LOCUS       Exported               12703 bp ds-DNA    circular SYN 22-SEP-2016
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    pTK14 FUGW-U6-BsmBI-gRNA-UbC-eGFP-P2A-Bsr
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12703)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 22, 2016 from SnapGene 2.3.2
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..12703
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        225..423
                     /note="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             441..621
                     /note="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    668..793
                     /note="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1286..1519
                     /note="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    2046..2163
                     /note="cPPT/CTS"
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    2207..2240
                     /bound_moiety="Cre recombinase"
                     /note="loxP"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     misc_RNA        complement(2251..2326)
                     /note="gRNA scaffold"
                     /note="guide RNA scaffold for the CRISPR/Cas9 system"
     promoter        complement(4208..4456)
                     /note="U6 promoter"
                     /note="RNA polymerase III promoter for human U6 snRNA"
     promoter        4497..5699
                     /note="hUbC promoter"
     CDS             5728..6444
                     /codon_start=1
                     /product="enhanced GFP"
                     /note="EGFP"
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             6454..6510
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /note="P2A"
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             6511..6932
                     /codon_start=1
                     /note="Bsr"
                     /translation="KTFNISQQDLELVEVATEKITMLYEDNKHHVGAAIRTKTGEIISA
                     VHIEAYIGRVTVCAEAIAIGSAVSNGQKDFDTIVAVRHPYSDEVDRSIRVVSPCGMCRE
                     LISDYAPDCFVLIEMNGKLVKTTIEELIPLKYTRN"
     protein_bind    6943..6976
                     /bound_moiety="Cre recombinase"
                     /note="loxP"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     misc_feature    7001..7589
                     /note="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(7472..7483)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /note="Factor Xa site"
                     /translation="IEGR"
     primer_bind     complement(7592..7608)
                     /note="KS primer"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     LTR             8114..8294
                     /note="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     polyA_signal    8326..8550
                     /note="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8596..9024
                     /direction=RIGHT
                     /note="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        9038..9367
                     /note="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     rep_origin      9218..9353
                     /note="SV40 ori"
                     /note="SV40 origin of replication"
     promoter        9415..9462
                     /note="EM7 promoter"
                     /note="synthetic bacterial promoter "
     CDS             9481..9855
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /note="BleoR"
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    9985..10106
                     /note="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10155..10171)
                     /note="M13 rev"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    10179..10195
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(10203..10233)
                     /note="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    10248..10269
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding site"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(10557..11145)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(11316..12176)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(12177..12281)
                     /gene="bla"
                     /note="AmpR promoter"
     enhancer        12547..223
                     /note="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta
       61 aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt
      121 caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc
      181 tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca
      241 gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat
      301 tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa
      361 caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag
      421 cagcgcgttt tgcctgtact gggtctctct ggttagacca gatctgagcc tgggagctct
      481 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
      541 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
      601 cagtgtggaa aatctctagc agtggcgccc gaacagggac ttgaaagcga aagggaaacc
      661 agaggagctc tctcgacgca ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg
      721 gcggcgactg gtgagtacgc caaaaatttt gactagcgga ggctagaagg agagagatgg
      781 gtgcgagagc gtcagtatta agcgggggag aattagatcg cgatgggaaa aaattcggtt
      841 aaggccaggg ggaaagaaaa aatataaatt aaaacatata gtatgggcaa gcagggagct
      901 agaacgattc gcagttaatc ctggcctgtt agaaacatca gaaggctgta gacaaatact
      961 gggacagcta caaccatccc ttcagacagg atcagaagaa cttagatcat tatataatac
     1021 agtagcaacc ctctattgtg tgcatcaaag gatagagata aaagacacca aggaagcttt
     1081 agacaagata gaggaagagc aaaacaaaag taagaccacc gcacagcaag cggccgctga
     1141 tcttcagacc tggaggagga gatatgaggg acaattggag aagtgaatta tataaatata
     1201 aagtagtaaa aattgaacca ttaggagtag cacccaccaa ggcaaagaga agagtggtgc
     1261 agagagaaaa aagagcagtg ggaataggag ctttgttcct tgggttcttg ggagcagcag
     1321 gaagcactat gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa ttattgtctg
     1381 gtatagtgca gcagcagaac aatttgctga gggctattga ggcgcaacag catctgttgc
     1441 aactcacagt ctggggcatc aagcagctcc aggcaagaat cctggctgtg gaaagatacc
     1501 taaaggatca acagctcctg gggatttggg gttgctctgg aaaactcatt tgcaccactg
     1561 ctgtgccttg gaatgctagt tggagtaata aatctctgga acagatttgg aatcacacga
     1621 cctggatgga gtgggacaga gaaattaaca attacacaag cttaatacac tccttaattg
     1681 aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt attggaatta gataaatggg
     1741 caagtttgtg gaattggttt aacataacaa attggctgtg gtatataaaa ttattcataa
     1801 tgatagtagg aggcttggta ggtttaagaa tagtttttgc tgtactttct atagtgaata
     1861 gagttaggca gggatattca ccattatcgt ttcagaccca cctcccaacc ccgaggggac
     1921 ccgacaggcc cgaaggaata gaagaagaag gtggagagag agacagagac agatccattc
     1981 gattagtgaa cggatcggca ctgcgtgcgc caattctgca gacaaatggc agtattcatc
     2041 cacaatttta aaagaaaagg ggggattggg gggtacagtg caggggaaag aatagtagac
     2101 ataatagcaa cagacataca aactaaagaa ttacaaaaac aaattacaaa aattcaaaat
     2161 tttcgggttt attacaggga cagcagagat ccagtttggt taattaataa cttcgtatag
     2221 catacattat acgaagttat taaaaaaaaa gcaccgactc ggtgccactt tttcaagttg
     2281 ataacggact agccttattt taacttgcta tttctagctc taaaacagag acgtacaaaa
     2341 aagagcaaga agctaaaaaa gatttaaaaa ttatttttag cgcagttaat ggaacaggaa
     2401 ctaaatttac cccaaaaata ttacgtgaat caggatataa cgttattgag gttgaagagc
     2461 atgcatttga agatgaaaca tttaaaaatg ttgtaaatcc aaatccagaa tttgatcctg
     2521 catgaaaaat accgcttgaa tatggtatta aacatgatgc agatattatt attatgaatg
     2581 acccagatgc tgacagattt ggaatggcaa taaaacatga tggtcatttt gtaagattag
     2641 atggaaatca aacaggacca attttaattg attgaaaatt atcaaatcta aaacgcttaa
     2701 atagcattcc aaaaaatccg gctctatatt caagttttgt aacaagtgat ttgggtgata
     2761 gaatcgctca tgaaaaatat ggagttaata ttgtaaaaac tttaactgga tttaaatgaa
     2821 tgggtagaga aattgctaaa gaagaagata acggattaaa ttttgttttt gcttatgaag
     2881 aaagttatgg atatgtaatt gatgactcag ctagagataa agatggaata caagcttcta
     2941 tattaatagc agaggctgct tgattttata aaaaacaaaa taaaacatta gtagactatt
     3001 tagaagattt atttaaagaa atgggtgcat attacacttt cactttaaac ttgaatttta
     3061 aaccagaaga aaagaaatta aaaattgaac cattaatgaa atcattgaga gcaacaccct
     3121 taactcaaat tgctggactt aaagttgtta atgttgaaga ctacatcgat ggaatgtata
     3181 atatgccagg acaagactta ctaaaatttt atttagaaga taagtcatga tttgctgttc
     3241 gcccaagtgg aactgaacct aaactaaaaa tttattttat aggtgttggt gaatctgttc
     3301 aaaacgctaa agttaaagta gacgaaatta ttaaagaatt aaaattaaaa atgaatatat
     3361 aggagaaaaa atgaaactaa acaaatatat agatcacaca ttattaaaac aagatgctac
     3421 gaaagctgaa attaaacaat tatgtgatga agcaattgaa tttgattttg caacagtttg
     3481 tgttaattca tattgaacaa gctattgtaa agaattatta aaaggcacaa atgtaggaat
     3541 aacaaatgtt gtaggttttc ctctaggtgc atgcacaaca gctacaaaag cattcgaagt
     3601 ttctgaagca attaaagatg gtgcaacaga aattgatatg gtattaaata ttggtgcatt
     3661 aaaagacaaa aattatgaat tagttttaga agacatgaaa gctgtaaaaa aagcagctgg
     3721 atcacatgtt gttaaatgta ttatggaaaa ttgtttatta acaaaagaag aaatcatgaa
     3781 agcttgtgaa atagctgttg aagctggatt agaatttgtt aaaacatcaa caggattttc
     3841 aaaatcaggt gcaacatttg aagatgttaa actaatgaag tcagttgtta aagacaatgc
     3901 tttagttaaa gcagctggtg gagttagaac atttgaagat gctcaaaaaa tgattgaagc
     3961 aggagctgac cgcttaggaa caagtggtgg agtagctatt attaaaggtg aagaaaacaa
     4021 cgcgagttac taaaactagc gtttttttat tttgctcatt tttattaaaa gtttgcaaaa
     4081 aggaacataa aaattctaat tattgatact aaagttatta aaaagaagat tttggttgat
     4141 tttataaagg tcatagaata taatatttta gcatgtgtat tttgtgtgct catttacaac
     4201 cgtctccggt gtttcgtcct ttccacaaga tatataaagc caagaaatcg aaatactttc
     4261 aagttacggt aagcatatga tagtccattt taaaacataa ttttaaaact gcaaactacc
     4321 caagaaatta ttactttcta cgtcacgtat tttgtactaa tatctttgtg tttacagtca
     4381 aattaattcc aattatctct ctaacagcct tgtatcgtat atgcaaatat gaaggaatca
     4441 tgggaaatag gccctcttaa ttaattaacc cgtgtcggct ccagatctgg cctccgcgcc
     4501 gggttttggc gcctcccgcg ggcgcccccc tcctcacggc gagcgctgcc acgtcagacg
     4561 aagggcgcag gagcgttcct gatccttccg cccggacgct caggacagcg gcccgctgct
     4621 cataagactc ggccttagaa ccccagtatc agcagaagga cattttagga cgggacttgg
     4681 gtgactctag ggcactggtt ttctttccag agagcggaac aggcgaggaa aagtagtccc
     4741 ttctcggcga ttctgcggag ggatctccgt ggggcggtga acgccgatga ttatataagg
     4801 acgcgccggg tgtggcacag ctagttccgt cgcagccggg atttgggtcg cggttcttgt
     4861 ttgtggatcg ctgtgatcgt cacttggtga gttgcgggct gctgggctgg ccggggcttt
     4921 cgtggccgcc gggccgctcg gtgggacgga agcgtgtgga gagaccgcca agggctgtag
     4981 tctgggtccg cgagcaaggt tgccctgaac tgggggttgg ggggagcgca caaaatggcg
     5041 gctgttcccg agtcttgaat ggaagacgct tgtaaggcgg gctgtgaggt cgttgaaaca
     5101 aggtgggggg catggtgggc ggcaagaacc caaggtcttg aggccttcgc taatgcggga
     5161 aagctcttat tcgggtgaga tgggctgggg caccatctgg ggaccctgac gtgaagtttg
     5221 tcactgactg gagaactcgg gtttgtcgtc tggttgcggg ggcggcagtt atgcggtgcc
     5281 gttgggcagt gcacccgtac ctttgggagc gcgcgcctcg tcgtgtcgtg acgtcacccg
     5341 ttctgttggc ttataatgca gggtggggcc acctgccggt aggtgtgcgg taggcttttc
     5401 tccgtcgcag gacgcagggt tcgggcctag ggtaggctct cctgaatcga caggcgccgg
     5461 acctctggtg aggggaggga taagtgaggc gtcagtttct ttggtcggtt ttatgtacct
     5521 atcttcttaa gtagctgaag ctccggtttt gaactatgcg ctcggggttg gcgagtgtgt
     5581 tttgtgaagt tttttaggca ccttttgaaa tgtaatcatt tgggtcaata tgtaattttc
     5641 agtgttagac tagtaaattg tccgctaaat tctggccgtt tttggctttt ttgttagacg
     5701 aagcttgggc tgcaggtcga ctctagaatg gtgagcaagg gcgaggagct gttcaccggg
     5761 gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc
     5821 ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc
     5881 ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc
     5941 ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa
     6001 ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc
     6061 gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc
     6121 aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc
     6181 tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac
     6241 atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac
     6301 ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccgccct gagcaaagac
     6361 cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact
     6421 ctcggcatgg acgagctgta caagggatcc ggcgcaacaa acttctctct gctgaaacaa
     6481 gccggagatg tcgaagagaa tcctggaccg aagaccttca acatctctca gcaggatctg
     6541 gagctggtgg aggtcgccac tgagaagatc accatgctct atgaggacaa caagcaccat
     6601 gtcggggcgg ccatcaggac caagactggg gagatcatct ctgctgtcca cattgaggcc
     6661 tacattggca gggtcactgt ctgtgctgaa gccattgcca ttgggtctgc tgtgagcaac
     6721 gggcagaagg actttgacac cattgtggct gtcaggcacc cctactctga tgaggtggac
     6781 agatccatca gggtggtcag cccctgtggc atgtgcagag agctcatctc tgactatgct
     6841 cctgactgct ttgtgctcat tgagatgaat ggcaagctgg tcaaaaccac cattgaggaa
     6901 ctcatccccc tcaagtacac caggaactaa agttgataat agataacttc gtatagcata
     6961 cattatacga agttatgaat tcgatatcaa gcttatcgat aatcaacctc tggattacaa
     7021 aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata
     7081 cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc
     7141 cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg
     7201 tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac
     7261 ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat
     7321 cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt
     7381 ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat
     7441 tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc
     7501 ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag
     7561 tcggatctcc ctttgggccg cctccccgca tcgataccgt cgacctcgag acctagaaaa
     7621 acatggagca atcacaagta gcaatacagc agctaccaat gctgattgtg cctggctaga
     7681 agcacaagag gaggaggagg tgggttttcc agtcacacct caggtacctt taagaccaat
     7741 gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg
     7801 gctaattcac tcccaacgaa gacaagatat ccttgatctg tggatctacc acacacaagg
     7861 ctacttccct gattggcaga actacacacc agggccaggg atcagatatc cactgacctt
     7921 tggatggtgc tacaagctag taccagttga gcaagagaag gtagaagaag ccaatgaagg
     7981 agagaacacc cgcttgttac accctgtgag cctgcatggg atggatgacc cggagagaga
     8041 agtattagag tggaggtttg acagccgcct agcatttcat cacatggccc gagagctgca
     8101 tccggactgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
     8161 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
     8221 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
     8281 gaaaatctct agcagggccc gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt
     8341 gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc
     8401 ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt
     8461 ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca
     8521 ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc agctggggct
     8581 ctagggggta tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
     8641 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc
     8701 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt
     8761 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg
     8821 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca
     8881 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct
     8941 attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga
     9001 tttaacaaaa atttaacgcg aattaattct gtggaatgtg tgtcagttag ggtgtggaaa
     9061 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     9121 caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
     9181 ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag
     9241 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
     9301 cgcctctgcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt
     9361 ttgcaaaaag ctcccgggag cttgtatatc cattttcgga tctgatcagc acgtgttgac
     9421 aattaatcat cggcatagta tatcggcata gtataatacg acaaggtgag gaactaaacc
     9481 atggccaagt tgaccagtgc cgttccggtg ctcaccgcgc gcgacgtcgc cggagcggtc
     9541 gagttctgga ccgaccggct cgggttctcc cgggacttcg tggaggacga cttcgccggt
     9601 gtggtccggg acgacgtgac cctgttcatc agcgcggtcc aggaccaggt ggtgccggac
     9661 aacaccctgg cctgggtgtg ggtgcgcggc ctggacgagc tgtacgccga gtggtcggag
     9721 gtcgtgtcca cgaacttccg ggacgcctcc gggccggcca tgaccgagat cggcgagcag
     9781 ccgtgggggc gggagttcgc cctgcgcgac ccggccggca actgcgtgca cttcgtggcc
     9841 gaggagcagg actgacacgt gctacgagat ttcgattcca ccgccgcctt ctatgaaagg
     9901 ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc
     9961 atgctggagt tcttcgccca ccccaacttg tttattgcag cttataatgg ttacaaataa
    10021 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
    10081 ttgtccaaac tcatcaatgt atcttatcat gtctgtatac cgtcgacctc tagctagagc
    10141 ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
    10201 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
    10261 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
    10321 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
    10381 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
    10441 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
    10501 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
    10561 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
    10621 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
    10681 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
    10741 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
    10801 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
    10861 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
    10921 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
    10981 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
    11041 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
    11101 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
    11161 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
    11221 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
    11281 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
    11341 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
    11401 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
    11461 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
    11521 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
    11581 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
    11641 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
    11701 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
    11761 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
    11821 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
    11881 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
    11941 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
    12001 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
    12061 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
    12121 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
    12181 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
    12241 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
    12301 ccacctgacg tcgacggatc gggagatctc ccgatcccct atggtgcact ctcagtacaa
    12361 tctgctctga tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg
    12421 ctgagtagtg cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca
    12481 tgaagaatct gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata
    12541 cgcgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
    12601 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
    12661 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgt
//
