LOCUS       Exported                6836 bp ds-DNA     circular SYN 17-NOV-2016
DEFINITION  Modified pMyNT.
ACCESSION   .
VERSION     .
KEYWORDS    pMyNT-kan
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6836)
  AUTHORS   Wilmanns lab
  TITLE     Direct Submission
  JOURNAL   Exported 17 Nov 2016 from SnapGene Viewer 3.0.2
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6836
                     /organism="synthetic DNA construct"
                     /lab_host="Escherichia coli"
                     /mol_type="other DNA"
     gene            18..659
                     /gene="amiS"
                     /note="amiS"
     CDS             18..659
                     /codon_start=1
                     /transl_table=11
                     /gene="amiS"
                     /product="acetamidase transporter"
                     /note="amiS"
                     /note="amiS AmiS"
                     /db_xref="GI:31339875"
                     /protein_id="DAA01134.1"
                     /translation="MGGVGLFYVGAVLIIDGLMLLGRISPRGATPLNFFVGGLQVVTPT
                     VLILQSGGDAAVIFAASGLYLFGFTYLWVAINNVTDWDGEGLGWFSLFVAIAALGYSWH
                     AFTAEADPAFGVIWLLWAVLWFMLFLLLGLGHDALGPAVGFVAVAEGVITAAVPAFLIV
                     SGNWETGPLPAAVIAVIGFAAVVLAYPIGRRLAAPSVTNPPPAALAATTR"
     terminator      830..876
                     /note="Terminator_1"
                     /note="rnnB T1 terminator"
     CDS             complement(916..1731)
                     /codon_start=1
                     /note="KanR"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPHAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHNLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     rep_origin      2115..2515
                     /direction=RIGHT
                     /note="Rep_Origin_1"
                     /note="ColE1"
     rep_origin      3661..4177
                     /direction=RIGHT
                     /note="Rep_Origin_2"
                     /note="mycobact ori"
     promoter        4881..659
                     /note="Promoter_P_1"
                     /note="acetamidase promoter"
     gene            complement(4881..5954)
                     /gene="amiC"
                     /note="amiC"
     CDS             complement(4881..5954)
                     /codon_start=1
                     /transl_table=11
                     /gene="amiC"
                     /product="acetamidase regulator"
                     /note="amiC"
                     /note="amiC AmiC"
                     /db_xref="GI:31339872"
                     /protein_id="DAA01131.1"
                     /translation="VQDGEVEFRVGLVIPLQGPAGIFAPSCEAVAELAAKEVNDRGGLQ
                     GRKVTIEVLDGGAPGDDVARTVADRLRGHGLDAVTGWHISAVRNRISPVVRDRIPYVYT
                     SLYEGGERTPGVFCTGETPQIQIAPALAWLRDHFGIRSWCLVGDDYIWPRRSAAAARAY
                     CRDLDLELRREIYVPYGTDDFRAPVRKAIASGAQAVLMLLVGQDAVLFNREFARAGGHD
                     RMARFSPLMEENMLLASGAGSTENLYVAAAYFSSLATAGAMDLMGSYVARYGADAPPLN
                     AMAESCYEGLLALEAIFQRAHSPEIPDLMASAHDVGFDGPRGPMCMRDSQFDQQVYIAS
                     ADGYDFDILDTLATLDA"
     gene            6055..6483
                     /gene="amiA"
                     /note="amiA"
     CDS             6055..6483
                     /codon_start=1
                     /transl_table=11
                     /gene="amiA"
                     /product="acetamidase regulator"
                     /note="amiA"
                     /note="amiA marR family of regulators; AmiA"
                     /db_xref="GI:31339873"
                     /protein_id="DAA01132.1"
                     /translation="MAAGQQRRPNLLLPLVRLTHLAESAIERVLADSSLKIEDWRVLDE
                     LAGRRTVPMSDLAQATLITGPTLTRTVDRLVSQGIIYRTADLHDRRRVLVALTPRGRTL
                     RNRLVDAVAEAECAAFESCGLDVDQLRELVDTTSNLTS"
     gene            6565..13
                     /gene="amiD"
                     /note="amiD"
     CDS             6565..13
                     /codon_start=1
                     /transl_table=11
                     /gene="amiD"
                     /product="acetamidase regulator"
                     /note="amiD"
                     /note="amiD AmiD"
                     /db_xref="GI:31339874"
                     /protein_id="DAA01133.1"
                     /translation="MPTYTFRCSHCGPFDLTCAISERDAAATCPECRTPARRVFGSVGL
                     TTFTAGHHRAFDAASASAESPTVVKSIPAGADRPRAPRRNPGLPSLPRY"
ORIGIN
        1 gccgaggtac tagcgacatg ggtggcgtcg ggctcttcta cgtgggtgcg gtgctcatca
       61 tcgacgggct gatgctgctg ggccgcatca gcccacgagg cgcaacaccg ctgaacttct
      121 tcgtcggcgg actgcaggtg gtgacgccta cggtgctgat cctgcagtcc ggcggagacg
      181 cggccgtgat cttcgcggcc tccgggctct acctgttcgg cttcacctac ctgtgggtgg
      241 ccatcaacaa cgtgaccgac tgggacggag aaggtctcgg atggttctcg ctgttcgtcg
      301 cgatcgccgc actcggctac tcgtggcacg cgttcaccgc cgaggccgac ccggcgttcg
      361 gggtgatctg gctgctgtgg gcagtgctgt ggttcatgct gttcctgctg ctcggcctgg
      421 ggcacgacgc actggggccc gccgtcgggt tcgtcgcggt ggccgaaggc gtgatcaccg
      481 ccgccgtgcc ggccttcctg atcgtgtcgg gcaactggga aaccggcccg ctccccgccg
      541 cggtcatcgc cgtgatcggt tttgccgcag ttgttctcgc ataccccatc gggcgccgtc
      601 tcgcagcgcc gtcagtcacc aaccctccac cggccgcgct cgcggccacc acccgataag
      661 agaaagggag tccaccatga agcaccacca ccaccaccac ccgtcggccg gcgagaacct
      721 gtacttccag ggcgccatgg acgataaggt accggatccg aattcgatat cgctagcaag
      781 cttatcgatg tcgacgtagt taactagcgt acgatcgact gccaggcatc aaataaaacg
      841 aaaggctcag tcgaaagact gggcctttcg ttttatgcca tcatggccgc ggtgatcagc
      901 tagagggggc gctgattaga aaaactcatc gagcatcaaa tgaaactgca atttattcat
      961 atcaggatta tcaataccat atttttgaaa aagccgtttc tgtaatgaag gagaaaactc
     1021 accgaggcag ttccatagga tggcaagatc ctggtatcgg tctgcgattc cgactcgtcc
     1081 aacatcaata caacctatta atttcccctc gtcaaaaata aggttatcaa gtgagaaatc
     1141 accatgagtg acgactgaat ccggtgagaa tggcaaaaga ttatgcattt ctttccagac
     1201 ttgttcaaca ggccagccat tacgctcgtc atcaaaatca ctcgcatcaa ccaaaccgtt
     1261 attcattcgt gattgcgcct gagcgagacg aaatacgcga tcgctgttaa aaggacaatt
     1321 acaaacagga atcgaatgca accggcgcag gaacactgcc agcgcatcaa caatattttc
     1381 acctgaatca ggatattctt ctaatacctg gaatgctgtt ttcccgggga tcgcagtggt
     1441 gagtaaccat gcatcatcag gagtacggat aaaatgcttg atggtcggaa gaggcataaa
     1501 ttccgtcagc cagtttagtc tgaccatctc atctgtaaca tcattggcaa cgctaccttt
     1561 gccatgtttc agaaacaact ctggcgcatg gggcttccca tacaagcgat agattgtcgc
     1621 acctgattgc ccgacattat cgcgagccca tttataccca tataaatcag catccatgtt
     1681 ggaatttaat cgcggcctcg agcaagacgt ttcccgttga atatggctca taacacccct
     1741 tgtattactg tttatgtaag cagacagttt tattgttcat gatgatatat ttttatcttg
     1801 tgcaatgtaa catcagagat tttgagacac aacgtcgctt tgttagcgga cctctattca
     1861 cagggtacgg gccggcttaa ttccgcacgg ccggtcgcga cacggcctgt ccgcaccgcg
     1921 gatcaggcgt tgacgatgac gggctggtcg gccacgtcgg ggagtcaggc aactatggat
     1981 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
     2041 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     2101 atctaggtga agatcctttt tgataatctc atgctctagt tccatgagcg tcagaccccg
     2161 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     2221 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     2281 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
     2341 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     2401 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     2461 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     2521 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     2581 aaagcgccac gcttcccgag gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     2641 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     2701 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     2761 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     2821 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     2881 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcaacgcg tgcggccgca
     2941 cgcgtgagcc caccagctcc gtaagttcgg gcgctgtgtg gctcgtaccc gcgcattcag
     3001 gcggcagggg gtctaacggg tctaaggcgg cgtgtacggc cgccacagcg gctctcagcg
     3061 gcccggaaac gtcctcgaaa cgacgcatgt gttcctcctg gttggtacag gtggttgggg
     3121 gtgctcggct gtcgctggtg ttccaccacc agggctcgac gggagagcgg gggagtgtgc
     3181 agttgtgggg tggcccctca gcgaaatatc tgacttggag ctcgtgtcgg accatacacc
     3241 ggtgattaat cgtggtctac taccaagcgt gagccacgtc gccgacgaat ttgagcagct
     3301 ctggctgccg tactggccgc tggcaagcga cgatctgctc gaggggatct accgccaaag
     3361 ccgcgcgtcg gccctaggcc gccggtacat cgaggcgaac ccaacagcgc tggcaaacct
     3421 gctggtcgtg gacgtagacc atccagacgc agcgctccga gcgctcagcg cccgggggtc
     3481 ccatccgctg cccaacgcga tcgtgggcaa tcgcgccaac ggccacgcac acgcagtgtg
     3541 ggcactcaac gcccctgttc cacgcaccga atacgcgcgg cgtaagccgc tcgcatacat
     3601 ggcggcgtgc gccgaaggcc ttcggcgcgc cgtcgatggc gaccgcagtt actcaggcct
     3661 catgaccaaa aaccccggcc acatcgcctg ggaaacggaa tggctccact cagatctcta
     3721 cacactcagc cacatcgagg ccgagctcgg cgcgaacatg ccaccgccgc gctggcgtca
     3781 gcagaccacg tacaaagcgg ctccgacgcc gctagggcgg aattgcgcac tgttcgattc
     3841 cgtcaggttg tgggcctatc gtcccgccct catgcggatc tacctgccga cccggaacgt
     3901 ggacggactc ggccgcgcga tctatgccga gtgccacgcg cgaaacgccg aatttccgtg
     3961 caacgacgtg tgtcccggac cgctaccgga cagcgaggtc cgcgccatcg ccaacagcat
     4021 ttggcgttgg atcacaacca agtcgcgcat ttgggcggac gggatcgtgg tctacgaggc
     4081 cacactcagt gcgcgccagt cggccatctc gcggaagggc gcagcagcgc gcacggcggc
     4141 gagcacagtt gcgcggcgcg caaagtccgc gtcagccatg catggaggca ttgctatgag
     4201 cgacggctac agcgacggct acaaccggca gccgactgtc cgcaaaaagc ggcgcgtgac
     4261 cgccgccgaa ggcgctcgaa tcaccggact atccgaacgc cacgtcgtcc ggctcgtggc
     4321 gcaggaacgc agcgagtggc tcgccgagca ggctgcacgc cgcgaacgca tccgcgccta
     4381 tcacgacgac gagggccact cttggccgca aacggccaaa catttcgggc tgcatctgga
     4441 caccgttaag cgactcggct atcgggcgag gaaagagcgt gcggcagaac aggaagcggc
     4501 tcaaaaggcc cacaacgaag ccgacaatcc accgctgttc taacgcaatt ggggagcggg
     4561 tgtcgcgggg gttccgtggg gggttccgtt gcaacgggtc ggacaggtaa aagtcctggt
     4621 agacgctagt tttctggttt gggccatgcc tgtctcgttg cgtgtttcgt tgcgcccgtt
     4681 ttgaatacca gccagacgag acggggttct acgaatcttg gtcgatacca agccatttcc
     4741 gctgaatatc ggggagctca ccgccagaat cggtggttgt ggtgatgtac gtggcgaact
     4801 ccgttgtagt gcctgtggtg gcatccgtgg cgcggccgcg gtaccagatc tttaaatcta
     4861 gataaagaag tgacgcggtc tcaagcgtcg agcgtcgcca gcgtgtcgag gatgtcgaag
     4921 tcgtagccgt cggcgctggc gatgtagacc tgctggtcga attgactgtc gcgcatacac
     4981 atcgggcccc ggggcccgtc gaacccgaca tcgtgcgcgg atgccatcag gtccggtatc
     5041 tcgggggagt gggcccgctg gaagatggcc tcgagcgcaa gcagaccctc gtaacaggat
     5101 tcggccatcg cgttgagcgg tggcgcgtcg gcgccgtagc gggcgacgta gctgcccatc
     5161 aggtccatcg cacccgcggt ggccagtgaa ctgaagtacg ccgcggcgac atagaggttt
     5221 tcggtggagc cggcgccgct ggccagcagc atgttctcct ccatcagcgg gctgaaccgc
     5281 gccatgcggt cgtgcccgcc ggcgcgcgcg aactcgcggt tgaacaacac ggcgtcctgg
     5341 ccgacgagca gcatcaacac ggcctgcgcc cccgacgcga tggccttgcg gacaggtgcg
     5401 cggaaatcgt cggtgccgta cgggacgtag atctcccgtc tgagctcgag gtccagatct
     5461 cggcagtacg cgcgggcggc cgcggcggaa cggcgcggcc agatgtagtc atcgccgacc
     5521 aggcaccagg accggatgcc gaagtggtcg cgcagccagg cgagcgcggg cgcgatctgg
     5581 atctgcggtg tctcgcctgt gcagaacacg cccggtgtgc gttcaccgcc ctcgtacaac
     5641 gaggtgtaga cgtacgggat gcggtcgcgg accaccgggg agatgcggtt gcgcacggcc
     5701 gagatgtgcc agccggtcac ggcgtcgaga ccgtgacctc gcaaccggtc ggcgacggtc
     5761 cgggcgacgt cgtcgccggg cgctccgccg tcgagcacct cgatggtgac cttgcggccc
     5821 tgcaggccgc ctcggtcgtt gacctccttg gccgcgagct cggccacggc ctcgcacgaa
     5881 ggcgcgaaga ttcccgctgg cccttgaagc ggaatcacca gcccgacgcg gaactcaacc
     5941 tcgccgtcct gcactccaga tcaccgtcga tcccgtgtag tctgcgcttc aaaggtttct
     6001 agcagaaata attcattctg aacagacccc gccgtcgaca cgaggagacc caacatggcc
     6061 gccggacagc agcgccgccc caacctcctg ctgccgttgg tgcgtctgac ccacctcgcg
     6121 gagtcggcga tcgaacgcgt gctcgcggac tcgtcgctca agatcgagga ctggcgcgtg
     6181 ctcgacgagt tggccggacg gcgcaccgtg cccatgagcg atctcgcgca ggccacgctg
     6241 atcacgggtc cgactctcac cagaaccgtc gatcgccttg tgtcgcaagg gatcatctac
     6301 cggactgccg atctgcatga ccgccggcgg gtgctcgtgg cgttgacccc gcgggggcgg
     6361 acgctgcgca accgcctggt ggacgcggta gccgaggccg agtgtgcggc ttttgaatcg
     6421 tgcgggctgg acgtcgacca gttgcgcgaa ctcgtcgaca ccacctcgaa tttgacttcg
     6481 taaccacccg cgcccggcgc gggcgttcac ccttgacttt tattttcatc tggatatatt
     6541 tcgggtgaat ggaaaggggt gaccatgccg acctacacat tccgttgttc ccactgcggt
     6601 cccttcgatc tcacctgcgc gatctccgag cgcgatgcgg cggcgacctg tccggagtgc
     6661 cggacgccgg cgcgccgggt cttcggttcg gtagggctga cgacattcac cgcgggacat
     6721 caccgcgcat tcgacgcggc gtccgcgagc gccgaaagtc ccacggtggt gaagtcgatt
     6781 cccgcaggcg cggaccgccc gcgggccccg cgccgcaatc ccggtctacc gagtct
//
