LOCUS       pRSF-Ivy-his-hLY        4514 bp    DNA     circular     14-FEB-2013
DEFINITION  pRSFDuet-1/Kan.
SOURCE      
  ORGANISM  
COMMENT     http://www.informaxinc.com/
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|653768342|
COMMENT     VNTDBDATE|653769634|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pRSF-Ivy-his-hLYZ|
COMMENT     VNTAUTHORNAME|Karl E. Griswold|
COMMENT     VNTOAUTHORNAME|EMD Biosciences Inc.|
COMMENT     VNTOAUTHORTEL|608-442-4000|
COMMENT     VNTOAUTHOREML|novagen@emdbiosciences.com|
COMMENT     VNTREPLTYPE|Plasmid
COMMENT     VNTEXTCHREPL|Bacteria
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pRSF-Ivy-his-hLYZ" 0 4514 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 0 0 "MAIN" 1 1 1 1 0 0 1 1 0 1 10 10 4294967295 50 0 
COMMENT        1 0 (CHomObj 0 0 0 3 75) (CWordArray) (CWordArray) 
COMMENT        (CStringList "AvaI" "BamHI" "EcoRI" "HindIII" "NcoI" "NdeI" "XbaI" 
COMMENT         "XhoI") (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") 
COMMENT        (CObList) 1 "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 0 "MAIN" 0 0 30 
COMMENT        0 (CProteinMotifSearchObject 70 20 1 1 1 1 0 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 0 4294967295 1 4514 0 0 0 0 0 0 0 0) (CStringList) 
COMMENT       (CStringList) (CObList) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList 
COMMENT        #0=(CRSite (CStringList) "AvaI" "cycgrg" 2 0 2 1040 0 1932 0 0 "") 
COMMENT        #1=(CRSite (CStringList) "BamHI" "ggatcc" 2 0 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "HindIII" "aagctt" 2 0 1 484 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "NcoI" "ccatgg" 2 0 1 70 0 0 "") 
COMMENT        #5=(CRSite (CStringList) "NdeI" "catatg" 3 0 1 639 0 0 "") 
COMMENT        #6=(CRSite (CStringList) "XbaI" "tctaga" 2 0 1 3100 0 0 "") 
COMMENT        #7=(CRSite (CStringList) "XhoI" "ctcgag" 2 0 1 1040 0 0 "")) 
COMMENT       (CObList 
COMMENT        #8=(CFSignal (CObList) "Kan" 4 0 1 1424 2239 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(739..1554)") 
COMMENT        #9=(CFSignal (CObList) "lacI" 4 0 1 3292 4374 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(2607..3689)") 
COMMENT        #10=(CFSignal (CObList) "T7 Promoter" 30 0 0 4498 4514 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "3813..3829") 
COMMENT        #11=(CFSignal (CObList) "lacO" 31 0 0 5 25 0 (CStringList) (CStringList) 
COMMENT             1 1 1 1 "5..25") 
COMMENT        #12=(CFSignal (CObList) "RBS-1" 32 0 0 58 63 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "58..63") 
COMMENT        #13=(CFSignal (CObList) "T7 Terminator" 43 0 0 1147 1194 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "462..509") 
COMMENT        #14=(CFSignal (CObList) "S-Tag" 31 0 0 1051 1095 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "366..410") 
COMMENT        #15=(CFSignal (CObList) "lacO" 31 0 0 575 595 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "235..255") 
COMMENT        #16=(CFSignal (CObList) "T7 promoter" 30 0 0 554 570 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "214..230") 
COMMENT        #17=(CFSignal (CObList) "RBS" 32 0 0 626 631 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "286..291") 
COMMENT        #18=(CFSignal (CObList) "RNA II" 53 0 1 2347 3045 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(1662..2360)") 
COMMENT        #19=(CFSignal (CObList) "RNA I" 53 0 0 2936 3042 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "2251..2357") 
COMMENT        #20=(CFSignal (CObList) "RSF ori - RSF1030 Homology" 33 0 0 2347 3096 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "1662..2411") 
COMMENT        #21=(CFSignal (CObList) "-10 RNA I" 47 0 0 2924 2929 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "2239..2244") 
COMMENT        #22=(CFSignal (CObList) "-35 RNA I" 48 0 0 2900 2905 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "2215..2220") 
COMMENT        #23=(CFSignal (CObList) "RBS" 32 0 1 2245 2249 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(1560..1564)") 
COMMENT        #24=(CFSignal (CObList) "-35 Ap" 48 0 1 2304 2309 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(1619..1624)") 
COMMENT        #25=(CFSignal (CObList) "-10 Ap" 47 0 1 2281 2287 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(1596..1602)") 
COMMENT        #26=(CFSignal (CObList) "Ivy" 4 0 0 71 481 0 (CStringList) (CStringList) 
COMMENT             1 1 1 1 "") 
COMMENT        #27=(CFSignal (CObList) "hLYS" 4 0 0 640 1032 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "")) (CObList) (CObList) (CObList) (CObList) 
COMMENT       (CObList) (CObList) 
COMMENT       (CTextView 0 
COMMENT        #28=(CGroupPar (CParagraph 0 (0 0) 1 2 0 0 180) 
COMMENT             (CObjectList 
COMMENT              #29=(CRefLinePar 
COMMENT                   (CLinePar (CParagraph 0 (0 0) 0 2 0 0 233) 
COMMENT                    "pRSF-Ivy-his-hLYZ" 2) 5 "" 0 4) 
COMMENT              #30=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 1 (0 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #31=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "DNA Plasmid 'pRSF-Ivy-his-hLYZ'" 1) 
COMMENT                     #32=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "pRSFDuet-1/Kan." 1) 
COMMENT                     #33=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Currently local object. Original author: EMD Biosciences Inc." 
COMMENT                          1) 
COMMENT                     #34=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Created: 02/14/13 06:19PM" 1) 
COMMENT                     #35=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Last Modified: 02/14/13 06:40PM" 1) 
COMMENT                     #36=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 4514 bp" 1) 
COMMENT                     #37=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "storage type: Basic" 1) 
COMMENT                     #38=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "form: Circular" 1))) "General Description") 
COMMENT              #39=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 2 (0 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #40=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Original Source Database: GenBank" 1) 
COMMENT                     #41=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Modification Date in the Original DB: 29-JUL-2003" 1))) 
COMMENT                   "Standard Fields") 
COMMENT              #42=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 5 (0 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
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COMMENT                          "EMD Biosciences Inc." 1) 
COMMENT                     #44=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "TEL: 608-442-4000  " 1) 
COMMENT                     #45=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "EMAIL: <a href=\"mailto:novagen@emdbiosciences.com\">novagen@emdbiosciences.com</a>" 
COMMENT                          1))) "Original Author") 
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COMMENT                   0 0) 
COMMENT              #47=(CFolderPar 
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COMMENT                   "Annotations") 
COMMENT              #48=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 12 (6 0) 1 2 0 0 178) 
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COMMENT                     #49=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 4 (7 4 0) 1 2 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #50=(CFolderPar 
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COMMENT                                  (CParagraph 71 (3 #26# 0) 1 2 2 0 327) 
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COMMENT                                   #51=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 71    End: 481 " 1) 
COMMENT                                   #52=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "E. coli Ivy gene, no leader, N-terminal MetGly residues, and C-terminal hexahistidine tag." 
COMMENT                                        1))) "Ivy") 
COMMENT                            #53=(CFolderPar 
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COMMENT                                  (CParagraph 640 (3 #27# 0) 1 2 2 0 327) 
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COMMENT                                        "Start: 640   End: 1032" 1) 
COMMENT                                   #55=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Human lysozyme gene, no leader, N-terminal methionine, dual stop codon" 
COMMENT                                        1))) "hLYS") 
COMMENT                            #56=(CFolderPar 
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COMMENT                                  (CParagraph 1424 (3 #8# 0) 1 2 2 0 327) 
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COMMENT                                   #57=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 1424  End: 2239 (Complementary)" 
COMMENT                                        1) 
COMMENT                                   #58=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #59=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   complement(739..1554)" 1))) "Kan") 
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COMMENT                                        "Start: 3292  End: 4374 (Complementary)" 
COMMENT                                        1) 
COMMENT                                   #62=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Wildtype lacI gene" 1) 
COMMENT                                   #63=(CLinePar 
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COMMENT                                   #64=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   complement(2607..3689)" 1))) "lacI"))) 
COMMENT                          "CDS (4 total)") 
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COMMENT                                        "Start: 554   End: 570 " 1) 
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COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #69=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   214..230" 1))) "T7 promoter") 
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COMMENT                                        "Start: 4498  End: 4514" 1) 
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COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #73=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   3813..3829" 1))) "T7 Promoter"))) 
COMMENT                          "Promoter Prokaryotic (2 total)") 
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COMMENT                                        "Start: 5     End: 25  " 1) 
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COMMENT                                   #78=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   5..25" 1))) "lacO") 
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COMMENT                                   #82=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   235..255" 1))) "lacO") 
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COMMENT                                        "Start: 1051  End: 1095" 1) 
COMMENT                                   #85=(CLinePar 
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COMMENT                                   #86=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   366..410" 1))) "S-Tag"))) 
COMMENT                          "Protein Binding Site (3 total)") 
COMMENT                     #87=(CFolderPar 
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COMMENT                                 (CGroupPar 
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COMMENT                                        "Start: 58    End: 63  " 1) 
COMMENT                                   #90=(CLinePar 
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COMMENT                                   #91=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   58..63" 1))) "RBS-1") 
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COMMENT                                        "Start: 2245  End: 2249 (Complementary)" 
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COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   complement(1560..1564)" 1))) "RBS"))) 
COMMENT                          "RBS (3 total)") 
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COMMENT                                   "RSF ori - RSF1030 Homology"))) 
COMMENT                           "Replication Origin (1 total)") 
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COMMENT                                           "   2239..2244" 1))) "-10 RNA I"))) 
COMMENT                           "-10 Signal (2 total)") 
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COMMENT                                           "   2215..2220" 1))) "-35 RNA I"))) 
COMMENT                           "-35 Signal (2 total)") 
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COMMENT                                           "   complement(1662..2360)" 1))) 
COMMENT                                   "RNA II") 
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COMMENT                                           "   2251..2357" 1))) "RNA I"))) 
COMMENT                           "RNA - Misc. (2 total)"))) "Feature Map") 
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COMMENT                                            " N1: 1040 " 1) 
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FEATURES             Location/Qualifiers
     CDS             complement(1424..2239)
                     /vntifkey="4"
                     /label=Kan
     CDS             complement(3292..4374)
                     /vntifkey="4"
                     /label=lacI
                     /note="Wildtype lacI gene"
     promoter        4498..4514
                     /vntifkey="30"
                     /label=T7\Promoter
     protein_bind    5..25
                     /vntifkey="31"
                     /label=lacO
     RBS             58..63
                     /vntifkey="32"
                     /label=RBS-1
     terminator      1147..1194
                     /vntifkey="43"
                     /label=T7\Terminator
     protein_bind    1051..1095
                     /vntifkey="31"
                     /label=S-Tag
     protein_bind    575..595
                     /vntifkey="31"
                     /label=lacO
     promoter        554..570
                     /vntifkey="30"
                     /label=T7\promoter
     RBS             626..631
                     /vntifkey="32"
                     /label=RBS
     misc_RNA        complement(2347..3045)
                     /vntifkey="53"
                     /label=RNA\II
     misc_RNA        2936..3042
                     /vntifkey="53"
                     /label=RNA\I
     rep_origin      2347..3096
                     /vntifkey="33"
                     /label=RSF\ori\-\RSF1030\Homology
     -10_signal      2924..2929
                     /vntifkey="47"
                     /label=-10\RNA\I
     -35_signal      2900..2905
                     /vntifkey="48"
                     /label=-35\RNA\I
     RBS             complement(2245..2249)
                     /vntifkey="32"
                     /label=RBS
     -35_signal      complement(2304..2309)
                     /vntifkey="48"
                     /label=-35\Ap
     -10_signal      complement(2281..2287)
                     /vntifkey="47"
                     /label=-10\Ap
     CDS             71..481
                     /vntifkey="4"
                     /label=Ivy
                     /note="E. coli Ivy gene, no leader, N-terminal MetGly residues, and C-terminal hexahistidine tag."
     CDS             640..1032
                     /vntifkey="4"
                     /label=hLYS
                     /note="Human lysozyme gene, no leader, N-terminal methionine, dual stop codon"
BASE COUNT     1183 a      1121 c      1098 g      1112 t 
ORIGIN
        1 ggggaattgt gagcggataa caattcccct gtagaaataa ttttgtttaa ctttaataag 
       61 gagatatacc atggggcagg atgatttaac cattagcagc cttgcaaagg gcgaaaccac 
      121 caaagctgca tttaatcaga tggtacaagg gcataagctg cctgcctggg tgatgaaagg 
      181 cggtacttat actcccgcac aaaccgtaac gttgggagat gagacgtatc aggtgatgag 
      241 cgcgtgcaaa ccgcatgact gtggctcgca acgtatcgct gtgatgtggt ccgagaaatc 
      301 taatcagatg acggggctgt tctcgactat tgatgagaaa acgtcgcaag agaaactcac 
      361 ctggctgaat gtgaacgatg cgctttcgat tgatggtaaa acggtgttgt tcgcggcgtt 
      421 gaccggcagc ctggaaaacc atccggatgg ctttaatttt aaacatcacc atcatcacca 
      481 ctaagcttgc ggccgcataa tgcttaagtc gaacagaaag taatcgtatt gtacacggcc 
      541 gcataatcga aattaatacg actcactata ggggaattgt gagcggataa caattcccca 
      601 tcttagtata ttagttaagt ataagaagga gatatacata tgaaggtctt tgaaaggtgt 
      661 gagttggcca gaactctgaa aagattggga atggatggct acaggggaat cagcctagca 
      721 aactggatgt gtttggccaa atgggagagt ggttacaaca cacgagctac aaactacaat 
      781 gctggagaca gaagcactga ttatgggata tttcagatca atagccgcta ctggtgtaat 
      841 gatggcaaaa ccccaggagc agttaatgcc tgtcatttat cctgcagtgc tttgctgcaa 
      901 gataacatcg ctgatgctgt agcttgtgca aagagggttg tccgtgatcc acaaggcatt 
      961 agagcatggg tggcatggag aaatcgttgt caaaacagag atgtccgtca gtatgttcaa 
     1021 ggttgtggag tgtaataact cgagtctggt aaagaaaccg ctgctgcgaa atttgaacgc 
     1081 cagcacatgg actcgtctac tagcgcagct taattaacct aggctgctgc caccgctgag 
     1141 caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa 
     1201 cctcaggcat ttgagaagca cacggtcaca ctgcttccgg tagtcaataa accggtaaac 
     1261 cagcaataga cataagcggc tatttaacga ccctgccctg aaccgacgac aagctgacga 
     1321 ccgggtctcc gcaagtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt 
     1381 tttctaaata cattcaaata tgtatccgct catgaattaa ttcttagaaa aactcatcga 
     1441 gcatcaaatg aaactgcaat ttattcatat caggattatc aataccatat ttttgaaaaa 
     1501 gccgtttctg taatgaagga gaaaactcac cgaggcagtt ccataggatg gcaagatcct 
     1561 ggtatcggtc tgcgattccg actcgtccaa catcaataca acctattaat ttcccctcgt 
     1621 caaaaataag gttatcaagt gagaaatcac catgagtgac gactgaatcc ggtgagaatg 
     1681 gcaaaagttt atgcatttct ttccagactt gttcaacagg ccagccatta cgctcgtcat 
     1741 caaaatcact cgcatcaacc aaaccgttat tcattcgtga ttgcgcctga gcgagacgaa 
     1801 atacgcggtc gctgttaaaa ggacaattac aaacaggaat cgaatgcaac cggcgcagga 
     1861 acactgccag cgcatcaaca atattttcac ctgaatcagg atattcttct aatacctgga 
     1921 atgctgtttt cccggggatc gcagtggtga gtaaccatgc atcatcagga gtacggataa 
     1981 aatgcttgat ggtcggaaga ggcataaatt ccgtcagcca gtttagtctg accatctcat 
     2041 ctgtaacatc attggcaacg ctacctttgc catgtttcag aaacaactct ggcgcatcgg 
     2101 gcttcccata caatcgatag attgtcgcac ctgattgccc gacattatcg cgagcccatt 
     2161 tatacccata taaatcagca tccatgttgg aatttaatcg cggcctagag caagacgttt 
     2221 cccgttgaat atggctcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt 
     2281 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggcatgc 
     2341 agcgctcttc cgcttcctcg ctcactgact cgctacgctc ggtcgttcga ctgcggcgag 
     2401 cggtgtcagc tcactcaaaa gcggtaatac ggttatccac agaatcaggg gataaagccg 
     2461 gaaagaacat gtgagcaaaa agcaaagcac cggaagaagc caacgccgca ggcgtttttc 
     2521 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagcca gaggtggcga 
     2581 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct 
     2641 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg 
     2701 gcgctttctc atagctcacg ctgttggtat ctcagttcgg tgtaggtcgt tcgctccaag 
     2761 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat 
     2821 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cattggtaac 
     2881 tgatttagag gactttgtct tgaagttatg cacctgttaa ggctaaactg aaagaacaga 
     2941 ttttggtgag tgcggtcctc caacccactt accttggttc aaagagttgg tagctcagcg 
     3001 aaccttgaga aaaccaccgt tggtagcggt ggtttttctt tatttatgag atgatgaatc 
     3061 aatcggtcta tcaagtcaac gaacagctat tccgttactc tagatttcag tgcaatttat 
     3121 ctcttcaaat gtagcacctg aagtcagccc catacgatat aagttgtaat tctcatgtta 
     3181 gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt 
     3241 cgagatcccg gtgcctaatg agtgagctaa cttacattaa ttgcgttgcg ctcactgccc 
     3301 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg 
     3361 agaggcggtt tgcgtattgg gcgccagggt ggtttttctt ttcaccagtg agacgggcaa 
     3421 cagctgattg cccttcaccg cctggccctg agagagttgc agcaagcggt ccacgctggt 
     3481 ttgccccagc aggcgaaaat cctgtttgat ggtggttaac ggcgggatat aacatgagct 
     3541 gtcttcggta tcgtcgtatc ccactaccga gatgtccgca ccaacgcgca gcccggactc 
     3601 ggtaatggcg cgcattgcgc ccagcgccat ctgatcgttg gcaaccagca tcgcagtggg 
     3661 aacgatgccc tcattcagca tttgcatggt ttgttgaaaa ccggacatgg cactccagtc 
     3721 gccttcccgt tccgctatcg gctgaatttg attgcgagtg agatatttat gccagccagc 
     3781 cagacgcaga cgcgccgaga cagaacttaa tgggcccgct aacagcgcga tttgctggtg 
     3841 acccaatgcg accagatgct ccacgcccag tcgcgtaccg tcttcatggg agaaaataat 
     3901 actgttgatg ggtgtctggt cagagacatc aagaaataac gccggaacat tagtgcaggc 
     3961 agcttccaca gcaatggcat cctggtcatc cagcggatag ttaatgatca gcccactgac 
     4021 gcgttgcgcg agaagattgt gcaccgccgc tttacaggct tcgacgccgc ttcgttctac 
     4081 catcgacacc accacgctgg cacccagttg atcggcgcga gatttaatcg ccgcgacaat 
     4141 ttgcgacggc gcgtgcaggg ccagactgga ggtggcaacg ccaatcagca acgactgttt 
     4201 gcccgccagt tgttgtgcca cgcggttggg aatgtaattc agctccgcca tcgccgcttc 
     4261 cactttttcc cgcgttttcg cagaaacgtg gctggcctgg ttcaccacgc gggaaacggt 
     4321 ctgataagag acaccggcat actctgcgac atcgtataac gttactggtt tcacattcac 
     4381 caccctgaat tgactctctt ccgggcgcta tcatgccata ccgcgaaagg ttttgcgcca 
     4441 ttcgatggtg tccgggatct cgacgctctc ccttatgcga ctcctgcatt aggaaattaa 
     4501 tacgactcac tata 
//
