LOCUS Exported 6885 bp ds-DNA circular SYN 11-JAN-2017 DEFINITION synthetic circular DNA. ACCESSION . VERSION . KEYWORDS Rap-MESA.FKBP20.Tev SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6885) AUTHORS Leonard Lab TITLE Direct Submission JOURNAL Exported Wednesday, Jan 11, 2017 from SnapGene 3.3.1 http://www.snapgene.com FEATURES Location/Qualifiers source 1..6885 /organism="synthetic DNA construct" /mol_type="other DNA" repeat_region 1..79 /note="ITR" promoter 172..759 /label=CMV promoter /note="CMV promoter" /note="Unknown feature type:Promoter" CDS 847..924 /codon_start=1 /label=CD4 signal sequence /note="CD4 signal sequence" /translation="MCRAISLRRLLLLLLQLSQLLAVTQG" CDS 925..1374 /codon_start=1 /note="FKBP" /translation="MGVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNK PFKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVEL LKLESAGGSAGGSAGGSAGGSAGGPRAQASNSAVDGTAGPGSTGSR" misc_feature 925..927 /label=START SITE /note="START SITE" CDS 1435..1497 /codon_start=1 /note="CD28_TMD" /translation="LVVVAGVLFCYGLLVTVALCV" misc_feature 1498..1503 /note="Linker" CDS 1504..2208 /codon_start=1 /note="TEV catalytic" /translation="ESLFKGPRDYNPISSTICHLTNESDGHTTSLYGIGFGPFIITNKH LFRRNNGTLLVQSLHGVFKVKNTTTLQQHLIDGRDMIIIRMPKDFPPFPQKLKFREPQR EERICLVTTNFQTKSMSSMVSDTSCTFPSSDGIFWKHWIQTKDGQCGSPLVSTRDGFIV GIHSASNFTNTNNYFTSVPKNFMELLTNQEAQQWVSGWRLNADSVLWGGHKVFMVKPEE PFQPVKEATQLMN" polyA_site 2332..2724 /note="beta-globin poly(A)" /note="human beta-globin polyadenylation signal" repeat_region 3105..3212 /note="ITR" misc_feature 3286 /note="Fake EcoRI site" rep_origin 3632..4087 /direction=RIGHT /note="f1 ori" /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" misc_feature 4556 /note="Fake EcoRI site" promoter 4891..4995 /gene="bla" /note="AmpR promoter" CDS 4996..5856 /codon_start=1 /gene="bla" /product="beta-lactamase" /note="AmpR" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" rep_origin 6027..6615 /direction=RIGHT /note="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" repeat_region 6855..6885 /note="ITR" ORIGIN 1 gggcaaagcc cgggcgtcgg gcgacctttg gtcgcccggc ctcagtgagc gagcgagcgc 61 gcagagaggg agtggccaac tccatcacta ggggttcctt gtagttaatg attaacccgc 121 catgctactt atctacgtag ccatgctcta gggatccggc cagatatacg cgttgacatt 181 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 241 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 301 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 361 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 421 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 481 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 541 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 601 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 661 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 721 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 781 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 841 gccatcatgt gccgagccat ctctcttagg cgcttgctgc tgctgctgct gcagctgtca 901 caactcctag ctgtcactca agggatggga gtgcaggtgg aaaccatctc cccaggagac 961 gggcgcacct tccccaagcg cggccagacc tgcgtggtgc actacaccgg gatgcttgaa 1021 gatggaaaga aatttgattc ctcccgggac agaaacaagc cctttaagtt tatgctaggc 1081 aagcaggagg tgatccgagg ctgggaagaa ggggttgccc agatgagtgt gggtcagaga 1141 gccaaactga ctatatctcc agattatgcc tatggtgcca ctgggcaccc aggcatcatc 1201 ccaccacatg ccactctcgt cttcgatgtg gagcttctaa aactggaaag tgctggtggt 1261 agtgctggtg gtagtgctgg tggtagtgct ggtggtagtg ctggtggtcc tcgagctcaa 1321 gcttcgaatt ctgcagtcga cggtaccgcg ggcccgggat ccaccggatc tagaggcgga 1381 tctggaggct ctgggggttc aggcggatca ggaggcagtg gcgggtcaac cggtctggtc 1441 gtggttgctg gagtcctgtt ttgttatggc ttgctagtga cagtggctct ttgtgttgaa 1501 aacgagagct tgtttaaggg gccgcgtgat tacaacccga tatcgagcac catttgtcat 1561 ttgacgaatg aatctgatgg gcacacaaca tcgttgtatg gtattggatt tggtcccttc 1621 atcattacaa acaagcactt gtttagaaga aataatggaa cactgttggt ccaatcacta 1681 catggtgtat tcaaggtcaa gaacaccacg actttgcaac aacacctcat tgatgggagg 1741 gacatgataa ttattcgcat gcctaaggat ttcccaccat ttcctcaaaa gctgaaattt 1801 agagagccac aaagggaaga gcgcatatgt cttgtgacaa ccaacttcca aactaagagc 1861 atgtctagca tggtgtcaga cactagttgc acattccctt catctgatgg catattctgg 1921 aagcattgga ttcaaaccaa ggatgggcag tgtggcagtc cattagtatc aactagagat 1981 gggttcattg ttggtataca ctcagcatcg aatttcacca acacaaacaa ttatttcaca 2041 agcgtgccga aaaacttcat ggaattgttg acaaatcagg aggcgcagca gtgggttagt 2101 ggttggcgat taaatgctga ctcagtattg tgggggggcc ataaagtttt catggtgaaa 2161 cctgaagagc cttttcagcc agttaaggaa gcgactcaac tcatgaatta gtagcggccg 2221 ccaccgcggt ggagctgttt aaacctcgag caattcaccc caccagtgca ggctgcctat 2281 cagaaagtgg tggctggtgt ggctaatgcc ctggcccaca agtatcacta agctcgcttt 2341 cttgctgtcc aatttctatt aaaggttcct ttgttcccta agtccaacta ctaaactggg 2401 gatattatga agggccttga gcatctggat tctgcctaat aaaaaacatt tattttcatt 2461 gcaatgatgt atttaaatta tttctgaata ttttactaaa aagggaatgt gggaggtcag 2521 tgcatttaaa acataaagaa atgaagagct agttcaaacc ttgggaaaat acactatatc 2581 ttaaactcca tgaaagaagg tgaggctgca aacagtaatg cacattggca acagcccctg 2641 atgcctatgc cttattcatc cctcagaaaa ggattcaagt agaggcttga tttggaggtt 2701 aaagttttgc tatgctgtat tttacattac ttattgtttt agctgtcctc atgaatgtct 2761 tttcactacc catttgctta tcctgcatct ctcagccttg actccactca gttctcttgc 2821 ttagagatac cacctttccc ctgaagtgtt ccttccatgt tttacggcga gatggtttct 2881 cctcgcctgg ccactcagcc ttagttgtct ctgttgtctt atagaggtct acttgaagaa 2941 ggaaaaacag ggggcatggt ttgactgtcc tgtgagccct tcttccctgc ctcccccact 3001 cacagtgacc cggaatccct cgacatggca gtctagagca tggctacgta gataagtagc 3061 atggcgggtt aatcattaac tacaaggaac ccctagtgat ggagttggcc actccctctc 3121 tgcgcgctcg ctcgctcact gaggccgggc gaccaaaggt cgcccgacgc ccgggctttg 3181 cccgggcggc ctcagtgagc gagcgagcgc gccagctggc gtaatagcga agaggcccgc 3241 accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatggaattc cagacgattg 3301 agcgtcaaaa tgtaggtatt tccatgagcg tttttcctgt tgcaatggct ggcggtaata 3361 ttgttctgga tattaccagc aaggccgata gtttgagttc ttctactcag gcaagtgatg 3421 ttattactaa tcaaagaagt attgcgacaa cggttaattt gcgtgatgga cagactcttt 3481 tactcggtgg cctcactgat tataaaaaca cttctcagga ttctggcgta ccgttcctgt 3541 ctaaaatccc tttaatcggc ctcctgttta gctcccgctc tgattctaac gaggaaagca 3601 cgttatacgt gctcgtcaaa gcaaccatag tacgcgccct gtagcggcgc attaagcgcg 3661 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct 3721 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta 3781 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa 3841 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct 3901 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc 3961 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg 4021 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgttt 4081 acaatttaaa tatttgctta tacaatcttc ctgtttttgg ggcttttctg attatcaacc 4141 ggggtacata tgattgacat gctagtttta cgattaccgt tcatcgattc tcttgtttgc 4201 tccagactct caggcaatga cctgatagcc tttgtagaga cctctcaaaa atagctaccc 4261 tctccggcat gaatttatca gctagaacgg ttgaatatca tattgatggt gatttgactg 4321 tctccggcct ttctcacccg tttgaatctt tacctacaca ttactcaggc attgcattta 4381 aaatatatga gggttctaaa aatttttatc cttgcgttga aataaaggct tctcccgcaa 4441 aagtattaca gggtcataat gtttttggta caaccgattt agctttatgc tctgaggctt 4501 tattgcttaa ttttgctaat tctttgcctt gcctgtatga tttattggat gttggaattc 4561 ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atggtgcact 4621 ctcagtacaa tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc 4681 gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc 4741 gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgagacga 4801 aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag 4861 acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa 4921 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat 4981 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg 5041 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa 5101 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt 5161 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt 5221 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat 5281 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg 5341 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta 5401 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat 5461 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag 5521 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa 5581 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca 5641 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc 5701 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt 5761 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc 5821 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat 5881 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt 5941 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac 6001 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc 6061 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca 6121 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta 6181 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct 6241 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg 6301 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc 6361 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta 6421 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg 6481 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt 6541 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg 6601 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg 6661 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc 6721 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg 6781 agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt 6841 cattaatgca gcagctgcgc gctcgctcgc tcactgaggc cgccc //