LOCUS pSLQ1852_5_pHR_U 7656 bp ds-DNA circular 30-SEP-2016 DEFINITION . ACCESSION VERSION SOURCE . ORGANISM . COMMENT U6_sgRNA_template from 1 to 8308 COMMENT pMig-w from 1 to 6471 COMMENT COMMENT ApEinfo:methylated:1 FEATURES Location/Qualifiers misc_feature join(2919..2919,2934..2936,2940..2944) /label=mU6 promoter (modified) /ApEinfo_fwdcolor="#fff431" /ApEinfo_revcolor="#fff431" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" CDS complement(6666..7325) /label=AmpR /ApEinfo_fwdcolor="#ffff00" /ApEinfo_revcolor="#ffff00" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" rep_origin complement(5886..6568) /label=ColE1 origin /ApEinfo_fwdcolor="gray50" /ApEinfo_revcolor="gray50" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_recomb 2825..2858 /label=LoxP recombination site /ApEinfo_fwdcolor="steel blue" /ApEinfo_revcolor="orange" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_recomb 4456..4489 /label=LoxP recombination site(1) /ApEinfo_label="LoxP recombination site" /ApEinfo_fwdcolor="steel blue" /ApEinfo_revcolor="orange" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature complement(7565..7593) /label=AmpR promoter /ApEinfo_fwdcolor="#ffff00" /ApEinfo_revcolor="#ffff00" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 844..1024 /label=HIV-1_5_LTR /ApEinfo_fwdcolor="cyan" /ApEinfo_revcolor="cyan" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 3717..4436 /label=EGFP /ApEinfo_fwdcolor="#00ff00" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 5662..5842 /label=HIV-1_5_LTR(1) /ApEinfo_label="HIV-1_5_LTR" /ApEinfo_fwdcolor="cyan" /ApEinfo_revcolor="cyan" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 1695..1928 /label=RRE /ApEinfo_fwdcolor="#9cffe3" /ApEinfo_revcolor="#9cffe3" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 2459..2473 /label=cPPT /ApEinfo_fwdcolor="cyan" /ApEinfo_revcolor="cyan" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 5325..5339 /label=cPPT(1) /ApEinfo_label="cPPT" /ApEinfo_fwdcolor="cyan" /ApEinfo_revcolor="cyan" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 4535..5135 /label=wPRE /ApEinfo_fwdcolor="#0060ff" /ApEinfo_revcolor="#0060ff" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature complement(5889..6508) /label=pBR322_origin /ApEinfo_fwdcolor="#95928e" /ApEinfo_revcolor="#95928e" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 3116..3698 /label=CMV promoter IE /ApEinfo_fwdcolor="#ffed31" /ApEinfo_revcolor="#ffed31" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 263..826 /label=pU6 (pMJ177) 5' CMV promoter IE /ApEinfo_fwdcolor="#fffc15" /ApEinfo_revcolor="#fffc15" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 3116..3679 /label=pU6 (pMJ177) 5' CMV promoter IE(1) /ApEinfo_label="pU6 (pMJ177) 5' CMV promoter IE" /ApEinfo_fwdcolor="#fffc15" /ApEinfo_revcolor="#fffc15" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature complement(1601..1607) /label=TATA box /ApEinfo_fwdcolor="#ff9afa" /ApEinfo_revcolor="#ff9afa" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature complement(766..775) /label=GC Box /ApEinfo_fwdcolor="#888eff" /ApEinfo_revcolor="#888eff" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature complement(3619..3628) /label=GC Box(1) /ApEinfo_label="GC Box" /ApEinfo_fwdcolor="#888eff" /ApEinfo_revcolor="#888eff" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature complement(5913..5922) /label=GC Box(2) /ApEinfo_label="GC Box" /ApEinfo_fwdcolor="#888eff" /ApEinfo_revcolor="#888eff" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 2631..2944 /label=mU6 promoter (modified)(1) /ApEinfo_label="mU6 promoter (modified)" /ApEinfo_fwdcolor="#fff431" /ApEinfo_revcolor="#fff431" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 2966..3058 /label=modified stem loop /ApEinfo_fwdcolor="cyan" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 2945..2965 /label=sp sgCD95-3 /ApEinfo_fwdcolor="#ff80ff" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" ORIGIN 1 gcttaagcgg tcgacggatc gggagatctc ccgatcccct atggtgcact ctcagtacaa 61 tctgctctga tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg 121 ctgagtagtg cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca 181 tgaagaatct gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata 241 cgcgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc 301 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac 361 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa 421 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag 481 tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc 541 ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct 601 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg 661 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt 721 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga 781 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagcgcg ttttgcctgt 841 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac 901 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg 961 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct 1021 agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac 1081 gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta 1141 cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta 1201 ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga 1261 aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta 1321 atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat 1381 cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca accctctatt 1441 gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag 1501 agcaaaacaa aagtaagacc accgcacagc aagcggccgg ccgcgctgat cttcagacct 1561 ggaggaggag atatgaggga caattggaga agtgaattat ataaatataa agtagtaaaa 1621 attgaaccat taggagtagc acccaccaag gcaaagagaa gagtggtgca gagagaaaaa 1681 agagcagtgg gaataggagc tttgttcctt gggttcttgg gagcagcagg aagcactatg 1741 ggcgcagcgt caatgacgct gacggtacag gccagacaat tattgtctgg tatagtgcag 1801 cagcagaaca atttgctgag ggctattgag gcgcaacagc atctgttgca actcacagtc 1861 tggggcatca agcagctcca ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa 1921 cagctcctgg ggatttgggg ttgctctgga aaactcattt gcaccactgc tgtgccttgg 1981 aatgctagtt ggagtaataa atctctggaa cagatttgga atcacacgac ctggatggag 2041 tgggacagag aaattaacaa ttacacaagc ttaatacact ccttaattga agaatcgcaa 2101 aaccagcaag aaaagaatga acaagaatta ttggaattag ataaatgggc aagtttgtgg 2161 aattggttta acataacaaa ttggctgtgg tatataaaat tattcataat gatagtagga 2221 ggcttggtag gtttaagaat agtttttgct gtactttcta tagtgaatag agttaggcag 2281 ggatattcac cattatcgtt tcagacccac ctcccaaccc cgaggggacc cgacaggccc 2341 gaaggaatag aagaagaagg tggagagaga gacagagaca gatccattcg attagtgaac 2401 ggatcggcac tgcgtgcgcc aattctgcag acaaatggca gtattcatcc acaattttaa 2461 aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac 2521 agacatacaa actaaagaat tacaaaaaca aattacaaaa attcaaaatt ttcgggttta 2581 ttacagggac agcagagatc cagtttggtt agtaccgggc ccgctctaga gatccgacgc 2641 gccatctcta ggcccgcgcc ggccccctcg cacggacttg tgggagaagc tcggctactc 2701 ccctgccccg gttaatttgc atataatatt tcctagtaac tatagaggct taatgtgcga 2761 taaaagacag ataatctgtt ctttttaata ctagctacat tttacatgat aggcttggat 2821 ttctataact tcgtatagca tacattatac gaagttataa acagcacaaa aggaaactca 2881 ccctaactgt aaagtaattg tgtgttttga gactataaGt atcccttgga gaaCCAcctT 2941 GTTGGAAGCC TCGCTGGGGA ACGCCGTTTA AGAGCTATGC TGGAAACAGC ATAGCAAGTT 3001 TAAATAAGGC TAGTCCGTTA TCAACTTGAA AAAGTGGCAC CGAGTCGGTG CTTTTTTTCt 3061 cgagtactag gatccattag gcggccgcgt ggataaccgt attaccgcca tgcattagtt 3121 attaatagta atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta 3181 cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt 3241 caataatgac gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg 3301 tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta 3361 cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga 3421 ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg 3481 tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc 3541 caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact 3601 ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt 3661 gggaggtcta tataagcaga gctggtttag tgaaccgtca gatccgCTAG CCCACCATGG 3721 TGAGCAAGGG CGAGGAGCTG TTCACCGGGG TGGTGCCCAT CCTGGTCGAG CTGGACGGCG 3781 ACGTAAACGG CCACAAGTTC AGCGTGTCTG GCGAGGGCGA GGGCGATGCC ACCTACGGCA 3841 AGCTGACCCT GAAGTTCATC TGCACCACCG GCAAGCTGCC CGTGCCCTGG CCCACCCTCG 3901 TGACCACCCT GACCTACGGC GTGCAGTGCT TCAGCCGCTA CCCCGACCAC ATGAAGCAGC 3961 ACGACTTCTT CAAGTCCGCC ATGCCCGAAG GCTACGTCCA GGAGCGCACC ATCTTCTTCA 4021 AGGACGACGG CAACTACAAG ACCCGCGCCG AGGTGAAGTT CGAGGGCGAC ACCCTGGTGA 4081 ACCGCATCGA GCTGAAGGGC ATCGACTTCA AGGAGGACGG CAACATCCTG GGGCACAAGC 4141 TGGAGTACAA CTACAACAGC CACAACGTCT ATATCATGGC CGACAAGCAG AAGAACGGCA 4201 TCAAGGCGAA CTTCAAGATC CGCCACAACA TCGAGGACGG CAGCGTGCAG CTCGCCGACC 4261 ACTACCAGCA GAACACCCCC ATCGGCGACG GCCCCGTGCT GCTGCCCGAC AACCACTACC 4321 TGAGCACCCA GTCCGCCCTG AGCAAAGACC CCAACGAGAA GCGCGATCAC ATGGTCCTGC 4381 TGGAGTTCGT GACCGCCGCC GGGATCACTC TCGGCATGGA CGAGCTGTAC AAGTAAgaat 4441 tcgtcgaggg acctaataac ttcgtatagc atacattata cgaagttata catgtttaag 4501 ggttccggtt ccactaggta caattcgata tcaagcttat cgataatcaa cctctggatt 4561 acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg 4621 gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct 4681 cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc 4741 aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca 4801 ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac 4861 tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt 4921 ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt gttgccacct 4981 ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc 5041 cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga 5101 cgagtcggat ctccctttgg gccgcctccc cgcatcgata ccgtcgacct cgatcgagac 5161 ctagaaaaac atggagcaat cacaagtagc aatacagcag ctaccaatgc tgattgtgcc 5221 tggctagaag cacaagagga ggaggaggtg ggttttccag tcacacctca ggtaccttta 5281 agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga 5341 ctggaagggc taattcactc ccaacgaaga caagatatcc ttgatctgtg gatctaccac 5401 acacaaggct acttccctga ttggcagaac tacacaccag ggccagggat cagatatcca 5461 ctgacctttg gatggtgcta caagctagta ccagttgagc aagagaaggt agaagaagcc 5521 aatgaaggag agaacacccg cttgttacac cctgtgagcc tgcatgggat ggatgacccg 5581 gagagagaag tattagagtg gaggtttgac agccgcctag catttcatca catggcccga 5641 gagctgcatc cggactgtac tgggtctctc tggttagacc agatctgagc ctgggagctc 5701 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa 5761 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag 5821 tcagtgtgga aaatctctag cagcatgtga gcaaaaggcc agcaaaaggc caggaaccgt 5881 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa 5941 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt 6001 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg 6061 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc 6121 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc 6181 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta 6241 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct 6301 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc 6361 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa 6421 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa 6481 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa 6541 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt 6601 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac 6661 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc 6721 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc 6781 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata 6841 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc 6901 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc 6961 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca 7021 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa 7081 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca 7141 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt 7201 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt 7261 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg 7321 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga 7381 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc 7441 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg 7501 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag 7561 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg 7621 gttccgcgca catttccccg aaaagtgcca cctgac //