtgat
    10561 caccctgaag tccaagctgg tgtccgattt ccggaaggat ttccagtttt acaaagtgcg
    10621 cgagatcaac aactaccacc acgcccacga cgcctacctg aacgccgtcg tgggaaccgc
    10681 cctgatcaaa aagtacccta agctggaaag cgagttcgtg tacggcgact acaaggtgta
    10741 cgacgtgcgg aagatgatcg ccaagagcga gcaggaaatc ggcaaggcta ccgccaagta
    10801 cttcttctac agcaacatca tgaacttttt caagaccgag attaccctgg ccaacggcga
    10861 gatccggaag cggcctctga tcgagacaaa cggcgaaacc ggggagatcg tgtgggataa
    10921 gggccgggat tttgccaccg tgcggaaagt gctgagcatg ccccaagtga atatcgtgaa
    10981 aaagaccgag gtgcagacag gcggcttcag caaagagtct atcctgccca agaggaacag
    11041 cgataagctg atcgccagaa agaaggactg ggaccctaag aagtacggcg gcttcgacag
    11101 ccccaccgtg gcctattctg tgctggtggt ggccaaagtg gaaaagggca agtccaagaa
    11161 actgaagagt gtgaaagagc tgctggggat caccatcatg gaaagaagca gcttcgagaa
    11221 gaatcccatc gactttctgg aagccaaggg ctacaaagaa gtgaaaaagg acctgatcat
    11281 caagctgcct aagtactccc tgttcgagct ggaaaacggc cggaagagaa tgctggcctc
    11341 tgccggcgaa ctgcagaagg gaaacgaact ggccctgccc tccaaatatg tgaacttcct
    11401 gtacctggcc agccactatg agaagctgaa gggctccccc gaggataatg agcagaaaca
    11461 gctgtttgtg gaacagcaca agcactacct ggacgagatc atcgagcaga tcagcgagtt
    11521 ctccaagaga gtgatcctgg ccgacgctaa tctggacaaa gtgctgtccg cctacaacaa
    11581 gcaccgggat aagcccatca gagagcaggc cgagaatatc atccacctgt ttaccctgac
    11641 caatctggga gcccctgccg ccttcaagta ctttgacacc accatcgacc ggaagaggta
    11701 caccagcacc aaagaggtgc tggacgccac cctgatccac cagagcatca ccggcctgta
    11761 cgagacacgg atcgacctgt ctcagctggg aggcgacaag cgacctgccg ccacaaagaa
    11821 ggctggacag gctaagaaga agaaaggatc cattttcaaa ccagaagaac tacgacaggc
    11881 actgatgcca actttggagg cactttaccg tcaggatcca gaatcccttc cctttcgtca
    11941 acctgtggac cctcagcttt taggaatccc tgattacttt gatattgtga agagccccat
    12001 ggatctttct accattaaga ggaagttaga cactggacag tatcaggagc cctggcagta
    12061 tgtcgatgat atttggctta tgttcaataa tgcctggtta tataaccgga aaacatcacg
    12121 ggtatacaaa tactgctcca agctctctga ggtctttgaa caagaaattg acccagtgat
    12181 gcaaagcctt ggatactgtt gtggcagaaa gttggagttc tctccacaga cactgtgttg
    12241 ctacggcaaa cagttgtgca caatacctcg tgatgccact tattacagtt accagaacag
    12301 gtatcatttc tgtgagaagt gtttcaatga gatccaaggg gagagcgttt ctttggggga
    12361 tgacccttcc cagcctcaaa ctacaataaa taaagaacaa ttttccaaga gaaaaaatga
    12421 cacactggat cctgaactgt ttgttgaatg tacagagtgc ggaagaaaga tgcatcagat
    12481 ctgtgtcctt caccatgaga tcatctggcc tgctggattc gtctgtgatg gctgtttaaa
    12541 gaaaagtgca cgaactagga aagaaaataa gttttctgct aaaaggttgc catctaccag
    12601 acttggcacc tttctagaga atcgtgtgaa tgactttctg aggcgacaga atcaccctga
    12661 gtcaggagag gtcactgtta gagtagttca tgcttctgac aaaaccgtgg aagtaaaacc
    12721 aggcatgaaa gcaaggtttg tggacagtgg agagatggca gaatcctttc cataccgaac
    12781 caaagccctc tttgcctttg aagaaattga tggtgttgac ctgtgcttct ttggcatgca
    12841 tgttcaagag tatggctctg actgccctcc acccaaccag aggagagtat acatatctta
    12901 cctcgatagt gttcatttct tccgtcctaa atgcttgagg actgcagtct atcatgaaat
    12961 cctaattgga tatttagaat atgtcaagaa attaggttac acaacagggc atatttgggc
    13021 atgtccacca agtgagggag atgattatat cttccattgc catcctcctg accagaagat
    13081 acccaagccc aagcgactgc aggaatggta caaaaaaatg cttgacaagg ctgtatcaga
    13141 gcgtattgtc catgactaca aggatatttt taaacaagct actgaagata gattaacaag
    13201 tgcaaaggaa ttgccttatt tcgagggtga tttctggccc aatgttctgg aagaaagcat
    13261 taaggaactg gaacaggagg aagaagagag aaaacgagag gaaaacacca gcaatgaaag
    13321 cacagatgtg accaagggag acagcaaaaa tgctaaaaag aagaataata agaaaaccag
    13381 caaaaataag agcagcctga gtaggggcaa caagaagaaa cccgggatgc ccaatgtatc
    13441 taacgacctc tcacagaaac tatatgccac catggagaag cataaagagg tcttctttgt
    13501 gatccgcctc attgctggcc ctgctgccaa ctccctgcct cccattgttg atcctgatcc
    13561 tctcatcccc tgcgatctga tggatggtcg ggatgcgttt ctcacgctgg caagggacaa
    13621 gcacctggag ttctcttcac tccgaagagc ccagtggtcc accatgtgca tgctggtgga
    13681 gctgcacacg cagagccagg acggatccga ttacaaagac gatgacgata agggatccgg
    13741 cgcaacaaac ttctctctgc tgaaacaagc cggagatgtc gaagagaatc ctggaccgac
    13801 cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac
    13861 cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgatc cggaccgcca
    13921 catcgagcgg gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg
    13981 caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc cggagagcgt
    14041 cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg
    14101 gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc
    14161 gtggttcctg gccaccgtcg gagtctcgcc cgaccaccag ggcaagggtc tgggcagcgc
    14221 cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac
    14281 ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt
    14341 cgaggtgccc gaaggaccgc gcacctggtg catgacccgc aagcccggtg cctgaacgcg
    14401 ttaagtcgac aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa
    14461 ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat
    14521 tgcttcccgt atggcttt
//
