LOCUS Exported 7509 bp ds-DNA circular SYN 09-MAY-2017 DEFINITION . ACCESSION . VERSION . KEYWORDS pcDNA3-mCherry-LKB1(S428A) SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 7509) AUTHORS . TITLE Direct Submission JOURNAL Exported Tuesday, May 9, 2017 from SnapGene 3.3.3 http://www.snapgene.com FEATURES Location/Qualifiers source 1..7509 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 1..588 /note="CMV prom" /note="SerialCloner_Protect=False SerialCloner_Color=&h800080 SerialCloner_Show=True SerialCloner_Arrow=True" /note="color: #800080; direction: RIGHT" primer_bind 632..651 /note="T7" /note="SerialCloner_Protect=False SerialCloner_Color=&h008B8B SerialCloner_Show=True SerialCloner_Arrow=True" /note="color: #008b8b; direction: RIGHT" CDS 691..708 /note="6His" /note="SerialCloner_Protect=False SerialCloner_Color=&h008000 SerialCloner_Show=True SerialCloner_Arrow=True" /note="color: #008000; direction: RIGHT" CDS 778..1485 /note="mCherry" /note="SerialCloner_Protect=False SerialCloner_Color=&hFF0000 SerialCloner_Show=True SerialCloner_Arrow=True" /note="color: #ff0000; direction: RIGHT" misc_feature 1498..2799 /note="LKB1_SA" /note="SerialCloner_Protect=False SerialCloner_Color=&h84A4C0 SerialCloner_Show=True SerialCloner_Arrow=True" /note="color: #84a4c0; direction: RIGHT" primer_bind complement(2831..2848) /note="SP6" /note="SerialCloner_Protect=False SerialCloner_Color=&h008B8B SerialCloner_Show=True SerialCloner_Arrow=True" /note="color: #008b8b; direction: LEFT" terminator 2871..3098 /note="bGH PA" /note="SerialCloner_Protect=False SerialCloner_Color=&hFF8000 SerialCloner_Show=True SerialCloner_Arrow=True" /note="color: #ff8000; direction: RIGHT" misc_feature 3767..3844 /note="SV40 ORI" /note="SerialCloner_Protect=False SerialCloner_Color=&h4682B4 SerialCloner_Show=True SerialCloner_Arrow=True" /note="color: #4682b4; direction: RIGHT" CDS 3986..4777 /note="Kan/neoR" /note="SerialCloner_Protect=False SerialCloner_Color=&h8FBC8F SerialCloner_Show=True SerialCloner_Arrow=True" /note="color: #8fbc8f; direction: RIGHT" primer_bind complement(5117..5137) /note="M13-rev" /note="SerialCloner_Protect=False SerialCloner_Color=&h008B8B SerialCloner_Show=True SerialCloner_Arrow=True" /note="color: #008b8b; direction: LEFT" misc_binding complement(5143..5165) /note="LacO" /note="SerialCloner_Protect=False SerialCloner_Color=&h6495ED SerialCloner_Show=True SerialCloner_Arrow=True" /note="color: #6495ed; direction: LEFT" promoter complement(5170..5199) /note="lac" /note="SerialCloner_Protect=False SerialCloner_Color=&h4682B4 SerialCloner_Show=True SerialCloner_Arrow=True" /note="color: #4682b4; direction: LEFT" rep_origin complement(5505..6133) /direction=LEFT /note="ColE1 origin" /note="SerialCloner_Protect=False SerialCloner_Color=&h4682B4 SerialCloner_Show=True SerialCloner_Arrow=True" /note="color: #4682b4" CDS complement(6285..6944) /note="AmpR" /note="SerialCloner_Protect=False SerialCloner_Color=&h8FBC8F SerialCloner_Show=True SerialCloner_Arrow=True" /note="color: #8fbc8f; direction: LEFT" promoter complement(7184..7212) /note="Amp prom" /note="SerialCloner_Protect=False SerialCloner_Color=&h4682B4 SerialCloner_Show=True SerialCloner_Arrow=True" /note="color: #4682b4; direction: LEFT" ORIGIN 1 gttgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata 61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc 121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag 181 ggactttcca ttgacgtcaa tgggtggact atttacggta aactgcccac ttggcagtac 241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg 301 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg 361 tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat 421 agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt 481 tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc 541 aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctctc tggctaacta 601 gagaacccac tgcttactgg cttatcgaaa ttaatacgac tcactatagg gagacccaag 661 cttgcggccg ccaccatggt gcggggttct catcatcatc atcatcatgg tatggctagc 721 atgactggtg gacagcaaat gggtcgggat ctgtacgacg atgacgataa ggatcccatg 781 gtgagcaagg gcgaggagga taacatggcc atcatcaagg agttcatgcg cttcaaggtg 841 cacatggagg gctccgtgaa cggccacgag ttcgagatcg agggcgaggg cgagggccgc 901 ccctacgagg gcacccagac cgccaagctg aaggtgacca agggtggccc cctgcccttc 961 gcctgggaca tcctgtcccc tcagttcatg tacggctcca aggcctacgt gaagcacccc 1021 gccgacatcc ccgactactt gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg 1081 atgaacttcg aggacggcgg cgtggtgacc gtgacccagg actcctccct gcaggacggc 1141 gagttcatct acaaggtgaa gctgcgcggc accaacttcc cctccgacgg ccccgtaatg 1201 cagaagaaga ccatgggctg ggaggcctcc tccgagcgga tgtaccccga ggacggcgcc 1261 ctgaagggcg agatcaagca gaggctgaag ctgaaggacg gcggccacta cgacgctgag 1321 gtcaagacca cctacaaggc caagaagccc gtgcagctgc ccggcgccta caacgtcaac 1381 atcaagttgg acatcacctc ccacaacgag gactacacca tcgtggaaca gtacgaacgc 1441 gccgagggcc gccactccac cggcggcatg gacgagctgt acaaggaatt ctccagcatg 1501 gaggtggtgg acccgcagca gctgggcatg ttcacggagg gcgagctgat gtcggtgggt 1561 atggacacgt tcatccaccg catcgactcc accgaggtca tctaccagcc gcgccgcaag 1621 cgggccaagc tcatcggcaa gtacctgatg ggggacctgc tgggggaagg ctcttacggc 1681 aaggtgaagg aggtgctgga ctcggagacg ctgtgcagga gggccgtcaa gatcctcaag 1741 aagaagaagt tgcgaaggat ccccaacggg gaggccaacg tgaagaagga aattcaacta 1801 ctgaggaggt tacggcacaa aaatgtcatc cagctggtgg atgtgttata caacgaagag 1861 aagcagaaaa tgtatatggt gatggagtac tgcgtgtgtg gcatgcagga aatgctggac 1921 agcgtgccgg agaagcgttt cccagtgtgc caggcccacg ggtacttctg tcagctgatt 1981 gacggcctgg agtacctgca tagccagggc attgtgcaca aggacatcaa gccggggaac 2041 ctgctgctca ccaccggtgg caccctcaaa atctccgacc tgggcgtggc cgaggcactg 2101 cacccgttcg cggcggacga cacctgccgg accagccagg gctccccggc tttccagccg 2161 cccgagattg ccaacggcct ggacaccttc tccggcttca aggtggacat ctggtcggct 2221 ggggtcaccc tctacaacat caccacgggt ctgtacccct tcgaagggga caacatctac 2281 aagttgtttg agaacatcgg gaaggggagc tacgccatcc cgggcgactg tggccccccg 2341 ctctctgacc tgctgaaagg gatgcttgag tacgaaccgg ccaagaggtt ctccatccgg 2401 cagatccggc agcacagctg gttccggaag aaacatcctc cggctgaagc accagtgccc 2461 atcccaccga gcccagacac caaggaccgg tggcgcagca tgactgtggt gccgtacttg 2521 gaggacctgc acggcgcgga cgaggacgag gacctcttcg acatcgagga tgacatcatc 2581 tacactcagg acttcacggt gcccggacag gtcccagaag aggaggccag tcacaatgga 2641 cagcgccggg gcctccccaa ggccgtgtgt atgaacggca cagaggcggc gcagctgagc 2701 accaaatcca gggcggaggg ccgggccccc aaccctgccc gcaaggcctg ctccgccagc 2761 agcaagatcc gccggctggc cgcctgcaag cagcagtgag gcgtcgagca tgcatctaga 2821 gggccctatt ctatagtgtc acctaaatgc tagagctcgc tgatcagcct cgactgtgcc 2881 ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg 2941 tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag 3001 gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga 3061 caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag 3121 ctggggctct agggggtatc cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt 3181 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc 3241 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg 3301 catcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta 3361 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt 3421 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat 3481 ctcggtctat tcttttgatt tataagggat tttggggatt tcggcctatt ggttaaaaaa 3541 tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg 3601 tgtggaaagt ccccaggctc cccaggcagg cagaagtatg caaagcatgc atctcaatta 3661 gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat 3721 gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac 3781 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga 3841 ggccgaggcc gcctctgcct ctgagctatt ccagaagtag tgaggaggct tttttggagg 3901 cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc attttcggat ctgatcaaga 3961 gacaggatga ggatcgtttc gcatgattga acaagatgga ttgcacgcag gttctccggc 4021 cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga 4081 tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct 4141 gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc tggccacgac 4201 gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct 4261 attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt 4321 atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt 4381 cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt 4441 cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag 4501 gctcaaggcg cgcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt 4561 gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg 4621 tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg 4681 cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg 4741 catcgccttc tatcgccttc ttgacgagtt cttctgagcg ggactctggg gttcgaaatg 4801 accgaccaag cgacgcccaa cctgccatca cgagatttcg attccaccgc cgccttctat 4861 gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg 4921 gatctcatgc tggagttctt cgcccacccc aacttgttta ttgcagctta taatggttac 4981 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt 5041 tgtggtttgt ccaaactcat caatgtatct tatcatgtct gtataccgtc gacctctagc 5101 tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca 5161 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg 5221 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg 5281 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc 5341 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta 5401 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag 5461 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg 5521 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg 5581 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg 5641 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga 5701 agcgtggcgc tttctcaatg ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc 5761 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt 5821 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact 5881 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg 5941 cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt 6001 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt 6061 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct 6121 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg 6181 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt 6241 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt 6301 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc 6361 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg 6421 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc 6481 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg 6541 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca 6601 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga 6661 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct 6721 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg 6781 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca 6841 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata 6901 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct 6961 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact 7021 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa 7081 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc 7141 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga 7201 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga 7261 aaagtgccac ctgacgtcga cggatcggga gatctcccga tcccctatgg tcgactctca 7321 gtacaatctg ctctgatgcc gcatagttaa gccagtatct gctccctgct tgtgtgttgg 7381 aggtcgctga gtagtgcgcg agcaaaattt aagctacaac aaggcaaggc ttgaccgaca 7441 attgcatgaa gaatctgctt agggttaggc gttttgcgct gcttcgcgat gtacgggcca 7501 gatatacgc //