LOCUS pHD066_elastaseP_t 9635 bp ds-DNA linear 15-SEP-2010 DEFINITION . ACCESSION VERSION SOURCE . ORGANISM . COMMENT COMMENT ApEinfo:methylated:1 FEATURES Location/Qualifiers primer_bind complement(6814..6833) /label=T3 /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green primer_bind complement(6867..6884) /label=M13-fwd /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green primer_bind 42..62 /label=T7 /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green rep_origin 8566..9248 /label=ColE1 origin /ApEinfo_fwdcolor=gray50 /ApEinfo_revcolor=gray50 rep_origin 7042..7348 /label=F1 ori /ApEinfo_fwdcolor=gray50 /ApEinfo_revcolor=gray50 CDS 6955..7023 /label=LacZ alpha /ApEinfo_fwdcolor=#6495ed /ApEinfo_revcolor=#6495ed misc_binding 9588..9610 /label=LacO /ApEinfo_fwdcolor=#6495ed /ApEinfo_revcolor=#6495ed misc_recomb 5219..5252 /label=FRT /ApEinfo_fwdcolor=orchid /ApEinfo_revcolor=pink CDS 7809..8468 /label=AmpR /ApEinfo_fwdcolor=yellow /ApEinfo_revcolor=yellow polyA_signal complement(4992..5183) /label=SV40 late polyA /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink misc_feature 18..35 /label=I-SceI /ApEinfo_fwdcolor=#ffff00 /ApEinfo_revcolor=#ffff00 misc_feature 6841..6858 /label=I-SceI(1) /ApEinfo_label=I-SceI /ApEinfo_fwdcolor=#ffff00 /ApEinfo_revcolor=#ffff00 misc_feature 3004..3711 /label=mCherry /ApEinfo_fwdcolor=#0000ff /ApEinfo_revcolor=#0000ff promoter complement(6229..6791) /label=Crystalin Promoter /ApEinfo_fwdcolor=#ff8000 /ApEinfo_revcolor=#ff8000 CDS complement(5507..6226) /label=Venus /ApEinfo_fwdcolor=#0000ff /ApEinfo_revcolor=#0000ff polyA_site complement(5276..5501) /label=bGH pA /ApEinfo_fwdcolor=#ff00ff /ApEinfo_revcolor=#ff00ff misc_feature 76..2946 /label=elastase promoter /ApEinfo_fwdcolor=#ff8000 /ApEinfo_revcolor=#ff8000 promoter 76..2946 /label=Elastase Promoter /ApEinfo_fwdcolor=#ff8000 /ApEinfo_revcolor=#ff8000 ORIGIN 1 GATTACGCCA AGCGCGTTAG GGATAACAGG GTAATGCGCG CGTAATACGA CTCACTATAG 61 GGCGAATTGg agctctcgac ttcgatcctc cgcaggtgtg gtaaaatgag cttccactct 121 gatactgcag agagacctaa taatgggaca cacaatgaat atatttaata ataataattt 181 cttacattta tatagtgctt ttctagacgc tcaaatcgct ttacacaatg gaggggatct 241 cctcatccac caccagtttt ttgaaagaca tccaggtatt ttaaacgaac acccagtcca 301 catcaatata ctgggatttg acaatcgatt tgtgttggga caacatgaat gaattaagtt 361 aacttaacac tttaaacgaa tttgtttgaa cataaaaaat taagttgtcc caaggaaatc 421 tgttgtattg ttgtagctaa ttttaaatag ttagtttgaa ggagcaaaaa taaaagtttt 481 tattgagttt gagaaatatt gtcttgtttt cagaaataaa gtcacaatta agtgagtttt 541 tccctaaaat aagttaaatc ttatataaca agcttattgt cacagaaaaa aaacatcaag 601 cagtacttta tcgaattaaa catgtatctt tcatttcaga atattatttg tttaagtaat 661 ttaacagata attatttgtt acttgttatt tgtcaagtaa tttaagttca aaacacagtt 721 aaatatgtgt ttttttatta ggagctcaac taagaaaaat ttgttttgac caattttagg 781 ggttactcac acaggttagt gcatgcgtat taaacaaaaa ctcagtcata tcttaaaaaa 841 tatttggcaa atgatttctt tttcattggc tcaagtaatc aaatctagct gaggatttaa 901 attaattgac tttcttcaac aagacaaata tgctttgttc caggttatat aaaattgctc 961 tcatggagat agaaaatatt gtttaaggca aattatttat ttttaattgg aacaagtgat 1021 aaaaatgtag atgtttatat taccccattt ttttaaaaac tggatgaatg aaagcttttt 1081 ccagtgtact taccccactg acagactgtt ttgcttgaat tatgttaatt acttgggtaa 1141 aacatgtatt ccaaatctga taatatacaa taacaagctg tagttcatat ttaagccatc 1201 tgaaatgtta attactgtga gcatttagtc ttttacggaa aacagataga aatcacgtca 1261 tgaaaacaaa taacattagg ttacaagcag tacaaatttg catggattaa ttaccttcta 1321 gtcaattttg agttgagata ttttccatga gataacaaaa cccttaaact gatcagcagt 1381 gtggtgacgg aacaacagca gcacaataag aacctcatga tctctccggt ctcctttatc 1441 tttgacagat ttcaggaaca ccctgttatc tagatgagat taaagcatct ccatgtggga 1501 tgatgagttt tctgcaggcc gttaagggtg aatattcata cattatcctt tgaatgaggt 1561 catctgatac aatagttcct gtgaggatga ataatgagtt ttaggattaa aagccataag 1621 taaacagtca cacttttaaa ttcccagatt gcaaaggctt tgctttgagc aagccataaa 1681 gtttctgtaa ggtgtctttg tttagatcaa gcaattttac ataaacaaat ttcaaatcca 1741 cacactaaac atctttttag cataaaaaca ttttttaaaa gggcgacacg gtggcacagt 1801 gggtagcact gtcgccttac aacaagaagg tcactggtgt gtgcccaggc tgaatcagtt 1861 ggcatttctg tgtggagttt gcatgttctc cccatgtgcg taggtttcct ccgggtgctc 1921 tggtttcccc cacacttcaa aaacatgcgc tataggtgaa ttgaataagc taaaatagcc 1981 atagtgtatg tgcgtgaatg caagagtgta tggtgtttcc ctgtgctggg ttgcagctgg 2041 aagttatgcg taaaacatat gctggataag ttggcggtta attctgctgt ggcaacccct 2101 gattaataat gggactaagc tgaaaaaaat gaataaatga attaatattt ttacaagaat 2161 gtacacacat tttatggttc aatggtgaga tatcataaaa cacaagctgg gcaatacacg 2221 tgttttatca taggatacta tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg tgtgttaaat 2281 gtatgaaaca cataaatgta aatgcacata ctaatatgtg ctcattaagc attacaattt 2341 tgcttaattt tcagagccaa aaaaagcaac acagacgtat gtcaatcctg ctgctaacct 2401 cttttctctg agaactcacc atctgctgac ctccttcata caagaccaca ggtacattca 2461 aatgtaagat tattcacaga gagaaaaata attattcaca aaccccatat gctatgggat 2521 atatagtact aaatttgggt ctgtgagagc tgtaatattt tcatttgtat tcaaaagtga 2581 gagtgtcttt agtgtattaa tagattgttg aatcaaataa agctgatgat gtgtgacaac 2641 tgcctgaggt aaatattaaa aataaaaacc cattttcttc caatggttga tctctttctt 2701 atgggaattc taaatagtct agaactgtat gcttatgcta atatataaat caagagaact 2761 agtcaagtca gaggtttgtg cttgaaggcc atgtgaaagg tttcaaagaa agcagctgtt 2821 caaaggctga taattgtttt tccagctata acaggtggaa tactcagcaa tattgtttag 2881 aatattgtgt ggtcaatgtg acatcacaga tagagagggt ttaaaaagca gttgatgcca 2941 gtgttgcgga tctaattcga tatcaagctt atcgatatgg ctccaaagaa gaagcgtaag 3001 gtaATGGTGA GCAAGGGCGA GGAGGATAAC ATGGCCATCA TCAAGGAGTT CATGCGCTTC 3061 AAGGTGCACA TGGAGGGCTC CGTGAACGGC CACGAGTTCG AGATCGAGGG CGAGGGCGAG 3121 GGCCGCCCCT ACGAGGGCAC CCAGACCGCC AAGCTGAAGG TGACCAAGGG TGGCCCCCTG 3181 CCCTTCGCCT GGGACATCCT GTCCCCTCAG TTCATGTACG GCTCCAAGGC CTACGTGAAG 3241 CACCCCGCCG ACATCCCCGA CTACTTGAAG CTGTCCTTCC CCGAGGGCTT CAAGTGGGAG 3301 CGCGTGATGA ACTTCGAGGA CGGCGGCGTG GTGACCGTGA CCCAGGACTC CTCCCTGCAG 3361 GACGGCGAGT TCATCTACAA GGTGAAGCTG CGCGGCACCA ACTTCCCCTC CGACGGCCCC 3421 GTAATGCAGA AGAAGACCAT GGGCTGGGAG GCCTCCTCCG AGCGGATGTA CCCCGAGGAC 3481 GGCGCCCTGA AGGGCGAGAT CAAGCAGAGG CTGAAGCTGA AGGACGGCGG CCACTACGAC 3541 GCTGAGGTCA AGACCACCTA CAAGGCCAAG AAGCCCGTGC AGCTGCCCGG CGCCTACAAC 3601 GTCAACATCA AGTTGGACAT CACCTCCCAC AACGAGGACT ACACCATCGT GGAACAGTAC 3661 GAACGCGCCG AGGGCCGCCA CTCCACCGGC GGCATGGACG AGCTGTACAA Ggcagctagc 3721 gcaATGTCCA ATTTACTGAC CGTACACCAA AATTTGCCTG CATTACCGGT CGATGCAACG 3781 AGTGATGAGG TTCGCAAGAA CCTGATGGAC ATGTTCAGGG ATCGCCAGGC GTTTTCTGAG 3841 CATACCTGGA AAATGCTTCT GTCCGTTTGC CGGTCGTGGG CGGCATGGTG CAAGTTGAAT 3901 AACCGGAAAT GGTTTCCCGC AGAACCTGAA GATGTTCGCG ATTATCTTCT ATATCTTCAG 3961 GCGCGCGGTC TGGCAGTAAA AACTATCCAG CAACATTTGG GCCAGCTAAA CATGCTTCAT 4021 CGTCGGTCCG GGCTGCCACG ACCAAGTGAC AGCAATGCTG TTTCACTGGT TATGCGGCGG 4081 ATCCGAAAAG AAAACGTTGA TGCCGGTGAA CGTGCAAAAC AGGCTCTAGC GTTCGAACGC 4141 ACTGATTTCG ACCAGGTTCG TTCACTCATG GAAAATAGCG ATCGCTGCCA GGATATACGT 4201 AATCTGGCAT TTCTGGGGAT TGCTTATAAC ACCCTGTTAC GTATAGCCGA AATTGCCAGG 4261 ATCAGGGTTA AAGATATCTC ACGTACTGAC GGTGGGAGAA TGTTAATCCA TATTGGCAGA 4321 ACGAAAACGC TGGTTAGCAC CGCAGGTGTA GAGAAGGCAC TTAGCCTGGG GGTAACTAAA 4381 CTGGtCNAgC GAtGGATTTc CGTCTCTGGT GTAGCTGATG ATCCGAATAA CTACCTGTTT 4441 TGCCGGGTCA GAAAAAATGG TGTTGCCGCG CCATCTGCCA CCAGCCAGCT ATCAACTCGC 4501 GCCCTGGAAG GGATTTTTGA AGCAACTCAT CGATTGATTT ACGGCGCTAA GGATGACTCT 4561 GGTCAGAGAT ACCTGGCCTG GTCTGGACAC AGTGCCCGTG TCGGAGCCGC GCGAGATATG 4621 GCCCGCGCTG GAGTTTCAAT ACCGGAGATC ATGCAAGCTG GTGGCTGGAC CAATGTAAAT 4681 ATTGTCATGA ACTATATCCG TAACCTGGAT AGTGAAACAG GGGCAATGGT GCGCCTGCTG 4741 GAAGATGGCG ATTAGGCggc cgccgaaatg accgaccaag cgacgcccaa cctgccatca 4801 cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg 4861 gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccacccc 4921 ccggatctaa gctctagata agtaatgatc ataatcagcc atatcacatc tgtagaggtt 4981 ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca 5041 attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc 5101 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc 5161 atcaatgtat cttatcatgt ctggatccgg gggtaccgcg tcgagaagtt cctattccga 5221 agttcctatt ctctagaaag tataggaact tcgggccCGC GGTGGCGGCG ATCTCTCCCC 5281 AGCATGCCTG CTATTGTCTT CCCAATCCTC CCCCTTGCTG TCCTGCCCCA CCCCACCCCC 5341 CAGAATAGAA TGACACCTAC TCAGACAATG CGATGCAATT TCCTCATTTT ATTAGGAAAG 5401 GACAGTGGGA GTGGCACCTT CCAGGGTCAA GGAAGGCACG GGGGAGGGGC AAACAACAGA 5461 TGGCTGGCAA CTAGAAGGCA CAGTCGAGGC TGATCAGCGG GCCGCTTTAC TTGTACAGCT 5521 CGTCCATGCC GAGAGTGATC CCGGCGGCGG TCACGAACTC CAGCAGGACC ATGTGATCGC 5581 GCTTCTCGTT GGGGTCTTTG CTCAGGGCGG ACTGGTAGCT CAGGTAGTGG TTGTCGGGCA 5641 GCAGCACGGG GCCGTCGCCG ATGGGGGTGT TCTGCTGGTA GTGGTCGGCG AGCTGCACGC 5701 CGCCGTCCTC GATGTTGTGG CGGATCTTGA AGTTGGCCTT GATGCCGTTC TTCTGCTTGT 5761 CGGCGGTGAT ATAGACGTTG TGGCTGTTGT AGTTGTACTC CAGCTTGTGC CCCAGGATGT 5821 TGCCGTCCTC CTTGAAGTCG ATGCCCTTCA GCTCGATGCG GTTCACCAGG GTGTCGCCCT 5881 CGAACTTCAC CTCGGCGCGG GTCTTGTAGT TGCCGTCGTC CTTGAAGAAG ATGGTGCGCT 5941 CCTGGACGTA GCCTTCGGGC ATGGCGGACT TGAAGAAGTC GTGCTGCTTC ATGTGGTCGG 6001 GGTAGCGGGC GAAGCACTGC AGGCCGTAGC CCAGGGTGGT CACGAGGGTG GGCCAGGGCA 6061 CGGGCAGCTT GCCGGTGGTG CAGATCAGCT TCAGGGTCAG CTTGCCGTAG GTGGCATCGC 6121 CCTCGCCCTC GCCGGACACG CTGAACTTGT GGCCGTTTAC GTCGCCGTCC AGCTCGACCA 6181 GGATGGGCAC CACCCCGGTG AACAGCTCCT CGCCCTTGCT CACCATGTTG TCAGACCTGG 6241 TAACTCCTTA CTGTAACTTA TTGCACCTGA TGAACAAAAG ATGTGAACTG AACGCAAGTA 6301 GGAGGCTAAT AGAGGCTAGT ACAGTACAAA GATCCCCAAA TGATGTGTGC CGGCCTTAGT 6361 GGAGGCAGAC CCCTGGTTAT ATACCTGTGC TGCAGAAAGA GGGGCTTACA AATGGATCTC 6421 TCTGGAATGG CATTATCTCA TGGTGGGAAC AGTGTTGTCA GCAGAATCAG CGGGGTGGAC 6481 TCTGACCTGA GAATGACAGT CTAGCACTGA ATGGCTCAGA CAATACTCTT GTGGCCAACT 6541 ATCACCGGAA AATAAGTGCC TGCCAAAACA AACCAGACCA GATGAAGCTA CATGGCTGTA 6601 GAACGTCAGT ATTTAATGTG TTGTGGCCAC TTGGTTCACC AGATTTAAGA GCTAATTTTT 6661 TTAAATATCA CAGATAATCA ATCGCTTTTT TTTTTTCTTG ACTGAAAAGC TTAGACTGGA 6721 AAATTCTTTG AAAAAGTTTG CACTACAGAA AATAAATACA TAACATCCTG CACTGAATGC 6781 ACACTATTAA TgggccCGGT ACCCAGCTTT TGTTCCCTTT AGTGAGGGTT AATTGCGCGC 6841 TAGGGATAAC AGGGTAATAC GCGCTCactg gccgtcgttt tacaacgtcg tgactgggaa 6901 aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt 6961 aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa 7021 tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg 7081 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc 7141 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga 7201 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt 7261 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat 7321 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat 7381 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa 7441 tttaacgcga attttaacaa aatattaacg cttacaattt aggtggcact tttcggggaa 7501 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca 7561 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc 7621 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc 7681 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt 7741 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt 7801 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg 7861 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact 7921 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg 7981 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga 8041 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg 8101 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa 8161 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac 8221 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc 8281 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca 8341 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga 8401 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta 8461 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc 8521 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc 8581 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt 8641 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac 8701 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct 8761 tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact 8821 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg 8881 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata 8941 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga 9001 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag 9061 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg 9121 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac 9181 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca 9241 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg 9301 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc 9361 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa 9421 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt 9481 ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt 9541 aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg 9601 gataacaatt tcacacagga aacagctatg accat //