LOCUS       pcDNA3-AMPKsub-YPet-Kras    6291 bp      DNA     circular   10/10/16
DEFINITION  
ACCESSION   
VERSION     
SOURCE      
  ORGANISM  
COMMENT     Serial Cloner Genbank Format
COMMENT     SerialCloner_Type=Degen. DNA
COMMENT     SerialCloner_Comments=
COMMENT     SerialCloner_Ends=0,0,,0,
FEATURES             Location/Qualifiers
     CDS             922..939
                     /label=6His
                     /SerialCloner_Color=&h008000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     misc_feature    1009..1035
                     /label=AMPKsub
                     /SerialCloner_Color=&h999999
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="direct strand translation"translation="MRRVATLVD" \ 
     gene            1063..1779
                     /label=YPET
                     /SerialCloner_Color=&hFFFF00
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     misc_feature    1786..1824
                     /label=Kras-Targeting
                     /SerialCloner_Color=&h800040
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="direct strand translation"translation="KKKKKSKTKCVIM" \ 
     primer_bind     complement(4130..4150)
                     /label=M13-rev
                     /SerialCloner_Color=&h008B8B
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     primer_bind     863..882
                     /label=T7
                     /SerialCloner_Color=&h008B8B
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     primer_bind     complement(1844..1861)
                     /label=SP6
                     /SerialCloner_Color=&h008B8B
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     rep_origin      complement(4518..5146)
                     /label=ColE1 origin
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     misc_binding    complement(4156..4178)
                     /label=LacO
                     /SerialCloner_Color=&h6495ED
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     CDS             complement(5298..5957)
                     /label=AmpR
                     /SerialCloner_Color=&h8FBC8F
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     CDS             2999..3790
                     /label=Kan/neoR
                     /SerialCloner_Color=&h8FBC8F
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     Promoter        complement(6197..6225)
                     /label=Amp prom
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     Promoter        complement(4183..4212)
                     /label=lac
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     misc_feature    2780..2857
                     /label=SV40 ORI
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     terminator      1884..2111
                     /label=bGH PA
                     /SerialCloner_Color=&hFF8000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     misc_feature    232..819
                     /label=CMV prom
                     /SerialCloner_Color=&h800080
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg 
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 
      421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt 
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggAGACCCAA GCTTGCGGCC 
      901 GCCACCATGG TGCGGGGTTC TCATCATCAT CATCATCATG GTATGGCTAG CATGACTGGT 
      961 GGACAGCAAA TGGGTCGGGA TCTGTACGAC GATGACGATA AGGATCCcAT GAGGAGAGTG 
     1021 GCTACCCTGG TGGATCTGGG CGGCACCGGC GGCAGCGAGC TCATGGTGAG CAAAGGCGAA 
     1081 GAGCTGTTCA CCGGCGTGGT GCCCATCCTG GTGGAGCTGG ACGGCGACGT GAACGGCCAC 
     1141 AAGTTCAGCG TGAGCGGCGA GGGCGAGGGC GACGCCACCT ACGGCAAGCT GACCCTGAAG 
     1201 CTGCTGTGCA CCACCGGCAA GCTGCCCGTG CCCTGGCCCA CCCTGGTGAC CACCCTGGGC 
     1261 TACGGCGTGC AGTGCTTCGC CCGGTACCCC GACCACATGA AGCAGCACGA CTTCTTCAAG 
     1321 AGCGCCATGC CCGAGGGCTA CGTGCAGGAG CGGACCATCT TCTTCAAGGA CGACGGCAAC 
     1381 TACAAGACCC GGGCCGAGGT GAAGTTCGAG GGCGACACCC TGGTGAACCG GATCGAGCTG 
     1441 AAGGGCATCG ACTTCAAGGA GGACAGCAAC ATCCTGGGCC ACAAGCTGGA GTACAACTAC 
     1501 AACAGCCACA ACGTGTACAT CACCGCCGAC AAGCAGAAGA ACGGCATCAA GGCCAACTTC 
     1561 AAGATCCGGC ACAACATCGA GGACGGCGGC GTGCAGCTGG CCGACCACTA CCAGCAGAAC 
     1621 ACCCCCATCG GCGACGGCCC CGTGCTGCTG CCCGATAACC ACTACCTGAG CTACCAGAGC 
     1681 GCCCTGTTCA AGGACCCCAA CGAGAAGCGG GACCACATGG TGCTGCTGGA GTTCCTGACC 
     1741 GCCGCCGGCA TCACCGAGGG TATGAATGAA TTGTACAAAG AATTCAAGAA GAAGAAGAAG 
     1801 AGCAAGACCA AGTGCGTGAT CATGTAATCT AGAGGGCCCT ATTctatagt gtcacctaaa 
     1861 tgctagagct cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg 
     1921 cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata 
     1981 aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt 
     2041 ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt 
     2101 gggctctatg gcttctgagg cggaaagaac cagctggggc tctagggggt atccccacgc 
     2161 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac 
     2221 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt 
     2281 cgccggcttt ccccgtcaag ctctaaatcg gggcatccct ttagggttcc gatttagtgc 
     2341 tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc 
     2401 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact 
     2461 cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg 
     2521 gattttgggg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc 
     2581 gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccaggc 
     2641 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc 
     2701 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt 
     2761 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc 
     2821 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct 
     2881 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcccggg 
     2941 agcttgtata tccattttcg gatctgatca agagacagga tgaggatcgt ttcgcatgat 
     3001 tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta 
     3061 tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca 
     3121 ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcagga 
     3181 cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga 
     3241 cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct 
     3301 cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg 
     3361 gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga 
     3421 gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca 
     3481 tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgcgcatgc ccgacggcga 
     3541 ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg 
     3601 cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc 
     3661 gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt 
     3721 gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga 
     3781 gttcttctga gcgggactct ggggttcgaa atgaccgacc aagcgacgcc caacctgcca 
     3841 tcacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc 
     3901 cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac 
     3961 cccaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc 
     4021 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta 
     4081 tcttatcatg tctgtatacc gtcgacctct agctagagct tggcgtaatc atggtcatag 
     4141 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc 
     4201 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc 
     4261 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa 
     4321 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg 
     4381 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg 
     4441 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag 
     4501 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac 
     4561 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga 
     4621 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt 
     4681 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca atgctcacgc 
     4741 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc 
     4801 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta 
     4861 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat 
     4921 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca 
     4981 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct 
     5041 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt 
     5101 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct 
     5161 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc 
     5221 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa 
     5281 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta 
     5341 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc 
     5401 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat 
     5461 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta 
     5521 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt 
     5581 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt 
     5641 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg 
     5701 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc 
     5761 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc 
     5821 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg 
     5881 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga 
     5941 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta 
     6001 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct 
     6061 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag 
     6121 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga 
     6181 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat 
     6241 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt c
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