LOCUS       pSLQ2853_4_pHR_U        7646 bp ds-DNA     circular     30-SEP-2016
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     U6_sgRNA_template from 1 to 8308
COMMENT     pMig-w from 1 to 6471
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    join(2919..2919,2934..2936,2940..2944)
                     /label=mU6 promoter (modified)
                     /ApEinfo_fwdcolor="#fff431"
                     /ApEinfo_revcolor="#fff431"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             complement(6656..7315)
                     /label=AmpR
                     /ApEinfo_fwdcolor="#ffff00"
                     /ApEinfo_revcolor="#ffff00"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      complement(5876..6558)
                     /label=ColE1 origin
                     /ApEinfo_fwdcolor="gray50"
                     /ApEinfo_revcolor="gray50"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_recomb     2825..2858
                     /label=LoxP recombination site
                     /ApEinfo_fwdcolor="steel blue"
                     /ApEinfo_revcolor="orange"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_recomb     4446..4479
                     /label=LoxP recombination site(1)
                     /ApEinfo_label="LoxP recombination site"
                     /ApEinfo_fwdcolor="steel blue"
                     /ApEinfo_revcolor="orange"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(7555..7583)
                     /label=AmpR promoter
                     /ApEinfo_fwdcolor="#ffff00"
                     /ApEinfo_revcolor="#ffff00"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    844..1024
                     /label=HIV-1_5_LTR
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="cyan"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3707..4426
                     /label=EGFP
                     /ApEinfo_fwdcolor="#00ff00"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    5652..5832
                     /label=HIV-1_5_LTR(1)
                     /ApEinfo_label="HIV-1_5_LTR"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="cyan"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1695..1928
                     /label=RRE
                     /ApEinfo_fwdcolor="#9cffe3"
                     /ApEinfo_revcolor="#9cffe3"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2459..2473
                     /label=cPPT
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="cyan"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    5315..5329
                     /label=cPPT(1)
                     /ApEinfo_label="cPPT"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="cyan"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    4525..5125
                     /label=wPRE
                     /ApEinfo_fwdcolor="#0060ff"
                     /ApEinfo_revcolor="#0060ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(5879..6498)
                     /label=pBR322_origin
                     /ApEinfo_fwdcolor="#95928e"
                     /ApEinfo_revcolor="#95928e"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3106..3688
                     /label=CMV promoter IE
                     /ApEinfo_fwdcolor="#ffed31"
                     /ApEinfo_revcolor="#ffed31"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    263..826
                     /label=pU6 (pMJ177) 5' CMV promoter IE
                     /ApEinfo_fwdcolor="#fffc15"
                     /ApEinfo_revcolor="#fffc15"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3106..3669
                     /label=pU6 (pMJ177) 5' CMV promoter IE(1)
                     /ApEinfo_label="pU6 (pMJ177) 5' CMV promoter IE"
                     /ApEinfo_fwdcolor="#fffc15"
                     /ApEinfo_revcolor="#fffc15"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(1601..1607)
                     /label=TATA box
                     /ApEinfo_fwdcolor="#ff9afa"
                     /ApEinfo_revcolor="#ff9afa"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(766..775)
                     /label=GC Box
                     /ApEinfo_fwdcolor="#888eff"
                     /ApEinfo_revcolor="#888eff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(3609..3618)
                     /label=GC Box(1)
                     /ApEinfo_label="GC Box"
                     /ApEinfo_fwdcolor="#888eff"
                     /ApEinfo_revcolor="#888eff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2966..3048
                     /label=sa optimized scaffold
                     /ApEinfo_fwdcolor="#00ff00"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(5903..5912)
                     /label=GC Box(2)
                     /ApEinfo_label="GC Box"
                     /ApEinfo_fwdcolor="#888eff"
                     /ApEinfo_revcolor="#888eff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2996..2996
                     /label=A->T hairpin flip
                     /ApEinfo_fwdcolor="#ff0000"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2631..2944
                     /label=mU6 promoter (modified)(1)
                     /ApEinfo_label="mU6 promoter (modified)"
                     /ApEinfo_fwdcolor="#fff431"
                     /ApEinfo_revcolor="#fff431"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2969..2969
                     /label=T->A hairpin flip
                     /ApEinfo_fwdcolor="#ff0000"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2945..2965
                     /label=sa sgCXCR4-2
                     /ApEinfo_fwdcolor="#ff80ff"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
ORIGIN
        1 gcttaagcgg tcgacggatc gggagatctc ccgatcccct atggtgcact ctcagtacaa
       61 tctgctctga tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg
      121 ctgagtagtg cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca
      181 tgaagaatct gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata
      241 cgcgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
      301 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
      361 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
      421 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
      481 tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc
      541 ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct
      601 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg
      661 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
      721 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga
      781 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagcgcg ttttgcctgt
      841 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
      901 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
      961 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
     1021 agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac
     1081 gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta
     1141 cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta
     1201 ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga
     1261 aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta
     1321 atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat
     1381 cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca accctctatt
     1441 gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag
     1501 agcaaaacaa aagtaagacc accgcacagc aagcggccgg ccgcgctgat cttcagacct
     1561 ggaggaggag atatgaggga caattggaga agtgaattat ataaatataa agtagtaaaa
     1621 attgaaccat taggagtagc acccaccaag gcaaagagaa gagtggtgca gagagaaaaa
     1681 agagcagtgg gaataggagc tttgttcctt gggttcttgg gagcagcagg aagcactatg
     1741 ggcgcagcgt caatgacgct gacggtacag gccagacaat tattgtctgg tatagtgcag
     1801 cagcagaaca atttgctgag ggctattgag gcgcaacagc atctgttgca actcacagtc
     1861 tggggcatca agcagctcca ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa
     1921 cagctcctgg ggatttgggg ttgctctgga aaactcattt gcaccactgc tgtgccttgg
     1981 aatgctagtt ggagtaataa atctctggaa cagatttgga atcacacgac ctggatggag
     2041 tgggacagag aaattaacaa ttacacaagc ttaatacact ccttaattga agaatcgcaa
     2101 aaccagcaag aaaagaatga acaagaatta ttggaattag ataaatgggc aagtttgtgg
     2161 aattggttta acataacaaa ttggctgtgg tatataaaat tattcataat gatagtagga
     2221 ggcttggtag gtttaagaat agtttttgct gtactttcta tagtgaatag agttaggcag
     2281 ggatattcac cattatcgtt tcagacccac ctcccaaccc cgaggggacc cgacaggccc
     2341 gaaggaatag aagaagaagg tggagagaga gacagagaca gatccattcg attagtgaac
     2401 ggatcggcac tgcgtgcgcc aattctgcag acaaatggca gtattcatcc acaattttaa
     2461 aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac
     2521 agacatacaa actaaagaat tacaaaaaca aattacaaaa attcaaaatt ttcgggttta
     2581 ttacagggac agcagagatc cagtttggtt agtaccgggc ccgctctaga gatccgacgc
     2641 gccatctcta ggcccgcgcc ggccccctcg cacggacttg tgggagaagc tcggctactc
     2701 ccctgccccg gttaatttgc atataatatt tcctagtaac tatagaggct taatgtgcga
     2761 taaaagacag ataatctgtt ctttttaata ctagctacat tttacatgat aggcttggat
     2821 ttctataact tcgtatagca tacattatac gaagttataa acagcacaaa aggaaactca
     2881 ccctaactgt aaagtaattg tgtgttttga gactataaGt atcccttgga gaaCCAcctT
     2941 GTTGGCCCAG GGGACCCTGC TGTTTGTTAT AGTACTCTGG AAACAGAATC TACTATAACA
     3001 AGGCAAAATG CCGTGTTTAT CTCGTCAACT TGTTGGCGAG ATTTTTTTCt cgagtactag
     3061 gatccattag gcggccgcgt ggataaccgt attaccgcca tgcattagtt attaatagta
     3121 atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac
     3181 ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac
     3241 gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt
     3301 acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat
     3361 tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga
     3421 ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt
     3481 ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca
     3541 ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg
     3601 tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta
     3661 tataagcaga gctggtttag tgaaccgtca gatccgCTAG CCCACCATGG TGAGCAAGGG
     3721 CGAGGAGCTG TTCACCGGGG TGGTGCCCAT CCTGGTCGAG CTGGACGGCG ACGTAAACGG
     3781 CCACAAGTTC AGCGTGTCTG GCGAGGGCGA GGGCGATGCC ACCTACGGCA AGCTGACCCT
     3841 GAAGTTCATC TGCACCACCG GCAAGCTGCC CGTGCCCTGG CCCACCCTCG TGACCACCCT
     3901 GACCTACGGC GTGCAGTGCT TCAGCCGCTA CCCCGACCAC ATGAAGCAGC ACGACTTCTT
     3961 CAAGTCCGCC ATGCCCGAAG GCTACGTCCA GGAGCGCACC ATCTTCTTCA AGGACGACGG
     4021 CAACTACAAG ACCCGCGCCG AGGTGAAGTT CGAGGGCGAC ACCCTGGTGA ACCGCATCGA
     4081 GCTGAAGGGC ATCGACTTCA AGGAGGACGG CAACATCCTG GGGCACAAGC TGGAGTACAA
     4141 CTACAACAGC CACAACGTCT ATATCATGGC CGACAAGCAG AAGAACGGCA TCAAGGCGAA
     4201 CTTCAAGATC CGCCACAACA TCGAGGACGG CAGCGTGCAG CTCGCCGACC ACTACCAGCA
     4261 GAACACCCCC ATCGGCGACG GCCCCGTGCT GCTGCCCGAC AACCACTACC TGAGCACCCA
     4321 GTCCGCCCTG AGCAAAGACC CCAACGAGAA GCGCGATCAC ATGGTCCTGC TGGAGTTCGT
     4381 GACCGCCGCC GGGATCACTC TCGGCATGGA CGAGCTGTAC AAGTAAgaat tcgtcgaggg
     4441 acctaataac ttcgtatagc atacattata cgaagttata catgtttaag ggttccggtt
     4501 ccactaggta caattcgata tcaagcttat cgataatcaa cctctggatt acaaaatttg
     4561 tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc
     4621 tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta
     4681 taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt
     4741 ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca
     4801 gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc
     4861 ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt
     4921 gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg
     4981 cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg
     5041 cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat
     5101 ctccctttgg gccgcctccc cgcatcgata ccgtcgacct cgatcgagac ctagaaaaac
     5161 atggagcaat cacaagtagc aatacagcag ctaccaatgc tgattgtgcc tggctagaag
     5221 cacaagagga ggaggaggtg ggttttccag tcacacctca ggtaccttta agaccaatga
     5281 cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc
     5341 taattcactc ccaacgaaga caagatatcc ttgatctgtg gatctaccac acacaaggct
     5401 acttccctga ttggcagaac tacacaccag ggccagggat cagatatcca ctgacctttg
     5461 gatggtgcta caagctagta ccagttgagc aagagaaggt agaagaagcc aatgaaggag
     5521 agaacacccg cttgttacac cctgtgagcc tgcatgggat ggatgacccg gagagagaag
     5581 tattagagtg gaggtttgac agccgcctag catttcatca catggcccga gagctgcatc
     5641 cggactgtac tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac
     5701 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg
     5761 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga
     5821 aaatctctag cagcatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     5881 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     5941 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     6001 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     6061 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     6121 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     6181 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     6241 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     6301 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
     6361 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     6421 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     6481 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     6541 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     6601 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     6661 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     6721 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     6781 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     6841 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     6901 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     6961 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     7021 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     7081 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     7141 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     7201 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     7261 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     7321 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     7381 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     7441 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     7501 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     7561 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     7621 catttccccg aaaagtgcca cctgac   
//
