LOCUS       pACYCDuet-1        7166 bp    DNA     circular     22-AUG-2002
DEFINITION  pACYCDUET-1.
SOURCE      
  ORGANISM  
COMMENT     http://www.informaxinc.com/
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|275403831|
COMMENT     VNTDBDATE|275677222|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pACYCDuet-1|
COMMENT     VNTAUTHORNAME|Bob Novy|
COMMENT     VNTREPLTYPE|Plasmid
COMMENT     VNTEXTCHREPL|Bacteria
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pACYCDuet-1" 0 7166 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
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COMMENT        (CProteinMotifSearchObject 70 20 1 1 1 1 0 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 0 4294967295 1 7166 0 0 0 0 0 0 0 0) (CStringList) 
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COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
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COMMENT        #1=(CRSite (CStringList) "AvaI" "cycgrg" 2 0 2 2798 0 3513 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "BamHI" "ggatcc" 2 0 1 1893 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "ClaI" "atcgat" 3 0 1 1409 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 1 2964 0 0 "") 
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COMMENT        #6=(CRSite (CStringList) "NcoI" "ccatgg" 2 0 1 70 0 0 "") 
COMMENT        #7=(CRSite (CStringList) "PstI" "ctgcag" 6 0 9 167 0 666 0 792 0 962 0 
COMMENT            1280 0 1508 0 1925 0 2566 0 2963 0 0 "") 
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COMMENT        #9=(CRSite (CStringList) "XmaI" "" 695231008 1072693248 0 0 "") 
COMMENT        #10=(CRSite (CStringList) "NdeI" "catatg" 3 0 1 2212 0 0 "") 
COMMENT        #11=(CRSite (CStringList) "XhoI" "" 0 14958 0 0 "")) 
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COMMENT             1 1 1 1 "5..25") 
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COMMENT        #17=(CFSignal (CObList) "RBS-1" 32 0 0 58 59 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "58..59") 
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COMMENT        #20=(CFSignal (CObList) "MCS-2 ORF" 4 0 0 3512 3586 0 (CStringList) 
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COMMENT        #22=(CFSignal (CObList) "T7 promoter" 30 0 0 2127 2143 0 (CStringList) 
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COMMENT                          "Foreign object. Author: Bob Novy. Original author: Bob Novy" 
COMMENT                          1) 
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COMMENT                          "Created: 08/19/02 01:03PM" 1) 
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COMMENT                          "Last Modified: 08/22/02 05:00PM" 1) 
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FEATURES             Location/Qualifiers
     rep_origin      4908..5820
                     /vntifkey="33"
                     /label=P15A\ori
     CDS             complement(5944..7026)
                     /vntifkey="4"
                     /label=lacI
                     /note="lacI gene"
     promoter        7150..7166
                     /vntifkey="30"
                     /label=T7\Promoter
     protein_bind    5..25
                     /vntifkey="31"
                     /label=lacO
     CDS             complement(3887..4546)
                     /vntifkey="4"
                     /label=CAT
     RBS             58..59
                     /vntifkey="32"
                     /label=RBS-1
     terminator      3620..3667
                     /vntifkey="43"
                     /label=T7\Terminator
     protein_bind    3524..3568
                     /vntifkey="31"
                     /label=S-Tag
     CDS             3512..3586
                     /vntifkey="4"
                     /label=MCS-2\ORF
     protein_bind    2148..2168
                     /vntifkey="31"
                     /label=lacO
     promoter        2127..2143
                     /vntifkey="30"
                     /label=T7\promoter
     primer          641..646primer          3949..3968primer          189..208primer          complement(189..208)primer          complement(448..466)
                     /vntifkey="27"
                     /label=T7\Terminator\Primer\#69337-3
     misc_feature    2213..3511
                     /vntifkey="21"
                     /label=Trigger\Factor
     misc_feature    71..367
                     /vntifkey="21"
                     /label=GroES
     misc_feature    411..2057
                     /vntifkey="21"
                     /label=GroEL
BASE COUNT     1871 a      1753 c      1896 g      1646 t 
ORIGIN
        1 ggggaattgt gagcggataa caattcccct gtagaaataa ttttgtttaa ctttaataag 
       61 gagatatacc atggggaata ttcgtccatt gcatgatcgc gtgatcgtca agcgtaaaga 
      121 agttgaaact aaatctgctg gcggcatcgt tctgaccggc tctgcagcgg ctaaatccac 
      181 ccgcggcgaa gtgctggctg tcggcaatgg ccgtatcctt gaaaatggcg aagtgaagcc 
      241 gctggatgtg aaagttggcg acatcgttat tttcaacgat ggctacggtg tgaaatctga 
      301 gaagatcgac aatgaagaag tgttgatcat gtccgaaagc gacattctgg caattgttga 
      361 agcgtaatcc gcgcacgaca ctgaacatac gaatttaagg aataaagata atggcagcta 
      421 aagacgtaaa attcggtaac gacgctcgtg tgaaaatgct gcgcggcgta aacgtactgg 
      481 cagatgcagt gaaagttacc ctcggtccaa aaggccgtaa cgtagttctg gataaatctt 
      541 tcggtgcacc gaccatcacc aaagatggtg tttccgttgc tcgtgaaatc gaactggaag 
      601 acaagttcga aaatatgggt gcgcagatgg tgaaagaagt tgcctctaaa gcaaacgacg 
      661 ctgcaggcga cggtaccacc actgcaaccg tactggctca ggctatcatc actgaaggtc 
      721 tgaaagctgt tgctgcgggc atgaacccga tggacctgaa acgtggtatc gacaaagcgg 
      781 ttaccgctgc agttgaagaa ctgaaagcgc tgtccgtacc atgctctgac tctaaagcga 
      841 ttgctcaggt tggtaccatc tccgctaact ccgacgaaac cgtaggtaaa ctgatcgctg 
      901 aagcgatgga caaagtcggt aaagaaggcg ttatcaccgt tgaagacggt accggtctgc 
      961 aggacgaact ggacgtggtt gaaggtatgc agttcgaccg tggctacctg tctccttact 
     1021 tcatcaacaa gccggaaact ggcgcagtag aactggaaag cccgttcatc ctgctggctg 
     1081 acaagaaaat ctccaacatc cgcgaaatgc tgccggttct ggaagctgtt gccaaagcag 
     1141 gcaaaccgct gctgatcatc gctgaagatg tagaaggcga agcgctggca actctggttg 
     1201 ttaacaccat gcgtggcatc gtgaaagtcg ctgcggttaa agcaccgggc ttcggcgatc 
     1261 gtcgtaaagc tatgctgcag gatatcgcaa ccctgactgg cggtaccgtg atctctgaag 
     1321 agatcggtat ggagctggaa aaagcaaccc tggaagacct gggtcaggct aaacgtgttg 
     1381 tgatcaacaa agacaccacc actatcatcg atggcgtggg tgaagaagct gcaatccagg 
     1441 gccgtgttgc tcagatccgt cagcagattg aagaagcaac ttctgactac gaccgtgaaa 
     1501 aactgcagga acgcgtagcg aaactggcag gcggcgttgc agttatcaaa gtgggtgctg 
     1561 ctaccgaagt tgaaatgaaa gagaaaaaag cacgcgttga agatgccctg cacgcgaccc 
     1621 gtgctgcggt agaagaaggc gtggttgctg gtggtggtgt tgcgctgatc cgcgtagcgt 
     1681 ctaaactggc tgacctgcgt ggtcagaacg aagaccagaa cgtgggtatc aaagttgcac 
     1741 tgcgtgcaat ggaagctccg ctgcgtcaga tcgtattgaa ctgcggcgaa gaaccgtctg 
     1801 ttgttgctaa caccgttaaa ggcggcgacg gcaactacgg ttacaacgca gcaaccgaag 
     1861 aatacggcaa catgatcgac atgggtatcc tggatccaac caaagtaact cgttctgctc 
     1921 tgcagtacgc agcttctgtg gctggcctga tgatcaccac cgaatgcatg gttaccgacc 
     1981 tgccgaaaaa cgatgcagct gacttaggcg ctgctggcgg tatgggcggc atgggtggca 
     2041 tgggcggcat gatgtaagct tgcggccgca taatgcttaa gtcgaacaga aagtaatcgt 
     2101 attgtacacg gccgcataat cgaaattaat acgactcact ataggggaat tgtgagcgga 
     2161 taacaattcc ccatcttagt atattagtta agtataagaa ggagatatac atatgcaagt 
     2221 ttcagttgaa accactcaag gccttggccg ccgtgtaacg attactgtcg ctgctgacag 
     2281 catcgagacc gctgttaaaa gcgagctggt caacgttgcg aaaaaagtac gtattgacgg 
     2341 cttccgcaaa ggcaaagtgc caatgaatat cgttgctcag cgttatggcg cgtctgtacg 
     2401 ccaggacgtt ctgggtgacc tgatgagccg taacttcatt gacgccatca ttaaagaaaa 
     2461 aatcaatccg gctggcgcac cgacttatgt tccgggcgaa tacaagctgg gtgaagactt 
     2521 cacttactct gtagagtttg aagtttatcc ggaagttgaa ctgcagggtc tggaagcgat 
     2581 cgaagttgaa aaaccgatcg ttgaagtgac cgacgctgac gttgacggca tgctggatac 
     2641 tctgcgtaaa cagcaggcga cctggaaaga aaaagacggc gctgttgaag cagaagaccg 
     2701 cgtaaccatc gacttcaccg gttctgtaga cggcgaagag ttcgaaggcg gtaaagcgtc 
     2761 tgatttcgta ctggcgatgg gccagggtcg tatgatcccg ggctttgaag acggtatcaa 
     2821 aggccacaaa gctggcgaag agttcaccat cgacgtgacc ttcccggaag aataccacgc 
     2881 agaaagcctg aaaggtaaag cagcgaaatt cgctatcaac ctgaagaaag ttgaagagcg 
     2941 tgaactgccg gaactgactg cagaattcat caaacgtttc ggcgttgaag atggttccgt 
     3001 agaaggtctg cgcgctgaag tgcgtaaaaa catggagcgc gagctgaaga gcgccatccg 
     3061 taaccgcgtt aagtctcagg cgatcgaagg tctggtaaaa gctaacgaca tcgacgtacc 
     3121 ggctgcgctg atcgacagcg aaatcgacgt tctgcgtcgc caggctgcac agcgtttcgg 
     3181 tggcaacgaa aaacaagctc tggaactgcc gcgcgaactg ttcgaagaac aggctaaacg 
     3241 ccgcgtagtt gttggcctgc tgctgggcga agttatccgc accaacgagc tgaaagctga 
     3301 cgaggagcgc gtgaaaggcc tgatcgaaga gatggcttct gcgtacgaag atccgaaaga 
     3361 agttatcgag ttctacagca aaaacaaaga actgatggac aacatgcgca atgttgctct 
     3421 ggaagaacag gctgttgaag ctgtactggc gaaagcgaaa gtgactgaaa aagaaaccac 
     3481 tttcaacgag ctgatgaacc agcaggcgta actcgagtct ggtaaagaaa ccgctgctgc 
     3541 gaaatttgaa cgccagcaca tggactcgtc tactagcgca gcttaattaa cctaggctgc 
     3601 tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg 
     3661 ttttttgctg aaacctcagg catttgagaa gcacacggtc acactgcttc cggtagtcaa 
     3721 taaaccggta aaccagcaat agacataagc ggctatttaa cgaccctgcc ctgaaccgac 
     3781 gaccgggtcg aatttgcttt cgaatttctg ccattcatcc gcttattatc acttattcag 
     3841 gcgtagcacc aggcgtttaa gggcaccaat aactgcctta aaaaaattac gccccgccct 
     3901 gccactcatc gcagtactgt tgtaattcat taagcattct gccgacatgg aagccatcac 
     3961 agacggcatg atgaacctga atcgccagcg gcatcagcac cttgtcgcct tgcgtataat 
     4021 atttgcccat agtgaaaacg ggggcgaaga agttgtccat attggccacg tttaaatcaa 
     4081 aactggtgaa actcacccag ggattggctg agacgaaaaa catattctca ataaaccctt 
     4141 tagggaaata ggccaggttt tcaccgtaac acgccacatc ttgcgaatat atgtgtagaa 
     4201 actgccggaa atcgtcgtgg tattcactcc agagcgatga aaacgtttca gtttgctcat 
     4261 ggaaaacggt gtaacaaggg tgaacactat cccatatcac cagctcaccg tctttcattg 
     4321 ccatacggaa ctccggatga gcattcatca ggcgggcaag aatgtgaata aaggccggat 
     4381 aaaacttgtg cttatttttc tttacggtct ttaaaaaggc cgtaatatcc agctgaacgg 
     4441 tctggttata ggtacattga gcaactgact gaaatgcctc aaaatgttct ttacgatgcc 
     4501 attgggatat atcaacggtg gtatatccag tgattttttt ctccatttta gcttccttag 
     4561 ctcctgaaaa tctcgataac tcaaaaaata cgcccggtag tgatcttatt tcattatggt 
     4621 gaaagttgga acctcttacg tgccgatcaa cgtctcattt tcgccaaaag ttggcccagg 
     4681 gcttcccggt atcaacaggg acaccaggat ttatttattc tgcgaagtga tcttccgtca 
     4741 caggtattta ttcggcgcaa agtgcgtcgg gtgatgctgc caacttactg atttagtgta 
     4801 tgatggtgtt tttgaggtgc tccagtggct tctgtttcta tcagctgtcc ctcctgttca 
     4861 gctactgacg gggtggtgcg taacggcaaa agcaccgccg gacatcagcg ctagcggagt 
     4921 gtatactggc ttactatgtt ggcactgatg agggtgtcag tgaagtgctt catgtggcag 
     4981 gagaaaaaag gctgcaccgg tgcgtcagca gaatatgtga tacaggatat attccgcttc 
     5041 ctcgctcact gactcgctac gctcggtcgt tcgactgcgg cgagcggaaa tggcttacga 
     5101 acggggcgga gatttcctgg aagatgccag gaagatactt aacagggaag tgagagggcc 
     5161 gcggcaaagc cgtttttcca taggctccgc ccccctgaca agcatcacga aatctgacgc 
     5221 tcaaatcagt ggtggcgaaa cccgacagga ctataaagat accaggcgtt tcccctggcg 
     5281 gctccctcgt gcgctctcct gttcctgcct ttcggtttac cggtgtcatt ccgctgttat 
     5341 ggccgcgttt gtctcattcc acgcctgaca ctcagttccg ggtaggcagt tcgctccaag 
     5401 ctggactgta tgcacgaacc ccccgttcag tccgaccgct gcgccttatc cggtaactat 
     5461 cgtcttgagt ccaacccgga aagacatgca aaagcaccac tggcagcagc cactggtaat 
     5521 tgatttagag gagttagtct tgaagtcatg cgccggttaa ggctaaactg aaaggacaag 
     5581 ttttggtgac tgcgctcctc caagccagtt acctcggttc aaagagttgg tagctcagag 
     5641 aaccttcgaa aaaccgccct gcaaggcggt tttttcgttt tcagagcaag agattacgcg 
     5701 cagaccaaaa cgatctcaag aagatcatct tattaatcag ataaaatatt tctagatttc 
     5761 agtgcaattt atctcttcaa atgtagcacc tgaagtcagc cccatacgat ataagttgta 
     5821 attctcatgt tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc 
     5881 aagggcatcg gtcgagatcc cggtgcctaa tgagtgagct aacttacatt aattgcgttg 
     5941 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc 
     6001 caacgcgcgg ggagaggcgg tttgcgtatt gggcgccagg gtggtttttc ttttcaccag 
     6061 tgagacgggc aacagctgat tgcccttcac cgcctggccc tgagagagtt gcagcaagcg 
     6121 gtccacgctg gtttgcccca gcaggcgaaa atcctgtttg atggtggtta acggcgggat 
     6181 ataacatgag ctgtcttcgg tatcgtcgta tcccactacc gagatgtccg caccaacgcg 
     6241 cagcccggac tcggtaatgg cgcgcattgc gcccagcgcc atctgatcgt tggcaaccag 
     6301 catcgcagtg ggaacgatgc cctcattcag catttgcatg gtttgttgaa aaccggacat 
     6361 ggcactccag tcgccttccc gttccgctat cggctgaatt tgattgcgag tgagatattt 
     6421 atgccagcca gccagacgca gacgcgccga gacagaactt aatgggcccg ctaacagcgc 
     6481 gatttgctgg tgacccaatg cgaccagatg ctccacgccc agtcgcgtac cgtcttcatg 
     6541 ggagaaaata atactgttga tgggtgtctg gtcagagaca tcaagaaata acgccggaac 
     6601 attagtgcag gcagcttcca cagcaatggc atcctggtca tccagcggat agttaatgat 
     6661 cagcccactg acgcgttgcg cgagaagatt gtgcaccgcc gctttacagg cttcgacgcc 
     6721 gcttcgttct accatcgaca ccaccacgct ggcacccagt tgatcggcgc gagatttaat 
     6781 cgccgcgaca atttgcgacg gcgcgtgcag ggccagactg gaggtggcaa cgccaatcag 
     6841 caacgactgt ttgcccgcca gttgttgtgc cacgcggttg ggaatgtaat tcagctccgc 
     6901 catcgccgct tccacttttt cccgcgtttt cgcagaaacg tggctggcct ggttcaccac 
     6961 gcgggaaacg gtctgataag agacaccggc atactctgcg acatcgtata acgttactgg 
     7021 tttcacattc accaccctga attgactctc ttccgggcgc tatcatgcca taccgcgaaa 
     7081 ggttttgcgc cattcgatgg tgtccgggat ctcgacgctc tcccttatgc gactcctgca 
     7141 ttaggaaatt aatacgactc actata 
//
