LOCUS       pRARE_MAL_DEST        9756 bp    DNA     circular      8-JUL-2011
DEFINITION  Complementary copy of pRARE_MAL_DEST_REV.
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     VNTDATE|417459769|
COMMENT     VNTDBDATE|595168252|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pRARE_MAL_DEST|
COMMENT     VNTAUTHORNAME|Neil Olszewski|
COMMENT     VNTOAUTHORNAME|Demo User|
COMMENT     VNTREPLTYPE|Plasmid
COMMENT     VNTEXTCHREPL|Bacteria
FEATURES             Location/Qualifiers
     CDS             214..1170
                     /vntifkey="4"
                     /label=LacIq
     CDS             1538..2752
                     /vntifkey="4"
                     /label=MBP
     CDS             complement(2995..3801)
                     /vntifkey="4"
                     /label=KAN
     CDS             5415..6071
                     /vntifkey="4"
                     /label=CHL
     CDS             4236..4538
                     /vntifkey="4"
                     /label=CcdB
     tRNA            complement(8301..8377)
                     /vntifkey="59"
                     /label=ArgU
     tRNA            complement(6845..7117)
                     /vntifkey="59"
                     /label=ArgW
     tRNA            8624..8696
                     /vntifkey="59"
                     /label=ileX
     tRNA            complement(7625..7700)
                     /vntifkey="59"
                     /label=thrU
     tRNA            complement(7532..7616)
                     /vntifkey="59"
                     /label=TyrU
     primer          2626..2645
                     /vntifkey="27"
                     /label=MBP_F
BASE COUNT     2583 a      2335 c      2434 g      2404 t 
ORIGIN
        1 tcgagaaatt ccgacaccat cgaatggtgc aaaacctttc gcggtatggc atgatagcgc 
       61 ccggaagaga gtcaattcag ggtggtgaat gtgaaaccag taacgttata cgatgtcgca 
      121 gagtatgccg gtgtctctta tcagaccgtt tcccgcgtgg tgaaccaggc cagccacgtt 
      181 tctgcgaaaa cgcgggaaaa agtggaagcg gcgatggcgg agctgaatta cattcccaac 
      241 cgcgtggcac aacaactggc gggcaaacag tcgttgctga ttggcgttgc cacctccagt 
      301 ctggccctgc acgcgccgtc gcaaattgtc gcggcgatta aatctcgcgc cgatcaactg 
      361 ggtgccagcg tggtggtgtc gatggtagaa cgaagcggcg tcgaagcctg taaagcggcg 
      421 gtgcacaatc ttctcgcgca acgcgtcagt gggctgatca ttaactatcc gctggatgac 
      481 caggatgcca ttgctgtgga agctgcctgc actaatgttc cggcgttatt tcttgatgtc 
      541 tctgaccaga cacccatcaa cagtattatt ttctcccatg aagacggtac gcgactgggc 
      601 gtggagcatc tggtcgcatt gggtcaccag caaatcgcgc tgttagcggg cccattaagt 
      661 tctgtctcgg cgcgtctgcg tctggctggc tggcataaat atctcactcg caatcaaatt 
      721 cagccgatag cggaacggga aggcgactgg agtgccatgt ccggttttca acaaaccatg 
      781 caaatgctga atgagggcat cgttcccact gcgatgctgg ttgccaacga tcagatggcg 
      841 ctgggcgcaa tgcgcgccat taccgagtcc gggctgcgcg ttggtgcgga tatctcggta 
      901 gtgggatacg acgataccga agacagctca tgttatatcc cgccgttaac caccatcaaa 
      961 caggattttc gcctgctggg gcaaaccagc gtggaccgct tgctgcaact ctctcagggc 
     1021 caggcggtga agggcaatca gctgttgccc gtctcactgg tgaaaagaaa aaccaccctg 
     1081 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca 
     1141 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg taagttagct 
     1201 cactcattag gcacaattct catgtttgac agcttatcat cgactgcacg gtgcaccaat 
     1261 gcttctggcg tcaggcagcc atcggaagct gtggtatggc tgtgcaggtc gtaaatcact 
     1321 gcataattcg tgtcgctcaa ggcgcactcc cgttctggat aatgtttttt gcgccgacat 
     1381 cataacggtt ctggcaaata ttctgaaatg agctgttgac aattaatcat cggctcgtat 
     1441 aatgtgtgga attgtgagcg gataacaatt tcacacagga aacagccagt ccgtttaggt 
     1501 gttttcacga gcacttcacc aacaaggacc atagattatg aaaactgaag aaggtaaact 
     1561 ggtaatctgg attaacggcg ataaaggcta taacggtctc gctgaagtcg gtaagaaatt 
     1621 cgagaaagat accggaatta aagtcaccgt tgagcatccg gataaactgg aagagaaatt 
     1681 cccacaggtt gcggcaactg gcgatggccc tgacattatc ttctgggcac acgaccgctt 
     1741 tggtggctac gctcaatctg gcctgttggc tgaaatcacc ccggacaaag cgttccagga 
     1801 caagctgtat ccgtttacct gggatgccgt acgttacaac ggcaagctga ttgcttaccc 
     1861 gatcgctgtt gaagcgttat cgctgattta taacaaagat ctgctgccga acccgccaaa 
     1921 aacctgggaa gagatcccgg cgctggataa agaactgaaa gcgaaaggta agagcgcgct 
     1981 gatgttcaac ctgcaagaac cgtacttcac ctggccgctg attgctgctg acgggggtta 
     2041 tgcgttcaag tatgaaaacg gcaagtacga cattaaagac gtgggcgtgg ataacgctgg 
     2101 cgcgaaagcg ggtctgacct tcctggttga cctgattaaa aacaaacaca tgaatgcaga 
     2161 caccgattac tccatcgcag aagctgcctt taataaaggc gaaacagcga tgaccatcaa 
     2221 cggcccgtgg gcatggtcca acatcgacac cagcaaagtg aattatggtg taacggtact 
     2281 gccgaccttc aagggtcaac catccaaacc gttcgttggc gtgctgagcg caggtattaa 
     2341 cgccgccagt ccgaacaaag agctggcaaa agagttcctc gaaaactatc tgctgactga 
     2401 tgaaggtctg gaagcggtta ataaagacaa accgctgggt gccgtagcgc tgaagtctta 
     2461 cgaggaagag ttggcgaaag atccacgtat tgccgccacc atggaaaacg cccagaaagg 
     2521 tgaaatcatg ccgaacatcc cgcagatgtc cgctttctgg tatgccgtgc gtactgcggt 
     2581 gatcaacgcc gccagcggtc gtcagactgt cgatgaagcc ctgaaagacg cgcagactaa 
     2641 ttcgagctcg aacaacaaca acaataacaa taacaacaac ctcgggatcg agggaaggat 
     2701 ttcagaattc ctgaatcaaa caagtttgta caaaaaagct gaacgagaaa cgtaaaatga 
     2761 tataaatatc aatatattaa attagatttt gcataaaaaa cagactacat aatactgtaa 
     2821 aacacaacat atccagtcac tatggcggcc gcattaggca ccccaggctt tacactttat 
     2881 gcttccggct cgtataatgt gtggattttg agttaggatc catgagcttg cgccgtcccg 
     2941 tcaagtcagc gtaatgctct gccagtgtta caaccaatta accaattctg attagaaaaa 
     3001 ctcatcgagc atcaaatgaa actgcaattt attcatatca ggattatcaa taccatattt 
     3061 ttgaaaaagc cgtttctgta atgaaggaga aaactcaccg aggcagttcc ataggatggc 
     3121 aagatcctgg tatcggtctg cgattccgac tcgtccaaca tcaatacaac ctattaattt 
     3181 cccctcgtca aaaataaggt tatcaagtga gaaatcacca tgagtgacga ctgaatccgg 
     3241 tgagaatggc aaaagtttat gcatttcttt ccagacttgt tcaacaggcc agccattacg 
     3301 ctcgtcatca aaatcactcg catcaaccaa accgttattc attcgtgatt gcgcctgagc 
     3361 gagacgaaat acgcgatcgc tgttaaaagg acaattacaa acaggaatcg aatgcaaccg 
     3421 gcgcaggaac actgccagcg catcaacaat attttcacct gaatcaggat attcttctaa 
     3481 tacctggaat gctgtttttc cggggatcgc agtggtgagt aaccatgcat catcaggagt 
     3541 acggataaaa tgcttgatgg tcggaagagg cataaattcc gtcagccagt ttagtctgac 
     3601 catctcatct gtaacatcat tggcaacgct acctttgcca tgtttcagaa acaactctgg 
     3661 cgcatcgggc ttcccataca agcgatagat tgtcgcacct gattgcccga cattatcgcg 
     3721 agcccattta tacccatata aatcagcatc catgttggaa tttaatcgcg gcctcgacgt 
     3781 ttcccgttga atatggctca taacacccct tgtattactg tttatgtaag cagacagttt 
     3841 tattgttcat gatgatatat ttttatcttg tgcaatgtaa catcagagat tttgagacac 
     3901 ggatccggct tactaaaagc cagataacag tatgcgtatt tgcgcgctga tttttgcggt 
     3961 ataagaatat atactgatat gtatacccga agtatgtcaa aaagaggtat gctatgaagc 
     4021 agcgtattac agtgacagtt gacagcgaca gctatcagtt gctcaaggca tatatgatgt 
     4081 caatatctcc ggtctggtaa gcacaaccat gcagaatgaa gcccgtcgtc tgcgtgccga 
     4141 acgctggaaa gcggaaaatc aggaagggat ggctgaggtc gcccggttta ttgaaatgaa 
     4201 cggctctttt gctgacgaga acaggggctg gtgaaatgca gtttaaggtt tacacctata 
     4261 aaagagagag ccgttatcgt ctgtttgtgg atgtacagag tgatattatt gacacgcccg 
     4321 ggcgacggat ggtgatcccc ctggccagtg cacgtctgct gtcagataaa gtcccccgtg 
     4381 aactttaccc ggtggtgcat atcggggatg aaagctggcg catgatgacc accgatatgg 
     4441 ccagtgtgcc ggtctccgtt atcggggaag aagtggctga tctcagccac cgcgaaaatg 
     4501 acatcaaaaa cgccattaac ctgatgttct ggggaatata aatgtcaggc tcccttatac 
     4561 acagccagtc tgcaggtcga ccatagtgac tggatatgtt gtgttttaca gtattatgta 
     4621 gtctgttttt tatgcaaaat ctaatttaat atattgatat ttatatcatt ttacgtttct 
     4681 cgttcagctt tcttgtacaa agtggtttga ttcgaaagct tggcactggc cgtcgtttta 
     4741 caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc 
     4801 cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg 
     4861 cgcagcctga atggcgaatg gcagcttggc tgttttggcg gatgagataa gattttcagc 
     4921 ctgatacaga ttaaatcaga acgcagaagc ggtctgataa aacagaattt gcctggcggc 
     4981 agtagcgcgg tggtcccacc tgaccccatg ccgaactcag aagtgaaacg ccgtagcgcc 
     5041 gatggtagtg tggggtctcc cctcgaggct tttgccgtta cgcaccaccc cgtcagtagc 
     5101 tgaacaggag ggacagctga tagaaacaga agccactgga gcacctcaaa aacaccatca 
     5161 tacactaaat cagtaagttg gcagcatcac ccgacgcact ttgcgccgaa taaatacctg 
     5221 tgacggaaga tcacttcgca gaataaataa atcctggtgt ccctgttgct accgggaagc 
     5281 cctgggccga cttttggcga aaatgagacg ttgatcggca cgtaagaggt tccaactttc 
     5341 accataatga aataagatca ctaccgggcg tattttttga gttatcgaga ttttcaggag 
     5401 ctaaggaagc taaaatggag aaaaaaatca ctggatatac caccgttgat atatcccaat 
     5461 ggcatcgtaa agaacatttt gaggcatttc agtcagttgc tcaatgtacc tataaccaga 
     5521 ccgttcagct ggatattacg gcctttttaa agaccgtaaa gaaaaataag cacaagtttt 
     5581 atccggcctt tattcacatt cttgcccgcc tgatgaatgc tcatccggaa ttccgtatgg 
     5641 caatgaaaga cggtgagctg gtgatatggg atagtgttca cccttgttac accgttttcc 
     5701 atgagcaaac tgaaacgttt tcatcgctct ggagtgaata ccacgacgat ttccggcagt 
     5761 ttctacacat atattcgcaa gatgtggcgt gttacggtga aaacctggcc tatttcccta 
     5821 aagggtttat tgagaatatg tttttcgtct cagccaatcc ctgggtgagt ttcaccagtt 
     5881 ttgatttaaa cgtggccaat atggacaact tcttcgcccc cgttttcacc atgggcaaat 
     5941 attatacgca aggcgacaag gtgctgatgc cgctggcgat tcaggttcat catgccgttt 
     6001 gtgatggctt ccatgtcggc agaatgctta atgaattaca acagtactgc gatgagtggc 
     6061 agggcggggc gtaatttttt taaggcagtt attggtgccc ttaaacgcct ggttgctacg 
     6121 cctgaataag tgataataag cggatgaatg gcagaaattc gaaagcagat tcgacccggt 
     6181 cgtcggttca gggcagggtc gttaaatagc cgcttatgtc tattgctggt ttaccggttt 
     6241 attgactacc ggaagcagtg tgaccgtgtg cttctcaaat gcctgaggtc tgtttatcga 
     6301 attaattgca gatataaaaa aaccaaccgt aagggttggt tttttcttgg gatttttggt 
     6361 cggcacgaga ggatttgaac ctccgacccc cgacacccca tgacggtgcg ctaccaggct 
     6421 gcgctacgtg ccgactcgtg gctgctaata ctaccgtttt ccacaccgat tgcaagtaag 
     6481 atatttcgct aactgattta taattaatca gttagcgata aaacgcttct cgtacaacgc 
     6541 tttctggtga atggtgcggg aggcgagact tgaactcgca caccttgcgg cgccagaacc 
     6601 taaatctggt gcgtctacca atttcgccac tcccgcaaaa aaagatggtg gctacgacgg 
     6661 gattcgaacc tgtgacccca tcattatgag tgatgtgctc taaccaactg agctacgtag 
     6721 ccatcttttt tttcgcgata ccttatcggc gttgcggggc gcattatgcg tatagagcct 
     6781 tgcagcgtca acctcttttt caaggaaaat tgctcgaaag tgactgtttg gttaggttgc 
     6841 gaacagcgaa ccatgacgaa ctgtaaatct acggaatgct tgatattcag gggatttttg 
     6901 cggactggta cggatgggag cgaactgata aatggtgtcc cctgcaggaa tcgaacctgc 
     6961 aattagccct taggaggggc tcgttatatc catttaacta agaggacaat gcggcatgag 
     7021 tatacccgct aatggagtgc ggggtaagta cgttgccgct cgattgctta aaccctcgcc 
     7081 atttatgccg ggtttttata atttttctta atgttttccg cacgttctgc tttttggacg 
     7141 tcatcgattg tccctctaag acacggataa atcggtgata tcaccacatc aaccaggcaa 
     7201 catgcccgac ttgttgaatg caataaacag aaggaaaaaa cagggaggag aaaagaagtg 
     7261 gtgctgatag gcagattcga actgccgacc tcacccttac caagggtgcg ctctaccaac 
     7321 tgagctatat cagcacatct tggagcgggc agcgggaatc gaacccgcat catcagcttg 
     7381 gaaggctgag gtaatagcca ttatacgatg cccgcatcct ggaactcggc tacctgattt 
     7441 tcattctgca ctgaatatcg agagaagctc tctttattcg agccggtaag cgaacttatc 
     7501 gtctcgggct acgccatcgc gtggccgaaa ttggtggtgg gggaaggatt cgaaccttcg 
     7561 aagtctgtga cggcagattt acagtctgct ccctttggcc gctcgggaac cccaccggac 
     7621 ttgatggtgc cgactaccgg aatcgaactg gtgacctact gattacaagt cagttgctct 
     7681 acctactgag ccaagtcggc atcaagtagc gcgcactcta tggagacatg cgagttcatg 
     7741 caactaaaaa attgcataat ttgttttatt ggtcacattt tatgcgacac gatgaagaaa 
     7801 cagcctaggt acctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg 
     7861 gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg 
     7921 gcgtagagga tccgtcacat accaaggcgg ctaagcgagc agatggaaca tcaacgcctg 
     7981 cggtcaggaa gatgcgcatc gacagcaaga catcatggct atcagcttgt cgtcgtgcag 
     8041 gaattgaaga tttccgtttc catgacctca gacacacctg ggcaagctgg ctgattcagt 
     8101 caggcgtccc attatcagtg cttcaggaaa tgggcggatg ggagtccata gaaatggttc 
     8161 gtaggtatgc tcaccttgcg cctaatcatt tgacagagca tgcgaggaaa atagacgaca 
     8221 tttttggtga taatgtccca aatatgtccc actctgaaat tatggaggat ataaagaagg 
     8281 cgtaactgat tgaattgtaa tggcgcgccc tgcaggattc gaacctgcgg cccacgactt 
     8341 agaaggtcgt tgctctatcc aactgagcta agggcgcgtt gataccgcaa tgcggtgtaa 
     8401 tcgcgtgaat tatacggtca acccttgctg agtcaatggc ttttgatctg gttgctgaac 
     8461 aagtgaacga ccgcgtctga ttttctgatt tatttcgcta tagcggcaaa caaacgcaca 
     8521 ccgctgcgcg tctgaatcaa gaaaacccgt attttcatgt atcaaagtga cctgcagcca 
     8581 agcttggatt gcgacacgga gttactttat aatccaatcg attggcccct tagctcagtg 
     8641 gttagagcag gcgactcata atcgcttggt cgctgcttca agtccagcag gggccaccag 
     8701 cggccgcaaa ggctgacgag aaatcgtcag cctttttaag ctttaagccg aattccagca 
     8761 cactggcggc cgttactagt ggatccgagc tcggtaccaa gcttatcgat gataagctgt 
     8821 caaacatgag aattacaact tatatcgtat ggggctgact tcaggtgcta catttgaaga 
     8881 gataaattgc actgaaatct agaaatattt tatctgatta ataagatgat cttcttgaga 
     8941 tcgttttggt ctgcgcgtaa tctcttgctc tgaaaacgaa aaaaccgcct tgcagggcgg 
     9001 tttttcgaag gttctctgag ctaccaactc ttgaaccgag gtaactggct tggaggagcg 
     9061 cagtcaccaa aacttgtcct ttcagtttag ccttaaccgg cgcatgactt caagactaac 
     9121 tcctctaaat caattaccag tggctgctgc cagtggtgct tttgcatgtc tttccgggtt 
     9181 ggactcaaga cgatagttac cggataaggc gcagcggtcg gactgaacgg ggggttcgtg 
     9241 catacagtcc agcttggagc gaactgccta cccggaactg agtgtcaggc gtggaatgag 
     9301 acaaacgcgg ccataacagc ggaatgacac cggtaaaccg aaaggcagga acaggagagc 
     9361 gcacgaggga gccgccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc 
     9421 accactgatt tgagcgtcag atttcgtgat gcttgtcagg ggggcggagc ctatggaaaa 
     9481 acggctttgc cgcggccctc tcacttccct gttaagtatc ttcctggcat cttccaggaa 
     9541 atctccgccc cgttcgtaag ccatttccgc tcgccgcagt cgaacgaccg agcgtagcga 
     9601 gtcagtgagc gaggaagcgg aatatatcct gtatcacata ttctgctgac gcaccggtgc 
     9661 agcctttttt ctcctgccac atgaagcact tcactgacac cctcatcagt gccaacatag 
     9721 taagccagta tacactccgc tagcgcgatg tccggc 
//
