LOCUS       Exported                4739 bp ds-DNA     circular SYN 20-DEC-2016
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    84553
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4739)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Dec 20, 2016 from SnapGene 3.2.1
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4739
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     misc_feature    1..573
                     /label=bGlob int
                     /note="bGlob int"
     terminator      625..1142
                     /label=rb_glob_PA
                     /note="rb_glob_PA"
     rep_origin      complement(1376..1995)
                     /direction=LEFT
                     /label=pBR322 origin
                     /note="pBR322 origin"
     CDS             complement(2150..3010)
                     /codon_start=1
                     /label=AmpR
                     /note="AmpR"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3052..3080)
                     /label=AmpR promoter
                     /note="AmpR promoter"
     terminator      3549..3680
                     /label=SV40 terminator
                     /note="SV40 terminator"
     CDS             complement(3717..4436)
                     /codon_start=1
                     /product="enhanced YFP"
                     /note="EYFP"
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     misc_feature    3762..3783
                     /label=EGFP_C_primer
                     /note="EGFP_C_primer"
     misc_feature    4370..4391
                     /label=EGFP_N_primer
                     /note="EGFP_N_primer"
     promoter        complement(4482..4577)
                     /label=CMV2
                     /note="CMV2"
     protein_bind    4584..4602
                     /gene="tetO"
                     /bound_moiety="tetracycline repressor TetR"
                     /note="tet operator"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4603..4621
                     /gene="tetO"
                     /bound_moiety="tetracycline repressor TetR"
                     /note="tet operator"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     misc_feature    4622..4638
                     /note="UAS"
     misc_feature    4639..4655
                     /note="UAS"
     misc_feature    4662..4678
                     /note="UAS"
     misc_feature    4679..4695
                     /note="UAS"
     protein_bind    4696..4714
                     /gene="tetO"
                     /bound_moiety="tetracycline repressor TetR"
                     /note="tet operator"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4715..4733
                     /gene="tetO"
                     /bound_moiety="tetracycline repressor TetR"
                     /note="tet operator"
                     /note="bacterial operator O2 for the tetR and tetA genes"
ORIGIN
        1 gtgagtttgg ggacccttga ttgttctttc tttttcgcta ttgaaaaatt catgttatat
       61 ggagggggca aagttttcag ggtgttgttt agaatgggaa gatgtccctt gtatcaccat
      121 ggaccctcat gataattttg tttctttcac tttctactct gttgacaacc attgtctcct
      181 cttattttct tttcattttc tgtaactttt ttcgttaaac tttagcttgc atttgtaacg
      241 aatttttaaa ttcactttcg tttatttgtc agattgtaag tactttctct aatcactttt
      301 ttttcaaggc aatcagggta attatattgt acttcagcac agttttagag aacaattgtt
      361 ataattaaat gataaggtag aatatttctg catataaatt ctggctggcg tggaaatatt
      421 cttattggta gaaacaacta catcctggta atcatcctgc ctttctcttt atggttacaa
      481 tgatatacac tgtttgagat gaggataaaa tactctgagt ccaaaccggg cccctctgct
      541 aaccatgttc atgccttctt ctttttccta cagctcctgg gcaacgtgct ggttgttgtg
      601 ctgtctcatc attttggcaa agaattcact cctcaggtgc aggctgccta tcagaaggtg
      661 gtggctggtg tggccaatgc cctggctcac aaataccact gagatcgatc tttttccctc
      721 tgccaaaaat tatggggaca tcatgaagcc ccttgagcat ctgacttctg ggtaataaag
      781 gaaatttatt ttcattgcaa tagtgtgtgg gaattttttg tgtctctcac tcggaaggac
      841 atatgggagg gcaaatcatt taaaacatca gaatgagtat ttggtttaga gtttggcaac
      901 atatgccata tgctggctgc catgaacaaa ggtggctata aagaggtcat cagtatatga
      961 aacagccccc tgctgtccat tccttattcc atagaaaagc cttgacttga ggttagattt
     1021 tttttatatt ttgttttgtg ttattttttt ctttaacatc cctaaaattt tccttacatg
     1081 ttttactagc cagatttttc ctcctctcct gactactccc agtcatagct gtccctcttc
     1141 tcttatgaac tcgactgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     1201 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     1261 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     1321 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     1381 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     1441 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     1501 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     1561 cttcgggaag cgtggcgctt tctcaatgct cacgctgtag gtatctcagt tcggtgtagg
     1621 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     1681 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     1741 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     1801 agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga
     1861 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     1921 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     1981 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     2041 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     2101 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     2161 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     2221 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     2281 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     2341 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     2401 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     2461 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     2521 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     2581 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     2641 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     2701 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     2761 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     2821 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     2881 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     2941 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     3001 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     3061 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     3121 ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata
     3181 aaaataggcg tatcacgagg ccctttcgtc ttcactcgac gctggtcgag ctgatacttc
     3241 ccgtccgcca ggggacatgc cggcgatgct gaaggtcgcg cgcattcccg atgaagaggc
     3301 cggttaccgc ctgttgacct ggtgggacgg gcagggcgcc gcccgagtct tgcctcggcg
     3361 gcgggcgctc tgctcatgga gcgcgcgtcc ggggaccttg cacagatagc gtggtccggc
     3421 cagacgacga ggcttgcagg atcataatca gccataccac atttgtagag gttttacttg
     3481 ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg
     3541 ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     3601 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     3661 tatcttatca tgtctggatc ctctagaact aggtcgacaa gcttgatatc gaattcttac
     3721 ttgtacagct cgtccatgcc gagagtgatc ccggcggcgg tcacgaactc cagcaggacc
     3781 atgtgatcgc gcttctcgtt ggggtctttg ctcagggcgg actggtagct caggtagtgg
     3841 ttgtcgggca gcagcacggg gccgtcgccg atgggggtgt tctgctggta gtggtcggcg
     3901 agctgcacgc tgccgtcctc gatgttgtgg cggatcttga agttcacctt gatgccgttc
     3961 ttctgcttgt cggccatgat atagacgttg tggctgttgt agttgtactc cagcttgtgc
     4021 cccaggatgt tgccgtcctc cttgaagtcg atgcccttca gctcgatgcg gttcaccagg
     4081 gtgtcgccct cgaacttcac ctcggcgcgg gtcttgtagt tgccgtcgtc cttgaagaag
     4141 atggtgcgct cctggacgta gccttcgggc atggcggact tgaagaagtc gtgctgcttc
     4201 atgtggtcgg ggtagcgggc gaagcactgc aggccgtagc cgaaggtggt cacgagggtg
     4261 ggccagggca cgggcagctt gccggtggtg cagatgaact tcagggtcag cttgccgtag
     4321 gtggcatcgc cctcgccctc gccggacacg ctgaacttgt ggccgtttac gtcgccgtcc
     4381 agctcgacca ggatgggcac caccccggtg aacagctcct cgcccttgct caccatacta
     4441 gcgcggcctc gacgatatct ctagagctga gaacttcagg caggctggat cggtcccggt
     4501 gtcttctatg gaggtcaaaa cagcgtggat ggcgtctcca ggcgatctga cggttcacta
     4561 aacgagctct gcttataggt acctccctat cagtgataga gatccctatc agtgatagag
     4621 acggagtact gtcctccgcg gagtactgtc ctccgaccgg ccggagtact gtcctccgcg
     4681 gagtactgtc ctccgtccct atcagtgata gagatcccta tcagtgatag agaaccggt
//
