LOCUS       Exported               14162 bp ds-DNA     circular SYN 04-OCT-2016
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pGIPZ_lenti MS2-P65-HSF1-Hygro_MCS_CMV_VEGF_PC_IRES
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14162)
  AUTHORS   Kelly
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Oct 4, 2016 from SnapGene 3.2.1
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..14162
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          3460..4299
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          4363..4368
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     LTR             1..634
                     /note="3' LTR"
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    454..634
                     /label=HIV-1_5_LTR
                     /note="HIV-1_5_LTR"
     misc_feature    681..806
                     /note="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    745..789
                     /label=HIV-1_psi_pack
                     /note="HIV-1_psi_pack"
     misc_feature    1303..1536
                     /label=TRE
                     /note="RRE"
                     /note="promoter"
     misc_feature    2063..2180
                     /note="cPPT/CTS"
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    2067..2082
                     /label=cPPT
                     /note="cPPT"
                     /note="central polypurine tract helps translocation into 
                     the nucleus of non-dividing cells"
     CDS             complement(2245..2619)
                     /codon_start=1
                     /label=zeoR
                     /note="zeoR"
                     /note="bacterial resistance marker, Zeocin resistance"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     promoter        complement(2629..2647)
                     /note="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(2639..2686)
                     /note="EM7 promoter"
                     /note="synthetic bacterial promoter "
     misc_feature    2738..3180
                     /label=CMV IE
                     /note="CMV IE"
     enhancer        2791..3094
                     /note="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3095..3298
                     /note="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     misc_feature    3315
                     /note="tss"
     CDS             3390
                     /codon_start=1
                     /label=turboGFP
                     /note="turboGFP"
                     /note="marker to track shRNAmir expression,TurboGFP is an 
                     improved variant of the green fluorescent protein CopGFP 
                     cloned from copepod Pontellina plumata  [Shagin et al., 
                     2004]. It possesses bright green fluorescence (excitation/ 
                     emission max = 482/ 502"
                     /translation=""
     CDS             3403..3459
                     /note="leader sequence"
     CDS             3460..3783
                     /note="VL"
     CDS             3784..3828
                     /note="linker"
     CDS             3829..4176
                     /codon_start=1
                     /note="VH"
                     /translation="EVQLVESGGGLVQPGGSLRLSCAASGFTISDYWIHWVRQAPGKGL
                     EWVAGITPAGGYTYYADSVKGRFTISADTSKNTAYLQMNSLRAEDTAVYYCARFVFFLP
                     YAMDYWGQGTLV"
     CDS             4177..4224
                     /codon_start=1
                     /note="Linker"
                     /translation="GAGTGGSGGGTGGSGG"
     misc_feature    4198..4248
                     /note="25"
     misc_feature    4213..4248
                     /note="20"
     CDS             4225..4248
                     /codon_start=1
                     /note="Flag"
                     /translation="DYKDDDDN"
     misc_feature    4225..4248
                     /note="16"
     CDS             4300..4362
                     /codon_start=1
                     /note="CD28_TMD"
                     /translation="LVVVAGVLFCYGLLVTVALCV"
     misc_feature    4363..4368
                     /note="Linker"
     CDS             4369..5073
                     /codon_start=1
                     /note="TEV catalytic"
                     /translation="ESLFKGPRDYNPISSTICHLTNESDGHTTSLYGIGFGPFIITNKH
                     LFRRNNGTLLVQSLHGVFKVKNTTTLQQHLIDGRDMIIIRMPKDFPPFPQKLKFREPQR
                     EERICLVTTNFQTKSMSSMVSDTSCTFPSSDGIFWKHWIQTKDGQCGSPLVSTRDGFIV
                     GIHSASNFTNTNNYFTSVPKNFMELLTNQEAQQWVSGWRLNADSVLWGGHKVFMVKPEE
                     PFQPVKEATQLMN"
     misc_feature    5083..5576
                     /label=IRES
                     /note="IRES"
     misc_feature    5589..5978
                     /note="MS2 Protein"
     CDS             6033..6053
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /note="SV40 NLS"
                     /translation="PKKKRKV"
     misc_feature    6069..6620
                     /note="P65"
     misc_feature    6636..7007
                     /note="HSF1"
     CDS             7023..7076
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /note="T2A"
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     misc_feature    7080..8111
                     /note="Hygro"
     misc_feature    8139..8727
                     /note="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(8610..8621)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /note="Factor Xa site"
                     /translation="IEGR"
     primer_bind     complement(8730..8746)
                     /note="KS primer"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     LTR             8932..9165
                     /note="3' LTR (Delta-U3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     misc_feature    9085..9165
                     /label=HIV-1_3_LTR
                     /note="HIV-1_3_LTR"
     polyA_signal    9272..9496
                     /note="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      9542..9970
                     /direction=RIGHT
                     /note="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        9984..10313
                     /note="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     rep_origin      10164..10299
                     /note="SV40 ori"
                     /note="SV40 origin of replication"
     misc_feature    10164..10241
                     /label=SV40_origin
                     /note="SV40_origin"
     CDS             10362..11387
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /note="HygR"
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     polyA_signal    11517..11638
                     /note="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11687..11703)
                     /note="M13 rev"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    11711..11727
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(11735..11765)
                     /note="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    11780..11801
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding site"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     misc_feature    12074..12693
                     /label=pUCorigin
                     /note="pUCorigin"
     rep_origin      complement(12089..12677)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(12848..13708)
                     /codon_start=1
                     /label=amp R
                     /note="amp R"
                     /note=" -lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(13709..13813)
                     /gene="bla"
                     /note="AmpR promoter"
     polyA_signal    13861..13995
                     /note="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atcggcactg cgtgcgccaa ttctgcagac
     2041 aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg tacagtgcag
     2101 gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa
     2161 ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca gtttggttag
     2221 taccgggccc gctctagtcc ggaatcagtc ctgctcctcg gccacgaagt gcacgcagtt
     2281 gccggccggg tcgcgcaggg cgaactcccg cccccacggc tgctcgccga tctcggtcat
     2341 ggccggcccg gaggcgtccc ggaagttcgt ggacacgacc tccgaccact cggcgtacag
     2401 ctcgtccagg ccgcgcaccc acacccaggc cagggtgttg tccggcacca cctggtcctg
     2461 gaccgcgctg atgaacaggg tcacgtcgtc ccggaccaca ccggcgaagt cgtcctccac
     2521 gaagtcccgg gagaacccga gccggtcggt ccagaactcg accgctccgg cgacgtcgcg
     2581 cgcggtgagc accggaacgg cactggtcaa cttggccatg gtggccctcc tatagtgagt
     2641 cgtattatac tatgccgata tactatgccg atgattaatt gtcaacacgt gctgcaggtc
     2701 cgaggttcta gacgtattac cgccatgcat tagttattaa tagtaatcaa ttacggggtc
     2761 attagttcat agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc
     2821 tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt
     2881 aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca
     2941 cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg
     3001 taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca
     3061 gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa
     3121 tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa
     3181 tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc
     3241 cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg
     3301 tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag
     3361 acaccgactc tactagagga tctgccacca gctagcgcca tcatggcttg gaccagctta
     3421 attcttagct tgcttgccct gtgcagcggt gcatcatctg acatacagat gactcagtca
     3481 cctagcagtc tgtctgcttc agtcggtgat agggtaacta tcacctgtcg ggcatctcag
     3541 gatgtgtcca cggcagttgc ctggtaccag cagaagcccg gcaaggcgcc taagttgctt
     3601 atctattcag cttcattttt gtatagtggg gtgcctagcc ggtttagcgg aagtggttct
     3661 ggcacagact tcactttgac aatcagttcc ctccaaccgg aggatttcgc tacctactac
     3721 tgtcagcagg ggtacggaaa cccctttacg tttggacagg gcactaaagt ggagattaag
     3781 aggggaggcg gaggctccgg tgggggaggc tctggtggtg gaggtagcga ggttcagctg
     3841 gtggaaagtg gtggcggact agtacagcca ggcggctccc tgcggctgag ttgcgccgcg
     3901 agtggcttta ctatatctga ctattggatc cattgggtca ggcaagcccc tgggaaagga
     3961 ttggagtggg ttgctggtat tacccccgca ggtgggtata cttattatgc agatagcgtg
     4021 aaagggaggt tcaccatctc tgcagacaca agcaagaaca cggcctacct acagatgaac
     4081 tctctgaggg cagaggacac tgccgtctac tattgcgccc gatttgtgtt ttttctaccc
     4141 tacgccatgg actattgggg acaggggaca ttggtaggcg ccgggacagg agggtctggg
     4201 ggtggcactg gcggatctgg aggagactac aaggacgatg acgacaacgg cggatcgggg
     4261 gggtctggtg gtagtggcgg ttcgggggga ggtaccggtc tggtcgtggt tgctggagtc
     4321 ctgttttgtt atggcttgct agtgacagtg gctctttgtg ttgaaaacga gagcttgttt
     4381 aaggggccgc gtgattacaa cccgatatcg agcaccattt gtcatttgac gaatgaatct
     4441 gatgggcaca caacatcgtt gtatggtatt ggatttggtc ccttcatcat tacaaacaag
     4501 cacttgttta gaagaaataa tggaacactg ttggtccaat cactacatgg tgtattcaag
     4561 gtcaagaaca ccacgacttt gcaacaacac ctcattgatg ggagggacat gataattatt
     4621 cgcatgccta aggatttccc accatttcct caaaagctga aatttagaga gccacaaagg
     4681 gaagagcgca tatgtcttgt gacaaccaac ttccaaacta agagcatgtc tagcatggtg
     4741 tcagacacta gttgcacatt cccttcatct gatggcatat tctggaagca ttggattcaa
     4801 accaaggatg ggcagtgtgg cagtccatta gtatcaacta gagatgggtt cattgttggt
     4861 atacactcag catcgaattt caccaacaca aacaattatt tcacaagcgt gccgaaaaac
     4921 ttcatggaat tgttgacaaa tcaggaggcg cagcagtggg ttagtggttg gcgattaaat
     4981 gctgactcag tattgtgggg gggccataaa gttttcatgg tgaaacctga agagcctttt
     5041 cagccagtta aggaagcgac tcaactcatg aatggcgcgc ctgttatttt ccaccatatt
     5101 gccgtctttt ggcaatgtga gggcccggaa acctggccct gtcttcttga cgagcattcc
     5161 taggggtctt tcccctctcg ccaaaggaat gcaaggtctg ttgaatgtcg tgaaggaagc
     5221 agttcctctg gaagcttctt gaagacaaac aacgtctgta gcgacccttt gcaggcagcg
     5281 gaacccccca cctggcgaca ggtgcctctg cggccaaaag ccacgtgtat aagatacacc
     5341 tgcaaaggcg gcacaacccc agtgccacgt tgtgagttgg atagttgtgg aaagagtcaa
     5401 atggctctcc tcaagcgtat tcaacaaggg gctgaaggat gcccagaagg taccccattg
     5461 tatgggatct gatctggggc ctcggtgcac atgctttaca tgtgtttagt cgaggttaaa
     5521 aaaacgtcta ggccccccga accacgggga cgtggttttc ctttgaaaaa cacgatcgta
     5581 cggccaccat ggcttcaaac tttactcagt tcgtgctcgt ggacaatggt gggacagggg
     5641 atgtgacagt ggctccttct aatttcgcta atggggtggc agagtggatc agctccaact
     5701 cacggagcca ggcctacaag gtgacatgca gcgtcaggca gtctagtgcc cagaagagaa
     5761 agtataccat caaggtggag gtccccaaag tggctaccca gacagtgggc ggagtcgaac
     5821 tgcctgtcgc cgcttggagg tcctacctga acatggagct cactatccca attttcgcta
     5881 ccaattctga ctgtgaactc atcgtgaagg caatgcaggg gctcctcaaa gacggtaatc
     5941 ctatcccttc cgccatcgcc gctaactcag gtatctacag cgctggagga ggtggaagcg
     6001 gaggaggagg aagcggagga ggaggtagcg gacctaagaa aaagaggaag gtggcggccg
     6061 ctggatcccc ttcagggcag atcagcaacc aggccctggc tctggcccct agctccgctc
     6121 cagtgctggc ccagactatg gtgccctcta gtgctatggt gcctctggcc cagccacctg
     6181 ctccagcccc tgtgctgacc ccaggaccac cccagtcact gagcgctcca gtgcccaagt
     6241 ctacacaggc cggcgagggg actctgagtg aagctctgct gcacctgcag ttcgacgctg
     6301 atgaggacct gggagctctg ctggggaaca gcaccgatcc cggagtgttc acagatctgg
     6361 cctccgtgga caactctgag tttcagcagc tgctgaatca gggcgtgtcc atgtctcata
     6421 gtacagccga accaatgctg atggagtacc ccgaagccat tacccggctg gtgaccggca
     6481 gccagcggcc ccccgacccc gctccaactc ccctgggaac cagcggcctg cctaatgggc
     6541 tgtccggaga tgaagacttc tcaagcatcg ctgatatgga ctttagtgcc ctgctgtcac
     6601 agatttcctc tagtgggcag ggaggaggtg gaagcggctt cagcgtggac accagtgccc
     6661 tgctggacct gttcagcccc tcggtgaccg tgcccgacat gagcctgcct gaccttgaca
     6721 gcagcctggc cagtatccaa gagctcctgt ctccccagga gccccccagg cctcccgagg
     6781 cagagaacag cagcccggat tcagggaagc agctggtgca ctacacagcg cagccgctgt
     6841 tcctgctgga ccccggctcc gtggacaccg ggagcaacga cctgccggtg ctgtttgagc
     6901 tgggagaggg ctcctacttc tccgaagggg acggcttcgc cgaggacccc accatctccc
     6961 tgctgacagg ctcggagcct cccaaagcca aggaccccac tgtctcctgt acaggcagtg
     7021 gagagggcag aggaagtctg ctaacatgcg gtgacgtcga ggagaatcct ggcccaacca
     7081 tgaaaaagcc tgaactcacc gctacctctg tcgagaagtt tctgatcgaa aagttcgaca
     7141 gcgtctccga cctgatgcag ctctccgagg gcgaagaatc tcgggctttc agcttcgatg
     7201 tgggagggcg tggatatgtc ctgcgggtga atagctgcgc cgatggtttc tacaaagatc
     7261 gctatgttta tcggcacttt gcatccgccg ctctccctat tcccgaagtg cttgacattg
     7321 gggagttcag cgagagcctg acctattgca tctcccgccg tgcacagggt gtcaccttgc
     7381 aagacctgcc tgaaaccgaa ctgcccgctg ttctccagcc cgtcgccgag gccatggatg
     7441 ccatcgctgc cgccgatctt agccagacca gcgggttcgg cccattcgga cctcaaggaa
     7501 tcggtcaata cactacatgg cgcgatttca tctgcgctat tgctgatccc catgtgtatc
     7561 actggcaaac tgtgatggac gacaccgtca gtgcctccgt cgcccaggct ctcgatgagc
     7621 tgatgctttg ggccgaggac tgccccgaag tccggcacct cgtgcacgcc gatttcggct
     7681 ccaacaatgt cctgaccgac aatggccgca taacagccgt cattgactgg agcgaggcca
     7741 tgttcgggga ttcccaatac gaggtcgcca acatcttctt ctggaggccc tggttggctt
     7801 gtatggagca gcagacccgc tacttcgagc ggaggcatcc cgagcttgca ggatctcctc
     7861 ggctccgggc ttatatgctc cgcattggtc ttgaccaact ctatcagagc ttggttgacg
     7921 gcaatttcga tgatgcagct tgggctcagg gtcgctgcga cgcaatcgtc cggtccggag
     7981 ccgggactgt cgggcgtaca caaatcgccc gcagaagcgc tgccgtctgg accgatggct
     8041 gtgtggaagt gctcgccgat agtggaaaca gacgccccag cactcgtcct agggcaaagg
     8101 atctgcagta atgagaattc gatatcaagc ttatcggtaa tcaacctctg gattacaaaa
     8161 tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg
     8221 ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct
     8281 tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg
     8341 gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct
     8401 gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg
     8461 ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg
     8521 tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc acctggattc
     8581 tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc
     8641 gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc
     8701 ggatctccct ttgggccgcc tccccgcatc gataccgtcg acctcgagac ctagaaaaac
     8761 atggagcaat cacaagtagc aatacagcag ctaccaatgc tgattgtgcc tggctagaag
     8821 cacaagagga ggaggaggtg ggttttccag tcacacctca ggtaccttta agaccaatga
     8881 cttacaaggc agctgtagat cttagccact ttttaaaaga aaagagggga ctggaagggc
     8941 taattcactc ccaacgaaga caagatctgc tttttgcttg tactgggtct ctctggttag
     9001 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
     9061 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
     9121 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc
     9181 atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggcctt
     9241 gacattgttt aaacccgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt
     9301 gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc
     9361 taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt
     9421 ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat
     9481 gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctctag ggggtatccc
     9541 cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
     9601 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
     9661 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
     9721 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
     9781 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
     9841 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
     9901 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
     9961 aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc
    10021 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt
    10081 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
    10141 tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc
    10201 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tctgcctctg
    10261 agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc
    10321 cgggagcttg tatatccatt ttcggatctg atcagcacgt gatgaaaaag cctgaactca
    10381 ccgcgacgtc tgtcgagaag tttctgatcg aaaagttcga cagcgtctcc gacctgatgc
    10441 agctctcgga gggcgaagaa tctcgtgctt tcagcttcga tgtaggaggg cgtggatatg
    10501 tcctgcgggt aaatagctgc gccgatggtt tctacaaaga tcgttatgtt tatcggcact
    10561 ttgcatcggc cgcgctcccg attccggaag tgcttgacat tggggaattc agcgagagcc
    10621 tgacctattg catctcccgc cgtgcacagg gtgtcacgtt gcaagacctg cctgaaaccg
    10681 aactgcccgc tgttctgcag ccggtcgcgg aggccatgga tgcgatcgct gcggccgatc
    10741 ttagccagac gagcgggttc ggcccattcg gaccgcaagg aatcggtcaa tacactacat
    10801 ggcgtgattt catatgcgcg attgctgatc cccatgtgta tcactggcaa actgtgatgg
    10861 acgacaccgt cagtgcgtcc gtcgcgcagg ctctcgatga gctgatgctt tgggccgagg
    10921 actgccccga agtccggcac ctcgtgcacg cggatttcgg ctccaacaat gtcctgacgg
    10981 acaatggccg cataacagcg gtcattgact ggagcgaggc gatgttcggg gattcccaat
    11041 acgaggtcgc caacatcttc ttctggaggc cgtggttggc ttgtatggag cagcagacgc
    11101 gctacttcga gcggaggcat ccggagcttg caggatcgcc gcggctccgg gcgtatatgc
    11161 tccgcattgg tcttgaccaa ctctatcaga gcttggttga cggcaatttc gatgatgcag
    11221 cttgggcgca gggtcgatgc gacgcaatcg tccgatccgg agccgggact gtcgggcgta
    11281 cacaaatcgc ccgcagaagc gcggccgtct ggaccgatgg ctgtgtagaa gtactcgccg
    11341 atagtggaaa ccgacgcccc agcactcgtc cgagggcaaa ggaatagcac gtgctacgag
    11401 atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg
    11461 ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccaact
    11521 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
    11581 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
    11641 atgtctgtat accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc
    11701 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
    11761 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
    11821 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
    11881 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
    11941 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
    12001 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
    12061 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
    12121 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
    12181 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
    12241 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
    12301 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
    12361 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
    12421 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
    12481 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
    12541 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
    12601 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
    12661 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
    12721 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
    12781 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
    12841 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
    12901 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
    12961 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
    13021 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
    13081 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
    13141 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
    13201 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
    13261 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
    13321 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
    13381 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
    13441 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
    13501 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
    13561 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
    13621 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
    13681 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
    13741 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
    13801 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtcgacgga tcgggagatc
    13861 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
    13921 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
    13981 tatcatgtct ggatcaactg gataactcaa gctaaccaaa atcatcccaa acttcccacc
    14041 ccatacccta ttaccactgc caattacctg tggtttcatt tactctaaac ctgtgattcc
    14101 tctgaattat tttcatttta aagaaattgt atttgttaaa tatgtactac aaacttagta
    14161 gt
//
