LOCUS       pMyBADNT        5472 bp    DNA     circular SYN 17-NOV-2016
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    pMyBADNT.
SOURCE      synthetic DNA construct.
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5472)
  AUTHORS   Kate Beckham
  TITLE     Direct Submission
  JOURNAL   Exported 12 Oct 2016 from SnapGene Viewer 3.0.2
            http://www.snapgene.com
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|-11506407|
COMMENT     VNTDBDATE|-11506407|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pMyBADNT|
COMMENT     VNTAUTHORNAME|Demo User|
COMMENT     VNTOAUTHORNAME|UNKNOWN|
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pMyBADNT" 0 5472 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 1 0 0 "MAIN" 1 1 1 1 0 0 1 1 1 0 10 5 4294967295 150 0 
COMMENT        1 0 (CHomObj 1 0 0 3 100) (CWordArray) (CWordArray) 
COMMENT        (CStringList "BamHI" "ClaI" "EcoRI" "EcoRV" "HindIII" "KpnI" "NcoI" 
COMMENT         "SalI") (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") 
COMMENT        (CObList 
COMMENT         #0=(COligo "AP-328 (seq pMyNT)" "CGCAGTTGTTCTCGCATACC" 
COMMENT             "sequencing primer for pMyNT, binds 3' acetamidase promoter" 0 
COMMENT             (CStringList) 0) 
COMMENT         #1=(COligo "AP-329 (seq pMyNT)" "CTACATCGCCAGCGCCGACG" "" 0 
COMMENT             (CStringList) 0) 
COMMENT         #2=(COligo "AP-330 (seq pMyNT)" "CCCTGATTCTGTGGATAACCG" 
COMMENT             "sequencing primer mycobacterial origin of replication " 0 
COMMENT             (CStringList) 0) 
COMMENT         #3=(COligo "AP-338 (seq pMyNT)" "GTTGAGCGGTGGCGCGTCG" 
COMMENT             "sequencing primer for pMy" 0 (CStringList) 0) 
COMMENT         #4=(COligo "AP-340 (seq pMyNT)" "CGAGCGCAACGAGACCCTCG" "" 0 
COMMENT             (CStringList) 0) 
COMMENT         #5=(COligo "AP-380 (seq pMyNT)" "AACTTACGGAGCTGGTGGGCTC" "" 0 
COMMENT             (CStringList) 0) 
COMMENT         #6=(COligo "AP-386 (seq pMyBAD)" "CCTGACGCTTTTTATCGCAACTC" 
COMMENT             "sequencing primer pMyBAD" 0 (CStringList) 0) 
COMMENT         #7=(COligo "AP-386rc (seq pMyBAD)" "GAGTTGCGATAAAAAGCGTCAGG" 
COMMENT             "binds arabinose promoter, reads into araC gene" 0 (CStringList) 0) 
COMMENT         #8=(COligo "AP-387 (seq pMy)" "TTGATGACGAGCGTAATGGC" 
COMMENT             "binds KanR in pMy (reverse)" 0 (CStringList) 0) 
COMMENT         #9=(COligo "AP-388 (seq pMyBAD)" "GACGACCGTAGTGATGAATC" "binds araC" 0 
COMMENT             (CStringList) 0) 
COMMENT         #10=(COligo "pMyNT-FWD (MWG)" "GGAGTCCACCATGAAGCACC" "" 0 (CStringList) 
COMMENT              0) 
COMMENT         #11=(COligo "pMyNT-REV (MWG)" "TGGATCTCTCCGGCTTCAC" "" 0 (CStringList) 
COMMENT              0)) 1 "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 1 "MAIN" 0 0 30 
COMMENT        0 (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 0 4294967295 1 5472 0 0 0 0 0 0 0 0) (CStringList) 
COMMENT       (CStringList) (CObList) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList 
COMMENT        #12=(CRSite (CStringList) "BamHI" "ggatcc" 2 0 2 1768 0 1922 0 0 "") 
COMMENT        #13=(CRSite (CStringList) "ClaI" "atcgat" 3 0 1 1953 0 0 "") 
COMMENT        #14=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 2 1928 0 2998 0 0 "") 
COMMENT        #15=(CRSite (CStringList) "EcoRV" "gatatc" 4 0 2 915 0 1936 0 0 "") 
COMMENT        #16=(CRSite (CStringList) "HindIII" "aagctt" 2 0 1 1946 0 0 "") 
COMMENT        #17=(CRSite (CStringList) "KpnI" "ggtacc" 6 0 2 606 0 1920 0 0 "") 
COMMENT        #18=(CRSite (CStringList) "NcoI" "ccatgg" 2 0 1 1903 0 0 "") 
COMMENT        #19=(CRSite (CStringList) "SalI" "gtcgac" 2 0 2 1958 0 2350 0 0 "")) 
COMMENT       (CObList 
COMMENT        #20=(CFSignal (CObList) "AraC" 4 0 1 624 1502 0 (CStringList) 
COMMENT             (CStringList "/codon_start=1" "/gene=\"AraC other\"") 1 1 1 1 
COMMENT             "complement(624..1502)") 
COMMENT        #21=(CFSignal (CObList) "araO2 reg" 21 0 0 1532 1547 0 (CStringList) 
COMMENT             (CStringList "/gene=\"araO2 reg\"") 1 1 1 1 "1532..1547") 
COMMENT        #22=(CFSignal (CObList) "CAP_BS other" 21 0 0 1731 1744 0 (CStringList) 
COMMENT             (CStringList "/gene=\"CAP_BS other\"") 1 1 1 1 "1731..1744") 
COMMENT        #23=(CFSignal (CObList) "AraI1I2 other" 21 0 0 1741 1779 0 (CStringList) 
COMMENT             (CStringList "/gene=\"AraI1I2 other\"") 1 1 1 1 "1741..1779") 
COMMENT        #24=(CFSignal (CObList) "ARA prom" 21 0 0 1776 1804 0 (CStringList) 
COMMENT             (CStringList "/gene=\"ARA prom\"") 1 1 1 1 "1776..1804") 
COMMENT        #25=(CFSignal (CObList) "Terminator_1" 43 0 0 1997 2043 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "1997..2043") 
COMMENT        #26=(CFSignal (CObList) "HygR" 4 0 1 2079 3077 0 (CStringList) 
COMMENT             (CStringList "/codon_start=1") 1 1 1 1 "complement(2079..3077)") 
COMMENT        #27=(CFSignal (CObList) "Rep_Origin_1" 33 0 0 3348 3748 0 (CStringList) 
COMMENT             (CStringList "/direction=RIGHT") 1 1 1 1 "3348..3748") 
COMMENT        #28=(CFSignal (CObList) "Rep_Origin_2" 33 0 0 4894 5410 0 (CStringList) 
COMMENT             (CStringList "/direction=RIGHT") 1 1 1 1 "4894..5410") 
COMMENT        #29=(CFSignal (CObList) "sequence-verified" 21 0 0 685 704 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "") 
COMMENT        #30=(CFSignal (CObList) "araBAD" 30 0 0 685 2296 0 (CStringList) 
COMMENT             (CStringList "/gene=\"araBAD\"") 1 1 1 1 "1529..1813")) 
COMMENT       (CObList 
COMMENT        #31=(CMSite "AP-328 (seq pMyNT)" "CGCAGTTGTTCTCGCATACC" 
COMMENT             "sequencing primer for pMyNT, binds 3' acetamidase promoter" 0) 
COMMENT        #32=(CMSite "AP-329 (seq pMyNT)" "CTACATCGCCAGCGCCGACG" "" 0) 
COMMENT        #33=(CMSite "AP-330 (seq pMyNT)" "CCCTGATTCTGTGGATAACCG" 
COMMENT             "sequencing primer mycobacterial origin of replication " 1 4082 0 
COMMENT             100 100 100) 
COMMENT        #34=(CMSite "AP-338 (seq pMyNT)" "GTTGAGCGGTGGCGCGTCG" 
COMMENT             "sequencing primer for pMy" 0) 
COMMENT        #35=(CMSite "AP-340 (seq pMyNT)" "CGAGCGCAACGAGACCCTCG" "" 0) 
COMMENT        #36=(CMSite "AP-380 (seq pMyNT)" "AACTTACGGAGCTGGTGGGCTC" "" 1 4179 1 
COMMENT             100 100 100) 
COMMENT        #37=(CMSite "AP-386 (seq pMyBAD)" "CCTGACGCTTTTTATCGCAACTC" 
COMMENT             "sequencing primer pMyBAD" 1 1775 0 100 100 100) 
COMMENT        #38=(CMSite "AP-386rc (seq pMyBAD)" "GAGTTGCGATAAAAAGCGTCAGG" 
COMMENT             "binds arabinose promoter, reads into araC gene" 1 1775 1 100 100 
COMMENT             100) 
COMMENT        #39=(CMSite "AP-387 (seq pMy)" "TTGATGACGAGCGTAATGGC" 
COMMENT             "binds KanR in pMy (reverse)" 0) 
COMMENT        #40=(CMSite "AP-388 (seq pMyBAD)" "GACGACCGTAGTGATGAATC" "binds araC" 1 
COMMENT             1250 0 100 100 100) 
COMMENT        #41=(CMSite "pMyNT-FWD (MWG)" "GGAGTCCACCATGAAGCACC" "" 0) 
COMMENT        #42=(CMSite "pMyNT-REV (MWG)" "TGGATCTCTCCGGCTTCAC" "" 1 2140 1 100 100 
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COMMENT                          "DNA 'pMyBADNT'" 1) 
COMMENT                     #47=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "synthetic circular DNA." 1) 
COMMENT                     #48=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Currently local object. Original author: UNKNOWN" 1) 
COMMENT                     #49=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Created: 11/17/16 11:42" 1) 
COMMENT                     #50=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Last Modified: 11/17/16 11:42" 1) 
COMMENT                     #51=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 5472 bp" 1) 
COMMENT                     #52=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "storage type: Basic" 1) 
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COMMENT                          "form: Circular" 1))) "General Description") 
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COMMENT                          "Keywords: pMyBADNT" 1) 
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COMMENT                          "Original Source Database: GenBank" 1) 
COMMENT                     #57=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Division: SYN" 1) 
COMMENT                     #58=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Modification Date in the Original DB: 12-OCT-2016" 1) 
COMMENT                     #59=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Original Accession Number(s): <a href=\"ncbilink:dnarna/.\">.</a>" 
COMMENT                          1) 
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COMMENT                          "Version in the Original DB: ." 1) 
COMMENT                     #61=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Sequence Source: synthetic DNA construct" 1) 
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COMMENT                          "Organism(s): <a href=\"ncbilink:taxonomy/synthetic+DNA+construct\">synthetic DNA construct(synthetic DNA construct)</a>" 
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COMMENT                          "Associated Genes:  AraC other;  araBAD;  araO2 reg;  CAP_BS other;  AraI1I2 other;  ARA prom" 
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COMMENT                          "REFERENCE   1  (bases 1 to 5472)" 1) 
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COMMENT                          "  AUTHORS   Kate Beckham" 1) 
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COMMENT                          "  TITLE     Direct Submission" 1) 
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COMMENT                          "  JOURNAL   Exported 12 Oct 2016 from SnapGene Viewer 3.0.2" 
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COMMENT                          "            http://www.snapgene.com" 1))) 
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COMMENT                          "CDS (2 total)") 
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COMMENT                                           "araBAD promoter promoter of the L-arabinose operon of E. coli; the  araC regulatory gene is transcribed in the opposite direction (Guzman et al., 1995)" 
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COMMENT                                           "Start: 1997  End: 2043" 1) 
COMMENT                                     #146=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "rnnB T1 terminator" 1) 
COMMENT                                     #147=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #148=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   1997..2043" 1))) "Terminator_1"))) 
COMMENT                           "Terminator (1 total)"))) "Feature Map") 
COMMENT              #149=(CFolderPar 
COMMENT                    (CGroupPar (CParagraph 12 (6 0) 1 1 0 0 178) 
COMMENT                     (CObjectList 
COMMENT                      #150=(CFolderPar 
COMMENT                            (CGroupPar (CParagraph 98 (0 0) 1 1 1 0 178) 
COMMENT                             (CObjectList 
COMMENT                              #151=(CFolderPar 
COMMENT                                    (CGroupPar (CParagraph 1 (0 0) 1 2 2 0 178) 
COMMENT                                     (CObjectList 
COMMENT                                      #152=(CLinePar 
COMMENT                                            (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                            "Start: 1     End: 5472" 1) 
COMMENT                                      #153=(CLinePar 
COMMENT                                            (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                            "Original Location Description:" 1) 
COMMENT                                      #154=(CLinePar 
COMMENT                                            (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                            "   1..5472" 1) 
COMMENT                                      #155=(CLinePar 
COMMENT                                            (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                            "Qualifiers:" 1) 
COMMENT                                      #156=(CLinePar 
COMMENT                                            (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                            "   /organism=\"recombinant plasmid\"" 
COMMENT                                            1) 
COMMENT                                      #157=(CLinePar 
COMMENT                                            (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                            "   /mol_type=\"other DNA\"" 1))) 
COMMENT                                    "Source_1"))) "Source (1 total)"))) 
COMMENT                    "Imported Features Not Shown on Map") 
COMMENT              #158=(CFolderPar 
COMMENT                    (CGroupPar (CParagraph 13 (0 0) 1 1 0 0 178) 
COMMENT                     (CObjectList 
COMMENT                      #159=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 60869008 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #160=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #12# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #161=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #12# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 1768 " 1) 
COMMENT                                      #162=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #12# 2) 1 2 2 0 191) 
COMMENT                                            " N2: 1922 " 1))))) "BamHI: 2 sites") 
COMMENT                            1 5 "GGATCC" "CCTAGG") 
COMMENT                      #163=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 60877392 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #164=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #13# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #165=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #13# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 1953 " 1))))) "ClaI: 1 site") 
COMMENT                            2 4 "ATCGAT" "TAGCTA") 
COMMENT                      #166=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 60877328 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #167=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #14# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #168=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #14# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 1928 " 1) 
COMMENT                                      #169=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #14# 2) 1 2 2 0 191) 
COMMENT                                            " N2: 2998 " 1))))) "EcoRI: 2 sites") 
COMMENT                            1 5 "GAATTC" "CTTAAG") 
COMMENT                      #170=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 60866608 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #171=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #15# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #172=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #15# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 915 " 1) 
COMMENT                                      #173=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #15# 2) 1 2 2 0 191) 
COMMENT                                            " N2: 1936 " 1))))) "EcoRV: 2 sites") 
COMMENT                            3 3 "GATATC" "CTATAG") 
COMMENT                      #174=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 60867760 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #175=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #16# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #176=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #16# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 1946 " 1))))) 
COMMENT                             "HindIII: 1 site") 1 5 "AAGCTT" "TTCGAA") 
COMMENT                      #177=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 60867504 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #178=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #17# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #179=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #17# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 606 " 1) 
COMMENT                                      #180=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #17# 2) 1 2 2 0 191) 
COMMENT                                            " N2: 1920 " 1))))) "KpnI: 2 sites") 
COMMENT                            5 1 "GGTACC" "CCATGG") 
COMMENT                      #181=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 60867536 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #182=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #18# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #183=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #18# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 1903 " 1))))) "NcoI: 1 site") 
COMMENT                            1 5 "CCATGG" "GGTACC") 
COMMENT                      #184=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 60879088 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #185=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #19# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #186=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #19# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 1958 " 1) 
COMMENT                                      #187=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #19# 2) 1 2 2 0 191) 
COMMENT                                            " N2: 2350 " 1))))) "SalI: 2 sites") 
COMMENT                            1 5 "GTCGAC" "CAGCTG"))) 
COMMENT                    "Restriction/Methylation Map") 
COMMENT              #188=(CFolderPar 
COMMENT                    (CGroupPar (CParagraph 14 (0 0) 1 1 0 0 178) 
COMMENT                     (CObjectList 
COMMENT                      #189=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                            "   Similarity between ambiguous nucleotides (if any)" 
COMMENT                            1) 
COMMENT                      #190=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                            "is calculated as an AVERAGE similarity" 1) 
COMMENT                      #191=(CFolderPar 
COMMENT                            (CGroupPar (CParagraph 60913200 (9 0) 1 1 1 0 178) 
COMMENT                             (CObjectList 
COMMENT                              #192=(CGroupPar 
COMMENT                                    (CParagraph 0 (11 #31# 0) 1 2 2 0 180) 
COMMENT                                    (CObjectList 
COMMENT                                     #193=(COlyLinePar 
COMMENT                                           (CLinePar 
COMMENT                                            (CParagraph 0 
COMMENT                                             (17 
COMMENT                                              #194=(COligo "AP-328 (seq pMyNT)" 
COMMENT                                                    "CGCAGTTGTTCTCGCATACC" 
COMMENT                                                    "sequencing primer for pMyNT, binds 3' acetamidase promoter" 
COMMENT                                                    0 (CStringList) 0) 0) 1 2 2 
COMMENT                                             0 181) " CGCAGTTGTTCTCGCATACC" 1)) 
COMMENT                                     #195=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                           "sequencing primer for pMyNT, binds 3' acetamidase promoter" 
COMMENT                                           1))))) "AP-328 (seq pMyNT) (0 sites)") 
COMMENT                      #196=(CFolderPar 
COMMENT                            (CGroupPar (CParagraph 60913248 (9 0) 1 1 1 0 178) 
COMMENT                             (CObjectList 
COMMENT                              #197=(CGroupPar 
COMMENT                                    (CParagraph 0 (11 #32# 0) 1 2 2 0 180) 
COMMENT                                    (CObjectList 
COMMENT                                     #198=(COlyLinePar 
COMMENT                                           (CLinePar 
COMMENT                                            (CParagraph 0 
COMMENT                                             (17 
COMMENT                                              #199=(COligo "AP-329 (seq pMyNT)" 
COMMENT                                                    "CTACATCGCCAGCGCCGACG" "" 0 
COMMENT                                                    (CStringList) 0) 0) 1 2 2 0 
COMMENT                                             181) " CTACATCGCCAGCGCCGACG" 1)))))) 
COMMENT                            "AP-329 (seq pMyNT) (0 sites)") 
COMMENT                      #200=(CFolderPar 
COMMENT                            (CGroupPar (CParagraph 60908736 (9 0) 1 1 1 0 178) 
COMMENT                             (CObjectList 
COMMENT                              #201=(CGroupPar 
COMMENT                                    (CParagraph 0 (11 #33# 0) 1 2 2 0 180) 
COMMENT                                    (CObjectList 
COMMENT                                     #202=(COlyLinePar 
COMMENT                                           (CLinePar 
COMMENT                                            (CParagraph 0 
COMMENT                                             (17 
COMMENT                                              #203=(COligo "AP-330 (seq pMyNT)" 
COMMENT                                                    "CCCTGATTCTGTGGATAACCG" 
COMMENT                                                    "sequencing primer mycobacterial origin of replication " 
COMMENT                                                    0 (CStringList) 0) 0) 1 2 2 
COMMENT                                             0 181) " CCCTGATTCTGTGGATAACCG" 1)) 
COMMENT                                     #204=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                           "sequencing primer mycobacterial origin of replication " 
COMMENT                                           1) 
COMMENT                                     #205=(CLinePar 
COMMENT                                           (CParagraph 0 (2 #33# 1) 1 2 2 0 193) 
COMMENT                                           " N1: position 4082 similarity 100.0%  " 
COMMENT                                           1))))) "AP-330 (seq pMyNT) (1 site)") 
COMMENT                      #206=(CFolderPar 
COMMENT                            (CGroupPar (CParagraph 60911232 (9 0) 1 1 1 0 178) 
COMMENT                             (CObjectList 
COMMENT                              #207=(CGroupPar 
COMMENT                                    (CParagraph 0 (11 #34# 0) 1 2 2 0 180) 
COMMENT                                    (CObjectList 
COMMENT                                     #208=(COlyLinePar 
COMMENT                                           (CLinePar 
COMMENT                                            (CParagraph 0 
COMMENT                                             (17 
COMMENT                                              #209=(COligo "AP-338 (seq pMyNT)" 
COMMENT                                                    "GTTGAGCGGTGGCGCGTCG" 
COMMENT                                                    "sequencing primer for pMy" 
COMMENT                                                    0 (CStringList) 0) 0) 1 2 2 
COMMENT                                             0 181) " GTTGAGCGGTGGCGCGTCG" 1)) 
COMMENT                                     #210=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                           "sequencing primer for pMy" 1))))) 
COMMENT                            "AP-338 (seq pMyNT) (0 sites)") 
COMMENT                      #211=(CFolderPar 
COMMENT                            (CGroupPar (CParagraph 60898944 (9 0) 1 1 1 0 178) 
COMMENT                             (CObjectList 
COMMENT                              #212=(CGroupPar 
COMMENT                                    (CParagraph 0 (11 #35# 0) 1 2 2 0 180) 
COMMENT                                    (CObjectList 
COMMENT                                     #213=(COlyLinePar 
COMMENT                                           (CLinePar 
COMMENT                                            (CParagraph 0 
COMMENT                                             (17 
COMMENT                                              #214=(COligo "AP-340 (seq pMyNT)" 
COMMENT                                                    "CGAGCGCAACGAGACCCTCG" "" 0 
COMMENT                                                    (CStringList) 0) 0) 1 2 2 0 
COMMENT                                             181) " CGAGCGCAACGAGACCCTCG" 1)))))) 
COMMENT                            "AP-340 (seq pMyNT) (0 sites)") 
COMMENT                      #215=(CFolderPar 
COMMENT                            (CGroupPar (CParagraph 60911760 (9 0) 1 1 1 0 178) 
COMMENT                             (CObjectList 
COMMENT                              #216=(CGroupPar 
COMMENT                                    (CParagraph 0 (11 #36# 0) 1 2 2 0 180) 
COMMENT                                    (CObjectList 
COMMENT                                     #217=(COlyLinePar 
COMMENT                                           (CLinePar 
COMMENT                                            (CParagraph 0 
COMMENT                                             (17 
COMMENT                                              #218=(COligo "AP-380 (seq pMyNT)" 
COMMENT                                                    "AACTTACGGAGCTGGTGGGCTC" "" 
COMMENT                                                    0 (CStringList) 0) 0) 1 2 2 
COMMENT                                             0 181) " AACTTACGGAGCTGGTGGGCTC" 1)) 
COMMENT                                     #219=(CLinePar 
COMMENT                                           (CParagraph 0 (2 #36# 1) 1 2 2 0 193) 
COMMENT                                           " N1: position 4179 similarity 100.0% on complementary strand " 
COMMENT                                           1))))) "AP-380 (seq pMyNT) (1 site)") 
COMMENT                      #220=(CFolderPar 
COMMENT                            (CGroupPar (CParagraph 60913008 (9 0) 1 1 1 0 178) 
COMMENT                             (CObjectList 
COMMENT                              #221=(CGroupPar 
COMMENT                                    (CParagraph 0 (11 #37# 0) 1 2 2 0 180) 
COMMENT                                    (CObjectList 
COMMENT                                     #222=(COlyLinePar 
COMMENT                                           (CLinePar 
COMMENT                                            (CParagraph 0 
COMMENT                                             (17 
COMMENT                                              #223=(COligo "AP-386 (seq pMyBAD)" 
COMMENT                                                    "CCTGACGCTTTTTATCGCAACTC" 
COMMENT                                                    "sequencing primer pMyBAD" 0 
COMMENT                                                    (CStringList) 0) 0) 1 2 2 0 
COMMENT                                             181) " CCTGACGCTTTTTATCGCAACTC" 1)) 
COMMENT                                     #224=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                           "sequencing primer pMyBAD" 1) 
COMMENT                                     #225=(CLinePar 
COMMENT                                           (CParagraph 0 (2 #37# 1) 1 2 2 0 193) 
COMMENT                                           " N1: position 1775 similarity 100.0%  " 
COMMENT                                           1))))) "AP-386 (seq pMyBAD) (1 site)") 
COMMENT                      #226=(CFolderPar 
COMMENT                            (CGroupPar (CParagraph 60912720 (9 0) 1 1 1 0 178) 
COMMENT                             (CObjectList 
COMMENT                              #227=(CGroupPar 
COMMENT                                    (CParagraph 0 (11 #38# 0) 1 2 2 0 180) 
COMMENT                                    (CObjectList 
COMMENT                                     #228=(COlyLinePar 
COMMENT                                           (CLinePar 
COMMENT                                            (CParagraph 0 
COMMENT                                             (17 
COMMENT                                              #229=(COligo 
COMMENT                                                    "AP-386rc (seq pMyBAD)" 
COMMENT                                                    "GAGTTGCGATAAAAAGCGTCAGG" 
COMMENT                                                    "binds arabinose promoter, reads into araC gene" 
COMMENT                                                    0 (CStringList) 0) 0) 1 2 2 
COMMENT                                             0 181) " GAGTTGCGATAAAAAGCGTCAGG" 1)) 
COMMENT                                     #230=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                           "binds arabinose promoter, reads into araC gene" 
COMMENT                                           1) 
COMMENT                                     #231=(CLinePar 
COMMENT                                           (CParagraph 0 (2 #38# 1) 1 2 2 0 193) 
COMMENT                                           " N1: position 1775 similarity 100.0% on complementary strand " 
COMMENT                                           1))))) 
COMMENT                            "AP-386rc (seq pMyBAD) (1 site)") 
COMMENT                      #232=(CFolderPar 
COMMENT                            (CGroupPar (CParagraph 60913056 (9 0) 1 1 1 0 178) 
COMMENT                             (CObjectList 
COMMENT                              #233=(CGroupPar 
COMMENT                                    (CParagraph 0 (11 #39# 0) 1 2 2 0 180) 
COMMENT                                    (CObjectList 
COMMENT                                     #234=(COlyLinePar 
COMMENT                                           (CLinePar 
COMMENT                                            (CParagraph 0 
COMMENT                                             (17 
COMMENT                                              #235=(COligo "AP-387 (seq pMy)" 
COMMENT                                                    "TTGATGACGAGCGTAATGGC" 
COMMENT                                                    "binds KanR in pMy (reverse)" 
COMMENT                                                    0 (CStringList) 0) 0) 1 2 2 
COMMENT                                             0 181) " TTGATGACGAGCGTAATGGC" 1)) 
COMMENT                                     #236=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                           "binds KanR in pMy (reverse)" 1))))) 
COMMENT                            "AP-387 (seq pMy) (0 sites)") 
COMMENT                      #237=(CFolderPar 
COMMENT                            (CGroupPar (CParagraph 60912864 (9 0) 1 1 1 0 178) 
COMMENT                             (CObjectList 
COMMENT                              #238=(CGroupPar 
COMMENT                                    (CParagraph 0 (11 #40# 0) 1 2 2 0 180) 
COMMENT                                    (CObjectList 
COMMENT                                     #239=(COlyLinePar 
COMMENT                                           (CLinePar 
COMMENT                                            (CParagraph 0 
COMMENT                                             (17 
COMMENT                                              #240=(COligo "AP-388 (seq pMyBAD)" 
COMMENT                                                    "GACGACCGTAGTGATGAATC" 
COMMENT                                                    "binds araC" 0 (CStringList) 
COMMENT                                                    0) 0) 1 2 2 0 181) 
COMMENT                                            " GACGACCGTAGTGATGAATC" 1)) 
COMMENT                                     #241=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                           "binds araC" 1) 
COMMENT                                     #242=(CLinePar 
COMMENT                                           (CParagraph 0 (2 #40# 1) 1 2 2 0 193) 
COMMENT                                           " N1: position 1250 similarity 100.0%  " 
COMMENT                                           1))))) "AP-388 (seq pMyBAD) (1 site)") 
COMMENT                      #243=(CFolderPar 
COMMENT                            (CGroupPar (CParagraph 60653664 (9 0) 1 1 1 0 178) 
COMMENT                             (CObjectList 
COMMENT                              #244=(CGroupPar 
COMMENT                                    (CParagraph 0 (11 #41# 0) 1 2 2 0 180) 
COMMENT                                    (CObjectList 
COMMENT                                     #245=(COlyLinePar 
COMMENT                                           (CLinePar 
COMMENT                                            (CParagraph 0 
COMMENT                                             (17 
COMMENT                                              #246=(COligo "pMyNT-FWD (MWG)" 
COMMENT                                                    "GGAGTCCACCATGAAGCACC" "" 0 
COMMENT                                                    (CStringList) 0) 0) 1 2 2 0 
COMMENT                                             181) " GGAGTCCACCATGAAGCACC" 1)))))) 
COMMENT                            "pMyNT-FWD (MWG) (0 sites)") 
COMMENT                      #247=(CFolderPar 
COMMENT                            (CGroupPar (CParagraph 60658024 (9 0) 1 1 1 0 178) 
COMMENT                             (CObjectList 
COMMENT                              #248=(CGroupPar 
COMMENT                                    (CParagraph 0 (11 #42# 0) 1 2 2 0 180) 
COMMENT                                    (CObjectList 
COMMENT                                     #249=(COlyLinePar 
COMMENT                                           (CLinePar 
COMMENT                                            (CParagraph 0 
COMMENT                                             (17 
COMMENT                                              #250=(COligo "pMyNT-REV (MWG)" 
COMMENT                                                    "TGGATCTCTCCGGCTTCAC" "" 0 
COMMENT                                                    (CStringList) 0) 0) 1 2 2 0 
COMMENT                                             181) " TGGATCTCTCCGGCTTCAC" 1)) 
COMMENT                                     #251=(CLinePar 
COMMENT                                           (CParagraph 0 (2 #42# 1) 1 2 2 0 193) 
COMMENT                                           " N1: position 2140 similarity 100.0% on complementary strand " 
COMMENT                                           1))))) "pMyNT-REV (MWG) (1 site)"))) 
COMMENT                    "Motifs (both strands)")))) 
COMMENT       (CGraphView 
COMMENT        (CWStyleSheet 
COMMENT         (CObjectList 
COMMENT          #252=(CWidgetStyle "Annotation" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 18 "Times New Roman") 0.7 0 
COMMENT                0 0) 
COMMENT          #253=(CWidgetStyle "Uniq RSite Label" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 18 "Times New Roman") 0.7 0 
COMMENT                1 65535 "@N (@S)" 0) 
COMMENT          #254=(CWidgetStyle "Vanilla" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 0 16777215 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 18 "Times New Roman") 0.8 0 
COMMENT                0 0) 
COMMENT          #255=(CWidgetStyle "Mark 1" 0 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 2 7 48 2 2 "Windings") 0.7 0 0 0) 
COMMENT          #256=(CWidgetStyle "Motif Label" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 18 "Times New Roman") 0.7 0 
COMMENT                1 65535 "@N (@S)" 0) 
COMMENT          #257=(CWidgetStyle "RSite Label" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 18 "Times New Roman") 0.7 0 
COMMENT                1 65535 "@N (@S)" 0) 
COMMENT          #258=(CWidgetStyle "Signal Label" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 1 65535 
COMMENT                "@N" 0) 
COMMENT          #259=(CWidgetStyle "Fragment Label 2" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.7 0 1 65535 
COMMENT                "@F bp (molecule @L bp)" 0) 
COMMENT          #260=(CWidgetStyle "Fragment Label 1" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 1 65535 
COMMENT                "Fragment of @N" 0) 
COMMENT          #261=(CWidgetStyle "Molecule Label 2" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.7 0 1 65535 
COMMENT                "@L bp" 0) 
COMMENT          #262=(CWidgetStyle "Molecule Label 1" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 1 65535 
COMMENT                "@N" 0) 
COMMENT          #263=(CWidgetStyle "Shape GAP" 1 (LOGPEN 1 0 0) 3 1 
COMMENT                (LOGBRUSH 0 16777215 0) 0 0 0) 
COMMENT          #264=(CWidgetStyle "Shape 4" 1 (LOGPEN 0 0 16711680) 1 1 
COMMENT                (LOGBRUSH 2 0 5) 0 0 0) 
COMMENT          #265=(CWidgetStyle "Shape 3" 1 (LOGPEN 0 0 255) 1 1 (LOGBRUSH 2 0 2) 0 
COMMENT                0 0) 
COMMENT          #266=(CWidgetStyle "Shape 2" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 2 65280 4) 
COMMENT                0 0 0) 
COMMENT          #267=(CWidgetStyle "Shape 1" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 2 0 3) 0 0 
COMMENT                0) 
COMMENT          #268=(CWidgetStyle "Shape 0" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 0 0 0) 0 0 
COMMENT                0) 
COMMENT          #269=(CWidgetStyle "Axis" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 0 16777215 0) 
COMMENT                0 0 0) #270=(CWidgetStyle "ORF" 1 (LOGPEN 0 0 0) 8 0 0 0 0) 
COMMENT          #271=(CWidgetStyle "Line 4" 1 (LOGPEN 0 0 65280) 8 0 0 0 0) 
COMMENT          #272=(CWidgetStyle "Line 3" 1 (LOGPEN 0 0 255) 8 0 0 0 0) 
COMMENT          #273=(CWidgetStyle "Line 2" 1 (LOGPEN 0 0 16711680) 8 0 0 0 0) 
COMMENT          #274=(CWidgetStyle "Line 1" 1 (LOGPEN 0 0 0) 1 0 0 0 0) 
COMMENT          #275=(CWidgetStyle "Short Promoter" 1 (LOGPEN 0 0 255) 1 0 0 0 0) 
COMMENT          #276=(CWidgetStyle "RSite" 1 (LOGPEN 0 0 0) 1 0 0 0 0) 
COMMENT          #277=(CWidgetStyle "Motif" 1 (LOGPEN 0 0 0) 1 0 0 0 0) 
COMMENT          #278=(CWidgetStyle "Short Signal" 1 (LOGPEN 0 0 0) 1 0 0 0 0) 
COMMENT          #279=(CWidgetStyle "Line 0" 1 (LOGPEN 0 0 0) 8 0 0 0 0) 
COMMENT          #280=(CWidgetStyle "Void" 0 0 0 0 0) 
COMMENT          #281=(CWidgetStyle "Shape 5" 1 (LOGPEN 0 0 0) 8 1 (LOGBRUSH 0 13311 0) 
COMMENT                1 (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #282=(CWidgetStyle "Shape 6" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 0 13311 0) 
COMMENT                1 (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #283=(CWidgetStyle "Label 0" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 1.05556 
COMMENT                0 1 65535 "@N (@S)" 0) 
COMMENT          #284=(CWidgetStyle "Label 1" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 1.11111 0 1 65535 
COMMENT                "@N" 0) 
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FEATURES             Location/Qualifiers
     source          1..5472
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
                     /vntifkey="98"
     CDS             complement(624..1502)
                     /codon_start=1
                     /gene="AraC other"
                     /vntifkey="4"
                     /label=AraC
                     /note="AraC from pBAD"
     promoter        685..2296
                     /gene="araBAD"
                     /vntifkey="30"
                     /label=araBAD
                     /note="araBAD promoter promoter of the L-arabinose operon of E. coli; the  araC regulatory gene is transcribed in the opposite direction (Guzman et al., 1995)"
     misc_feature    1532..1547
                     /gene="araO2 reg"
                     /vntifkey="21"
                     /label=araO2\reg
                     /note="araO2 reg"
     misc_feature    1731..1744
                     /gene="CAP_BS other"
                     /vntifkey="21"
                     /label=CAP_BS\other
                     /note="CAP_BS other"
     misc_feature    1741..1779
                     /gene="AraI1I2 other"
                     /vntifkey="21"
                     /label=AraI1I2\other
                     /note="AraI1I2 other"
     misc_feature    1776..1804
                     /gene="ARA prom"
                     /vntifkey="21"
                     /label=ARA\prom
                     /note="ARA prom"
     terminator      1997..2043
                     /vntifkey="43"
                     /label=Terminator_1
                     /note="rnnB T1 terminator"
     CDS             complement(2079..3077)
                     /codon_start=1
                     /vntifkey="4"
                     /label=HygR
                     /note="hygR"
     rep_origin      3348..3748
                     /direction=RIGHT
                     /vntifkey="33"
                     /label=Rep_Origin_1
                     /note="ColE1"
     rep_origin      4894..5410
                     /direction=RIGHT
                     /vntifkey="33"
                     /label=Rep_Origin_2
                     /note="mycobact ori"
     misc_feature    685..704
                     /vntifkey="21"
                     /label=sequence-verified
BASE COUNT     1150 a      1636 c      1619 g      1067 t 
ORIGIN
        1 ccgcaaaaag cggcgcgtga ccgccgccga aggcgctcga atcaccggac tatccgaacg 
       61 ccacgtcgtc cggctcgtgg cgcaggaacg cagcgagtgg ctcgccgagc aggctgcacg 
      121 ccgcgaacgc atccgcgcct atcacgacga cgagggccac tcttggccgc aaacggccaa 
      181 acatttcggg ctgcatctgg acaccgttaa gcgactcggc tatcgggcga ggaaagagcg 
      241 tgcggcagaa caggaagcgg ctcaaaaggc ccacaacgaa gccgacaatc caccgctgtt 
      301 ctaacgcaat tggggagcgg gtgtcgcggg ggttccgtgg ggggttccgt tgcaacgggt 
      361 cggacaggta aaagtcctgg tagacgctag ttttctggtt tgggccatgc ctgtctcgtt 
      421 gcgtgtttcg ttgcgcccgt tttgaatacc agccagacga gacggggttc tacgaatctt 
      481 ggtcgatacc aagccatttc cgctgaatat cggggagctc accgccagaa tcggtggttg 
      541 tggtgatgta cgtggcgaac tccgttgtag tgcctgtggt ggcatccgtg gcgcggccgc 
      601 ggtaccagat ctttaaatct agattatgac aacttgacgg ctacatcatt cactttttct 
      661 tcacaaccgg cacggaactc gctcgggctg gccccggtgc attttttaaa tacccgcgag 
      721 aaatagagtt gatcgtcaaa accaacattg cgaccgacgg tggcgatagg catccgggtg 
      781 gtgctcaaaa gcagcttcgc ctggctgata cgttggtcct cgcgccagct taagacgcta 
      841 atccctaact gctggcggaa aagatgtgac agacgcgacg gcgacaagca aacatgctgt 
      901 gcgacgctgg cgatatcaaa attgctgtct gccaggtgat cgctgatgta ctgacaagcc 
      961 tcgcgtaccc gattatccat cggtggatgg agcgactcgt taatcgcttc catgcgccgc 
     1021 agtaacaatt gctcaagcag atttatcgcc agcagctccg aatagcgccc ttccccttgc 
     1081 ccggcgttaa tgatttgccc aaacaggtcg ctgaaatgcg gctggtgcgc ttcatccggg 
     1141 cgaaagaacc ccgtattggc aaatattgac ggccagttaa gccattcatg ccagtaggcg 
     1201 cgcggacgaa agtaaaccca ctggtgatac cattcgcgag cctccggatg acgaccgtag 
     1261 tgatgaatct ctcctggcgg gaacagcaaa atatcacccg gtcggcaaac aaattctcgt 
     1321 ccctgatttt tcaccacccc ctgaccgcga atggtgagat tgagaatata acctttcatt 
     1381 cccagcggtc ggtcgataaa aaaatcgaga taaccgttgg cctcaatcgg cgttaaaccc 
     1441 gccaccagat gggcattaaa cgagtatccc ggcagcaggg gatcattttg cgcttcagcc 
     1501 atacttttca tactcccgcc attcagagaa gaaaccaatt gtccatattg catcagacat 
     1561 tgccgtcact gcgtctttta ctggctcttc tcgctaacca aaccggtaac cccgcttatt 
     1621 aaaagcattc tgtaacaaag cgggaccaaa gccatgacaa aaacgcgtaa caaaagtgtc 
     1681 tataatcacg gcagaaaagt ccacattgat tatttgcacg gcgtcacact ttgctatgcc 
     1741 atagcatttt tatccataag attagcggat cctacctgac gctttttatc gcaactctct 
     1801 actgtttctc catacccgtt ttttgggcta acaggaggaa ttaaccatgc accaccacca 
     1861 ccaccacccg tcggccggcg agaacctgta cttccagggc gccatggacg ataaggtacc 
     1921 ggatccgaat tcgatatcgc tagcaagctt atcgatgtcg acgtagttaa ctagcgtacg 
     1981 atcgactgcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt 
     2041 tatgccatca tggccgcggt gatcagctag agggggcgtc aggcgccggg ggcggtgtcc 
     2101 ggcggccccc agaggaactg cgccagttcc tccggatcgg tgaagccgga gagatccagc 
     2161 ggggtctcct cgaacacctc gaagtcgtgc aggaaggtga aggcgagcag ttcgcgggcg 
     2221 aagtcctcgg tccgcttcca ctgcgccccg tcgagcagcg cggccaggat ctcgcggtcg 
     2281 ccccggaagg cgttgagatg cagttgcacc aggctgtagc gggagtctcc cgcatagacg 
     2341 tcggtgaagt cgacgatccc ggtgacctcg gtcgcggcca ggtccacgaa gatgttggtc 
     2401 ccgtgcaggt cgccgtggac gaaccggggt tcgcggccgg ccagcagcgt gtccacgtcc 
     2461 ggcagccagt cctccaggcg gtccagcagc cggggcgaga ggtagcccca cccgcggtgg 
     2521 tcctcgacgg tcgccgcgcg gcgttcccgc agcagttccg ggaagacctc ggaatggggg 
     2581 gtgagcacgg tgttcccggt cagcggcacc ctgtgcagcc ggccgagcac ccggccgagt 
     2641 tcgcgggcca gggcgagcag cgcgttccgg tcggtcgtgc cgtccatcgc ggaccgccag 
     2701 gtggtgccgg tcatccggct catcaccagg tagggccacg gccaggctcc ggtgccgggc 
     2761 cgcagctcgc cgcggccgag gaggcggggc accggcaccg gggcgtccgc caggaccgcg 
     2821 tacgcctccg actccgacgc gaggctctcc ggaccgcacc agtgctcgcc gaacagcttg 
     2881 atcaccgggc cgggctcgcc gaccagtacg gggttggtgc tctcgccggg cacccgcagc 
     2941 accggcggca ccggcagccc gagctcctcc agggctcggc gggccagcgg ctcccagaat 
     3001 tcctggtcgt tccgcaggct cgcgtaggaa tcatccgaat caatacggtc gagaagtaac 
     3061 agggattctt gtgtcacagc ggacctctat tcacagggta cgggccggct taattccgca 
     3121 cggccggtcg cgacacggcc tgtccgcacc gcggatcagg cgttgacgat gacgggctgg 
     3181 tcggccacgt cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata 
     3241 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag 
     3301 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat 
     3361 ctcatgctct agttccatga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag 
     3421 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg 
     3481 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca 
     3541 gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga 
     3601 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca 
     3661 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc 
     3721 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca 
     3781 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaggggagaa 
     3841 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc 
     3901 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc 
     3961 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg 
     4021 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat 
     4081 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca 
     4141 gccgaacgac cgagcgcaac gcgtgcggcc gcacgcgtga gcccaccagc tccgtaagtt 
     4201 cgggcgctgt gtggctcgta cccgcgcatt caggcggcag ggggtctaac gggtctaagg 
     4261 cggcgtgtac ggccgccaca gcggctctca gcggcccgga aacgtcctcg aaacgacgca 
     4321 tgtgttcctc ctggttggta caggtggttg ggggtgctcg gctgtcgctg gtgttccacc 
     4381 accagggctc gacgggagag cgggggagtg tgcagttgtg gggtggcccc tcagcgaaat 
     4441 atctgacttg gagctcgtgt cggaccatac accggtgatt aatcgtggtc tactaccaag 
     4501 cgtgagccac gtcgccgacg aatttgagca gctctggctg ccgtactggc cgctggcaag 
     4561 cgacgatctg ctcgagggga tctaccgcca aagccgcgcg tcggccctag gccgccggta 
     4621 catcgaggcg aacccaacag cgctggcaaa cctgctggtc gtggacgtag accatccaga 
     4681 cgcagcgctc cgagcgctca gcgcccgggg gtcccatccg ctgcccaacg cgatcgtggg 
     4741 caatcgcgcc aacggccacg cacacgcagt gtgggcactc aacgcccctg ttccacgcac 
     4801 cgaatacgcg cggcgtaagc cgctcgcata catggcggcg tgcgccgaag gccttcggcg 
     4861 cgccgtcgat ggcgaccgca gttactcagg cctcatgacc aaaaaccccg gccacatcgc 
     4921 ctgggaaacg gaatggctcc actcagatct ctacacactc agccacatcg aggccgagct 
     4981 cggcgcgaac atgccaccgc cgcgctggcg tcagcagacc acgtacaaag cggctccgac 
     5041 gccgctaggg cggaattgcg cactgttcga ttccgtcagg ttgtgggcct atcgtcccgc 
     5101 cctcatgcgg atctacctgc cgacccggaa cgtggacgga ctcggccgcg cgatctatgc 
     5161 cgagtgccac gcgcgaaacg ccgaatttcc gtgcaacgac gtgtgtcccg gaccgctacc 
     5221 ggacagcgag gtccgcgcca tcgccaacag catttggcgt tggatcacaa ccaagtcgcg 
     5281 catttgggcg gacgggatcg tggtctacga ggccacactc agtgcgcgcc agtcggccat 
     5341 ctcgcggaag ggcgcagcag cgcgcacggc ggcgagcaca gttgcgcggc gcgcaaagtc 
     5401 cgcgtcagcc atgcatggag gcattgctat gagcgacggc tacagcgacg gctacaaccg 
     5461 gcagccgact gt 
//
