LOCUS       pACYCDuet-1             5608 bp    DNA     circular     22-AUG-2002
DEFINITION  pACYCDUET-1.
SOURCE      
  ORGANISM  
COMMENT     http://www.informaxinc.com/
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|275403831|
COMMENT     VNTDBDATE|275677222|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pACYCDuet-1|
COMMENT     VNTAUTHORNAME|Bob Novy|
COMMENT     VNTREPLTYPE|Plasmid
COMMENT     VNTEXTCHREPL|Bacteria
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
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COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
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COMMENT        #2=(CRSite (CStringList) "BamHI" "ggatcc" 2 0 0 0 "") 
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COMMENT                          "Foreign object. Author: Bob Novy. Original author: Bob Novy" 
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FEATURES             Location/Qualifiers
     rep_origin      3350..4262
                     /vntifkey="33"
                     /label=P15A\ori
     CDS             complement(4386..5468)
                     /vntifkey="4"
                     /label=lacI
                     /note="lacI gene"
     promoter        5592..5608
                     /vntifkey="30"
                     /label=T7\Promoter
     protein_bind    5..25
                     /vntifkey="31"
                     /label=lacO
     CDS             complement(2329..2988)
                     /vntifkey="4"
                     /label=CAT
     RBS             58..59
                     /vntifkey="32"
                     /label=RBS-1
     terminator      2062..2109
                     /vntifkey="43"
                     /label=T7\Terminator
     protein_bind    1966..2010
                     /vntifkey="31"
                     /label=S-Tag
     CDS             1954..2028
                     /vntifkey="4"
                     /label=MCS-2\ORF
     protein_bind    590..610
                     /vntifkey="31"
                     /label=lacO
     promoter        569..585
                     /vntifkey="30"
                     /label=T7\promoter
     primer          641..646
                     primer          3949..3968
                     primer          189..208
                     primer          complement(189..208)
                     primer          complement(448..466)
                     /vntifkey="27"
                     /label=T7\Terminator\Primer\#69337-3
     misc_feature    71..499
                     /vntifkey="21"
                     /label=Skp
     misc_feature    655..1953
                     /vntifkey="21"
                     /label=Trigger\Factor
BASE COUNT     1467 a      1392 c      1438 g      1311 t 
ORIGIN
        1 ggggaattgt gagcggataa caattcccct gtagaaataa ttttgtttaa ctttaataag 
       61 gagatatacc atggctgaca aaattgcaat cgtcaacatg ggcagcctgt tccagcaggt 
      121 agcgcagaaa accggtgttt ctaacacgct ggaaaatgag ttcaaaggcc gtgccagcga 
      181 actgcagcgt atggaaaccg atctgcaggc taaaatgaaa aagctgcagt ccatgaaagc 
      241 gggcagcgat cgcactaagc tggaaaaaga cgtgatggct cagcgccaga cttttgctca 
      301 gaaagcgcag gcttttgagc aggatcgcgc acgtcgttcc aacgaagaac gcggcaaact 
      361 ggttactcgt atccagactg ctgtgaaatc cgttgccaac agccaggata tcgatctggt 
      421 tgttgatgca aacgccgttg cttacaacag cagcgatgta aaagacatca ctgccgacgt 
      481 actgaaacag gttaaataag cttgcggccg cataatgctt aagtcgaaca gaaagtaatc 
      541 gtattgtaca cggccgcata atcgaaatta atacgactca ctatagggga attgtgagcg 
      601 gataacaatt ccccatctta gtatattagt taagtataag aaggagatat acatatgcaa 
      661 gtttcagttg aaaccactca aggccttggc cgccgtgtaa cgattactgt cgctgctgac 
      721 agcatcgaga ccgctgttaa aagcgagctg gtcaacgttg cgaaaaaagt acgtattgac 
      781 ggcttccgca aaggcaaagt gccaatgaat atcgttgctc agcgttatgg cgcgtctgta 
      841 cgccaggacg ttctgggtga cctgatgagc cgtaacttca ttgacgccat cattaaagaa 
      901 aaaatcaatc cggctggcgc accgacttat gttccgggcg aatacaagct gggtgaagac 
      961 ttcacttact ctgtagagtt tgaagtttat ccggaagttg aactgcaggg tctggaagcg 
     1021 atcgaagttg aaaaaccgat cgttgaagtg accgacgctg acgttgacgg catgctggat 
     1081 actctgcgta aacagcaggc gacctggaaa gaaaaagacg gcgctgttga agcagaagac 
     1141 cgcgtaacca tcgacttcac cggttctgta gacggcgaag agttcgaagg cggtaaagcg 
     1201 tctgatttcg tactggcgat gggccagggt cgtatgatcc cgggctttga agacggtatc 
     1261 aaaggccaca aagctggcga agagttcacc atcgacgtga ccttcccgga agaataccac 
     1321 gcagaaagcc tgaaaggtaa agcagcgaaa ttcgctatca acctgaagaa agttgaagag 
     1381 cgtgaactgc cggaactgac tgcagaattc atcaaacgtt tcggcgttga agatggttcc 
     1441 gtagaaggtc tgcgcgctga agtgcgtaaa aacatggagc gcgagctgaa gagcgccatc 
     1501 cgtaaccgcg ttaagtctca ggcgatcgaa ggtctggtaa aagctaacga catcgacgta 
     1561 ccggctgcgc tgatcgacag cgaaatcgac gttctgcgtc gccaggctgc acagcgtttc 
     1621 ggtggcaacg aaaaacaagc tctggaactg ccgcgcgaac tgttcgaaga acaggctaaa 
     1681 cgccgcgtag ttgttggcct gctgctgggc gaagttatcc gcaccaacga gctgaaagct 
     1741 gacgaggagc gcgtgaaagg cctgatcgaa gagatggctt ctgcgtacga agatccgaaa 
     1801 gaagttatcg agttctacag caaaaacaaa gaactgatgg acaacatgcg caatgttgct 
     1861 ctggaagaac aggctgttga agctgtactg gcgaaagcga aagtgactga aaaagaaacc 
     1921 actttcaacg agctgatgaa ccagcaggcg taactcgagt ctggtaaaga aaccgctgct 
     1981 gcgaaatttg aacgccagca catggactcg tctactagcg cagcttaatt aacctaggct 
     2041 gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg 
     2101 ggttttttgc tgaaacctca ggcatttgag aagcacacgg tcacactgct tccggtagtc 
     2161 aataaaccgg taaaccagca atagacataa gcggctattt aacgaccctg ccctgaaccg 
     2221 acgaccgggt cgaatttgct ttcgaatttc tgccattcat ccgcttatta tcacttattc 
     2281 aggcgtagca ccaggcgttt aagggcacca ataactgcct taaaaaaatt acgccccgcc 
     2341 ctgccactca tcgcagtact gttgtaattc attaagcatt ctgccgacat ggaagccatc 
     2401 acagacggca tgatgaacct gaatcgccag cggcatcagc accttgtcgc cttgcgtata 
     2461 atatttgccc atagtgaaaa cgggggcgaa gaagttgtcc atattggcca cgtttaaatc 
     2521 aaaactggtg aaactcaccc agggattggc tgagacgaaa aacatattct caataaaccc 
     2581 tttagggaaa taggccaggt tttcaccgta acacgccaca tcttgcgaat atatgtgtag 
     2641 aaactgccgg aaatcgtcgt ggtattcact ccagagcgat gaaaacgttt cagtttgctc 
     2701 atggaaaacg gtgtaacaag ggtgaacact atcccatatc accagctcac cgtctttcat 
     2761 tgccatacgg aactccggat gagcattcat caggcgggca agaatgtgaa taaaggccgg 
     2821 ataaaacttg tgcttatttt tctttacggt ctttaaaaag gccgtaatat ccagctgaac 
     2881 ggtctggtta taggtacatt gagcaactga ctgaaatgcc tcaaaatgtt ctttacgatg 
     2941 ccattgggat atatcaacgg tggtatatcc agtgattttt ttctccattt tagcttcctt 
     3001 agctcctgaa aatctcgata actcaaaaaa tacgcccggt agtgatctta tttcattatg 
     3061 gtgaaagttg gaacctctta cgtgccgatc aacgtctcat tttcgccaaa agttggccca 
     3121 gggcttcccg gtatcaacag ggacaccagg atttatttat tctgcgaagt gatcttccgt 
     3181 cacaggtatt tattcggcgc aaagtgcgtc gggtgatgct gccaacttac tgatttagtg 
     3241 tatgatggtg tttttgaggt gctccagtgg cttctgtttc tatcagctgt ccctcctgtt 
     3301 cagctactga cggggtggtg cgtaacggca aaagcaccgc cggacatcag cgctagcgga 
     3361 gtgtatactg gcttactatg ttggcactga tgagggtgtc agtgaagtgc ttcatgtggc 
     3421 aggagaaaaa aggctgcacc ggtgcgtcag cagaatatgt gatacaggat atattccgct 
     3481 tcctcgctca ctgactcgct acgctcggtc gttcgactgc ggcgagcgga aatggcttac 
     3541 gaacggggcg gagatttcct ggaagatgcc aggaagatac ttaacaggga agtgagaggg 
     3601 ccgcggcaaa gccgtttttc cataggctcc gcccccctga caagcatcac gaaatctgac 
     3661 gctcaaatca gtggtggcga aacccgacag gactataaag ataccaggcg tttcccctgg 
     3721 cggctccctc gtgcgctctc ctgttcctgc ctttcggttt accggtgtca ttccgctgtt 
     3781 atggccgcgt ttgtctcatt ccacgcctga cactcagttc cgggtaggca gttcgctcca 
     3841 agctggactg tatgcacgaa ccccccgttc agtccgaccg ctgcgcctta tccggtaact 
     3901 atcgtcttga gtccaacccg gaaagacatg caaaagcacc actggcagca gccactggta 
     3961 attgatttag aggagttagt cttgaagtca tgcgccggtt aaggctaaac tgaaaggaca 
     4021 agttttggtg actgcgctcc tccaagccag ttacctcggt tcaaagagtt ggtagctcag 
     4081 agaaccttcg aaaaaccgcc ctgcaaggcg gttttttcgt tttcagagca agagattacg 
     4141 cgcagaccaa aacgatctca agaagatcat cttattaatc agataaaata tttctagatt 
     4201 tcagtgcaat ttatctcttc aaatgtagca cctgaagtca gccccatacg atataagttg 
     4261 taattctcat gttagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc 
     4321 tcaagggcat cggtcgagat cccggtgcct aatgagtgag ctaacttaca ttaattgcgt 
     4381 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg 
     4441 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgcca gggtggtttt tcttttcacc 
     4501 agtgagacgg gcaacagctg attgcccttc accgcctggc cctgagagag ttgcagcaag 
     4561 cggtccacgc tggtttgccc cagcaggcga aaatcctgtt tgatggtggt taacggcggg 
     4621 atataacatg agctgtcttc ggtatcgtcg tatcccacta ccgagatgtc cgcaccaacg 
     4681 cgcagcccgg actcggtaat ggcgcgcatt gcgcccagcg ccatctgatc gttggcaacc 
     4741 agcatcgcag tgggaacgat gccctcattc agcatttgca tggtttgttg aaaaccggac 
     4801 atggcactcc agtcgccttc ccgttccgct atcggctgaa tttgattgcg agtgagatat 
     4861 ttatgccagc cagccagacg cagacgcgcc gagacagaac ttaatgggcc cgctaacagc 
     4921 gcgatttgct ggtgacccaa tgcgaccaga tgctccacgc ccagtcgcgt accgtcttca 
     4981 tgggagaaaa taatactgtt gatgggtgtc tggtcagaga catcaagaaa taacgccgga 
     5041 acattagtgc aggcagcttc cacagcaatg gcatcctggt catccagcgg atagttaatg 
     5101 atcagcccac tgacgcgttg cgcgagaaga ttgtgcaccg ccgctttaca ggcttcgacg 
     5161 ccgcttcgtt ctaccatcga caccaccacg ctggcaccca gttgatcggc gcgagattta 
     5221 atcgccgcga caatttgcga cggcgcgtgc agggccagac tggaggtggc aacgccaatc 
     5281 agcaacgact gtttgcccgc cagttgttgt gccacgcggt tgggaatgta attcagctcc 
     5341 gccatcgccg cttccacttt ttcccgcgtt ttcgcagaaa cgtggctggc ctggttcacc 
     5401 acgcgggaaa cggtctgata agagacaccg gcatactctg cgacatcgta taacgttact 
     5461 ggtttcacat tcaccaccct gaattgactc tcttccgggc gctatcatgc cataccgcga 
     5521 aaggttttgc gccattcgat ggtgtccggg atctcgacgc tctcccttat gcgactcctg 
     5581 cattaggaaa ttaatacgac tcactata 
//
