LOCUS pcDNA3-ERKsubDD-Venus-NES 6342 bp DNA circular 10/6/16 DEFINITION ACCESSION VERSION SOURCE ORGANISM COMMENT Serial Cloner Genbank Format COMMENT SerialCloner_Type=Degen. DNA COMMENT SerialCloner_Comments= COMMENT SerialCloner_Ends=0,0,,0, FEATURES Location/Qualifiers misc_feature 1057..1110 /label=ERKsub_DD /SerialCloner_Color=&h999999 /SerialCloner_Show=True /SerialCloner_Protect=True /SerialCloner_Arrow=True /SerialCloner_Desc=note="direct strand translation" \ /SerialCloner_Desc=translation="PDVPRTPVGKAKLSFQFP" \ CDS 1126..1842 /label=Venus /SerialCloner_Color=&hFFFF00 /SerialCloner_Show=True /SerialCloner_Protect=False /SerialCloner_Arrow=True primer_bind complement(4181..4201) /label=M13-rev /SerialCloner_Color=&h008B8B /SerialCloner_Show=True /SerialCloner_Protect=False /SerialCloner_Arrow=True primer_bind 863..882 /label=T7 /SerialCloner_Color=&h008B8B /SerialCloner_Show=True /SerialCloner_Protect=False /SerialCloner_Arrow=True primer_bind complement(1895..1912) /label=SP6 /SerialCloner_Color=&h008B8B /SerialCloner_Show=True /SerialCloner_Protect=False /SerialCloner_Arrow=True rep_origin complement(4569..5197) /label=ColE1 origin /SerialCloner_Color=&h4682B4 /SerialCloner_Show=True /SerialCloner_Protect=False /SerialCloner_Arrow=True misc_binding complement(4207..4229) /label=LacO /SerialCloner_Color=&h6495ED /SerialCloner_Show=True /SerialCloner_Protect=False /SerialCloner_Arrow=True CDS complement(5349..6008) /label=AmpR /SerialCloner_Color=&h8FBC8F /SerialCloner_Show=True /SerialCloner_Protect=False /SerialCloner_Arrow=True CDS 3050..3841 /label=Kan/neoR /SerialCloner_Color=&h8FBC8F /SerialCloner_Show=True /SerialCloner_Protect=False /SerialCloner_Arrow=True Promoter complement(6248..6276) /label=Amp prom /SerialCloner_Color=&h4682B4 /SerialCloner_Show=True /SerialCloner_Protect=False /SerialCloner_Arrow=True Promoter complement(4234..4263) /label=lac /SerialCloner_Color=&h4682B4 /SerialCloner_Show=True /SerialCloner_Protect=False /SerialCloner_Arrow=True misc_feature 2831..2908 /label=SV40 ORI /SerialCloner_Color=&h4682B4 /SerialCloner_Show=True /SerialCloner_Protect=False /SerialCloner_Arrow=True terminator 1935..2162 /label=bGH PA /SerialCloner_Color=&hFF8000 /SerialCloner_Show=True /SerialCloner_Protect=False /SerialCloner_Arrow=True misc_feature 232..819 /label=CMV prom /SerialCloner_Color=&h800080 /SerialCloner_Show=True /SerialCloner_Protect=False /SerialCloner_Arrow=True CDS 922..939 /label=6His /SerialCloner_Color=&h008000 /SerialCloner_Show=True /SerialCloner_Protect=False /SerialCloner_Arrow=True misc_feature 1849..1875 /label=NES-Targeting /SerialCloner_Color=&h800040 /SerialCloner_Show=True /SerialCloner_Protect=False /SerialCloner_Arrow=True ORIGIN 1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggAGACCCAA GCTTGCGGCC 901 GCCACCATGG TGCGGGGTTC TCATCATCAT CATCATCATG GTATGGCTAG CATGACTGGT 961 GGACAGCAAA TGGGTCGGGA TCTGTACGAC GATGACGATA AGGATCCcAT GGCTGACCAA 1021 CTGACTGAAG AGTGGTCGAC GGTACCGCGG ACCGGTCCTG ACGTACCACG TACACCAGTA 1081 GGAAAGGCAA AGCTGTCATT CCAATTCCCC CGCGCACGGG AGCTCATGGT GAGCAAGGGC 1141 GAGGAGCTGT TCACCGGGGT GGTGCCCATC CTGGTCGAGC TGGACGGCGA CGTAAACGGC 1201 CACAAGTTCA GCGTGTCCGG CGAGGGCGAG GGCGATGCCA CCTACGGCAA GCTGACCCTG 1261 AAGCTGATCT GCACCACCGG CAAGCTGCCC GTGCCCTGGC CCACCCTCGT GACCACCCTG 1321 GGCTACGGCC TGCAGTGCTT CGCCCGCTAC CCCGACCACA TGAAGCAGCA CGACTTCTTC 1381 AAGTCCGCCA TGCCCGAAGG CTACGTCCAG GAGCGCACCA TCTTCTTCAA GGACGACGGC 1441 AACTACAAGA CCCGCGCCGA GGTGAAGTTC GAGGGCGACA CCCTGGTGAA CCGCATCGAG 1501 CTGAAGGGCA TCGACTTCAA GGAGGACGGC AACATCCTGG GGCACAAGCT GGAGTACAAC 1561 TACAACAGCC ACAACGTCTA TATCACCGCC GACAAGCAGA AGAACGGCAT CAAGGCCAAC 1621 TTCAAGATCC GCCACAACAT CGAGGACGGC GGCGTGCAGC TCGCCGACCA CTACCAGCAG 1681 AACACCCCCA TCGGCGACGG CCCCGTGCTG CTGCCCGACA ACCACTACCT GAGCTACCAG 1741 TCCAAGCTGA GCAAAGACCC CAACGAGAAG CGCGATCACA TGGTCCTGCT GGAGTTCGTG 1801 ACCGCCGCCG GGATCACTCT CGGCATGGAC GAGCTGTACA AGGAATTCCT GCCCCCCCTG 1861 GAGCGCCTGA CCCTGTAATC TAGAGGGCCC TATTctatag tgtcacctaa atgctagagc 1921 tcgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc 1981 cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga 2041 aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga 2101 cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat 2161 ggcttctgag gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag 2221 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag 2281 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt 2341 tccccgtcaa gctctaaatc ggggcatccc tttagggttc cgatttagtg ctttacggca 2401 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata 2461 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca 2521 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttggg 2581 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt 2641 ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagg caggcagaag 2701 tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc 2761 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct 2821 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg 2881 actaattttt tttatttatg cagaggccga ggccgcctct gcctctgagc tattccagaa 2941 gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctcccgg gagcttgtat 3001 atccattttc ggatctgatc aagagacagg atgaggatcg tttcgcatga ttgaacaaga 3061 tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc 3121 acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc 3181 ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcagg acgaggcagc 3241 gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac 3301 tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc 3361 tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac 3421 gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg 3481 tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct 3541 cgcgccagcc gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt 3601 cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg 3661 attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac 3721 ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg 3781 tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg 3841 agcgggactc tggggttcga aatgaccgac caagcgacgc ccaacctgcc atcacgagat 3901 ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc 3961 ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca ccccaacttg 4021 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa 4081 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat 4141 gtctgtatac cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct 4201 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt 4261 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc 4321 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg 4381 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg 4441 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca 4501 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac 4561 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac 4621 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg 4681 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac 4741 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc aatgctcacg ctgtaggtat 4801 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag 4861 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac 4921 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt 4981 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt 5041 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc 5101 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga 5161 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac 5221 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc 5281 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct 5341 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca 5401 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct 5461 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca 5521 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc 5581 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg 5641 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct 5701 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa 5761 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta 5821 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc 5881 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg 5941 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa 6001 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg 6061 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc 6121 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg 6181 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat 6241 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata 6301 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tc //