LOCUS       pACYCDuet-1        6293 bp    DNA     circular     22-AUG-2002
DEFINITION  pACYCDUET-1.
SOURCE      
  ORGANISM  
COMMENT     http://www.informaxinc.com/
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|275403831|
COMMENT     VNTDBDATE|275677222|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pACYCDuet-1|
COMMENT     VNTAUTHORNAME|Bob Novy|
COMMENT     VNTREPLTYPE|Plasmid
COMMENT     VNTEXTCHREPL|Bacteria
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pACYCDuet-1" 0 6293 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
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COMMENT        (CStringList "taa" "tga" "tag") (CObList) 1 "{(0,1),2}" 0 0 "" 0 
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COMMENT        (CProteinMotifSearchObject 70 20 1 1 1 1 0 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 0 4294967295 1 6293 0 0 0 0 0 0 0 0) (CStringList) 
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COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
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COMMENT        #1=(CRSite (CStringList) "AvaI" "cycgrg" 2 0 1 2640 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "BamHI" "ggatcc" 2 0 1 2474 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "ClaI" "atcgat" 3 0 2 412 0 1990 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 0 0 "") 
COMMENT        #5=(CRSite (CStringList) "HindIII" "aagctt" 2 0 1 499 0 0 "") 
COMMENT        #6=(CRSite (CStringList) "NcoI" "ccatgg" 2 0 1 70 0 0 "") 
COMMENT        #7=(CRSite (CStringList) "PstI" "ctgcag" 6 0 10 187 0 208 0 229 0 748 0 
COMMENT            1247 0 1373 0 1543 0 1861 0 2089 0 2506 0 0 "") 
COMMENT        #8=(CRSite (CStringList) "SmaI" "" 859056160 673214562 0 0 "") 
COMMENT        #9=(CRSite (CStringList) "XmaI" "" 695231008 1646277943 0 0 "") 
COMMENT        #10=(CRSite (CStringList) "NdeI" "catatg" 3 0 1 654 0 0 "") 
COMMENT        #11=(CRSite (CStringList) "XhoI" "" 0 11822 0 0 "")) 
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COMMENT        #13=(CFSignal (CObList) "lacI" 4 0 1 5071 6153 0 (CStringList) 
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COMMENT        #14=(CFSignal (CObList) "T7 Promoter" 30 0 0 6277 6293 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "5592..5608") 
COMMENT        #15=(CFSignal (CObList) "lacO" 31 0 0 5 25 0 (CStringList) (CStringList) 
COMMENT             1 1 1 1 "5..25") 
COMMENT        #16=(CFSignal (CObList) "CAT" 4 0 1 3014 3673 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(2329..2988)") 
COMMENT        #17=(CFSignal (CObList) "RBS-1" 32 0 0 58 59 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "58..59") 
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COMMENT             (CStringList) 1 1 1 1 "2062..2109") 
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COMMENT        #20=(CFSignal (CObList) "MCS-2 ORF" 4 0 0 2639 2713 0 (CStringList) 
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COMMENT        #21=(CFSignal (CObList) "lacO" 31 0 0 590 610 0 (CStringList) 
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COMMENT        #22=(CFSignal (CObList) "T7 promoter" 30 0 0 569 585 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "569..585") 
COMMENT        #23=(CFSignal (CObList) "Skp" 21 0 0 71 499 0 (CStringList) 
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COMMENT        #24=(CFSignal (CObList) "GroES" 21 0 0 655 948 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
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COMMENT                          "Foreign object. Author: Bob Novy. Original author: Bob Novy" 
COMMENT                          1) 
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COMMENT                          "Created: 08/19/02 01:03PM" 1) 
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COMMENT                          "Last Modified: 08/22/02 05:00PM" 1) 
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FEATURES             Location/Qualifiers
     rep_origin      4035..4947
                     /vntifkey="33"
                     /label=P15A\ori
     CDS             complement(5071..6153)
                     /vntifkey="4"
                     /label=lacI
                     /note="lacI gene"
     promoter        6277..6293
                     /vntifkey="30"
                     /label=T7\Promoter
     protein_bind    5..25
                     /vntifkey="31"
                     /label=lacO
     CDS             complement(3014..3673)
                     /vntifkey="4"
                     /label=CAT
     RBS             58..59
                     /vntifkey="32"
                     /label=RBS-1
     terminator      2747..2794
                     /vntifkey="43"
                     /label=T7\Terminator
     protein_bind    2651..2695
                     /vntifkey="31"
                     /label=S-Tag
     CDS             2639..2713
                     /vntifkey="4"
                     /label=MCS-2\ORF
     protein_bind    590..610
                     /vntifkey="31"
                     /label=lacO
     promoter        569..585
                     /vntifkey="30"
                     /label=T7\promoter
     primer          641..646primer          3949..3968primer          189..208primer          complement(189..208)primer          complement(448..466)
                     /vntifkey="27"
                     /label=T7\Terminator\Primer\#69337-3
     misc_feature    71..499
                     /vntifkey="21"
                     /label=Skp
     misc_feature    655..948
                     /vntifkey="21"
                     /label=GroES
     misc_feature    992..2638
                     /vntifkey="21"
                     /label=GroEL
BASE COUNT     1629 a      1557 c      1636 g      1471 t 
ORIGIN
        1 ggggaattgt gagcggataa caattcccct gtagaaataa ttttgtttaa ctttaataag 
       61 gagatatacc atggctgaca aaattgcaat cgtcaacatg ggcagcctgt tccagcaggt 
      121 agcgcagaaa accggtgttt ctaacacgct ggaaaatgag ttcaaaggcc gtgccagcga 
      181 actgcagcgt atggaaaccg atctgcaggc taaaatgaaa aagctgcagt ccatgaaagc 
      241 gggcagcgat cgcactaagc tggaaaaaga cgtgatggct cagcgccaga cttttgctca 
      301 gaaagcgcag gcttttgagc aggatcgcgc acgtcgttcc aacgaagaac gcggcaaact 
      361 ggttactcgt atccagactg ctgtgaaatc cgttgccaac agccaggata tcgatctggt 
      421 tgttgatgca aacgccgttg cttacaacag cagcgatgta aaagacatca ctgccgacgt 
      481 actgaaacag gttaaataag cttgcggccg cataatgctt aagtcgaaca gaaagtaatc 
      541 gtattgtaca cggccgcata atcgaaatta atacgactca ctatagggga attgtgagcg 
      601 gataacaatt ccccatctta gtatattagt taagtataag aaggagatat acatatgaat 
      661 attcgtccat tgcatgatcg cgtgatcgtc aagcgtaaag aagttgaaac taaatctgct 
      721 ggcggcatcg ttctgaccgg ctctgcagcg gctaaatcca cccgcggcga agtgctggct 
      781 gtcggcaatg gccgtatcct tgaaaatggc gaagtgaagc cgctggatgt gaaagttggc 
      841 gacatcgtta ttttcaacga tggctacggt gtgaaatctg agaagatcga caatgaagaa 
      901 gtgttgatca tgtccgaaag cgacattctg gcaattgttg aagcgtaatc cgcgcacgac 
      961 actgaacata cgaatttaag gaataaagat aatggcagct aaagacgtaa aattcggtaa 
     1021 cgacgctcgt gtgaaaatgc tgcgcggcgt aaacgtactg gcagatgcag tgaaagttac 
     1081 cctcggtcca aaaggccgta acgtagttct ggataaatct ttcggtgcac cgaccatcac 
     1141 caaagatggt gtttccgttg ctcgtgaaat cgaactggaa gacaagttcg aaaatatggg 
     1201 tgcgcagatg gtgaaagaag ttgcctctaa agcaaacgac gctgcaggcg acggtaccac 
     1261 cactgcaacc gtactggctc aggctatcat cactgaaggt ctgaaagctg ttgctgcggg 
     1321 catgaacccg atggacctga aacgtggtat cgacaaagcg gttaccgctg cagttgaaga 
     1381 actgaaagcg ctgtccgtac catgctctga ctctaaagcg attgctcagg ttggtaccat 
     1441 ctccgctaac tccgacgaaa ccgtaggtaa actgatcgct gaagcgatgg acaaagtcgg 
     1501 taaagaaggc gttatcaccg ttgaagacgg taccggtctg caggacgaac tggacgtggt 
     1561 tgaaggtatg cagttcgacc gtggctacct gtctccttac ttcatcaaca agccggaaac 
     1621 tggcgcagta gaactggaaa gcccgttcat cctgctggct gacaagaaaa tctccaacat 
     1681 ccgcgaaatg ctgccggttc tggaagctgt tgccaaagca ggcaaaccgc tgctgatcat 
     1741 cgctgaagat gtagaaggcg aagcgctggc aactctggtt gttaacacca tgcgtggcat 
     1801 cgtgaaagtc gctgcggtta aagcaccggg cttcggcgat cgtcgtaaag ctatgctgca 
     1861 ggatatcgca accctgactg gcggtaccgt gatctctgaa gagatcggta tggagctgga 
     1921 aaaagcaacc ctggaagacc tgggtcaggc taaacgtgtt gtgatcaaca aagacaccac 
     1981 cactatcatc gatggcgtgg gtgaagaagc tgcaatccag ggccgtgttg ctcagatccg 
     2041 tcagcagatt gaagaagcaa cttctgacta cgaccgtgaa aaactgcagg aacgcgtagc 
     2101 gaaactggca ggcggcgttg cagttatcaa agtgggtgct gctaccgaag ttgaaatgaa 
     2161 agagaaaaaa gcacgcgttg aagatgccct gcacgcgacc cgtgctgcgg tagaagaagg 
     2221 cgtggttgct ggtggtggtg ttgcgctgat ccgcgtagcg tctaaactgg ctgacctgcg 
     2281 tggtcagaac gaagaccaga acgtgggtat caaagttgca ctgcgtgcaa tggaagctcc 
     2341 gctgcgtcag atcgtattga actgcggcga agaaccgtct gttgttgcta acaccgttaa 
     2401 aggcggcgac ggcaactacg gttacaacgc agcaaccgaa gaatacggca acatgatcga 
     2461 catgggtatc ctggatccaa ccaaagtaac tcgttctgct ctgcagtacg cagcttctgt 
     2521 ggctggcctg atgatcacca ccgaatgcat ggttaccgac ctgccgaaaa acgatgcagc 
     2581 tgacttaggc gctgctggcg gtatgggcgg catgggtggc atgggcggca tgatgtaact 
     2641 cgagtctggt aaagaaaccg ctgctgcgaa atttgaacgc cagcacatgg actcgtctac 
     2701 tagcgcagct taattaacct aggctgctgc caccgctgag caataactag cataacccct 
     2761 tggggcctct aaacgggtct tgaggggttt tttgctgaaa cctcaggcat ttgagaagca 
     2821 cacggtcaca ctgcttccgg tagtcaataa accggtaaac cagcaataga cataagcggc 
     2881 tatttaacga ccctgccctg aaccgacgac cgggtcgaat ttgctttcga atttctgcca 
     2941 ttcatccgct tattatcact tattcaggcg tagcaccagg cgtttaaggg caccaataac 
     3001 tgccttaaaa aaattacgcc ccgccctgcc actcatcgca gtactgttgt aattcattaa 
     3061 gcattctgcc gacatggaag ccatcacaga cggcatgatg aacctgaatc gccagcggca 
     3121 tcagcacctt gtcgccttgc gtataatatt tgcccatagt gaaaacgggg gcgaagaagt 
     3181 tgtccatatt ggccacgttt aaatcaaaac tggtgaaact cacccaggga ttggctgaga 
     3241 cgaaaaacat attctcaata aaccctttag ggaaataggc caggttttca ccgtaacacg 
     3301 ccacatcttg cgaatatatg tgtagaaact gccggaaatc gtcgtggtat tcactccaga 
     3361 gcgatgaaaa cgtttcagtt tgctcatgga aaacggtgta acaagggtga acactatccc 
     3421 atatcaccag ctcaccgtct ttcattgcca tacggaactc cggatgagca ttcatcaggc 
     3481 gggcaagaat gtgaataaag gccggataaa acttgtgctt atttttcttt acggtcttta 
     3541 aaaaggccgt aatatccagc tgaacggtct ggttataggt acattgagca actgactgaa 
     3601 atgcctcaaa atgttcttta cgatgccatt gggatatatc aacggtggta tatccagtga 
     3661 tttttttctc cattttagct tccttagctc ctgaaaatct cgataactca aaaaatacgc 
     3721 ccggtagtga tcttatttca ttatggtgaa agttggaacc tcttacgtgc cgatcaacgt 
     3781 ctcattttcg ccaaaagttg gcccagggct tcccggtatc aacagggaca ccaggattta 
     3841 tttattctgc gaagtgatct tccgtcacag gtatttattc ggcgcaaagt gcgtcgggtg 
     3901 atgctgccaa cttactgatt tagtgtatga tggtgttttt gaggtgctcc agtggcttct 
     3961 gtttctatca gctgtccctc ctgttcagct actgacgggg tggtgcgtaa cggcaaaagc 
     4021 accgccggac atcagcgcta gcggagtgta tactggctta ctatgttggc actgatgagg 
     4081 gtgtcagtga agtgcttcat gtggcaggag aaaaaaggct gcaccggtgc gtcagcagaa 
     4141 tatgtgatac aggatatatt ccgcttcctc gctcactgac tcgctacgct cggtcgttcg 
     4201 actgcggcga gcggaaatgg cttacgaacg gggcggagat ttcctggaag atgccaggaa 
     4261 gatacttaac agggaagtga gagggccgcg gcaaagccgt ttttccatag gctccgcccc 
     4321 cctgacaagc atcacgaaat ctgacgctca aatcagtggt ggcgaaaccc gacaggacta 
     4381 taaagatacc aggcgtttcc cctggcggct ccctcgtgcg ctctcctgtt cctgcctttc 
     4441 ggtttaccgg tgtcattccg ctgttatggc cgcgtttgtc tcattccacg cctgacactc 
     4501 agttccgggt aggcagttcg ctccaagctg gactgtatgc acgaaccccc cgttcagtcc 
     4561 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggaaag acatgcaaaa 
     4621 gcaccactgg cagcagccac tggtaattga tttagaggag ttagtcttga agtcatgcgc 
     4681 cggttaaggc taaactgaaa ggacaagttt tggtgactgc gctcctccaa gccagttacc 
     4741 tcggttcaaa gagttggtag ctcagagaac cttcgaaaaa ccgccctgca aggcggtttt 
     4801 ttcgttttca gagcaagaga ttacgcgcag accaaaacga tctcaagaag atcatcttat 
     4861 taatcagata aaatatttct agatttcagt gcaatttatc tcttcaaatg tagcacctga 
     4921 agtcagcccc atacgatata agttgtaatt ctcatgttag tcatgccccg cgcccaccgg 
     4981 aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gagatcccgg tgcctaatga 
     5041 gtgagctaac ttacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg 
     5101 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg 
     5161 cgccagggtg gtttttcttt tcaccagtga gacgggcaac agctgattgc ccttcaccgc 
     5221 ctggccctga gagagttgca gcaagcggtc cacgctggtt tgccccagca ggcgaaaatc 
     5281 ctgtttgatg gtggttaacg gcgggatata acatgagctg tcttcggtat cgtcgtatcc 
     5341 cactaccgag atgtccgcac caacgcgcag cccggactcg gtaatggcgc gcattgcgcc 
     5401 cagcgccatc tgatcgttgg caaccagcat cgcagtggga acgatgccct cattcagcat 
     5461 ttgcatggtt tgttgaaaac cggacatggc actccagtcg ccttcccgtt ccgctatcgg 
     5521 ctgaatttga ttgcgagtga gatatttatg ccagccagcc agacgcagac gcgccgagac 
     5581 agaacttaat gggcccgcta acagcgcgat ttgctggtga cccaatgcga ccagatgctc 
     5641 cacgcccagt cgcgtaccgt cttcatggga gaaaataata ctgttgatgg gtgtctggtc 
     5701 agagacatca agaaataacg ccggaacatt agtgcaggca gcttccacag caatggcatc 
     5761 ctggtcatcc agcggatagt taatgatcag cccactgacg cgttgcgcga gaagattgtg 
     5821 caccgccgct ttacaggctt cgacgccgct tcgttctacc atcgacacca ccacgctggc 
     5881 acccagttga tcggcgcgag atttaatcgc cgcgacaatt tgcgacggcg cgtgcagggc 
     5941 cagactggag gtggcaacgc caatcagcaa cgactgtttg cccgccagtt gttgtgccac 
     6001 gcggttggga atgtaattca gctccgccat cgccgcttcc actttttccc gcgttttcgc 
     6061 agaaacgtgg ctggcctggt tcaccacgcg ggaaacggtc tgataagaga caccggcata 
     6121 ctctgcgaca tcgtataacg ttactggttt cacattcacc accctgaatt gactctcttc 
     6181 cgggcgctat catgccatac cgcgaaaggt tttgcgccat tcgatggtgt ccgggatctc 
     6241 gacgctctcc cttatgcgac tcctgcatta ggaaattaat acgactcact ata 
//
