LOCUS       pcDNA3-AMPKsub(TA)-YPet-NES    6279 bp      DNA     circular   10/10/16
DEFINITION  
ACCESSION   
VERSION     
SOURCE      
  ORGANISM  
COMMENT     Serial Cloner Genbank Format
COMMENT     SerialCloner_Type=Degen. DNA
COMMENT     SerialCloner_Comments=
COMMENT     SerialCloner_Ends=0,0,,0,
FEATURES             Location/Qualifiers
     misc_feature    1009..1035
                     /label=AMPKsub_TA
                     /SerialCloner_Color=&h999999
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="direct strand translation" \ 
                     /SerialCloner_Desc=translation="MRRVAALVD" \ 
     gene            1063..1779
                     /label=YPET
                     /SerialCloner_Color=&hFFFF00
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     primer_bind     complement(4118..4138)
                     /label=M13-rev
                     /SerialCloner_Color=&h008B8B
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     primer_bind     863..882
                     /label=T7
                     /SerialCloner_Color=&h008B8B
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     primer_bind     complement(1832..1849)
                     /label=SP6
                     /SerialCloner_Color=&h008B8B
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     rep_origin      complement(4506..5134)
                     /label=ColE1 origin
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     misc_binding    complement(4144..4166)
                     /label=LacO
                     /SerialCloner_Color=&h6495ED
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     CDS             complement(5286..5945)
                     /label=AmpR
                     /SerialCloner_Color=&h8FBC8F
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     CDS             2987..3778
                     /label=Kan/neoR
                     /SerialCloner_Color=&h8FBC8F
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     Promoter        complement(6185..6213)
                     /label=Amp prom
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     Promoter        complement(4171..4200)
                     /label=lac
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     misc_feature    2768..2845
                     /label=SV40 ORI
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     terminator      1872..2099
                     /label=bGH PA
                     /SerialCloner_Color=&hFF8000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     misc_feature    232..819
                     /label=CMV prom
                     /SerialCloner_Color=&h800080
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     CDS             922..939
                     /label=6His
                     /SerialCloner_Color=&h008000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     misc_feature    1786..1812
                     /label=NES-Targeting
                     /SerialCloner_Color=&h800040
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg 
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 
      421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt 
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggAGACCCAA GCTTGCGGCC 
      901 GCCACCATGG TGCGGGGTTC TCATCATCAT CATCATCATG GTATGGCTAG CATGACTGGT 
      961 GGACAGCAAA TGGGTCGGGA TCTGTACGAC GATGACGATA AGGATCCcAT GAGGAGAGTG 
     1021 GCTGCCCTGG TGGATCTGGG CGGCACCGGC GGCAGCGAGC TCATGGTGAG CAAAGGCGAA 
     1081 GAGCTGTTCA CCGGCGTGGT GCCCATCCTG GTGGAGCTGG ACGGCGACGT GAACGGCCAC 
     1141 AAGTTCAGCG TGAGCGGCGA GGGCGAGGGC GACGCCACCT ACGGCAAGCT GACCCTGAAG 
     1201 CTGCTGTGCA CCACCGGCAA GCTGCCCGTG CCCTGGCCCA CCCTGGTGAC CACCCTGGGC 
     1261 TACGGCGTGC AGTGCTTCGC CCGGTACCCC GACCACATGA AGCAGCACGA CTTCTTCAAG 
     1321 AGCGCCATGC CCGAGGGCTA CGTGCAGGAG CGGACCATCT TCTTCAAGGA CGACGGCAAC 
     1381 TACAAGACCC GGGCCGAGGT GAAGTTCGAG GGCGACACCC TGGTGAACCG GATCGAGCTG 
     1441 AAGGGCATCG ACTTCAAGGA GGACAGCAAC ATCCTGGGCC ACAAGCTGGA GTACAACTAC 
     1501 AACAGCCACA ACGTGTACAT CACCGCCGAC AAGCAGAAGA ACGGCATCAA GGCCAACTTC 
     1561 AAGATCCGGC ACAACATCGA GGACGGCGGC GTGCAGCTGG CCGACCACTA CCAGCAGAAC 
     1621 ACCCCCATCG GCGACGGCCC CGTGCTGCTG CCCGATAACC ACTACCTGAG CTACCAGAGC 
     1681 GCCCTGTTCA AGGACCCCAA CGAGAAGCGG GACCACATGG TGCTGCTGGA GTTCCTGACC 
     1741 GCCGCCGGCA TCACCGAGGG TATGAATGAA TTGTACAAAG AATTCCTGCC CCCCCTGGAG 
     1801 CGCCTGACCC TGTAATCTAG AGGGCCCTAT Tctatagtgt cacctaaatg ctagagctcg 
     1861 ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt 
     1921 gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat 
     1981 tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag 
     2041 caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc 
     2101 ttctgaggcg gaaagaacca gctggggctc tagggggtat ccccacgcgc cctgtagcgg 
     2161 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc 
     2221 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc 
     2281 ccgtcaagct ctaaatcggg gcatcccttt agggttccga tttagtgctt tacggcacct 
     2341 cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac 
     2401 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac 
     2461 tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttggggat 
     2521 ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attaattctg 
     2581 tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccaggcag gcagaagtat 
     2641 gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc 
     2701 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac 
     2761 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact 
     2821 aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat tccagaagta 
     2881 gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc 
     2941 cattttcgga tctgatcaag agacaggatg aggatcgttt cgcatgattg aacaagatgg 
     3001 attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca 
     3061 acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt 
     3121 tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaggacg aggcagcgcg 
     3181 gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga 
     3241 agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca 
     3301 ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct 
     3361 tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac 
     3421 tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc 
     3481 gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc gacggcgagg atctcgtcgt 
     3541 gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt 
     3601 catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg 
     3661 tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat 
     3721 cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc 
     3781 gggactctgg ggttcgaaat gaccgaccaa gcgacgccca acctgccatc acgagatttc 
     3841 gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc 
     3901 tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc caacttgttt 
     3961 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca 
     4021 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc 
     4081 tgtataccgt cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg 
     4141 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa 
     4201 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct 
     4261 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga 
     4321 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc 
     4381 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa 
     4441 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt 
     4501 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa 
     4561 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt 
     4621 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg 
     4681 tccgcctttc tcccttcggg aagcgtggcg ctttctcaat gctcacgctg taggtatctc 
     4741 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc 
     4801 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta 
     4861 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct 
     4921 acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc 
     4981 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa 
     5041 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa 
     5101 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa 
     5161 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt 
     5221 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac 
     5281 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc 
     5341 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc 
     5401 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata 
     5461 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc 
     5521 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc 
     5581 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca 
     5641 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa 
     5701 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca 
     5761 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt 
     5821 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt 
     5881 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg 
     5941 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga 
     6001 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc 
     6061 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg 
     6121 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag 
     6181 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg 
     6241 gttccgcgca catttccccg aaaagtgcca cctgacgtc
//