LOCUS       pUC57-Kan               4597 bp ss-DNA     circular SYN 22-Apr-2013
DEFINITION  -
ACCESSION   -
KEYWORDS    -
SOURCE      -
FEATURES             Location/Qualifiers
     primer_bind     379..394
                     /note="M13F"
     frag            407..2487
                     /note="407 to 2487 of pUC57-Kan-white_cf_sat_Bwx"
     exon            <407..487
                     /note="white exon 3"
     frag            407..2487
                     /note="5261 to 7341 of Untitled"
     frag            <407..>2472
                     /note="5226 to 7348 of white genomic DNA [Split]"
     exon            549..864
                     /note="white exon 4"
     exon            1068..1199
                     /note="white exon 5"
     exon            1270..1884
                     /note="white exon 6"
     misc_feature    1585..1587
                     /note="G589E"
     3'UTR           1885..2014
                     /note="white 3'-UTR"
     primer_bind     complement(2507..2523)
                     /note="M13-R"
     operon          complement(2529..2551)
                     /note="LacO"
     oriT            complement(2850..3523)
                     /note="pUC origin"
     CDS             complement(3675..4484)
                     /note="Kanamycin resistance gene"
BASE COUNT      1162 A      1127 C      1061 G      1247 T         0 OTHER
ORIGIN      ?
        1 TCGCGCGTTT CGGTGATGAC GGTGAAAACC TCTGACACAT GCAGCTCCCG GAGACGGTCA
       61 CAGCTTGTCT GTAAGCGGAT GCCGGGAGCA GACAAGCCCG TCAGGGCGCG TCAGCGGGTG
      121 TTGGCGGGTG TCGGGGCTGG CTTAACTATG CGGCATCAGA GCAGATTGTA CTGAGAGTGC
      181 ACCATATGCG GTGTGAAATA CCGCACAGAT GCGTAAGGAG AAAATACCGC ATCAGGCGCC
      241 ATTCGCCATT CAGGCTGCGC AACTGTTGGG AAGGGCGATC GGTGCGGGCC TCTTCGCTAT
      301 TACGCCAGCT GGCGAAAGGG GGATGTGCTG CAAGGCGATT AAGTTGGGTA ACGCCAGGGT
      361 TTTCCCAGTC ACGACGTTGT AAAACGACGG CCAGTGAATT CGAGCTCTTT GACAAGATCC
      421 TTCTGATGGC CGAGGGCAGG GTAGCTTTCT TGGGCACTCC CAGCGAAGCC GTCGACTTCT
      481 TTTCCTAGTG AGTTCGATGT GTTTATTAAG GGTATCTAGT ATTACATAAC ATCTCAACTC
      541 CTATCCAGCG TGGGTGCCCA GTGTCCTACC AACTACAATC CGGCGGACTT TTACGTACAG
      601 GTGTTGGCCG TTGTGCCCGG ACGGGAGATC GAGTCCCGTG ATCGGATCGC CAAGATATGC
      661 GACAATTTTG CCATTAGCAA AGTAGCCCGG GATATGGAGC AGTTGTTGGC CACCAAAAAT
      721 CTGGAGAAGC CACTGGAGCA GCCGGAGAAT GGGTACACCT ACAAGGCCAC CTGGTTCATG
      781 CAGTTCCGGG CGGTCCTGTG GCGATCCTGG CTGTCGGTGC TCAAGGAACC ACTCCTCGTA
      841 AAAGTGCGAC TTATTCAGAC AACGGTGAGT GGTTCCAGTG GAAACAAATG ATATAACGCT
      901 TACAATTCTT GGAAACAAAT TCGCTAGATT TTAGATAGAA TTGCCTGATT CCACACCCTT
      961 CTTAGTTTTT TTCAATGAGA TGTATAGTTT ATAGTTTTGC AGAAGATAAA TAAATTTCAT
     1021 TTAACTCGCG AATATTAATG AGATGCGAGT AACATTTTAA TTTGCAGATG GTTGCCATCT
     1081 TGATTGGCCT CATCTTTTTG GGCCAACAAC TCACGCAAGT GGGTGTGATG AATATCAACG
     1141 GAGCCATCTT CCTCTTCCTG ACCAACATGA CCTTTCAAAA CGTCTTTGCC ACGATAAATG
     1201 TAAGTCATGT TTAGAATACA TTTGCATTTC AATAATTTAC TAACTTTCTA ATGAATCGAT
     1261 TCGATTTAGG TGTTCACCTC AGAGCTGCCA GTTTTTATGA GGGAGGCCCG AAGTCGACTT
     1321 TATCGCTGTG ACACATACTT TCTGGGCAAA ACGATTGCCG AATTGCCGCT TTTTCTCACA
     1381 GTGCCACTGG TCTTCACGGC GATTGCCTAT CCGATGATCG GACTGCGGGC CGGAGTGCTG
     1441 CACTTCTTCA ACTGCCTGGC GCTGGTCACT CTGGTGGCCA ATGTGTCAAC GTCCTTCGGA
     1501 TATCTAATAT CCTGCGCCAG CTCCTCGACC TCGATGGCGC TGTCTGTGGG TCCGCCGGTT
     1561 ATCATACCAT TCCTGCTCTT TGGCGAATTC TTCTTGAACT CGGGCTCGGT GCCAGTTTAC
     1621 CTCAAATGGT TGTCGTACCT CTCATGGTTC CGTTACGCCA ACGAGGGTCT GCTGATTAAC
     1681 CAATGGGCGG ACGTGGAGCC GGGCGAAATT AGCTGCACAT CGTCGAACAC CACGTGCCCC
     1741 AGTTCGGGCA AGGTCATCCT GGAGACGCTT AACTTCTCGG CCGCCGATCT GCCGCTGGAC
     1801 TACGTGGGTC TGGCCATTCT CATCGTGAGC TTCCGGGTGC TCGCATATCT GGCTCTAAGA
     1861 CTTCGGGCCC GACGCAAGGA GTAGCCGACA TATATCCGAA ATAACTGCTT GTTTTTTTTT
     1921 TTACCATTAT TACCATCGTG TTTACTGTTT ATTGCCCCCT CAAAAAGCTA ATGTAATTAT
     1981 ATTTGTGCCA ATAAAAACAA GATATGACCT ATAGAATACA AGTATTTCCC CTTCGAACAT
     2041 CCCCACAAGT AGACTTTGGA TTTGTCTTCT AACCAAAAGA CTTACACACC TGCATACCTT
     2101 ACATCAAAAA CTCGTTTATC GCTACATAAA ACACCGGGAT ATATTTTTTA TATACAAACT
     2161 TTTCAAATCG CGCGCCCTCT TCATAATTCA CCTCCACCAC ACCACGTTTC GTAGTTGCTC
     2221 TTTCGCTGTC TCCCACCCGC TCTCCGCAAC ACATTCACCT TTTGTTCGAC GACCTTGGAG
     2281 CGACTGTCGT TAGTTCCGCG CGATTCGGTT CGCTCAAATG GTTCCGAGTG GTTCATTTCG
     2341 TCTCAATAGA AATTAGTAAT AAATATTTGT ATGTACAATT TATTTGCTCC AATATATTTG
     2401 TATATATTTC CCTCACAGCT ATATTTATTC TAATTTAATA TTATGACTTT TTAAGGTAAT
     2461 TTTTTGTGAC CTGTTCGGAG TGGCATGCAA GCTTGGCGTA ATCATGGTCA TAGCTGTTTC
     2521 CTGTGTGAAA TTGTTATCCG CTCACAATTC CACACAACAT ACGAGCCGGA AGCATAAAGT
     2581 GTAAAGCCTG GGGTGCCTAA TGAGTGAGCT AACTCACATT AATTGCGTTG CGCTCACTGC
     2641 CCGCTTTCCA GTCGGGAAAC CTGTCGTGCC AGCTGCATTA ATGAATCGGC CAACGCGCGG
     2701 GGAGAGGCGG TTTGCGTATT GGGCGCTCTT CCGCTTCCTC GCTCACTGAC TCGCTGCGCT
     2761 CGGTCGTTCG GCTGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA
     2821 CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA
     2881 ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC
     2941 ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG
     3001 CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT
     3061 ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT
     3121 ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC
     3181 AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG
     3241 ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG
     3301 GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG
     3361 GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG
     3421 GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA
     3481 GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA
     3541 ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA
     3601 TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAAGCCCAAT CTGAATAATG TTACAACCAA
     3661 TTAACCAATT CTGATTAGAA AAACTCATCG AGCATCAAAT GAAACTGCAA TTTATTCATA
     3721 TCAGGATTAT CAATACCATA TTTTTGAAAA AGCCGTTTCT GTAATGAAGG AGAAAACTCA
     3781 CCGAGGCAGT TCCATAGGAT GGCAAGATCC TGGTATCGGT CTGCGATTCC GACTCGTCCA
     3841 ACATCAATAC AACCTATTAA TTTCCCCTCG TCAAAAATAA GGTTATCAAG TGAGAAATCA
     3901 CCATGAGTGA CGACTGAATC CGGTGAGAAT GGCAAAAGTT TATGCATTTC TTTCCAGACT
     3961 TGTTCAACAG GCCAGCCATT ACGCTCGTCA TCAAAATCAC TCGCATCAAC CAAACCGTTA
     4021 TTCATTCGTG ATTGCGCCTG AGCGAGACGA AATACGCGAT CGCTGTTAAA AGGACAATTA
     4081 CAAACAGGAA TCGAATGCAA CCGGCGCAGG AACACTGCCA GCGCATCAAC AATATTTTCA
     4141 CCTGAATCAG GATATTCTTC TAATACCTGG AATGCTGTTT TTCCGGGGAT CGCAGTGGTG
     4201 AGTAACCATG CATCATCAGG AGTACGGATA AAATGCTTGA TGGTCGGAAG AGGCATAAAT
     4261 TCCGTCAGCC AGTTTAGTCT GACCATCTCA TCTGTAACAT CATTGGCAAC GCTACCTTTG
     4321 CCATGTTTCA GAAACAACTC TGGCGCATCG GGCTTCCCAT ACAAGCGATA GATTGTCGCA
     4381 CCTGATTGCC CGACATTATC GCGAGCCCAT TTATACCCAT ATAAATCAGC ATCCATGTTG
     4441 GAATTTAATC GCGGCCTCGA CGTTTCCCGT TGAATATGGC TCATAACACC CCTTGTATTA
     4501 CTGTTTATGT AAGCAGACAG TTTTATTGTT CATGATGATA TATTTTTATC TTGTGCAATG
     4561 TAACATCAGA GATTTTGAGA CACGGGCCAG AGCTGCA
//
