LOCUS       Exported               15860 bp ds-DNA    circular SYN 22-SEP-2016
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    TKp9-FUGW-dCas9-KRAB-PGK-Hygro
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15860)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Sep 22, 2016 from SnapGene 2.3.2
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..15860
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        238..617
                     /note="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /note="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             835..1015
                     /note="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /note="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1680..1913
                     /note="RRE"
                     /note="retroviral response element"
     misc_feature    2440..2557
                     /note="cPPT/CTS"
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    2602..2635
                     /bound_moiety="Cre recombinase"
                     /note="loxP"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     CDS             complement(2673..2741)
                     /note="LacZ alpha"
     primer_bind     2813..2829
                     /note="M13 fwd"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2839..2857
                     /note="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(2905..2923)
                     /note="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2944..2960)
                     /note="M13 rev"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2968..2984
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2992..3022)
                     /note="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    3037..3058
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding site"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        3131..4333
                     /note="hUbC promoter"
     CDS             4371..4436
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /note="3xFLAG"
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             4443..4463
                     /codon_start=1
                     /locus_tag="
                     "
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /note="SV40 NLS"
                     /note="
                     "
                     /protein_id="
                     "
                     /translation="PKKKRKV"
     CDS             4473..8576
                     /codon_start=1
                     /locus_tag="
                     "
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /note="dCas9"
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10 mutation in the 
                     RuvC-like domain and the H840A mutation in the HNH domain"
                     /protein_id="
                     "
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             8589..8609
                     /codon_start=1
                     /locus_tag="
                     "
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /note="SV40 NLS"
                     /note="
                     "
                     /protein_id="
                     "
                     /translation="PKKKRKV"
     CDS             8615..8903
                     /note="KRAB"
     CDS             8904..8924
                     /codon_start=1
                     /locus_tag="
                     "
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /note="SV40 NLS"
                     /note="
                     "
                     /protein_id="
                     "
                     /translation="PKKKRKV"
     promoter        8936..9435
                     /note="PGK promoter"
                     /note="mouse phosphoglycerate kinase 1 promoter"
     CDS             9453..10478
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /note="HygR"
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     protein_bind    10494..10527
                     /bound_moiety="Cre recombinase"
                     /note="loxP"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     misc_feature    10552..11140
                     /note="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(11023..11034)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /note="Factor Xa site"
                     /translation="IEGR"
     primer_bind     complement(11143..11159)
                     /note="KS primer"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    11328..11343
                     /note="cPPT"
     LTR             11665..11845
                     /note="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     polyA_signal    11877..12101
                     /note="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      12147..12575
                     /direction=RIGHT
                     /note="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        12589..12918
                     /note="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     rep_origin      12769..12904
                     /note="SV40 ori"
                     /note="SV40 origin of replication"
     promoter        12966..13013
                     /note="EM7 promoter"
                     /note="synthetic bacterial promoter "
     CDS             13032..13406
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /note="BleoR"
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    13536..13657
                     /note="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(13706..13722)
                     /note="M13 rev"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    13730..13746
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(13754..13784)
                     /note="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    13799..13820
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding site"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(14108..14696)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(14867..15727)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(15728..15832)
                     /gene="bla"
                     /note="AmpR promoter"
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaatta aataacttcg tatagcatac attatacgaa gttatcgtct
     2641 cagatagtgg tggcgccgct acagggcgcg tcccattcgc cattcaggct gcgcaactgt
     2701 tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
     2761 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
     2821 acggccagtg agcgcgcgta atacgactca ctatagggcg aattgggtac cgggcccccc
     2881 ctcgaggtcc tccagctttt gttcccttta gtgagggtta attgcgcgct tggcgtaatc
     2941 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
     3001 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
     3061 tgcgttgcgc tcactgcccg ctttccactg catgacaatg agacgttaat taagggtgca
     3121 gcggcctccg cgccgggttt tggcgcctcc cgcgggcgcc cccctcctca cggcgagcgc
     3181 tgccacgtca gacgaagggc gcaggagcgt tcctgatcct tccgcccgga cgctcaggac
     3241 agcggcccgc tgctcataag actcggcctt agaaccccag tatcagcaga aggacatttt
     3301 aggacgggac ttgggtgact ctagggcact ggttttcttt ccagagagcg gaacaggcga
     3361 ggaaaagtag tcccttctcg gcgattctgc ggagggatct ccgtggggcg gtgaacgccg
     3421 atgattatat aaggacgcgc cgggtgtggc acagctagtt ccgtcgcagc cgggatttgg
     3481 gtcgcggttc ttgtttgtgg atcgctgtga tcgtcacttg gtgagttgcg ggctgctggg
     3541 ctggccgggg ctttcgtggc cgccgggccg ctcggtggga cggaagcgtg tggagagacc
     3601 gccaagggct gtagtctggg tccgcgagca aggttgccct gaactggggg ttggggggag
     3661 cgcacaaaat ggcggctgtt cccgagtctt gaatggaaga cgcttgtaag gcgggctgtg
     3721 aggtcgttga aacaaggtgg ggggcatggt gggcggcaag aacccaaggt cttgaggcct
     3781 tcgctaatgc gggaaagctc ttattcgggt gagatgggct ggggcaccat ctggggaccc
     3841 tgacgtgaag tttgtcactg actggagaac tcgggtttgt cgtctggttg cgggggcggc
     3901 agttatgcgg tgccgttggg cagtgcaccc gtacctttgg gagcgcgcgc ctcgtcgtgt
     3961 cgtgacgtca cccgttctgt tggcttataa tgcagggtgg ggccacctgc cggtaggtgt
     4021 gcggtaggct tttctccgtc gcaggacgca gggttcgggc ctagggtagg ctctcctgaa
     4081 tcgacaggcg ccggacctct ggtgagggga gggataagtg aggcgtcagt ttctttggtc
     4141 ggttttatgt acctatcttc ttaagtagct gaagctccgg ttttgaacta tgcgctcggg
     4201 gttggcgagt gtgttttgtg aagtttttta ggcacctttt gaaatgtaat catttgggtc
     4261 aatatgtaat tttcagtgtt agactagtaa attgtccgct aaattctggc cgtttttggc
     4321 ttttttgtta gacgaagctt gggctgcagg tcgactctag agccaccatg gactacaaag
     4381 accatgacgg tgattataaa gatcatgaca tcgattacaa ggatgacgat gacaagatgg
     4441 cccccaagaa gaagaggaag gtgggccgcg gaatggacaa gaagtactcc attgggctcg
     4501 ccatcggcac aaacagcgtc ggctgggccg tcattacgga cgagtacaag gtgccgagca
     4561 aaaaattcaa agttctgggc aataccgatc gccacagcat aaagaagaac ctcattggcg
     4621 ccctcctgtt cgactccggg gaaaccgccg aagccacgcg gctcaaaaga acagcacggc
     4681 gcagatatac ccgcagaaag aatcggatct gctacctgca ggagatcttt agtaatgaga
     4741 tggctaaggt ggatgactct ttcttccata ggctggagga gtcctttttg gtggaggagg
     4801 ataaaaagca cgagcgccac ccaatctttg gcaatatcgt ggacgaggtg gcgtaccatg
     4861 aaaagtaccc aaccatatat catctgagga agaagcttgt agacagtact gataaggctg
     4921 acttgcggtt gatctatctc gcgctggcgc atatgatcaa atttcgggga cacttcctca
     4981 tcgaggggga cctgaaccca gacaacagcg atgtcgacaa actctttatc caactggttc
     5041 agacttacaa tcagcttttc gaagagaacc cgatcaacgc atccggagtt gacgccaaag
     5101 caatcctgag cgctaggctg tccaaatccc ggcggctcga aaacctcatc gcacagctcc
     5161 ctggggagaa gaagaacggc ctgtttggta atcttatcgc cctgtcactc gggctgaccc
     5221 ccaactttaa atctaacttc gacctggccg aagatgccaa gcttcaactg agcaaagaca
     5281 cctacgatga tgatctcgac aatctgctgg cccagatcgg cgaccagtac gcagaccttt
     5341 ttttggcggc aaagaacctg tcagacgcca ttctgctgag tgatattctg cgagtgaaca
     5401 cggagatcac caaagctccg ctgagcgcta gtatgatcaa gcgctatgat gagcaccacc
     5461 aagacttgac tttgctgaag gcccttgtca gacagcaact gcctgagaag tacaaggaaa
     5521 ttttcttcga tcagtctaaa aatggctacg ccggatacat tgacggcgga gcaagccagg
     5581 aggaatttta caaatttatt aagcccatct tggaaaaaat ggacggcacc gaggagctgc
     5641 tggtaaagct taacagagaa gatctgttgc gcaaacagcg cactttcgac aatggaagca
     5701 tcccccacca gattcacctg ggcgaactgc acgctatcct caggcggcaa gaggatttct
     5761 accccttttt gaaagataac agggaaaaga ttgagaaaat cctcacattt cggataccct
     5821 actatgtagg ccccctcgcc cggggaaatt ccagattcgc gtggatgact cgcaaatcag
     5881 aagagaccat cactccctgg aacttcgagg aagtcgtgga taagggggcc tctgcccagt
     5941 ccttcatcga aaggatgact aactttgata aaaatctgcc taacgaaaag gtgcttccta
     6001 aacactctct gctgtacgag tacttcacag tttataacga gctcaccaag gtcaaatacg
     6061 tcacagaagg gatgagaaag ccagcattcc tgtctggaga gcagaagaaa gctatcgtgg
     6121 acctcctctt caagacgaac cggaaagtta ccgtgaaaca gctcaaagaa gactatttca
     6181 aaaagattga atgtttcgac tctgttgaaa tcagcggagt ggaggatcgc ttcaacgcat
     6241 ccctgggaac gtatcacgat ctcctgaaaa tcattaaaga caaggacttc ctggacaatg
     6301 aggagaacga ggacattctt gaggacattg tcctcaccct tacgttgttt gaagataggg
     6361 agatgattga agaacgcttg aaaacttacg ctcatctctt cgacgacaaa gtcatgaaac
     6421 agctcaagag gcgccgatat acaggatggg ggcggctgtc aagaaaactg atcaatggga
     6481 tccgagacaa gcagagtgga aagacaatcc tggattttct taagtccgat ggatttgcca
     6541 accggaactt catgcagttg atccatgatg actctctcac ctttaaggag gacatccaga
     6601 aagcacaagt ttctggccag ggggacagtc ttcacgagca catcgctaat cttgcaggta
     6661 gcccagctat caaaaaggga atactgcaga ccgttaaggt cgtggatgaa ctcgtcaaag
     6721 taatgggaag gcataagccc gagaatatcg ttatcgagat ggcccgagag aaccaaacta
     6781 cccagaaggg acagaagaac agtagggaaa ggatgaagag gattgaagag ggtataaaag
     6841 aactggggtc ccaaatcctt aaggaacacc cagttgaaaa cacccagctt cagaatgaga
     6901 agctctacct gtactacctg cagaacggca gggacatgta cgtggatcag gaactggaca
     6961 tcaatcggct ctccgactac gacgtggatg ccatcgtgcc ccagtctttt ctcaaagatg
     7021 attctattga taataaagtg ttgacaagat ccgataaaaa tagagggaag agtgataacg
     7081 tcccctcaga agaagttgtc aagaaaatga aaaattattg gcggcagctg ctgaacgcca
     7141 aactgatcac acaacggaag ttcgataatc tgactaaggc tgaacgaggt ggcctgtctg
     7201 agttggataa agccggcttc atcaaaaggc agcttgttga gacacgccag atcaccaagc
     7261 acgtggccca aattctcgat tcacgcatga acaccaagta cgatgaaaat gacaaactga
     7321 ttcgagaggt gaaagttatt actctgaagt ctaagctggt ctcagatttc agaaaggact
     7381 ttcagtttta taaggtgaga gagatcaaca attaccacca tgcgcatgat gcctacctga
     7441 atgcagtggt aggcactgca cttatcaaaa aatatcccaa gcttgaatct gaatttgttt
     7501 acggagacta taaagtgtac gatgttagga aaatgatcgc aaagtctgag caggaaatag
     7561 gcaaggccac cgctaagtac ttcttttaca gcaatattat gaattttttc aagaccgaga
     7621 ttacactggc caatggagag attcggaagc gaccacttat cgaaacaaac ggagaaacag
     7681 gagaaatcgt gtgggacaag ggtagggatt tcgcgacagt ccggaaggtc ctgtccatgc
     7741 cgcaggtgaa catcgttaaa aagaccgaag tacagaccgg aggcttctcc aaggaaagta
     7801 tcctcccgaa aaggaacagc gacaagctga tcgcacgcaa aaaagattgg gaccccaaga
     7861 aatacggcgg attcgattct cctacagtcg cttacagtgt actggttgtg gccaaagtgg
     7921 agaaagggaa gtctaaaaaa ctcaaaagcg tcaaggaact gctgggcatc acaatcatgg
     7981 agcgatcaag cttcgaaaaa aaccccatcg actttctcga ggcgaaagga tataaagagg
     8041 tcaaaaaaga cctcatcatt aagcttccca agtactctct ctttgagctt gaaaacggcc
     8101 ggaaacgaat gctcgctagt gcgggcgagc tgcagaaagg taacgagctg gcactgccct
     8161 ctaaatacgt taatttcttg tatctggcca gccactatga aaagctcaaa gggtctcccg
     8221 aagataatga gcagaagcag ctgttcgtgg aacaacacaa acactacctt gatgagatca
     8281 tcgagcaaat aagcgaattc tccaaaagag tgatcctcgc cgacgctaac ctcgataagg
     8341 tgctttctgc ttacaataag cacagggata agcccatcag ggagcaggca gaaaacatta
     8401 tccacttgtt tactctgacc aacttgggcg cgcctgcagc cttcaagtac ttcgacacca
     8461 ccatagacag aaagcggtac acctctacaa aggaggtcct ggacgccaca ctgattcatc
     8521 agtcaattac ggggctctat gaaacaagaa tcgacctctc tcagctcggt ggagacagca
     8581 gggctgaccc caagaagaag aggaaggtgg ctagcgatgc taagtcactg actgcctggt
     8641 cccggacact ggtgaccttc aaggatgtgt ttgtggactt caccagggag gagtggaagc
     8701 tgctggacac tgctcagcag atcctgtaca gaaatgtgat gctggagaac tataagaacc
     8761 tggtttcctt gggttatcag cttactaagc cagatgtgat cctccggttg gagaagggag
     8821 aagagccctg gctggtggag agagaaattc accaagagac ccatcctgat tcagagactg
     8881 catttgaaat caaatcatca gttccgaaaa agaaacgcaa agtttgagct agcccgggta
     8941 ggggaggcgc ttttcccaag gcagtctgga gcatgcgctt tagcagcccc gctgggcact
     9001 tggcgctaca caagtggcct ctggcctcgc acacattcca catccaccgg taggcgccaa
     9061 ccggctccgt tctttggtgg ccccttcgcg ccaccttcta ctcctcccct agtcaggaag
     9121 ttcccccccg ccccgcagct cgcgtcgtgc aggacgtgac aaatggaagt agcacgtctc
     9181 actagtctcg tgcagatgga cagcaccgct gagcaatgga agcgggtagg cctttggggc
     9241 agcggccaat agcagctttg ctccttcgct ttctgggctc agaggctggg aaggggtggg
     9301 tccgggggcg ggctcagggg cgggctcagg ggcggggcgg gcgcccgaag gtcctccgga
     9361 ggcccggcat tctgcacgct tcaaaagcgc acgtctgccg cgctgttctc ctcttcctca
     9421 tctccgggcc tttcgacctg catcccgcca ccatgaaaaa gcctgaactc accgcgacgt
     9481 ctgtcgagaa gtttctgatc gaaaagttcg acagcgtctc cgacctgatg cagctctcgg
     9541 agggcgaaga atctcgtgct ttcagcttcg atgtaggagg gcgtggatat gtcctgcggg
     9601 taaatagctg cgccgatggt ttctacaaag atcgttatgt ttatcggcac tttgcatcgg
     9661 ccgcgctccc gattccggaa gtgcttgaca ttggggaatt cagcgagagc ctgacctatt
     9721 gcatctcccg ccgtgcacag ggtgtcacgt tgcaagacct gcctgaaacc gaactgcccg
     9781 ctgttctgca gccggtcgcg gaggccatgg atgcgatcgc tgcggccgat cttagccaga
     9841 cgagcgggtt cggcccattc ggaccgcaag gaatcggtca atacactaca tggcgtgatt
     9901 tcatatgcgc gattgctgat ccccatgtgt atcactggca aactgtgatg gacgacaccg
     9961 tcagtgcgtc cgtcgcgcag gctctcgatg agctgatgct ttgggccgag gactgccccg
    10021 aagtccggca cctcgtgcac gcggatttcg gctccaacaa tgtcctgacg gacaatggcc
    10081 gcataacagc ggtcattgac tggagcgagg cgatgttcgg ggattcccaa tacgaggtcg
    10141 ccaacatctt cttctggagg ccgtggttgg cttgtatgga gcagcagacg cgctacttcg
    10201 agcggaggca tccggagctt gcaggatcgc cgcggctccg ggcgtatatg ctccgcattg
    10261 gtcttgacca actctatcag agcttggttg acggcaattt cgatgatgca gcttgggcgc
    10321 agggtcgatg cgacgcaatc gtccgatccg gagccgggac tgtcgggcgt acacaaatcg
    10381 cccgcagaag cgcggccgtc tggaccgatg gctgtgtaga agtactcgcc gatagtggaa
    10441 accgacgccc cagcactcgt ccgagggcaa aggaatagac cggttgataa tagataactt
    10501 cgtatagcat acattatacg aagttatgaa ttcgatatca agcttatcga taatcaacct
    10561 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg
    10621 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc
    10681 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt
    10741 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc
    10801 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg
    10861 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact
    10921 gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt
    10981 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg
    11041 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc
    11101 cctcagacga gtcggatctc cctttgggcc gcctccccgc atcgataccg tcgacctcga
    11161 gacctagaaa aacatggagc aatcacaagt agcaatacag cagctaccaa tgctgattgt
    11221 gcctggctag aagcacaaga ggaggaggag gtgggttttc cagtcacacc tcaggtacct
    11281 ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg
    11341 ggactggaag ggctaattca ctcccaacga agacaagata tccttgatct gtggatctac
    11401 cacacacaag gctacttccc tgattggcag aactacacac cagggccagg gatcagatat
    11461 ccactgacct ttggatggtg ctacaagcta gtaccagttg agcaagagaa ggtagaagaa
    11521 gccaatgaag gagagaacac ccgcttgtta caccctgtga gcctgcatgg gatggatgac
    11581 ccggagagag aagtattaga gtggaggttt gacagccgcc tagcatttca tcacatggcc
    11641 cgagagctgc atccggactg tactgggtct ctctggttag accagatctg agcctgggag
    11701 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
    11761 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
    11821 tagtcagtgt ggaaaatctc tagcagggcc cgtttaaacc cgctgatcag cctcgactgt
    11881 gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga
    11941 aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag
    12001 taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga
    12061 agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg cggaaagaac
    12121 cagctggggc tctagggggt atccccacgc gccctgtagc ggcgcattaa gcgcggcggg
    12181 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
    12241 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
    12301 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
    12361 ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
    12421 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
    12481 tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa
    12541 aaatgagctg atttaacaaa aatttaacgc gaattaattc tgtggaatgt gtgtcagtta
    12601 gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
    12661 tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
    12721 atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta
    12781 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
    12841 gaggccgagg ccgcctctgc ctctgagcta ttccagaagt agtgaggagg cttttttgga
    12901 ggcctaggct tttgcaaaaa gctcccggga gcttgtatat ccattttcgg atctgatcag
    12961 cacgtgttga caattaatca tcggcatagt atatcggcat agtataatac gacaaggtga
    13021 ggaactaaac catggccaag ttgaccagtg ccgttccggt gctcaccgcg cgcgacgtcg
    13081 ccggagcggt cgagttctgg accgaccggc tcgggttctc ccgggacttc gtggaggacg
    13141 acttcgccgg tgtggtccgg gacgacgtga ccctgttcat cagcgcggtc caggaccagg
    13201 tggtgccgga caacaccctg gcctgggtgt gggtgcgcgg cctggacgag ctgtacgccg
    13261 agtggtcgga ggtcgtgtcc acgaacttcc gggacgcctc cgggccggcc atgaccgaga
    13321 tcggcgagca gccgtggggg cgggagttcg ccctgcgcga cccggccggc aactgcgtgc
    13381 acttcgtggc cgaggagcag gactgacacg tgctacgaga tttcgattcc accgccgcct
    13441 tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc
    13501 gcggggatct catgctggag ttcttcgccc accccaactt gtttattgca gcttataatg
    13561 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
    13621 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctgtata ccgtcgacct
    13681 ctagctagag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
    13741 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
    13801 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
    13861 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
    13921 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
    13981 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
    14041 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
    14101 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
    14161 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
    14221 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
    14281 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
    14341 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
    14401 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
    14461 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
    14521 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
    14581 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
    14641 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
    14701 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
    14761 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
    14821 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
    14881 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
    14941 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
    15001 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
    15061 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
    15121 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
    15181 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
    15241 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
    15301 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
    15361 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
    15421 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
    15481 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
    15541 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
    15601 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
    15661 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
    15721 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
    15781 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
    15841 cccgaaaagt gccacctgac
//
