LOCUS       pAav-MDM2-Cter_PQS2_3xHA      6877 bp    DNA     circular UNA 
DEFINITION  Ligation of HR2 cut into vector cut for HR2
FEATURES             Location/Qualifiers
     Region          1..141
                     /label=ITR
     iDNA            152..1171
                     /label="MDM2 5' HOMOLOGY REGION"
     CDS             1175..1222
                     /label="F10 FRT SITE"
     old_sequence    1224..1241
                     /label=OTC
                     /label="Tryptic context"
     CDS             1242..1277
                     /label=PQS2
     old_sequence    1278..1295
                     /label=OTC
                     /label="Tryptic context"
     CDS             1296..1385
                     /label=3xHA
     old_sequence    1413..1446
                     /label=LoxP
     5'clip          1454..1961
                     /label="PGK promotor"
                     /label="mPGK promoter"
     5'clip          1972..2775
                     /label="Neomycin Phosphotransferase"
     old_sequence    3062..3095
                     /label=LoxP
     iDNA            3101..4127
                     /label="MDM2 3' HOMOLOGY REGION"
     old_sequence    4140..4280
                     /label=ITR
ORIGIN
        1 CCTGCAGGCA GCTGCGCGCT CGCTCGCTCA CTGAGGCCGC CCGGGCAAAG CCCGGGCGTC
       61 GGGCGACCTT TGGTCGCCCG GCCTCAGTGA GCGAGCGAGC GCGCAGAGAG GGAGTGGCCA
      121 ACTCCATCAC TAGGGGTTCC TGCGGCCGCA AACGCGTAAA GGGCCCTGGT AAGGGTGAAA
      181 AAAATGGGTG TACTTACTCT ACTTTAGGAG AGACTGTTAC ATATTGACCT TTAAAAATCT
      241 GTTTTGTGTA TAATTGAGAC ATATAACGTG ATGTTTTGAC ACACATGTAA ATAGTGAAAT
      301 GATCACTACA GTCAAGCAAA TTAATGTAAT CCATTATCTT GAATAGTTAA CCTTTCTTGT
      361 GTGTATGGTT AAAATACCTA AAATCTATTC TTTTAGTAAA TTTCCAGTAT ACCAATATAT
      421 AACTAACTAT AGTCCTCATG CTGTTTACAG TGACTATTTT ATATAAAACA TGAAACACTG
      481 AATATTGAGC CCTATGATAT ACTTTACCTT AGACATAGCA AAGTTGCTAG CATTCCTGTG
      541 ACTGAGCAGT TAAAGGGTTA CAGAAACTGA CTGTGTGTCT TATTTCATTG AAGGACTATT
      601 GGAAATGCAC TTCATGCAAT GAAATGAATC CCCCCCTTCC ATCACATTGC AACAGATGTT
      661 GGGCCCTTCG TGAGAATTGG CTTCCTGAAG ATAAAGGGAA AGATAAAGGG GAAATCTCTG
      721 AGAAAGCCAA ACTGGAAAAC TCAACACAAG CTGAAGAGGG CTTTGATGTT CCTGATTGTA
      781 AAAAAACTAT AGTGAATGAT TCCAGAGAGT CATGTGTTGA GGAAAATGAT GATAAAATTA
      841 CACAAGCTTC ACAATCACAA GAAAGTGAAG ACTATTCTCA GCCATCAACT TCTAGTAGCA
      901 TTATTTATAG CAGCCAAGAA GATGTGAAAG AGTTTGAAAG GGAAGAAACC CAAGACAAAG
      961 AAGAGAGTGT GGAATCTAGT TTGCCCCTTA ATGCCATTGA ACCTTGTGTG ATTTGTCAAG
     1021 GTCGACCTAA AAATGGTTGC ATTGTCCATG GCAAAACAGG ACATCTTATG GCCTGCTTTA
     1081 CATGTGCAAA GAAGCTAAAG AAAAGGAATA AGCCCTGCCC AGTATGTAGA CAACCAATTC
     1141 AAATGATTGT GCTAACTTAT TTCCCCGAAT TCGGGAAGTT CCTATTCCGA AGTTCCTATT
     1201 CTCTAGAAAG TATAGGAACT TCAGTGGCCG AGGCTTATAG AGGCCTGGGA GCTTCTCCAG
     1261 GAATCGGTGC TGGAAGATTC CTGGAGACAG AAAACTACCC ATACGATGTT CCAGATTACG
     1321 CTGGCTATCC CTATGACGTC CCGGACTATG CAGGAAGCTA TCCCTATGAC GTTCCAGATT
     1381 ACGCTGGCGA GCCCGAGGCT TGAAAGCTTC GGATAACTTC GTATAGCATA CATTATACGA
     1441 AGTTATCGCG CCCTACCGGG TAGGGGAGGC GCTTTTCCCA AGGCAGTCTG GAGCATGCGC
     1501 TTTAGCAGCC CCGCTGGGCA CTTGGCGCTA CACAAGTGGC CTCTGGCCTC GCACACATTC
     1561 CACATCCACC GGTAGGCGCC AACCGGCTCC GTTCTTTGGT GGCCCCTTCG CGCCACCTTC
     1621 TACTCCTCCC CTAGTCAGGA AGTTCCCCCC CGCCCCGCAG CTCGCGTCGT GCAGGACGTG
     1681 ACAAATGGAA GTAGCACGTC TCACTAGTCT CGTGCAGATG GACAGCACCG CTGAGCAATG
     1741 GAAGCGGGTA GGCCTTTGGG GCAGCGGCCA ATAGCAGCTT TGGCTCCTTC GCTTTCTGGG
     1801 CTCAGAGGCT GGGAAGGGGT GGGTCCGGGG GCGGGCTCAG GGGCGGGCTC AGGGGCGGGG
     1861 CGGGCGCCCG AAGGTCCTCC GGAAGCCCGG CATTCTGCAC GCTTCAAAAG CGCACGTCTG
     1921 CCGCGCTGTT CTCCTCTTCC TCATCTCCGG GCCTTTCGAC CTGCAGCCAA TATGGGATCG
     1981 GCCATTGAAC AAGATGGATT GCACGCAGGT TCTCCGGCCG CTTGGGTGGA GAGGCTATTC
     2041 GGCTATGACT GGGCACAACA GACAATCGGC TGCTCTGATG CCGCCGTGTT CCGGCTGTCA
     2101 GCGCAGGGGC GCCCGGTTCT TTTTGTCAAG ACCGACCTGT CCGGTGCCCT GAATGAACTG
     2161 CAGGACGAGG CAGCGCGGCT ATCGTGGCTG GCCACGACGG GCGTTCCTTG CGCAGCTGTG
     2221 CTCGACGTTG TCACTGAAGC GGGAAGGGAC TGGCTGCTAT TGGGCGAAGT GCCGGGGCAG
     2281 GATCTCCTGT CATCTCACCT TGCTCCTGCC GAGAAAGTAT CCATCATGGC TGATGCAATG
     2341 CGGCGGCTGC ATACGCTTGA TCCGGCTACC TGCCCATTCG ACCACCAAGC GAAACATCGC
     2401 ATCGAGCGAG CACGTACTCG GATGGAAGCC GGTCTTGTCA ATCAGGATGA TCTGGACGAA
     2461 GAGCATCAGG GGCTCGCGCC AGCCGAACTG TTCGCCAGGC TCAAGGCGCG CATGCCCGAC
     2521 GGCGAGGATC TCGTCGTGAC CCATGGCGAT GCCTGCTTGC CGAATATCAT GGTGGAAAAT
     2581 GGCCGCTTTT CTGGATTCAT CGACTGTGGC CGGCTGGGTG TGGCGGATCG CTATCAGGAC
     2641 ATAGCGTTGG CTACCCGTGA TATTGCTGAA GAGCTTGGCG GCGAATGGGC TGACCGCTTC
     2701 CTCGTGCTTT ACGGTATCGC CGCTCCCGAT TCGCAGCGCA TCGCCTTCTA TCGCCTTCTT
     2761 GACGAGTTCT TCTGAGGGGA TCAATTCTCT AGAGCTCGCT GATCAGCCTC GACTGTGCCT
     2821 TCTAGTTGCC AGCCATCTGT TGTTTGCCCC TCCCCCGTGC CTTCCTTGAC CCTGGAAGGT
     2881 GCCACTCCCA CTGTCCTTTC CTAATAAAAT GAGGAAATTG CATCGCATTG TCTGAGTAGG
     2941 TGTCATTCTA TTCTGGGGGG TGGGGTGGGG CAGGACAGCA AGGGGGAGGA TTGGGAAGAC
     3001 AATAGCAGGC ATGCTGGGGA TGCGGTGGGC TCTATGGCTT CTGAGGCGGA AAGAACCAGC
     3061 TATAACTTCG TATAGCATAC ATTATACGAA GTTATAGTAA TCGATAACTC GAGTAGTTGA
     3121 CCTGTCTATA AGAGAATTAT ATATTTCTAA CTATATAACC CTAGGAATTT AGACAACCTG
     3181 AAATTTATTC ACATATATCA AAGTGAGAAA ATGCCTCAAT TCACATAGAT TTCTTCTCTT
     3241 TAGTATAATT GACCTACTTT GGTAGTGGAA TAGTGAATAC TTACTATAAT TTGACTTGAA
     3301 TATGTAGCTC ATCCTTTACA CCAACTCCTA ATTTTAAATA ATTTCTACTC TGTCTTAAAT
     3361 GAGAAGTACT TGGTTTTTTT TTTTCTTAAA TATGTATATG ACATTTAAAT GTAACTTATT
     3421 ATTTTTTTTG AGACCGAGTC TTGCTCTGTT ACCCAGGCTG GAGTGCAGTG GCGTGATCTT
     3481 GGCTCACTGC AAGCTCTGCC TCCCGGGTTC GCACCATTCT CCTGCCTCAG CCTCCCAATT
     3541 AGCTTGGCCT ACAGTCATCT GCCACCACAC CTGGCTAATT TTTTGTACTT TTAGTAGAGA
     3601 CAGGGTTTCA CCGTGTTAGC CAGGATGGTC TCGATCTCCT GACCTCGTGA TCCGCCCACC
     3661 TCGGCCTCCC AAAGTGCTGG GATTACAGGC ATGAGCCACC GCGTCCGGCC TAAATGTCAC
     3721 TTAGTACCTT TGATATAAAG AGAAAATGTG TGAAAGATTT AGTTTTTTGT TTTTTTGTTT
     3781 GTTTGTTTGT TTGTTTGTTT TGAGATGAGT CTCTCTGTCG CCCAGGCTGG AGTGCAGTGT
     3841 CATGATCTAG CAGTCTCCGC TTCCCGGGTT CAAGCCATTC TCCTGGCTCA GCCTCTGGAG
     3901 CAGCTGGGAT TACAGGCATG CACCACCATG CCCAGCTAAT TTTTGTATTT TTAGTAGAGA
     3961 TAGGGTTTCA CCATGTTGGC CAGGCTGGTC ACGAACTCCT GACCTCAAGT GAGGTCACCC
     4021 GCCTCGGCCT CCCGAAGTGC TGGGATTGCA GATGTGAGCC ACCATGTCCA GCCAAGAATT
     4081 AGTATTTAAA TTTTAGATAC TCTTTTTTTT TTTGGTACCA AGCTAGCAAA AGCGGCCGCA
     4141 GGAACCCCTA GTGATGGAGT TGGCCACTCC CTCTCTGCGC GCTCGCTCGC TCACTGAGGC
     4201 CGGGCGACCA AAGGTCGCCC GACGCCCGGG CTTTGCCCGG GCGGCCTCAG TGAGCGAGCG
     4261 AGCGCGCAGC TGCCTGCAGG GGCGCCTGAT GCGGTATTTT CTCCTTACGC ATCTGTGCGG
     4321 TATTTCACAC CGCATACGTC AAAGCAACCA TAGTACGCGC CCTGTAGCGG CGCATTAAGC
     4381 GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC
     4441 GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT
     4501 CTAAATCGGG GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA
     4561 AAACTTGATT TGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC
     4621 CCTTTGACGT TGGAGTCCAC GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA
     4681 CTCAACCCTA TCTCGGGCTA TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGCCTAT
     4741 TGGTTAAAAA ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTTTAACAA AATATTAACG
     4801 TTTACAATTT TATGGTGCAC TCTCAGTACA ATCTGCTCTG ATGCCGCATA GTTAAGCCAG
     4861 CCCCGACACC CGCCAACACC CGCTGACGCG CCCTGACGGG CTTGTCTGCT CCCGGCATCC
     4921 GCTTACAGAC AAGCTGTGAC CGTCTCCGGG AGCTGCATGT GTCAGAGGTT TTCACCGTCA
     4981 TCACCGAAAC GCGCGAGACG AAAGGGCCTC GTGATACGCC TATTTTTATA GGTTAATGTC
     5041 ATGATAATAA TGGTTTCTTA GACGTCAGGT GGCACTTTTC GGGGAAATGT GCGCGGAACC
     5101 CCTATTTGTT TATTTTTCTA AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC
     5161 TGATAAATGC TTCAATAATA TTGAAAAAGG AAGAGTATGA GTATTCAACA TTTCCGTGTC
     5221 GCCCTTATTC CCTTTTTTGC GGCATTTTGC CTTCCTGTTT TTGCTCACCC AGAAACGCTG
     5281 GTGAAAGTAA AAGATGCTGA AGATCAGTTG GGTGCACGAG TGGGTTACAT CGAACTGGAT
     5341 CTCAACAGCG GTAAGATCCT TGAGAGTTTT CGCCCCGAAG AACGTTTTCC AATGATGAGC
     5401 ACTTTTAAAG TTCTGCTATG TGGCGCGGTA TTATCCCGTA TTGACGCCGG GCAAGAGCAA
     5461 CTCGGTCGCC GCATACACTA TTCTCAGAAT GACTTGGTTG AGTACTCACC AGTCACAGAA
     5521 AAGCATCTTA CGGATGGCAT GACAGTAAGA GAATTATGCA GTGCTGCCAT AACCATGAGT
     5581 GATAACACTG CGGCCAACTT ACTTCTGACA ACGATCGGAG GACCGAAGGA GCTAACCGCT
     5641 TTTTTGCACA ACATGGGGGA TCATGTAACT CGCCTTGATC GTTGGGAACC GGAGCTGAAT
     5701 GAAGCCATAC CAAACGACGA GCGTGACACC ACGATGCCTG TAGCAATGGC AACAACGTTG
     5761 CGCAAACTAT TAACTGGCGA ACTACTTACT CTAGCTTCCC GGCAACAATT AATAGACTGG
     5821 ATGGAGGCGG ATAAAGTTGC AGGACCACTT CTGCGCTCGG CCCTTCCGGC TGGCTGGTTT
     5881 ATTGCTGATA AATCTGGAGC CGGTGAGCGT GGGTCTCGCG GTATCATTGC AGCACTGGGG
     5941 CCAGATGGTA AGCCCTCCCG TATCGTAGTT ATCTACACGA CGGGGAGTCA GGCAACTATG
     6001 GATGAACGAA ATAGACAGAT CGCTGAGATA GGTGCCTCAC TGATTAAGCA TTGGTAACTG
     6061 TCAGACCAAG TTTACTCATA TATACTTTAG ATTGATTTAA AACTTCATTT TTAATTTAAA
     6121 AGGATCTAGG TGAAGATCCT TTTTGATAAT CTCATGACCA AAATCCCTTA ACGTGAGTTT
     6181 TCGTTCCACT GAGCGTCAGA CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT
     6241 TTTCTGCGCG TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT
     6301 TTGCCGGATC AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG
     6361 ATACCAAATA CTGTCCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA
     6421 GCACCGCCTA CATACCTCGC TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT
     6481 AAGTCGTGTC TTACCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG
     6541 GGCTGAACGG GGGGTTCGTG CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG
     6601 AGATACCTAC AGCGTGAGCT ATGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC
     6661 AGGTATCCGG TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA
     6721 AACGCCTGGT ATCTTTATAG TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT
     6781 TTGTGATGCT CGTCAGGGGG GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA
     6841 CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT CACATGT
//
