LOCUS       Exported               19435 bp ds-DNA     circular SYN 08-MAR-2017
DEFINITION  pGEMINI_Yellow:FVM_35S:Red.
ACCESSION   .
VERSION     .
KEYWORDS    pGEMINI_Yellow FVM_35S Red
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 19435)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported 08 Mar 2017 from SnapGene 2.8.3
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..19435
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          8023..9702
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /note="color: #ffffff"
     CDS             complement(145..516)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /note="traJ"
                     /note="color: #993366"
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            549..658
                     /note="incP origin of transfer"
                     /note="color: #a6acb3"
     CDS             complement(1339..1989)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /note="TetR"
                     /note="color: #993366"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     misc_feature    2454..2478
                     /note="RB T-DNA repeat"
                     /note="right border repeat from nopaline C58 T-DNA"
                     /note="color: #ffe4c4"
     promoter        2602..2781
                     /note="NOS promoter"
                     /note="nopaline synthase promoter"
                     /note="color: #ffffff; direction: RIGHT"
     CDS             2838..3632
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /note="NeoR/KanR"
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /note="color: #ccffcc"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     terminator      4022..4274
                     /note="NOS terminator"
                     /note="nopaline synthase terminator and poly(A) signal"
                     /note="color: #ffffff"
     protein_bind    4823..4844
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding site"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
                     /note="color: #31849b"
     promoter        4859..4889
                     /note="lac promoter"
                     /note="promoter for the E. coli lac operon"
                     /note="This forward directional feature has 3 segments:
                        1: 4859 .. 4864 / #ffffff / -35
                        2: 4865 .. 4882 / #ffffff
                        3: 4883 .. 4889 / #ffffff / -10"
     protein_bind    4897..4913
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
     primer_bind     4921..4937
                     /note="M13 rev"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: RIGHT"
     terminator      4968..5220
                     /note="NOS terminator"
                     /note="nopaline synthase terminator and poly(A) signal"
                     /note="color: #ffffff"
     protein_bind    5295..5319
                     /gene="mutant version of attB"
                     /bound_moiety="BP Clonase(TM)"
                     /note="attB2"
                     /note="recombination site for the Gateway(R) BP reaction"
                     /note="color: #31849b; direction: RIGHT"
     CDS             complement(5336..6454)
                     /codon_start=1
                     /note="sAPX (At4g08390)"
                     /note="color: #993366"
                     /translation="MAERVSLTLNGTLLSPPPTTTTTTMSSSLRSTTAASLLLRSSSSS
                     SRSTLTLSASSSLSFVRSLVSSPRLSSSSSLSQKKYRIASVNRSFNSTTAATKSSSSDP
                     DQLKNAREDIKELLSTKFCHPILVRLGWHDAGTYNKNIKEWPQRGGANGSLRFDIELKH
                     AANAGLVNALNLIKDIKEKYSGISYADLFQLASATAIEEAGGPKIPMKYGRVDASGPED
                     CPEEGRLPDAGPPSPATHLREVFYRMGLDDKDIVALSGAHTLGRSRPERSGWGKPETKY
                     TKEGPGAPGGQSWTPEWLKFDNSYFKEIKEKRDEDLLVLPTDAAIFEDSSFKVYAEKYA
                     ADQDAFFKDYAVAHAKLSNLGAEFNPPEGIVI"
     protein_bind    complement(6475..6499)
                     /gene="mutant version of attB"
                     /bound_moiety="BP Clonase(TM)"
                     /note="attB1"
                     /note="recombination site for the Gateway(R) BP reaction"
                     /note="color: #31849b; direction: LEFT"
     promoter        6510..6856
                     /note="FMV 34S promoter"
                     /note="color: #ffffff"
     promoter        7611..7956
                     /note="CaMV 35S promoter"
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
                     /note="color: #ffffff; direction: RIGHT"
     protein_bind    7978..8002
                     /gene="mutant version of attB"
                     /bound_moiety="BP Clonase(TM)"
                     /note="attB1"
                     /note="recombination site for the Gateway(R) BP reaction"
                     /note="color: #31849b; direction: RIGHT"
     CDS             8023..9702
                     /codon_start=1
                     /note="RbcS:HyPer2"
                     /note="This feature has 3 segments:
                        1: 8023 .. 8262 / #00ff00 / RbcS
                        2: 8263 .. 8268 / #993366
                        3: 8269 .. 9702 / #ffff00 / HyPer2"
                     /translation="MASSMLSSATMVASPAQATMVAPFNGLKSSAAFPATRKANNDITS
                     ITSNGGRVNCMQVWPPIGKKKFETLSYLPDLTDSEKLEMASQQGETMSGPLHIGLIPTV
                     GPYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVPMADLAGEKLLMLEDGHCLRDQAMSAGYNSDNVYIMAD
                     KQKNGIKANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSFQSVLSKDPNEK
                     RDHMVLLEFVTAAGITLGMDELYNVDGGSGGTGSKGEELFTGVVPILVELDGDVNGHKF
                     SVSGEGEGDATYGKLTLKLICTTGKLPVPWPTLVTTLGYGLKCFARYPDHMKQHDFFKS
                     AMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIGFKEDGNILGHKLEYNG
                     TGFCFEAGADEDTHFRATSLETLRNMVAVGSGITLLPALAVPPERKRDGVVYLPCIKPE
                     PRRTIGLVYRPGSPLRSRYEQLAEAIRARMDGHFDKVLKQAV"
     protein_bind    complement(9719..9743)
                     /gene="mutant version of attB"
                     /bound_moiety="BP Clonase(TM)"
                     /note="attB2"
                     /note="recombination site for the Gateway(R) BP reaction"
                     /note="color: #31849b; direction: LEFT"
     terminator      9775..10027
                     /note="NOS terminator"
                     /note="nopaline synthase terminator and poly(A) signal"
                     /note="color: #ffffff"
     terminator      10456..10708
                     /note="NOS terminator"
                     /note="nopaline synthase terminator and poly(A) signal"
                     /note="color: #ffffff"
     CDS             complement(11176..12201)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /note="HygR"
                     /note="confers resistance to hygromycin"
                     /note="color: #ccffcc"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(12211..12555)
                     /note="CaMV 35S promoter"
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
                     /note="color: #ffffff; direction: LEFT"
     primer_bind     complement(12665..12681)
                     /note="M13 fwd"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: LEFT"
     misc_feature    13299..13323
                     /note="LB T-DNA repeat"
                     /note="left border repeat from nopaline C58 T-DNA"
                     /note="color: #ffe4c4"
     CDS             complement(14919..16067)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /note="trfA"
                     /note="color: #993366"
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(16366..17160)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /note="KanR"
                     /note="confers resistance to kanamycin"
                     /note="color: #ccffcc"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     mobile_element  17352..18119
                     /mobile_element_type="insertion sequence:IS1"
                     /note="IS1"
                     /note="prokaryotic transposable element"
                     /note="color: #ffe4c4"
     rep_origin      18742..18
                     /note="oriV"
                     /note="incP origin of replication"
                     /note="color: #ffff00"
ORIGIN
        1 tgagcgtcgc aaaggcgctc ggtcttgcct tgctcgtcgg tgatgtactt caccagctcc
       61 gcgaagtcgc tcttcttgat ggagcgcatg gggacgtgct tggcaatcac gcgcaccccc
      121 cggccgtttt agcggctaaa aaagtcatgg ctctgccctc gggcggacca cgcccatcat
      181 gaccttgcca agctcgtcct gcttctcttc gatcttcgcc agcagggcga ggatcgtggc
      241 atcaccgaac cgcgccgtgc gcgggtcgtc ggtgagccag agtttcagca ggccgcccag
      301 gcggcccagg tcgccattga tgcgggccag ctcgcggacg tgctcatagt ccacgacgcc
      361 cgtgattttg tagccctggc cgacggccag caggtaggcc gacaggctca tgccggccgc
      421 cgccgccttt tcctcaatcg ctcttcgttc gtctggaagg cagtacacct tgataggtgg
      481 gctgcccttc ctggttggct tggtttcatc agccatccgc ttgccctcat ctgttacgcc
      541 ggcggtagcc ggccagcctc gcagagcagg attcccgttg agcaccgcca ggtgcgaata
      601 agggacagtg aagaaggaac acccgctcgc gggtgggcct acttcaccta tcctgcccgg
      661 ctgacgccgt tggatacacc aaggaaagtc tacacgaacc ctttggcaaa atcctgtata
      721 tcgtgcgaaa aaggatggat ataccgaaaa aatcgctata atgaccccga agcagggtta
      781 tgcagcggaa aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
      841 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
      901 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
      961 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     1021 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     1081 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     1141 cagtgagcga ggaagcggaa gagcgccaga aggccgccag agaggccgag cgcggccgtg
     1201 aggcttggac gctagggcag ggcatgaaaa agcccgtagc gggctgctac gggcgtctga
     1261 cgcggtggaa agggggaggg gatgttgtct acatggctct gctgtagtga gtgggttgcg
     1321 ctccggcagc ggtcctgatc aatcgtcacc ctttctcggt ccttcaacgt tcctgacaac
     1381 gagcctcctt ttcgccaatc catcgacaat caccgcgagt ccctgctcga acgctgcgtc
     1441 cggaccggct tcgtcgaagg cgtctatcgc ggcccgcaac agcggcgaga gcggagcctg
     1501 ttcaacggtg ccgccgcgct cgccggcatc gctgtcgccg gcctgctcct caagcacggc
     1561 cccaacagtg aagtagctga ttgtcatcag cgcattgacg gcgtccccgg ccgaaaaacc
     1621 cgcctcgcag aggaagcgaa gctgcgcgtc ggccgtttcc atctgcggtg cgcccggtcg
     1681 cgtgccggca tggatgcgcg cgccatcgcg gtaggcgagc agcgcctgcc tgaagctgcg
     1741 ggcattcccg atcagaaatg agcgccagtc gtcgtcggct ctcggcaccg aatgcgtatg
     1801 attctccgcc agcatggctt cggccagtgc gtcgagcagc gcccgcttgt tcctgaagtg
     1861 ccagtaaagc gccggctgct gaacccccaa ccgttccgcc agtttgcgtg tcgtcagacc
     1921 gtctacgccg acctcgttca acaggtccag ggcggcacgg atcactgtat tcggctgcaa
     1981 ctttgtcatg cttgacactt tatcactgat aaacataata tgtccaccaa cttatcagtg
     2041 ataaagaatc cgcgcgttca atcggaccag cggaggctgg tccggaggcc agacgtgaaa
     2101 cccaacatac ccctgatcgt aattctgagc actgtcgcgc tcgacgctgt cggcatcggc
     2161 ctgattatgc cggtgctgcc gggcctcctg cgcgatctgg ttcactcgaa cgacgtcacc
     2221 gcccactatg gcattctgct ggcgctgtat gcgttggtgc aatttgcctg cgcacctgtg
     2281 ctgggcgcgc tgtcggatcg tttcgggcgg cggccaatct tgctcgtctc gctggccggc
     2341 gccagatctg gggaaccctg tggttggcat gcacatacaa atggacgaac ggataaacct
     2401 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
     2461 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
     2521 tcatgagcgg agaattaagg gagtcacgtt atgacccccg ccgatgacgc gggacaagcc
     2581 gttttacgtt tggaactgac agaaccgcaa cgttgaagga gccactcagc cgcgggtttc
     2641 tggagtttaa tgagctaagc acatacgtca gaaaccatta ttgcgcgttc aaaagtcgcc
     2701 taaggtcact atcagctagc aaatatttct tgtcaaaaat gctccactga cgttccataa
     2761 attcccctcg gtatccaatt agagtctcat attcactctc aatccaaata atctgcaccg
     2821 gatctggatc gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt
     2881 gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg
     2941 ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg
     3001 gtgccctgaa tgaactgcag gacgaggcag cgcggctatc gtggctggcc acgacgggcg
     3061 ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg
     3121 gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca
     3181 tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc
     3241 accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc
     3301 aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca
     3361 aggcgcgcat gcccgacggc gatgatctcg tcgtgaccca tggcgatgcc tgcttgccga
     3421 atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg
     3481 cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg
     3541 aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg
     3601 ccttctatcg ccttcttgac gagttcttct gagcgggact ctggggttcg aaatgaccga
     3661 ccaagcgacg cccaacctgc catcacgaga tttcgattcc accgccgcct tctatgaaag
     3721 gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct
     3781 catgctggag ttcttcgccc acgggatctc tgcggaacag gcggtcgaag gtgccgatat
     3841 cattacgaca gcaacggccg acaagcacaa cgccacgatc ctgagcgaca atatgatcgg
     3901 gcccggcgtc cacatcaacg gcgtcggcgg cgactgccca ggcaagaccg agatgcaccg
     3961 cgatatcttg ctgcgttcgg atattttcgt ggagttcccg ccacagaccc ggatgatccc
     4021 cgatcgttca aacatttggc aataaagttt cttaagattg aatcctgttg ccggtcttgc
     4081 gatgattatc atataatttc tgttgaatta cgttaagcat gtaataatta acatgtaatg
     4141 catgacgtta tttatgagat gggtttttat gattagagtc ccgcaattat acatttaata
     4201 cgcgatagaa aacaaaatat agcgcgcaaa ctaggataaa ttatcgcgcg cggtgtcatc
     4261 tatgttacta gatcgggcct cctgtcaatg ctggcggcgg ctctggtggt ggttctggtg
     4321 gcggctctga gggtggtggc tctgagggtg gcggttctga gggtggcggc tctgagggag
     4381 gcggttccgg tggtggctct ggttccggtg attttgatta tgaaaagatg gcaaacgcta
     4441 ataagggggc tatgaccgaa aatgccgatg aaaacgcgct acagtctgac gctaaaggca
     4501 aacttgattc tgtcgctact gattacggtg ctgctatcga tggtttcatt ggtgacgttt
     4561 ccggccttgc taatggtaat ggtgctactg gtgattttgc tggctctaat tcccaaatgg
     4621 ctcaagtcgg tgacggtgat aattcacctt taatgaataa tttccgtcaa tatttacctt
     4681 ccctccctca atcggttgaa tgtcgccctt ttgtctttgg cccaatacgc aaaccgcctc
     4741 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
     4801 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt
     4861 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
     4921 caggaaacag ctatgaccat gattacgcca agcttcctag gatccccgat ctagtaacat
     4981 agatgacacc gcgcgcgata atttatccta gtttgcgcgc tatattttgt tttctatcgc
     5041 gtattaaatg tataattgcg ggactctaat cataaaaacc catctcataa ataacgtcat
     5101 gcattacatg ttaattatta catgcttaac gtaattcaac agaaattata tgataatcat
     5161 cgcaagaccg gcaacaggat tcaatcttaa gaaactttat tgccaaatgt ttgaacgatc
     5221 gggaattgga tccggtaccg agctcggatc cactagtaac ggccgccagt gtgctggaat
     5281 tcgcccttgg atccaccact ttgtacaaga aagctgggtc ggcgcgccca cccttttaga
     5341 taacgatacc ctccggaggg ttaaattctg ctccaagatt gctgagcttc gcatgggcta
     5401 cagcgtaatc cttgaaaaat gcatcctgat ctgcagcata cttttcagca tagaccttga
     5461 aagaagaatc ttcgaagatg gcagcatcag tgggtaggac aaggagatct tcatctctct
     5521 tttccttgat ctccttgaag taagaattat caaacttcag ccactctggt gtccatgact
     5581 gtcctcctgg tgctccaggt ccttctttcg tgtacttcgt ctcaggcttc ccccaaccac
     5641 tacgttctgg cctagatctt cctaacgtgt gagcaccaga taatgcaact atgtccttgt
     5701 catctaatcc cattctgtaa aaaacttctc tgagatgagt agcaggtgaa ggaggaccag
     5761 catctggaag ccttccttct tctggacaat cctcaggacc agaggcatca actctaccat
     5821 atttcatcgg tattttcggt cctccagctt cctctatagc agtagcacta gctaactgga
     5881 acaaatcagc ataactgatc ccagagtatt tctctttgat atccttaatc aagtttaaag
     5941 cattcacaag accagcgtta gcagcatgtt taagctcaat atcgaatctc aaacttccat
     6001 tagctccacc tctttgtggc cattccttaa tgttcttgtt atacgtacca gcatcatgcc
     6061 aacctaatct aaccaaaatc ggatggcaga atttggtgct gagaagctct ttgatatctt
     6121 ctctagcgtt cttcaactga tcaggatctg aagatgaaga tttagtagcg gcggtggtgg
     6181 agttgaaact ccgattcacc gacgcaattc gatatttctt ctgagagagg gatgaagaag
     6241 aagagagacg tggagaagag acgagagatc tgacgaagga gagagaggag gaagcggaga
     6301 gagtgagagt ggatctggag gaggaggagg aggagcggag gagaagagaa gcggcggtgg
     6361 tagatcggag agaagaagac attgttgttg ttgttgttgt gggaggagga gaaaggaggg
     6421 ttccgttgag tgtgagagac acacgctctg ccatggtgaa gggggcggcc gcggagcctg
     6481 cttttttgta caaacttgtt ctagaaagga ttggttcagt atctgatgat ccttcaaatg
     6541 ggaatgaatg ccttcttata tagagggaat tcttttgtgg tcgtcactgc gttcgtcata
     6601 cgcattagtg agtgggctgt caggacagct cttttccacg ttattttgtt ccccacttgt
     6661 actagaggaa tctgctttat ctttgcaata aaggcaaaga tgcttttggt aggtgcgcct
     6721 aacaattctg caccattcct tttttgtctg gtccccacaa gccagctgct cgatgttgac
     6781 aagattactt tcaaagatgc ccactaactt taagtcttcg gtggatgtct ttttctgaaa
     6841 cttactgacc atgatgcatg tgctggaaca gtagtttact ttgattgaag attcttcatt
     6901 gatctcctgt agcttttggc taatggtttg gagactctgt accctgacct tgttgaggct
     6961 ttggactgag aattcttcct tacaaacctt tgaggatggg agttccttct tggttttggc
     7021 gataccaatt tgaataaagt gatatggctc gtaccttgtt gattgaaccc aatctggaat
     7081 gctgctaaat cctgagctca agcttgcatg cctgcaggtc cccagattag ccttttcaat
     7141 ttcagaaaga atgctaaccc acagatggtt agagaggctt acgcagcagg tctcatcaag
     7201 acgatctacc cgagcaataa tctccaggaa atcaaatacc ttcccaagaa ggttaaagat
     7261 gcagtcaaaa gattcaggac taactgcatc aagaacacag agaaagatat atttctcaag
     7321 atcagaagta ctattccagt atggacgatt caaggcttgc ttcacaaacc aaggcaagta
     7381 atagagattg gagtctctaa aaaggtagtt cccactgaat caaaggccat ggagtcaaag
     7441 attcaaatag aggacctaac agaactcgcc gtaaagactg gcgaacagtt catacagagt
     7501 ctcttacgac tcaatgacaa gaagaaaatc ttcgtcaaca tggtggagca cgacacactt
     7561 gtctactcca aaaatatcaa agatacagtc tcagaagacc aaagggcaat tgagactttt
     7621 caacaaaggg taatatccgg aaacctcctc ggattccatt gcccagctat ctgtcacttt
     7681 attgtgaaga tagtggaaaa ggaaggtggc tcctacaaat gccatcattg cgataaagga
     7741 aaggccatcg ttgaagatgc ctctgccgac agtggtccca aagatggacc cccacccacg
     7801 aggagcatcg tggaaaaaga agacgttcca accacgtctt caaagcaagt ggattgatgt
     7861 gatatctcca ctgacgtaag ggatgacgca caatcccact atccttcgca agacccttcc
     7921 tctatataag gaagttcatt tcatttggag agaacacggg ggactctaga gttatcaaca
     7981 agtttgtaca aaaaagcagg ctccgcggcc gcccccttca ccatggcttc ctctatgctc
     8041 tcttccgcta ctatggttgc ctctccggct caggccacta tggtcgctcc tttcaacgga
     8101 cttaagtcct ccgctgcctt cccagccacc cgcaaggcta acaacgacat tacttccatc
     8161 acaagcaacg gcggaagagt taactgcatg caggtgtggc ctccgattgg aaagaagaag
     8221 tttgagactc tctcttacct tcctgacctt accgattccg aaaagcttga aatggcttct
     8281 caacagggtg agactatgtc aggaccattg catatcggtc taatcccaac agttggacct
     8341 tacttacttc ctcacattat cccaatgcta caccaaactt tccctaaact tgaaatgtac
     8401 cttcacgagg cacagactca tcaactattg gcccagttag atagtggtaa acttgattgt
     8461 gtcatactag ctttggtaaa ggaatccgag gcattcatag aagtgcctct tttcgatgaa
     8521 cctatgcttt tagcaatcta tgaagatcac ccatgggcca atagagaatg cgttccaatg
     8581 gcagatttgg ccggtgagaa acttctaatg cttgaggatg gacactgctt gagggatcaa
     8641 gctatgtccg ccggttacaa ttcagataac gtctatatca tggccgacaa acagaagaat
     8701 ggtataaagg ccaacttcaa aattagacat aacgtggaag atggttccgt acaactagca
     8761 gaccattatc aacagaacac cccaatcggt gacggacctg ttttacttcc agataaccac
     8821 tacctatctt ttcagtctgt cctatccaaa gacccaaatg agaaaaggga tcacatggtg
     8881 ttgcttgagt tcgtaaccgc tgccggtatt actcttggta tggatgagct atacaatgta
     8941 gacggaggta gtggaggtac aggttccaag ggagaagagt tgttcacagg agttgtccca
     9001 atattagtag aacttgatgg tgacgtgaac ggtcacaaat tttcagtctc cggagaaggt
     9061 gagggtgacg caacctacgg aaaactaaca ttgaaattaa tctgtacaac tggaaaactt
     9121 cctgtcccat ggccaacatt agtaacaacc ttaggttacg gtttaaagtg cttcgcaaga
     9181 tatcctgacc atatgaagca acatgacttc ttcaagagtg ctatgcctga gggatatgtg
     9241 caagagagga ctatattctt caaagacgac ggtaattata aaactagggc agaagtgaag
     9301 tttgagggag atactttggt gaatagaata gagcttaaag gtatcggatt caaagaggac
     9361 ggaaatattc ttggacacaa gctagagtac aacggaaccg gattttgctt cgaagccggt
     9421 gctgacgagg atactcactt cagggctaca tcactagaaa ccttgaggaa catggtagcc
     9481 gttggaagtg gaataacttt gttaccagca cttgccgtgc caccagagag gaagagggat
     9541 ggtgtagttt atcttccatg cattaaacca gagccaagaa ggaccattgg attggtctac
     9601 aggccaggtt caccattgag atcaaggtat gaacagttag cagaggctat aagagccagg
     9661 atggatggtc actttgataa ggttttgaag caagcagtgt gaaagggtgg gcgcgccgac
     9721 ccagctttct tgtacaaagt ggttgataac agcgcttaga gctcgaattt ccccgatcgt
     9781 tcaaacattt ggcaataaag tttcttaaga ttgaatcctg ttgccggtct tgcgatgatt
     9841 atcatataat ttctgttgaa ttacgttaag catgtaataa ttaacatgta atgcatgacg
     9901 ttatttatga gatgggtttt tatgattaga gtcccgcaat tatacattta atacgcgata
     9961 gaaaacaaaa tatagcgcgc aaactaggat aaattatcgc gcgcggtgtc atctatgtta
    10021 ctagatcggg aattagcttc atcaacgcaa gacatgcgca cgaccgtctg acaggagagg
    10081 aatttccgac gagcacagaa aggacttgct cttggacgta ggcctatttc tcaggcacat
    10141 gtatcaagtg ttcggacgtg ggttttcgat ggtgtatcag ccgccgccaa ctgggagatg
    10201 aggaggcttt cttggggggc agtcagcagt tcatttcaca agacagagga acttgtaagg
    10261 agatgcactg atttatcttg gcgcaaacca gcaggacgaa ttagtgggaa tagcccgcga
    10321 atatctaagt tatgcctgtc ggcatgagca gaaacttcca attcgaaaca gtttggagag
    10381 gttgtttttg ggcatacctt ttgttagtca gcctctcgat tgctcatcgt cattacacag
    10441 taccgaagtt tgatcgatct agtaacatag atgacaccgc gcgcgataat ttatcctagt
    10501 ttgcgcgcta tattttgttt tctatcgcgt attaaatgta taattgcggg actctaatca
    10561 taaaaaccca tctcataaat aacgtcatgc attacatgtt aattattaca tgcttaacgt
    10621 aattcaacag aaattatatg ataatcatcg caagaccggc aacaggattc aatcttaaga
    10681 aactttattg ccaaatgttt gaacgatctg cttcgacgca ctccttcttt actccaccat
    10741 ctcgtcctta ttgaaaacgt gggtagcacc aaaacgaatc aagtcgctgg aactgaagtt
    10801 accaatcacg ctggatgatt tgccagttgg attaatcttg cctttccccg catgaataat
    10861 attgatgaat gcatgcgtga ggggtatttc gattttggca atagctgcaa ttgccgcgac
    10921 atcctccaac gagcataatt cttcagaaaa atagcgatgt tccatgttgt cagggcatgc
    10981 atgatgcacg ttatgaggtg acggtgctag gcagtattcc ctcaaagttt catagtcagt
    11041 atcatattca tcattgcatt cctgcaagag agaattgaga cgcaatccac acgctgcggc
    11101 aaccttccgg cgttcgtggt ctatttgctc ttggacgttg caaacgtaag tgttggatcc
    11161 cggtcggcat ctactctatt cctttgccct cggacgagtg ctggggcgtc ggtttccact
    11221 atcggcgagt acttctacac agccatcggt ccagacggcc gcgcttctgc gggcgatttg
    11281 tgtacgcccg acagtcccgg ctccggatcg gacgattgcg tcgcatcgac cctgcgccca
    11341 agctgcatca tcgaaattgc cgtcaaccaa gctctgatag agttggtcaa gaccaatgcg
    11401 gagcatatac gcccggagcc gcggcgatcc tgcaagctcc ggatgcctcc gctcgaagta
    11461 gcgcgtctgc tgctccatac aagccaacca cggcctccag aagaagatgt tggcgacctc
    11521 gtattgggaa tccccgaaca tcgcctcgct ccagtcaatg accgctgtta tgcggccatt
    11581 gtccgtcagg acattgttgg agccgaaatc cgcgtgcacg aggtgccgga cttcggggca
    11641 gtcctcggcc caaagcatca gctcatcgag agcctgcgcg acggacgcac tgacggtgtc
    11701 gtccatcaca gtttgccagt gatacacatg gggatcagca atcgcgcata tgaaatcacg
    11761 ccatgtagtg tattgaccga ttccttgcgg tccgaatggg ccgaacccgc tcgtctggct
    11821 aagatcggcc gcagcgatcg catccatggc ctccgcgacc ggctgcagaa cagcgggcag
    11881 ttcggtttca ggcaggtctt gcaacgtgac accctgtgca cggcgggaga tgcaataggt
    11941 caggctctcg ctgaattccc caatgtcaag cacttccgga atcgggagcg cggccgatgc
    12001 aaagtgccga taaacataac gatctttgta gaaaccatcg gcgcagctat ttacccgcag
    12061 gacatatcca cgccctccta catcgaagct gaaagcacga gattcttcgc cctccgagag
    12121 ctgcatcagg tcggagacgc tgtcgaactt ttcgatcaga aacttctcga cagacgtcgc
    12181 ggtgagttca ggctttttca tatcggggtc gtcctctcca aatgaaatga acttccttat
    12241 atagaggaag ggtcttgcga aggatagtgg gattgtgcgt catcccttac gtcagtggag
    12301 atatcacatc aatccacttg ctttgaagac gtggttggaa cgtcttcttt ttccacgatg
    12361 ctcctcgtgg gtgggggtcc atctttggga ccactgtcgg cagaggcatc ttgaacgata
    12421 gcctttcctt tatcgcaatg atggcatttg taggtgccac cttccttttc tactgtcctt
    12481 ttgatgaagt gacagatagc tgggcaatgg aatccgagga ggtttcccga tattaccctt
    12541 tgttgaaaag tctcaatagc cctttggtct tctgagactg tatctttgat attcttggag
    12601 tagacgagag tgtcgtgctc caccatgttg acggatctct aggacgcgtc ctagaagcta
    12661 attcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta
    12721 atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg
    12781 atcgcccttc ccaacagttg cgcagcctga atggcgcccg ctcctttcgc tttcttccct
    12841 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
    12901 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgattt gggtgatggt
    12961 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
    13021 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcgggctat
    13081 tcttttgatt tataagggat tttgccgatt tcggaaccac catcaaacag gattttcgcc
    13141 tgctggggca aaccagcgtg gaccgcttgc tgcaactctc tcagggccag gcggtgaagg
    13201 gcaatcagct gttgcccgtc tcactggtga aaagaaaaac caccccagta cattaaaaac
    13261 gtccgcaatg tgttattaag ttgtctaagc gtcaatttgt ttacaccaca atatatcctg
    13321 ccaccagcca gccaacagct ccccgaccgg cagctcggca caaaatcacc actcgataca
    13381 ggcagcccat cagtccggga cggcgtcagc gggagagccg ttgtaaggcg gcagactttg
    13441 ctcatgttac cgatgctatt cggaagaacg gcaactaagc tgccgggttt gaaacacgga
    13501 tgatctcgcg gagggtagca tgttgattgt aacgatgaca gagcgttgct gcctgtgatc
    13561 aaatatcatc tccctcgcag agatccgaat tatcagcctt cttattcatt tctcgcttaa
    13621 ccgtgacagg ctgtcgatct tgagaactat gccgacataa taggaaatcg ctggataaag
    13681 ccgctgagga agctgagtgg cgctatttct ttagaagtga acgttgacga tatcaactcc
    13741 cctatccatt gctcaccgaa tggtacaggt cggggacccg aagttccgac tgtcggcctg
    13801 atgcatcccc ggctgatcga ccccagatct ggggctgaga aagcccagta aggaaacaac
    13861 tgtaggttcg agtcgcgaga tcccccggaa ccaaaggaag taggttaaac ccgctccgat
    13921 caggccgagc cacgccaggc cgagaacatt ggttcctgta ggcatcggga ttggcggatc
    13981 aaacactaaa gctactggaa cgagcagaag tcctccggcc gccagttgcc aggcggtaaa
    14041 ggtgagcaga ggcacgggag gttgccactt gcgggtcagc acggttccga acgccatgga
    14101 aaccgccccc gccaggcccg ctgcgacgcc gacaggatct agcgctgcgt ttggtgtcaa
    14161 caccaacagc gccacgcccg cagttccgca aatagccccc aggaccgcca tcaatcgtat
    14221 cgggctacct agcagagcgg cagagatgaa cacgaccatc agcggctgca cagcgcctac
    14281 cgtcgccgcg accccgcccg gcaggcggta gaccgaaata aacaacaagc tccagaatag
    14341 cgaaatatta agtgcgccga ggatgaagat gcgcatccac cagattcccg ttggaatctg
    14401 tcggacgatc atcacgagca ataaacccgc cggcaacgcc cgcagcagca taccggcgac
    14461 ccctcggcct cgctgttcgg gctccacgaa aacgccggac agatgcgcct tgtgagcgtc
    14521 cttggggccg tcctcctgtt tgaagaccga cagcccaatg atctcgccgt cgatgtaggc
    14581 gccgaatgcc acggcatctc gcaaccgttc agcgaacgcc tccatgggct ttttctcctc
    14641 gtgctcgtaa acggacccga acatctctgg agctttcttc agggccgaca atcggatctc
    14701 gcggaaatcc tgcacgtcgg ccgctccaag ccgtcgaatc tgagccttaa tcacaattgt
    14761 caattttaat cctctgttta tcggcagttc gtagagcgcg ccgtgcgtcc cgagcgatac
    14821 tgagcgaagc aagtgcgtcg agcagtgccc gcttgttcct gaaatgccag taaagcgctg
    14881 gctgctgaac ccccagccgg aactgacccc acaaggccct agcgtttgca atgcaccagg
    14941 tcatcattga cccaggcgtg ttccaccagg ccgctgcctc gcaactcttc gcaggcttcg
    15001 ccgacctgct cgcgccactt cttcacgcgg gtggaatccg atccgcacat gaggcggaag
    15061 gtttccagct tgagcgggta cggctcccgg tgcgagctga aatagtcgaa catccgtcgg
    15121 gccgtcggcg acagcttgcg gtacttctcc catatgaatt tcgtgtagtg gtcgccagca
    15181 aacagcacga cgatttcctc gtcgatcagg acctggcaac gggacgtttt cttgccacgg
    15241 tccaggacgc ggaagcggtg cagcagcgac accgattcca ggtgcccaac gcggtcggac
    15301 gtgaagccca tcgccgtcgc ctgtaggcgc gacaggcatt cctcggcctt cgtgtaatac
    15361 cggccattga tcgaccagcc caggtcctgg caaagctcgt agaacgtgaa ggtgatcggc
    15421 tcgccgatag gggtgcgctt cgcgtactcc aacacctgct gccacaccag ttcgtcatcg
    15481 tcggcccgca gctcgacgcc ggtgtaggtg atcttcacgt ccttgttgac gtggaaaatg
    15541 accttgtttt gcagcgcctc gcgcgggatt ttcttgttgc gcgtggtgaa cagggcagag
    15601 cgggccgtgt cgtttggcat cgctcgcatc gtgtccggcc acggcgcaat atcgaacaag
    15661 gaaagctgca tttccttgat ctgctgcttc gtgtgtttca gcaacgcggc ctgcttggcc
    15721 tcgctgacct gttttgccag gtcctcgccg gcggtttttc gcttcttggt cgtcatagtt
    15781 cctcgcgtgt cgatggtcat cgacttcgcc aaacctgccg cctcctgttc gagacgacgc
    15841 gaacgctcca cggcggccga tggcgcgggc agggcagggg gagccagttg cacgctgtcg
    15901 cgctcgatct tggccgtagc ttgctggacc atcgagccga cggactggaa ggtttcgcgg
    15961 ggcgcacgca tgacggtgcg gcttgcgatg gtttcggcat cctcggcgga aaaccccgcg
    16021 tcgatcagtt cttgcctgta tgccttccgg tcaaacgtcc gattcattca ccctccttgc
    16081 gggattgccc cgactcacgc cggggcaatg tgcccttatt cctgatttga cccgcctggt
    16141 gccttggtgt ccagataatc caccttatcg gcaatgaagt cggtcccgta gaccgtctgg
    16201 ccgtccttct cgtacttggt attccgaatc ttgccctgca cgaataccag cgaccccttg
    16261 cccaaatact tgccgtgggc ctcggcctga gagccaaaac acttgatgcg gaagaagtcg
    16321 gtgcgctcct gcttgtcgcc ggcatcgttg cgccacatct aggtactaaa acaattcatc
    16381 cagtaaaata taatatttta ttttctccca atcaggcttg atccccagta agtcaaaaaa
    16441 tagctcgaca tactgttctt ccccgatatc ctccctgatc gaccggacgc agaaggcaat
    16501 gtcataccac ttgtccgccc tgccgcttct cccaagatca ataaagccac ttactttgcc
    16561 atctttcaca aagatgttgc tgtctcccag gtcgccgtgg gaaaagacaa gttcctcttc
    16621 gggcttttcc gtctttaaaa aatcatacag ctcgcgcgga tctttaaatg gagtgtcttc
    16681 ttcccagttt tcgcaatcca catcggccag atcgttattc agtaagtaat ccaattcggc
    16741 taagcggctg tctaagctat tcgtataggg acaatccgat atgtcgatgg agtgaaagag
    16801 cctgatgcac tccgcataca gctcgataat cttttcaggg ctttgttcat cttcatactc
    16861 ttccgagcaa aggacgccat cggcctcact catgagcaga ttgctccagc catcatgccg
    16921 ttcaaagtgc aggacctttg gaacaggcag ctttccttcc agccatagca tcatgtcctt
    16981 ttcccgttcc acatcatagg tggtcccttt ataccggctg tccgtcattt ttaaatatag
    17041 gttttcattt tctcccacca gcttatatac cttagcagga gacattcctt ccgtatcttt
    17101 tacgcagcgg tatttttcga tcagtttttt caattccggt gatattctca ttttagccat
    17161 ttattatttc cttcctcttt tctacagtat ttaaagatac cccaagaagc taattataac
    17221 aagacgaact ccaattcact gttccttgca ttctaaaacc ttaaatacca gaaaacagct
    17281 ttttcaaagt tgttttcaaa gttggcgtat aacatagtat cgacggagcc gattttgaaa
    17341 ccacaattat gggtgatgct gccaacttac tgatttagtg tatgatggtg tttttgaggt
    17401 gctccagtgg cttctgtgtc tatcagctgt ccctcctgtt cagctactga cggggtggtg
    17461 cgtaacggca aaagcaccgc cggacatcag cgctatctct gctctcactg ccgtaaaaca
    17521 tggcaactgc agttcactta caccgcttct caacccggta cgcaccagaa aatcattgat
    17581 atggccatga atggcgttgg atgccgggca acagcccgca ttatgggcgt tggcctcaac
    17641 acgattttac gtcacttaaa aaactcaggc cgcagtcggt aacctcgcgc atacagccgg
    17701 gcagtgacgt catcgtctgc gcggaaatgg acgaacagtg gggctatgtc ggggctaaat
    17761 cgcgccagcg ctggctgttt tacgcgtatg acagtctccg gaagacggtt gttgcgcacg
    17821 tattcggtga acgcactatg gcgacgctgg ggcgtcttat gagcctgctg tcaccctttg
    17881 acgtggtgat atggatgacg gatggctggc cgctgtatga atcccgcctg aagggaaagc
    17941 tgcacgtaat cagcaagcga tatacgcagc gaattgagcg gcataacctg aatctgaggc
    18001 agcacctggc acggctggga cggaagtcgc tgtcgttctc aaaatcggtg gagctgcatg
    18061 acaaagtcat cgggcattat ctgaacataa aacactatca ataagttgga gtcattaccc
    18121 aattatgata gaatttacaa gctataaggt tattgtcctg ggtttcaagc attagtccat
    18181 gcaagttttt atgctttgcc cattctatag atatattgat aagcgcgctg cctatgcctt
    18241 gccccctgaa atccttacat acggcgatat cttctatata aaagatatat tatcttatca
    18301 gtattgtcaa tatattcaag gcaatctgcc tcctcatcct cttcatcctc ttcgtcttgg
    18361 tagcttttta aatatggcgc ttcatagagt aattctgtaa aggtccaatt ctcgttttca
    18421 tacctcggta taatcttacc tatcacctca aatggttcgc tgggtttatc gcacccccga
    18481 acacgagcac ggcacccgcg accactatgc caagaatgcc caaggtaaaa attgccggcc
    18541 ccgccatgaa gtccgtgaat gccccgacgg ccgaagtgaa gggcaggccg ccacccaggc
    18601 cgccgccctc actgcccggc acctggtcgc tgaatgtcga tgccagcacc tgcggcacgt
    18661 caatgcttcc gggcgtcgcg ctcgggctga tcgcccatcc cgttactgcc ccgatcccgg
    18721 caatggcaag gactgccagc gctgccattt ttggggtgag gccgttcgcg gccgaggggc
    18781 gcagcccctg gggggatggg aggcccgcgt tagcgggccg ggagggttcg agaagggggg
    18841 gcacccccct tcggcgtgcg cggtcacgcg cacagggcgc agccctggtt aaaaacaagg
    18901 tttataaata ttggtttaaa agcaggttaa aagacaggtt agcggtggcc gaaaaacggg
    18961 cggaaaccct tgcaaatgct ggattttctg cctgtggaca gcccctcaaa tgtcaatagg
    19021 tgcgcccctc atctgtcagc actctgcccc tcaagtgtca aggatcgcgc ccctcatctg
    19081 tcagtagtcg cgcccctcaa gtgtcaatac cgcagggcac ttatccccag gcttgtccac
    19141 atcatctgtg ggaaactcgc gtaaaatcag gcgttttcgc cgatttgcga ggctggccag
    19201 ctccacgtcg ccggccgaaa tcgagcctgc ccctcatctg tcaacgccgc gccgggtgag
    19261 tcggcccctc aagtgtcaac gtccgcccct catctgtcag tgagggccaa gttttccgcg
    19321 aggtatccac aacgccggcg gccgcggtgt ctcgcacacg gcttcgacgg cgtttctggc
    19381 gcgtttgcag ggccatagac ggccgccagc ccagcggcga gggcaaccag cccgg
//
